Invitro antibacterial potentials of sensitive plant (Mimosa pudica) and bitter lemon (Momordica charantia) leaf extracts and synthetic antibiotics against some bacteria isolates of Clarias gariepinus.

The study evaluated nutritional and organoleptic properties of Clarias gariepinus pre-treated with aqueous leave extracts of Ocimum gratissimum and Vernonia amygdalina before processing. Thirty-six (36) C. gariepinus (live weight = 1.2± 0.2kg) samples were procured and processed for the study. Fish samples were divided into three parts of twelve fish each. Each part was grouped into three treatments having four fish in each group using a completely randomized design. Treatment one (T1) had fish immersed in 5% brine without herbal extract (control). In Treatment two (T2), fish were soaked in mixture of 5% brine and 5% O. gratissium (scent leaf) extracts while Treatment three (T3) had fish soaked in mixture of 5% brine and 5% V. amygdalina (bitter leaf) extracts all for 30 minutes. Treated fish samples were smoke-dried using charcoal kiln for 5 hours at temperature of 80 – 90° C. Samples were then cooled under ambient condition (30+3oC) for 4 hours. The smoke-dried fish samples were labeled according to treatments and stored for 7 days under aseptic condition. Organoleptic properties of the samples were conducted at every 24-hour of storage using a 9-point hedonic scale. Mean values of proximate composition of samples were significantly different (p<0.05). Protein content (70.01%) in T3 was higher than 68.31% and 66.62% in T2 and T1 respectively. Similarly, fat (5.12%) and ash (4.68%) levels for T3 were higher than 4.85% and 4.46% fat with 4.36% and 3.98% ash contents for T2 and T1 respectively. Results of mean scores for each organoleptic property decreased significantly (p<0.05) during storage period with no consistent trend in score pattern. Keywords: Clarias gariepinus, extract pretreatment, Ocimum gratissimum, organoleptic, Vernonia amygdalina.

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Toxicity of aquaeous leaf extracts of Gongronema latifolium on behaviourial and haematological characteristics of Clarias gariepinus (Burchell, 1822) juveniles

Journal of Aquatic Sciences ◽

10.4314/jas.v35i1.3 ◽

2020 ◽

Vol 35 (1) ◽

pp. 19-28

Author(s):

J.N. Nwakpa ◽

E.E Oti ◽

M.U. Agim

Keyword(s):

Blood Cell ◽

Clarias Gariepinus ◽

Colour Change ◽

Threshold Value ◽

Quality Parameters ◽

Feed Additive ◽

Pack Cell Volume ◽

Leaf Extracts ◽

Mean Values ◽

Gongronema Latifolium

A static bioassay experiment was set up to investigate effects of acute (96-hrLC50) concentrations of aqueous leaf extracts of Gongronema latifolium on behaviourial and haematological characteristics of Clarias gariepinus (Burchell, 1822) juveniles. Evaluation of toxicity of leaf extracts at concentrations of: 0, 10, 25, 50, 75, and 100mg/l was done at various time intervals on the fish. A total of 180 catfish juveniles with average initial weight of 8.37±1.36g and total length of 12.10±2.45cm were used. The fish were distributed among the treatments on complete randomized design. Each treatment had three replicates. The results showed that aqueous leaf extracts were toxic to experimental fish. There was an observed erratic movement, colour change from black to ash-brown and dark coloured belly of treated fish. There were slight changes in water quality parameters, with no significant effect (p>0.05) on the tolerance level of fish on G. latifolium leaf extracts. Mean values of 96-hrLC50 were 90.14mg/l and 2.78mg/l for the upper and lower limits while the threshold value was 0.26mg/l. Phyto-chemical analysis of the leaves showed constituents of flavonoids, saponins, tannins, anthrocyanide, alkaloids and glycosides. Effects of aqueous extracts on haematological indices revealed increasing values of pack cell volume, haemoglobin, white blood cell, red blood cell, mean corpuscular volume, mean corpuscular heamoglobin and mean corpuscular heamoglobin concentration counts with extract concentration. Thus, G. latifolium could be used as feed additive for improved fish production at concentration below 70%. Keywords: Gongronema latifolium, leaf extracts, behaviour, haematology, Clarias gariepinus

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Efficacy of Aloe vera Gel and Water-leaf Extracts for Removal of Egg Adhesiveness during Artificial Propagation of African Catfish (Clarias gariepinus, Burchel 1822)

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2019/v3i330037 ◽

2019 ◽

pp. 1-13

Author(s):

S. J. Fawehinmi ◽

O. T. Adebayo ◽

O. K. Gbadamosi

Keyword(s):

Plant Extracts ◽

Polynomial Regression ◽

Clarias Gariepinus ◽

Aloe Vera ◽

Urea Solution ◽

African Catfish ◽

Leaf Extracts ◽

Egg Hatching ◽

Aloe Vera Gel ◽

Immersion Period

The paper aims to study the best immersion period and concentration of the plant extracts under study (water-leaf and Aloe vera gel) that can efficiently remove egg adhesiveness of Clarias gariepinus. Data generated were subjected to Multivariate Analysis of Variance Test, Tukey multiple range tests was used as a follow up procedure. Third order Polynomial regression analysis was then used to determine the best concentration and immersion period that neutralizes adhesiveness in eggs of C. gariepinus. The experiment was carried out at The Teaching and Research Fish Farm, Federal University of Technology, Akure, between October 2018 to December 2018. One male and one female C. gariepinus brood stock weighing 1.0 kg and 1.3 kg, respectively were used for the experiment. Three different concentrations (1, 3, and 5)% of the plant extracts were used. 2 g of urea diluted in 4 g NaCl/L of water was used as reference de-adhesion agent, while water without urea solution nor plant extracts was used as control. The fish eggs were rinsed with the solutions at different durations of 1, 3 and 5 minutes. Each concentration and rinsing time was recorded in duplicates. Results of the study showed that there were no significant differences (p=0.05) in the non-adhesive egg hatching of eggs immersed in Aloe vera gel and Urea solution. Number of non-adhesive egg and hatching increased in waterleaf extract which was significantly different (P=0.05) from eggs immersed in Aloe vera gel and water but not significantly different from urea solution. Therefore this study shows that waterleaf extract at 1% concentration and 1 minute immersion period can efficiently remove egg adhesiveness and increase hatching in C. gariepinus.

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The antimalaria effect of Momordica charantia L. and Mirabilis jalapa leaf extracts using animal model

Journal of Medicinal Plants Research ◽

10.5897/jmpr2016.6046 ◽

2016 ◽

Vol 10 (24) ◽

pp. 344-350 ◽

Cited By ~ 3

Author(s):

Olufunke Christy Akanji ◽

C Mojisola Cyril Olutayo ◽

O Taiwo Elufioye ◽

Omowumi Ola Ogunsusi

Keyword(s):

Animal Model ◽

Momordica Charantia ◽

Leaf Extracts ◽

Mirabilis Jalapa

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Studies on comparative antimicrobial activities of Aerva lanata and Momordica charantia leaf extracts

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2020.120519 ◽

2020 ◽

pp. 561-564

Author(s):

Nagula Shilpika ◽

Byagari Teena Keerthana ◽

Banka Srinivas ◽

Gampa Tulja Rani ◽

Ganga Maithili

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antimicrobial Activities ◽

Momordica Charantia ◽

Leaf Extracts ◽

Plate Method ◽

Methanolic Extracts ◽

Aerva Lanata

Screening and comparison of antimicrobial action of leaf extract of Aerva lanata and Momordica charantia. Ethyl acetate and methanolic extracts of leaves of plants were screened for antimicrobial activity using the cup plate method and the spread plate method against gram positive and gram negative reference organisms (Bacillus substilis and Escherichia coli).The standard antibacterial agent used for reference is Choramphenicol and the results were calculated as zone of inhibition. Methanolic extract showed comparatively broader and better antimicrobial spectrum than ethyl acetate extract in selected plants. Plant extracts showed dose dependent action, results were similar to the action of the standard Choramphenicol. Extracts of Aerva lanata and Momordica charantia demonstrated antimicrobial activity on tested microorganisms. Methanolic extracts showed higher antimicrobial potential than ethyl acetate extract. Aerva lanata extracts showed better response than Momordica charantia extracts in the cup plate method antibacterial activity with Bacillus substilis and Escherichia coli.

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TOXICITY STUDY OF PARE LEAF EXTRACTS (MOMORDICA CHARANTIA) TO CULEX PIPIENS MOSQUITO LARVAE

Journal of Islamic Pharmacy ◽

10.18860/jip.v2i2.4506 ◽

2017 ◽

Vol 2 (2) ◽

pp. 13

Author(s):

Rahmi Annisa ◽

Muhammad Amir ◽

Hadi Kuncoro

Keyword(s):

Ethyl Acetate ◽

Crude Extract ◽

Culex Pipiens ◽

Ethyl Acetate Fraction ◽

Momordica Charantia ◽

Hexane Fraction ◽

Test Results ◽

Potential Toxicity ◽

Leaf Extracts ◽

Toxic Concentration

This study aims to determine the yield, toxicity, and toxic concentration of crude extracts of ethanol, n-hexane fraction extract, ethyl acetate fraction extract and n-butanol fractions extract pare leaf (Momordica charantiaL.) larvae Culex pipiens. The method used to determine the toxicity of extract larvasida test larvae Culex pipiens to obtain the LC99 values obtained using the analysis of Reed and Muench. Extraction and fractionation processis known to yield a ethanol crude extract obtained 8.86%, n-hexane fraction extract of 1%, ethyl acetate fraction extract 1.80% and n-butanol fractions extract of 0.80% and the toxicity obtained test results that pare leaf (Momordica charantia L.) have the potential toxicity larvae larvae of Culex pipiens as indicated by the value by Lethality Concrentation 99 % (LC99) in the crude extract ethanol 7416.52 ppm, n-hexane fraction extract of 2564.48 ppm, ethyl acetate fraction extract 2702.09 ppm and n-butanol fractions extract of 2665.63 ppm. Keywords: Pare leaf (Momordica charantia L.), toxicity, larvae Culex pipiens

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