Community of Hydrocarbon-Oxidizing Bacteria in Petroleum Products on the example of Ts-1 Aviation Fuel and AI-95 Gasoline

2021 ◽  
Vol 37 (1) ◽  
pp. 54-68
Author(s):  
E.S. Lobakova ◽  
G.A. Dolnikova ◽  
E.A. Ivanova ◽  
D.A. Sanjieva ◽  
A.A. Burova ◽  
...  
Keyword(s):  
Rhodococcus Erythropolis ◽  
Basic Research ◽  
Petroleum Products ◽  
The State ◽  
Aviation Fuel ◽  
Bacterial Strains ◽  
16S Rrna Sequence ◽  
State Assignment ◽  
Microbial Biofilms ◽  
Microbial Potential

It has been shown that the studied petroleum products (kerosene and gasoline) contain microflocules of heterogeneous microbial biofilms, the cells of which are integrated into a polymer matrix containing acidic polysaccharides. Thirteen bacterial strains were microbiologically isolated from petroleum products, and their taxonomy was identified by the 16S rRNA sequence. Kerosene was characterized by a diverse bacterial composition including the following genera: Sphingobacterium, Alcaligenes, Rhodococcus and Deinococcus, while gasoline bacterial community included only two genera: Bacillus and Paenibacillus. Representatives of the Deinococcus genera capable of growing on the hydrocarbons were isolated from fuels for the first time. The strains isolated from gasoline (Bacillus safensis Bi13 and Bacillus sp. Bi14) proved to be the most effective biodegraders of all n-alkanes, isoalkanes, cycloalkanes, alkenes and aromatic hydrocarbons, whereas the kerosene strain Rhodococcus erythropolis Bi6 effectively decomposed n-alkanes and trimethylbenzene. Both types of petroleum products contained hydrocarbon-oxidizing communities, some members of which were more active in the biodegradation of hydrocarbons, while others were capable of producing biosurfactants and had either emulsifying activity (Deinococcus sp. Bi7) or cell wall hydrophobicity (Sphingobacterium sp. Bi5 from kerosene; Bacillus pumilus Bi12 from gasoline) significantly higher than the average level. The indicated properties of the studied strains make them promising for use in bioremediation. biodegradation, petroleum products, hydrocarbon-oxidizing bacteria, bio-surfactants The work was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (topic no. 10.5422.2017/8.9.). Investigation of microbial potential in the use hydrocarbons was supported by the Russian Foundation for Basic Research (RFBR), contract no. 18-29-05067. Physicochemical research was performed within the framework of the state assignment to the TIPS RAS

Method for Obtaining Daedaleopsis confragosa K-1326 Extracts with Antitumor Activity

2019 ◽  
Vol 35 (1) ◽  
pp. 68-72
Author(s):  
T.V. Teplyakova ◽  
E.A. Vyazovaya ◽  
E.D. Danilenko
Keyword(s):  
Antitumor Activity ◽  
Cell Line ◽  
Carcinoma Cell ◽  
The State ◽  
Carcinoma Cell Line ◽  
Epidermoid Carcinoma ◽  
State Assignment ◽  
Therapeutic Drugs ◽  
Prevention And Treatment ◽  
Epidermoid Carcinoma Cell Line

A method for extraction and fractionation of proteins and polysaccharides from the biomass of the basidial fungus Daedaleopsis confragosa K-1326, having antitumor activity, shown on the epidermoid carcinoma cell line A431 and the cells of melanoma В16, has been developed. This method allows for reproducible production of series of preparations and can be used to obtain preparations from other basidiomycetes. The results obtained in this work show the promising prospects for the development and use of drugs from basidiomycetes for treatment of cancer. Basidiomycetes, Daedaleopsis confragosa, antitumor activity, fractionation, extraction The work was performed under the State Assignment «Development of preventive and therapeutic drugs based on basidiomycetes for the prevention and treatment of influenza with pandemic potential».

scholarly journals Pyrrolnitrin Biosynthesis From Rhizospheric Serratia Spp. With Antifungal Activity and Binding Interactions of PrnF With Ligands

2020 ◽  
Author(s):  
Shraddha P. Pawar ◽  
Ambalal B. Chaudhari
Keyword(s):  
Antifungal Activity ◽  
Phytopathogenic Fungi ◽  
Bacterial Strains ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Protein Model ◽  
Stenotrophomonas Rhizophila ◽  
K 12 ◽  
Layer Chromatography

Abstract Pyrrolnitrin (PRN) from rhizobacteria displays a key role in biocontrol of phytopathogenic fungi in rhizospheric soil. Therefore, different rhizospheric soils were investigated for the prevalence of PRN producer in minimal salt (MS) medium containing tryptophan (0.2 M NaCl; pH 8) using three successive enrichments. Of 12% isolates, only five bacterial strains had shown PRN secretion, screened with Thin Layer Chromatography (Rf 0.8) and antifungal activity (27 mm) against phytopathogen. The phenetic and 16S rRNA sequence revealed the close affiliation of isolates (KMB, M-2, M-11, TW3, and TO2) to Stenotrophomonas rhizophila (KY800458), Enterobacter spp. (KY800455), Brevibacillus parabrevis (KY800454), Serratia marcescens (KY800456) and Serratia nemtodiphila (KY800457). Purified compound from isolates was characterised using UV, IR, HPLC, LCMS and GCMS as PRN. However, BLASTn hit of prn gene sequences from both Serratia species showed 99% similarity with NADPH dependent FMN reductase component (prnF). The homology protein model of prnF was developed from translated sequence of S. marcescens TW3 with chromate reductase of Escherichia coli K-12. Docking with FMN and NADPH was performed. The study demonstrated the possible role of prnF NADPH dependent FMN reductases in prnD for supply of reduced flavin in rhizobacterial strain of Serratia spp. which may pave a way to understand PRN production.

scholarly journals VIR collecting missions in 2020

VAVILOVIA ◽  
2021 ◽  
Vol 3 (4) ◽  
pp. 41-47
Author(s):  
T. M. Ozerskaya ◽  
Yu. V. Ukhatova
Keyword(s):  
Genetic Resources ◽  
Plant Genetic Resources ◽  
The State ◽  
Herbarium Specimens ◽  
Research Project ◽  
State Assignment ◽  
Regions Of Russia

The article presents a brief overview of expeditionary surveys of the territory of Russia by VIR scientists in 2020 within the framework of the State Assignment for the research project No. 0481-2020-0001 “Ensuring the preservation and replenishment of the collection of plant genetic resources”. In 2020, with the support from budgetary and non-budgetary sources, VIR scientists participated in 8 collecting missions to various regions of Russia (Krasnodar Territory, Adygea, Karelia, including Valaam Island, Murmansk and Arkhangelsk provinces, the Solovetsky Islands, Yakutia, and Khabarovsk Territory). The seeds, cuttings, live plants, and herbarium specimens collected in 2020 for replenishing the VIR collection totaled 580 samples.

scholarly journals Biodegradation of the xenobiotic organic disulphide 4,4′-dithiodibutyric acid by Rhodococcus erythropolis strain MI2 and comparison with the microbial utilization of 3,3′-dithiodipropionic acid and 3,3′-thiodipropionic acid

Microbiology ◽  
2010 ◽  
Vol 156 (4) ◽  
pp. 1221-1233 ◽  
Author(s):  
Jan Hendrik Wübbeler ◽  
Nadine Bruland ◽  
Milena Wozniczka ◽  
Alexander Steinbüchel
Keyword(s):  
Carbon Source ◽  
Succinic Acid ◽  
Hydrogen Sulphide ◽  
Rhodococcus Erythropolis ◽  
Building Blocks ◽  
Bacterial Strains ◽  
Sulphur Compounds ◽  
Promising Alternative ◽  
Microbial Utilization ◽  
Broad Variety

Application of the non-toxic 3,3′-thiodipropionic acid (TDP) and 3,3′-dithiodipropionic acid (DTDP) as precursors for the microbial production of polythioesters (PTEs), a class of biologically persistent biopolymers containing sulphur in the backbone, was successfully established previously. However, synthesis of PTEs containing 4-mercaptobutyrate (4MB) as building blocks could not be achieved. The very harmful 4MB is not used as a PTE precursor or as the carbon source for growth by any known strain. As a promising alternative, the harmless oxidized disulfide of two molecules of 4MB, 4,4′-dithiodibutyric acid (DTDB), was employed for enrichments of bacterial strains capable of biodegradation. Investigation of novel precursor substrates for PTEs and comparison of respective strains growing on TDP, DTDP and DTDB as sole carbon source was accomplished. A broad variety of bacteria capable of using one of these organic sulphur compounds were isolated and compared. TDP and DTDP were degraded by several strains belonging to different genera, whereas all DTDB-utilizing strains were affiliated to the species Rhodococcus erythropolis. Transposon mutagenesis of R. erythropolis strain MI2 and screening of 7500 resulting mutants yielded three mutants exhibiting impaired growth on DTDB. Physiological studies revealed production of volatile hydrogen sulphide and accumulation of significant amounts of 4MB, 4-oxo-4-sulphanylbutanoic acid and succinic acid in the culture supernatants. Based on this knowledge, a putative pathway for degradation of DTDB was proposed: DTDB could be cleaved into two molecules of 4MB, followed by an oxidation yielding 4-oxo-4-sulphanylbutanoic acid. A putative desulphydrase probably catalyses the abstraction of sulphur, thereby generating succinic acid and hydrogen sulphide.

Unity and diversity turko-mongol world: Kultobe settlement as a crossroads of religions and cultures

2021 ◽  
Vol 46 (1) ◽  
pp. 186-195
Author(s):  
A. Khazbulatov ◽  
◽  
L. Nekhvyadovich ◽  
Zh. Shaigozova ◽  
◽  
...  
Keyword(s):  
The State ◽  
Archaeological Research ◽  
State University ◽  
State Assignment ◽  
Unique Material ◽  
Architectural Structures ◽  
Material Evidence ◽  
Diversity Study ◽  
Research Institute

The culture and art of the Turkic-Mongolian world is one of the deepest subjects of the humanities, which again and again take the attention of researchers. The historical vicissitudes, which was formed over many centuries, contributed to spread Zoroastrianism, Manichaeism, Buddhism, Christianity and Islam in the Great Steppe. We can see it in unique art artifacts and architectural structures. This article is devoted to unity and diversity study of the Turkic-Mongolian world, based on the example of the Kultobe settlement, which is presented by the authors as the oldest peculiar crossroads of religions and cultures. The Kultobe settlement, during scientific years and archaeological research by the Kazakh Research Institute of Culture, truly unique material evidence (architectural objects and artifacts) was discovered, which allows us to speak of this unique monument of archeology as one of the “crossroads” of religions and cultures in the Turkic-Mongolian world. The Kultobe settlement and later Yasi, a spiritual and religious place, developed -Turkic Sufism. The famous Sufi and poet K.A.Yasawi lived and preached here. The work was prepared under the state assignment of the Altai State University, project №748715Ф.99.1.ББ97АА00002 “The Turkic-Mongolian world of the “Greater Altai”: unity and diversity in history and modernity.”

Rett syndrome: The state of clinical and basic research, and future perspectives

2011 ◽  
Vol 33 (8) ◽  
pp. 627-631 ◽  
Author(s):  
Toyojiro Matsuishi ◽  
Yushiro Yamashita ◽  
Tomoyuki Takahashi ◽  
Shinichiro Nagamitsu
Keyword(s):  
Rett Syndrome ◽  
Basic Research ◽  
The State ◽  
Future Perspectives

THE GIPPSLAND OIL AND GAS FIELDS—FOUR YEARS OF PRODUCTION AND UTILISATION

1973 ◽  
Vol 13 (1) ◽  
pp. 166
Author(s):  
M. A. Stratton
Keyword(s):  
Natural Gas ◽  
Social Life ◽  
Oil And Gas ◽  
Petroleum Industry ◽  
Petroleum Products ◽  
The State ◽  
Oil Field ◽  
Oil Fields ◽  
Gas Field ◽  
Paper Mills

The discovery by the partnership of Esso Exploration and Production Australia Inc. and Hematite Petroleum Pty Ltd during the past eight years of the natural gas and crude oil fields off the east Victorian coast has often been compared to that of gold in the State in the 1850's in its impact .on the economic, industrial and social life of the community.To date the amount spent in the State on the discovery and overall development of these fields is approximately $600 million. The value of oil and gas recovered over the period of nearly four years since production commenced in 1969 and distributed and utilised by various means to 31 December 1972, amounts to about $500 million. In addition the value of refined products from Victoria's three refineries and items produced by industrial processes through the use of natural gas and petroleum products as fuels, amount to many more millions of dollars. The total impact on Victoria in one form or another could, if measured in monetary value, he equivalent to about $1200 million-all in the course of about eight years.Other States have also benefited. The building of tankers, barges, tugs and work boats and the modification of refineries in New South Wales and Queensland, have probably cost in the region of $200 million whilst indirectly the success of the Gippsland oil and gas discoveries has spurred other explorers to step up the search in many areas and, as far as natural gas is concerned, with considerable success.The speed and efficiency with which the four gas and oil fields developed to date were brought into production, the necessary treatment plants erected, the pipelines laid and distribution facilities organised; and with which the gas industry changed over to the new fuel and refineries modified their processes to use indigenous crudes have, by world standards, been exceptional. From the time the first gas field-Barracouta, was found in February 1965 until the last oil field in the program -Kingfish came fully on stream late in 1971, less than seven years elapsed.During that time Victorian fuel patterns underwent vast changes. Today over 95% of all gas consumers are using natural gas and about 70% of crude processed by local refineries comes from the Gippsland Basin. The significance of natural gas in particular is demonstrated by a 41% increase in gas sales in Victoria in 1971/72 over the previous twelve months and this trend is expected to accelerate as a result of recent arrangements for the supply of large volumes of this fuel to industrial plants including paper mills, cement works and an alumina smelter.Also of major significance to the State has been the development of the port of Western Port where the loading of tankers and LPG carriers has resulted in it becoming the State's second busiest port. Of less immediate impact but still of great value in the long term, has been the building of better roads and facilities needed to service the installations and the emergence of many valuable skills in the petroleum industry which will make easier the task of future development of new fields and facilities in Victoria and other parts of Australia.

scholarly journals Qualifying the T-2 Toxin-Degrading Properties of Seven Microbes with Zebrafish Embryo Microinjection Method

Toxins ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 460
Author(s):  
Edina Garai ◽  
Anita Risa ◽  
Emese Varga ◽  
Mátyás Cserháti ◽  
Balázs Kriszt ◽  
...  
Keyword(s):  
Sublethal Effects ◽  
Degradation Products ◽  
Rhodococcus Erythropolis ◽  
Bacterial Strains ◽  
Cell Stage ◽  
Bacterial Metabolites ◽  
Mycotoxin Degradation

T-2 mycotoxin degradation and detoxification efficiency of seven bacterial strains were investigated with zebrafish microinjection method in three steps ((1) determination of mycotoxin toxicity baseline, (2) examination of bacterial metabolites toxicity, (3) identification of degradation products toxicity). Toxicity of T-2 was used as a baseline of toxic effects, bacterial metabolites of strains as control of bacterial toxicity and degradation products of toxin as control of biodegradation were injected into one-cell stage embryos in the same experiment. The results of in vivo tests were checked and supplemented with UHPLC-MS/MS measurement of T-2 concentration of samples. Results showed that the Rhodococcus erythropolis NI1 strain was the only one of the seven tested (R. gordoniae AK38, R. ruber N361, R. coprophilus N774, R. rhodochrous NI2, R. globerulus N58, Gordonia paraffinivorans NZS14), which was appropriated to criteria all aspects (bacterial and degradation metabolites of strains caused lower toxicity effects than T-2, and strains were able to degrade T-2 mycotoxin). Bacterial and degradation metabolites of the NI1 strain caused slight lethal and sublethal effects on zebrafish embryos at 72- and 120-h postinjection. Results demonstrated that the three-step zebrafish microinjection method is well-suited to the determination and classification of different bacterial strains by their mycotoxin degradation and detoxification efficiency.

Sign in / Sign up

Export Citation Format

Share Document