Comparison of the phytochemicals and antimicrobial activity of leaf extracts of Calotropis procera, Momordica charantia and Allium ascalonicum

Screening and comparison of antimicrobial action of leaf extract of Aerva lanata and Momordica charantia. Ethyl acetate and methanolic extracts of leaves of plants were screened for antimicrobial activity using the cup plate method and the spread plate method against gram positive and gram negative reference organisms (Bacillus substilis and Escherichia coli).The standard antibacterial agent used for reference is Choramphenicol and the results were calculated as zone of inhibition. Methanolic extract showed comparatively broader and better antimicrobial spectrum than ethyl acetate extract in selected plants. Plant extracts showed dose dependent action, results were similar to the action of the standard Choramphenicol. Extracts of Aerva lanata and Momordica charantia demonstrated antimicrobial activity on tested microorganisms. Methanolic extracts showed higher antimicrobial potential than ethyl acetate extract. Aerva lanata extracts showed better response than Momordica charantia extracts in the cup plate method antibacterial activity with Bacillus substilis and Escherichia coli.

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Phytochemical compositions and antimicrobial activity of leaf extracts of Calotropis procera against food spoilage microorganisms

AROC in Natural Products Research ◽

10.53858/arocnpr01023646 ◽

2022 ◽

Vol 1 (2) ◽

pp. 36-46

Author(s):

Toheeb D. Yissa ◽

Wahab O. Okunowo ◽

Rukayat I. Afolayan ◽

Abdulakeem R. Agboola ◽

Halima Y. Lukman ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Ethyl Acetate ◽

Inhibition Zone ◽

Calotropis Procera ◽

Phytochemical Analysis ◽

Chemical Agent ◽

Food Spoilage ◽

Leaf Extracts ◽

Spoilage Microorganisms

Background: The purpose of this study was to determine the phytochemical composition and antimicrobial potential of crude n-hexane, ethyl-acetate, methanol and aqueous extracts of Calotropis procera leaves against food spoilage microorganisms. Methods: Standard protocols were employed for the analysis of qualitative phytochemical compositions of the extracts, and antimicrobial activities against Staphylococcus aureus, Bacillus cereus, Pseudomonas aerugenosa and Aspergillus niger. Results: Phytochemical analysis revealed the presence of tannin, saponin, alkaloids, flavonoids, reducing sugar and phenolics. Terpenoids were absent in ethyl acetate and n-hexane extracts while cardiac glycoside was absent in all extracts. All extracts produced antimicrobial activity at a varying zone of inhibition. The widest inhibition zone was produced by methanol extract (21.35±0.43 mm) on staphylococcus aureus while the lowest inhibition zone (12.05±0.45 mm) was observed in the n-hexane fraction. Similarly, the widest inhibition zone (17.24±0.95 mm) was produced by methanol on A. niger while the lowest inhibition zone (5.45±0.42 mm) was produced on n-hexane on A. niger. However, the ethyl acetate extract showed no visible inhibitory zone on all the tested microorganisms. The minimum inhibitory concentration ranged from 32 mg/ml (S. aureus and B. cereus) for ethanol extracts to 128 mg/ml (B. cereus, P. aerugenosa and A. niger) for n-hexane extract. Conclusion: The result shows that the plant is a good source of bioactive compounds that can be used as a natural alternative to a chemical agent in preserving and controlling food poisoning organisms.

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Antimicrobial activity of Eurycoma longifolia and Momordica charantia

Planta Medica ◽

10.1055/s-0028-1084339 ◽

2008 ◽

Vol 74 (09) ◽

Author(s):

R Łos ◽

K Skalicka-Wozniak ◽

J Widelski ◽

A Malm ◽

K Głowniak

Keyword(s):

Antimicrobial Activity ◽

Momordica Charantia ◽

Eurycoma Longifolia

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Antimicrobial Activity of the Seed Kernel and Leaf Extracts of Mangifera indica L. (Fam. Anacardiaceae) Against Escherichia coli

Planta Medica ◽

10.1055/s-0033-1336527 ◽

2013 ◽

Vol 79 (05) ◽

Author(s):

ADC Abergas ◽

MCQ Aleria ◽

ZJS Alimagno ◽

KNC Batac ◽

AFM De Lara ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Mangifera Indica ◽

Seed Kernel ◽

Leaf Extracts

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Chemical Composition and Antimicrobial Activity of Bark and Leaf Extracts of Cupressus sempervirens and Juniperus phoenicea Grown in Al- Jabel Al-Akhdar Region, Libya

The Natural Products Journal ◽

10.2174/2210315508666180223151210 ◽

2019 ◽

Vol 9 (4) ◽

pp. 268-279

Author(s):

Mohamed E.I. Badawy ◽

Ibrahim E.A. Kherallah ◽

Ahmed S.O. Mohareb ◽

Mohamed. Z.M. Salem ◽

Hameda A. Yousef

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Sea Level ◽

Plant Extracts ◽

Complex Mixture ◽

Biologically Active ◽

Cupressus Sempervirens ◽

Leaf Extracts ◽

Juniperus Phoenicea ◽

The Impact

Background:Plant extracts are important products in the world and have been widely used for isolation of important biologically active products. Because of their significant environmental impact, extensive research has been explored to determine the antimicrobial activity of plant extracts.Methods:Acetone extracts of the bark and leaf of Cupressus sempervirens and Juniperus phoenicea, collected from three different altitudes (125, 391, and 851 m high of sea level) at Al- Jabel Al-Akhdar area, Libya were obtained and analyzed by GC/MS. The antimicrobial activity of the extracts was further evaluated against plant bacteria Rhizobium radiobacter, Erwinia carotovora, Rhodococcus fascians and Ralstonia solanacearum and fungus Botrytis cinerea.Results:The impact of the altitude from the sea level on the quantity and chemical constituents of the extracts was investigated. The yield was largely dependent on tree species and the highest yield (6.50%) was obtained with C. sempervirens L bark of altitude III (851 m of the sea level), while the lowest (1.17%) was obtained with the leaf extract of C. sempervirens L from altitude I (125 m). The chemical composition analyzed by GC/MS confirmed that the leaf extracts of C. sempervirens and J. phoenicea contained a complex mixture of monoterpene hydrocarbons, sesquiterpenes, diterpenes, diterpenoids, terpenophenolic, steroids and phthalates. However, the bark extracts of both trees contained a mixture of sesquiterpenes, diterpenes, diterpenoids, terpenophenolics, phthalates, retinol and steroids. These constituents revealed some variability among the extracts displaying the highest interesting chemotype of totarol (terpenophenolic) in all extracts (14.63-78.19% of the total extract). The extracts displayed a noteworthy antifungal potency with varying degrees of inhibition of growth with EC50 values ranged from 78.50 to 206.90 mg/L. The extracts obtained from the leaves of C. sempervirens showed that the highest inhibitory activity was obtained with the extract of altitude II (391 m) with MIC 565, 510, 380 and 710 mg/L against E. carotovora, R. fascians, and R. radiobacter and R. solanacearum, respectively.Conclusion:Based on antimicrobial activity, raw plant extracts can be a cost-effective way to protect crops from microbial pathogens. Because plant extracts contain several antimicrobial compounds, the development of resistant pathogens can be delayed.

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EFFECT OF DIFFERENT SOLVENTS ON ANTIOXIDANT ACTIVITY OF LEAF EXTRACTS OF CALOTROPIS PROCERA AND AZADIRACHTA INDICA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i1.15145 ◽

2016 ◽

Vol 10 (1) ◽

pp. 268

Author(s):

Sharmistha Banerjee ◽

Shuchi Kaushik ◽

Rajesh Singh Tomar

Keyword(s):

Antioxidant Activity ◽

Total Phenolics ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Calotropis Procera ◽

Peroxide Radical ◽

Scavenging Activity ◽

Leaf Extracts ◽

Dry Extract ◽

Leaf Powder

ABSTRACTObjective: This study was performed to identify the phytochemicals and comparatively evaluate the antioxidant activity of Calotropis procera andAzadirachta indica by detection of total phenolics, hydrogen peroxide radical scavenging activity, and estimation of condensed tannins in differentsolvent systems and at different temperatures.Methods: Leaves of C. procera and A. indica were extracted in water, methanol by soaking dried leaf powder at room temperature and also by boilingthe leaf powder in water for 30 minutes. Phytochemical tests were performed in all of the extracts. The antioxidant activity was determined byhydrogen peroxide radical scavenging activity. Quantitative estimation of total phenolics and hydrolysable tannins was also performed.Results: The total phenolics in both leaf extracts was obtained maximum in boiled extract (40.7±1.20 mg gallic acid equivalent [GAE]/g dry extractin C. procera and 33.66±1.45 mg GAE/g dry extract in A. indica). The amount of hydrolysable tannins in both leaf extracts was found to be highest inmethanol (150±1.88 mg catechin equivalent/g dry extract in C. procera and 144.8±2.63 mg catechin equivalent/g dry extract in A. indica).Conclusion: The study showed promising results indicating that these plants are a good source of antioxidants. The majority of phytochemicals wereextracted in distilled water and methanol acts as a good solvent for extraction of tannins, whereas an increase in temperature leads to poor extractionof tannins.Keywords: Antioxidant, Phytochemicals, Phenolics, Radical, Tannins.© 2017 The Authors. Published by Innovare Academic Sciences Pvt Ltd. This is an open access article under the CC BY license (http://creativecommons.

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Investigation of Antimicrobial Activity of DBD Air Plasma-Treated Banana Fabric Coated with Natural Leaf Extracts

ACS Omega ◽

10.1021/acsomega.0c02380 ◽

2020 ◽

Vol 5 (30) ◽

pp. 19034-19049

Author(s):

Mona Vajpayee ◽

Mumal Singh ◽

Lalita Ledwani ◽

Ram Prakash ◽

Sudhir Kumar Nema

Keyword(s):

Antimicrobial Activity ◽

Air Plasma ◽

Leaf Extracts ◽

Natural Leaf

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Phytochemical Screening and Antimicrobial Activity of Leaf Extracts ofAmomum nilgiricum(Thomas) (Zingiberaceae)from Western Ghats, India

Journal of Biologically Active Products from Nature ◽

10.1080/22311866.2017.1357502 ◽

2017 ◽

Vol 7 (4) ◽

pp. 311-330 ◽

Cited By ~ 1

Author(s):

Narasimha Murthy Konappa ◽

Chandra Nayaka Siddaiah ◽

Soumya Krishnamurthy ◽

Brijesh Singh ◽

Niranjana Siddapura Ramachandrappa

Keyword(s):

Antimicrobial Activity ◽

Western Ghats ◽

Phytochemical Screening ◽

Leaf Extracts

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ANTIMICROBIAL ACTIVITY OF AQUEOUS AND ETHANOLIC LEAF EXTRACTS OF ANACARDIUM OCCIDENTALE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.29073 ◽

2018 ◽

Vol 11 (12) ◽

pp. 474

Author(s):

Shobha Kl ◽

Amita Shobha Rao ◽

Pai Ksr ◽

Sujatha Bhat

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Klebsiella Pneumoniae ◽

Bacterial Infections ◽

Ethanolic Extract ◽

Culture Collection ◽

Anacardium Occidentale ◽

Diffusion Method ◽

Leaf Extracts

Objective: The objective of this study was to evaluate the antimicrobial activity of leaves of Anacardium occidentale (A. occidentale) against microorganisms including multidrug-resistant (MDR) bacteria. Methods: Agar well diffusion method was employed to demonstrate the antimicrobial activity of leaves A. occidentale. Ethanol and aqueous extracts of the leaves were used against microorganisms, which included American type culture collection strains of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, and Pseudomonas aeruginosa and the clinical isolates of Streptococcus pneumoniae, Candida albicans, MDR Escherichia coli, and MDR Klebsiella pneumoniae. Results: The ethanolic extract of leaves of A. occidentale showed significant antimicrobial activity. Aqueous extract had mild antifungal activity. Conclusion: Ethanolic extract of leaves of A. occidentale could be a good source for the antibacterials to combat MDR bacterial infections. Further studies are necessary for these potent plant extracts to evaluate the in vivo efficacy and toxicity.

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