ASSESS THE EFFECTIVENESS OF STRUCTURED TEACHING PROGRAMMED REGARDING COLLECTION OF CORD BLOOD FOR STEM CELL THERAPY AT BRAM HOSPITAL OF RAIPUR

The stem cells, derived from the cord blood are hematopoietic stem cells. These have immense potential in curing blood related disorders like blood cancers, thalassemia etc. These can be useful in treating tissue related disorders of heart, bone, spinal cord etc. Stem cells are characterized by the ability to renew through mitotic cell division and differentiate into a diverse range of specialized cell types. Stem Cells are the basic building blocks of the body and have the potential to replenish other cells and give rise to number of tissues which constitute different organs. To conduct the study, 60 antenatal mothers are selected the age range of subjects was 21 to 40 years. The need for the study arises after knowing those antenatal mothers were unaware and having inadequate knowledge regarding cord blood collection for stem cell therapy. The above facts created an interest to conduct a structured teaching programme to assess its effectiveness on knowledge and attitude of antenatal mother on cord blood collection for stem cell therapy. The pretest, the mean score of knowledge is 13.2 mean % is 50.7, & attitude is 31.58, mean % 63.16, the post test the mean score of knowledge is 18.1, mean % is 69.65. Attitude 38.05, mean % 76.1.

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A REPORT ON STEM CELLS AND THEIR APPLICATIONS

10.36106/ijar/2010867 ◽

2020 ◽

pp. 1-2

Author(s):

Shantha A R

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Building Blocks ◽

Cell Types ◽

The Body ◽

Cell Therapies ◽

Undifferentiated Cells ◽

The Cost

Stem cells are the building blocks of life. They have remarkable potential to regenerate and develop into many different cell types in the body during early life and growth. They are also a class of undifferentiated cells that are able to be differentiated into specialized cells types. Stem cells are characterized by certain features such as totipotency, pluripotency, multipotency, oligopotent and unipotency. The history of stem cell research had an embryonic beginning in the mid 1800s with the discovery that few cells could generate other cells. In the 1900s the first stem cells were discovered when it was found that cells generate blood cells. Nowadays, stem cell therapy is under research and till now, a very few stem cell therapies have been regarded as safe and successful. It is also found that stem cell therapy cast a number of side effects too. The cost of the procedure too is expensive and is not easily affordable.

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A study to Evaluate the effectiveness of Structured Teaching Program regarding collection of cord blood for stem cell therapy in terms of knowledge among staff nurses in selected hospitals at Bangalore

Asian Journal of Nursing Education and Research ◽

10.5958/2349-2996.2018.00003.4 ◽

2018 ◽

Vol 8 (1) ◽

pp. 07

Author(s):

Vivitha S. Pinto ◽

N. P Pushpaveni

Keyword(s):

Stem Cell ◽

Cell Therapy ◽

Cord Blood ◽

Stem Cell Therapy ◽

Staff Nurses ◽

Teaching Program ◽

Structured Teaching

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Stemness specificity of epithelial cells – application of cell and tissue technology in regenerative medicine

Medical Journal of Cell Biology ◽

10.2478/acb-2018-0018 ◽

2018 ◽

Vol 6 (3) ◽

pp. 114-119 ◽

Cited By ~ 2

Author(s):

Magdalena Rojewska ◽

Małgorzata Popis ◽

Maurycy Jankowski ◽

Dorota Bukowska ◽

Paweł Antosik ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Regenerative Medicine ◽

Cell Therapy ◽

Stem Cell Therapy ◽

The Body ◽

Current Evidence ◽

Epithelial Tissue

AbstractStem cells are cells that have the potential to replicate and/or differentiate, becoming any tissue. This process could be theoretically repeated indefinitely and can be used to create or fix damaged parts any organ. There are many in vivo factors that cause stem cells to replicate and differentiate. Many of these interactions and mechanisms are still unknown. In vitro models have been successful in inducing stem cells to differentiate into the desired lineage using controlled methods. Recently, epithelial tissue has been successfully created using scaffolds on which stem cells are grown in vitro and then transplanted into the host. This transition creates significant problems. This is because in vitro -grown stem cells or stem cell-derived tissues are created in an isolated environment where virtually every aspect can be monitored and controlled. In vivo monitoring and controlling is significantly more difficult for a plethora of reasons. Cells in the body are constantly exposed to many signals and molecules which affect them. Many of the mechanisms behind these interactions and reactions are known but many others are not. As the corpus of knowledge grows, stem cells become closer to being applied in a clinical setting. In this paper, we review the current evidence on stem cell therapy in regenerative medicine and some of the challenges this field faces.

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Is Stem Cell a Curer or an Obstruction?

Molecular and Cellular Biomedical Sciences ◽

10.21705/mcbs.v1i1.12 ◽

2017 ◽

Vol 1 (1) ◽

pp. 17

Author(s):

Siska Damayanti ◽

Rina Triana ◽

Angliana Chouw ◽

Nurrani Mustika Dewi

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Embryonic Stem ◽

Cell Types ◽

Tumor Formation ◽

The Body ◽

Negative Effects ◽

Tissue Repair And Regeneration

Introduction: Each cell in human body is assigned with a specialized function to perform. Before a cell becomes specialized, it is a stem cell. Stem cell research and therapy is progressing dramatically these days. Stem cell therapy holds enormous treatment potential for many diseases which currently have no or limited therapeutic options. Unfortunately, this potential also comes with side-effects. In this review, the positive and negative effects of regulation of stem cells will be explained.Content: Stem cells are undifferentiated cells that have potential to develop into many different cell types in the body during early life and growth. The type of stem cells are embryonic stem cells, induced pluripotent stem cells, somatic stem cells, foetal stem cells and mesenchymal stem cells. Stem cell transplantation is one form of stem cell therapy, it comes with different sources, and those are autologous and allogenic transplantation stem cells. In an autologous transplant, a patient’s own blood-forming stem cells are collected, meanwhile in an allogeneic transplant, a person’s stem cells are replaced with new stem cells obtained from a donor or from donated umbilical cord blood.Summary: Its abilities to maintain undifferentiated phenotype, self-renewing and differentiate itself into specialized cells, give rise to stem cell as a new innovation for the treatment of various diseases. In the clinical setting, stem cells are being explored in various conditions, such as in tissue repair and regeneration and autoimmune diseases therapy. But along with its benefit, stem cell therapy also holds some harm. It is known that the treatment using stem cell for curing and rehabilitation has the risk in tumor formation.

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AB0870 PAIN AND FUNCTIONAL OUTCOME OF PERIPHERAL BLOOD DERIVED STEM CELLS IN PRIMARY OSTEOARTHRITIS OF KNEE AT LOW RESOURCES SETTING: A PHASE II PILOT RCT

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2020-eular.4740 ◽

2020 ◽

Vol 79 (Suppl 1) ◽

pp. 1741.2-1741

Author(s):

M. R. Khasru

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Peripheral Blood ◽

Pain Reduction ◽

Cartilage Thickness ◽

Functional Improvement ◽

Pilot Rct ◽

The Mean

Background:Osteoarthritis (OA) of the knee is one of the main causes of musculoskeletal disability. Osteoarthritis is now often considered as organ failure. Because of limitations in the effectiveness of conventional management options, alternative possibilities such as cell based therapies are approaching into vogueObjectives:To assess the effectiveness of peripheral bold derived stem cells (PBSCs) therapy for primary OA knee.Methods:This phase-II pilot RCT was conducted after the IRB ethical clearance. Patients attending PM&R department of BSMMU having KL grade III osteoarthritis knee those fulfilled the selection criteria were considered as the sample. All respondents were divided to into two groups by using randomization technique. In control group, 15 respondents received standard care for Knee Osteoarthritis. In PBSCs group, 15 cases received with single dose autologous peripheral blood derived stem cells (PBSCs). Stem cells were harvested using G-CSF 30MU and CD34 stem cells were collected through apheresis. Quality was ensured measuring cell viability and surface antigen. All respondents were assessed before treatment and at week 4, week 12 and week 24 for pain reduction and for functional improvement using Visual Analogue Scale (VAS 0-10 cm) and validated Bengali WOMAC questionnaire. Joint sonography was done before treatment, at 12 week and 24 week after treatment commencement.Results:Female were 60% and 67% in control and PBSCs group respectively. The mean age was 56.3±6.7 and 55.8±7.3 year in control and PBSCs. After 12 weeks of stem cell therapy, pain reduction and functional significant improvement was observed in both groups. However, after 24 weeks further significant pain reduction, functional improvement was observed in PBSCs group. Moreover, the cartilage thickness measured by high frequency ultrasound and the mean cartilage thickness was increased by 0.14 mm from the baseline among PBSCs group.Conclusion:PBSCs is promising in patients with primary OA knee. Further large scale research is solicited.References:[1]Dubey, N., Mishra, V., Dubey, R., Syed-Abdul, S., Wang, J., Wang, P. and Deng, W. (2018). Combating Osteoarthritis through Stem Cell Therapies by Rejuvenating Cartilage: A Review. Stem Cells International, 2018, pp.1-13.[2]Jevotovsky, D., Alfonso, A., Einhorn, T. and Chiu, E. (2018). Osteoarthritis and stem cell therapy in humans: a systematic review. Osteoarthritis and Cartilage, 26(6), pp.711-729.[3]Wang, A., Feng, Y., Jia, H., Zhao, M. and Yu, H. (2019). Application of mesenchymal stem cell therapy for the treatment of osteoarthritis of the knee: A concise review. World Journal of Stem Cells, 11(4), pp.222-235.Acknowledgments:Department of Transfusion Medicine, BSMMUDisclosure of Interests:None declared

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Regulation of Stem Cell-Based Research in India in Comparison with the US, EU and other Asian Countries: Current Issues and Future Perspectives

Current Stem Cell Research & Therapy ◽

10.2174/1574888x15666200402134750 ◽

2020 ◽

Vol 15 (6) ◽

pp. 492-508

Author(s):

Jobin Jose ◽

Teena George ◽

Aaron M. Thomas

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

Heart Diseases ◽

The Body ◽

Regulatory Agencies ◽

Cell Therapies ◽

The Us ◽

Regenerative Medicines

Stem cell therapy is applicable for repair and replacement of damaged cells and tissues. Apart from transplanting cells to the body, the stem cell therapy directs them to grow new and healthy tissues. Stem cells in the area of regenerative medicines hold tremendous promise that may help to regenerate the damaged tissues and heal various diseases like multiple sclerosis, heart diseases, Parkinson’s disease, and so on. To prove the safety, efficacy, and for the requirement of a licence for manufacturing and sale, all the stem cell therapies should pass the required criteria and undergo certain examinations of the regulatory agencies. The regulatory authorities review the manufacturing procedures of products to assure its purity and potency. This review summarizes the comparative critical evaluations of existing regulations and developments on the stem cells research in India, USA, EU and Asian regions and also discusses the challenges that have to be overcome and the important points that should be understood to position India as a source of the perspective nation in stem cells around the world.

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Neuroblastoma and stem cell therapy – An updated review

CNS & Neurological Disorders - Drug Targets ◽

10.2174/1871527319666201102100911 ◽

2020 ◽

Vol 19 ◽

Author(s):

Manoj Kumar Gupta ◽

Suresh Mallepalli ◽

Amooru G Damu ◽

Ramakrishna Vadde

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Cell Therapy ◽

Stem Cell Therapy ◽

The Body ◽

Sequencing Technologies ◽

Stem Cell Rescue ◽

Undifferentiated Cells ◽

Risk Patients ◽

Near Future

Background: Neuroblastoma (NBM) is the second leading pediatric cancer that develops from the precursors of the sympathetic nervous system. To date, surgery, chemotherapy, and radiation serve as the first line treatment against NBM in high-risk patients. However, few of these approaches have severe side effects. Recently, numerous studies have also reported that high chemotherapy doses, along with stem cell rescue, improvise event-free survival in patients. Objectives: In this review, the authors attempted to discuss the pathogenesis associated with NBM and how stem cell therapy can be employed for the treatment of NBM. Conclusions: Stem cells are a group of multipotent undifferentiated cells that are capable of producing all cells in a particular tissue, organ, or organism. They have an endogenous self-renewal property. This property is tightly modulated for the normal homeostasis within the body. However, the failure of this process leads to carcinogenesis, including NBM. As these properties are modulated via various intrinsic as well as extrinsic pathways, the arrest of these pathways via various drugs may help in controlling various carcinomas, including NBM. Recently, stem cells used diagnosis and therapy is widely for the NBM treatments. Nevertheless, most of the studies conducted to date are mainly designed on bulk-cell analysis, which in turn provides little information about the population of cells. Thus, the authors believe that, by employing single-cell RNA sequencing technologies and computational approaches, we can unmask the tumor heterogeneity in NBM in a more comprehensive way. In the near future, this information will be highly useful for the identification of biomarkers and treatment associated with NBM in humans.

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Augmented Cord Blood Stem Cell Collection, Making Adult Single Cord Transplant a Reality?

Blood ◽

10.1182/blood.v122.21.5447.5447 ◽

2013 ◽

Vol 122 (21) ◽

pp. 5447-5447

Author(s):

Robert A. Dracker

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Cord Blood ◽

Mesenchymal Cell ◽

Cell Yield ◽

Blood Collection ◽

Stem Cell Source ◽

Cord Blood Stem Cell ◽

Blood Stem Cells ◽

Cord Blood Collection

For over 25 years, cord blood stem cell collection and clinical use as a viable stem cell source for hematologic reconstitution has continued to receive increased acceptance and more diverse applications. Recently, the utilization of cellular sub-types and differentiated populations from cord blood has been employed for a variety of conditions including those resulting from cellular and organ dysfunction or damage. Unfortunately, the usefulness of cord blood derived stem cells for these varieties of applications has been limited by the stem cell number constraints of a typical cord blood collection, as compared to other sources of stem cells such as bone marrow, peripheral blood and tissue-derived stem cells. Clearly, cord blood stem cells have been recognized to have unique advantageous characteristics, such as the ready availability of a discarded, almost limitless resource; being intrinsically and relatively free of pathogens and prior infectious burdens and sequelae and demonstrating significant pluripotential capability while demonstrating improved graft versus host tolerance. Attempts to improve the useful number of cord blood derived stem cells for a particular indication have included combined multiple cord transplants, a variety of methods to expand stem cell populations and techniques to increase the stem cell yield from a placenta and umbilical cord. Methodologies to increase cord blood collection volume and cell yield have included placental expression, perfusion techniques and placental and cord tissue maceration and cellular separation. Although some of these techniques have demonstrated potential limited usefulness, they have lacked reproducibility, routine procedural practicality and have intrinsic specific risks including infection potential, cellular byproducts and the use of non-approved pharmaceuticals. If possible, the collection of sufficient number of cord blood stem cells from a single cord blood collection, which would be suitable for either a pediatric or adult transplantation, would be of great value. Additionally, the value of the use of an abundant, low cost stem cell source such as cord blood for general use for either hematopoietic or mesenchymal cell application for all potential recipients in need is obvious. However, the procurement of such an invaluable resource needs to be reliable, reproducible and simplistic by nature, avoiding specialized equipment, facilities or expertise at the time of collection. This presented technology represents such a procedure which can result in the collection of a two part stem cell product which can provide for the needs of both an adult stem cell transplant requirements as well and serve as a biologic resource for other stem cell applications such as mesenchymal cell indications. This described technique allows for the standard primary cord blood collection, followed by the placental administration of physiologic vasodilators and stem cell releasing factors in a solution which is both nutritive and cell supportive in nature. The second augmented collection immediately follows which results in a combined and increased stem cell volume that is two to three times the typical mononuclear cell yield. Transportation of a two part collection to the processing facility is standard in nature and requires no subsequent modification in the mononuclear separation procedure prior to storage. The percent CD34+ cell population is significantly elevated in the second collection volume, demonstrating effective mobilization of marginating placental stem cells. Additionally, the procedure is technically reliable and reproducible by any experienced collector and requires only a minor modification in a standard cord blood collection procedure without the use of cumbersome equipment or materials. Disclosures: No relevant conflicts of interest to declare.

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