scholarly journals Relative toxicity of aqueous leaf extracts of Lepidagathis alopecuroides (Vahl) R. Br. ex Griseb to the clariids, Clarias gariepinus and Heterobranchus bidorsalis fingerlings

2010 ◽  
Vol 1 (5) ◽  
pp. 834-840
Author(s):  
Reginlad Keremah ◽  
Irom Okey ◽  
Ugwemorubong Gabriel
Keyword(s):  
Clarias Gariepinus ◽  
Relative Toxicity ◽  
Leaf Extracts

Effects of pre-treatment with extracts of Ocimum gratissimum and Vermonia amygdalina on proximate and organoleptic properties of processed Clarias gariepinus (Burchell, 1822)

2020 ◽  
Vol 35 (1) ◽  
pp. 39-46
Author(s):  
S.A. Ojukannaiye ◽  
L.A. Agbabiaka ◽  
I.I. Adedokun
Keyword(s):  
Clarias Gariepinus ◽  
Storage Period ◽  
Live Weight ◽  
Herbal Extract ◽  
Vernonia Amygdalina ◽  
Organoleptic Properties ◽  
Leaf Extracts ◽  
Mean Values ◽  
Ocimum Gratissimum ◽  
Fish Samples

The study evaluated nutritional and organoleptic properties of Clarias gariepinus pre-treated with aqueous leave extracts of Ocimum gratissimum and Vernonia amygdalina before processing. Thirty-six (36) C. gariepinus (live weight = 1.2± 0.2kg) samples were procured and processed for the study. Fish samples were divided into three parts of twelve fish each. Each part was grouped into three treatments having four fish in each group using a completely randomized design. Treatment one (T1) had fish immersed in 5% brine without herbal extract (control). In Treatment two (T2), fish were soaked in mixture of 5% brine and 5% O. gratissium (scent leaf) extracts while Treatment three (T3) had fish soaked in mixture of 5% brine and 5% V. amygdalina (bitter leaf) extracts all for 30 minutes. Treated fish samples were smoke-dried using charcoal kiln for 5 hours at temperature of 80 – 90° C. Samples were then cooled under ambient condition (30+3oC) for 4 hours. The smoke-dried fish samples were labeled according to treatments and stored for 7 days under aseptic condition. Organoleptic properties of the samples were conducted at every 24-hour of storage using a 9-point hedonic scale. Mean values of proximate composition of samples were significantly different (p<0.05). Protein content (70.01%) in T3 was higher than 68.31% and 66.62% in T2 and T1 respectively. Similarly, fat (5.12%) and ash (4.68%) levels for T3 were higher than 4.85% and 4.46% fat with 4.36% and 3.98% ash contents for T2 and T1 respectively. Results of mean scores for each organoleptic property decreased significantly (p<0.05) during storage period with no consistent trend in score pattern. Keywords: Clarias gariepinus, extract pretreatment, Ocimum gratissimum, organoleptic, Vernonia amygdalina.

Toxicity of aquaeous leaf extracts of Gongronema latifolium on behaviourial and haematological characteristics of Clarias gariepinus (Burchell, 1822) juveniles

2020 ◽  
Vol 35 (1) ◽  
pp. 19-28
Author(s):  
J.N. Nwakpa ◽  
E.E Oti ◽  
M.U. Agim
Keyword(s):  
Blood Cell ◽  
Clarias Gariepinus ◽  
Colour Change ◽  
Threshold Value ◽  
Quality Parameters ◽  
Feed Additive ◽  
Pack Cell Volume ◽  
Leaf Extracts ◽  
Mean Values ◽  
Gongronema Latifolium

A static bioassay experiment was set up to investigate effects of acute (96-hrLC50) concentrations of aqueous leaf extracts of Gongronema latifolium on behaviourial and haematological characteristics of Clarias gariepinus (Burchell, 1822) juveniles. Evaluation of toxicity of leaf extracts at concentrations of: 0, 10, 25, 50, 75, and 100mg/l was done at various time intervals on the fish. A total of 180 catfish juveniles with average initial weight of 8.37±1.36g and total length of 12.10±2.45cm were used. The fish were distributed among the treatments on complete randomized design. Each treatment had three replicates. The results showed that aqueous leaf extracts were toxic to experimental fish. There was an observed erratic movement, colour change from black to ash-brown and dark coloured belly of treated fish. There were slight changes in water quality parameters, with no significant effect (p>0.05) on the tolerance level of fish on G. latifolium leaf extracts. Mean values of 96-hrLC50 were 90.14mg/l and 2.78mg/l for the upper and lower limits while the threshold value was 0.26mg/l. Phyto-chemical analysis of the leaves showed constituents of flavonoids, saponins, tannins, anthrocyanide, alkaloids and glycosides. Effects of aqueous extracts on haematological indices revealed increasing values of pack cell volume, haemoglobin, white blood cell, red blood cell, mean corpuscular volume, mean corpuscular heamoglobin and mean corpuscular heamoglobin concentration counts with extract concentration. Thus, G. latifolium could be used as feed additive for improved fish production at concentration below 70%. Keywords: Gongronema latifolium, leaf extracts, behaviour, haematology, Clarias gariepinus

scholarly journals Efficacy of Aloe vera Gel and Water-leaf Extracts for Removal of Egg Adhesiveness during Artificial Propagation of African Catfish (Clarias gariepinus, Burchel 1822)

2019 ◽  
pp. 1-13
Author(s):  
S. J. Fawehinmi ◽  
O. T. Adebayo ◽  
O. K. Gbadamosi
Keyword(s):  
Plant Extracts ◽  
Polynomial Regression ◽  
Clarias Gariepinus ◽  
Aloe Vera ◽  
Urea Solution ◽  
African Catfish ◽  
Leaf Extracts ◽  
Egg Hatching ◽  
Aloe Vera Gel ◽  
Immersion Period

The paper aims to study the best immersion period and concentration of the plant extracts under study (water-leaf and Aloe vera gel) that can efficiently remove egg adhesiveness of Clarias gariepinus. Data generated were subjected to Multivariate Analysis of Variance Test, Tukey multiple range tests was used as a follow up procedure. Third order Polynomial regression analysis was then used to determine the best concentration and immersion period that neutralizes adhesiveness in eggs of C. gariepinus. The experiment was carried out at The Teaching and Research Fish Farm, Federal University of Technology, Akure, between October 2018 to December 2018. One male and one female C. gariepinus brood stock weighing 1.0 kg and 1.3 kg, respectively were used for the experiment. Three different concentrations (1, 3, and 5)% of the plant extracts were used. 2 g of urea diluted in 4 g NaCl/L of water was used as reference de-adhesion agent, while water without urea solution nor plant extracts was used as control. The fish eggs were rinsed with the solutions at different durations of 1, 3 and 5 minutes. Each concentration and rinsing time was recorded in duplicates. Results of the study showed that there were no significant differences (p=0.05) in the non-adhesive egg hatching of eggs immersed in Aloe vera gel and Urea solution. Number of non-adhesive egg and hatching increased in waterleaf extract which was significantly different (P=0.05) from eggs immersed in Aloe vera gel and water but not significantly different from urea solution. Therefore this study shows that waterleaf extract at 1% concentration and 1 minute immersion period can efficiently remove egg adhesiveness and increase hatching in C. gariepinus.

scholarly journals Toxic Potential and Metabolic Profiling of Two Australian Biotypes of the Invasive Plant Parthenium Weed (Parthenium hysterophorus L.)

Toxins ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 447
Author(s):  
Ali Ahsan Bajwa ◽  
Paul A. Weston ◽  
Saliya Gurusinghe ◽  
Sajid Latif ◽  
Steve W. Adkins ◽  
...  
Keyword(s):  
Invasive Plant ◽  
Leaf Tissue ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Invasion Success ◽  
Parthenium Hysterophorus ◽  
Relative Toxicity ◽  
Leaf Extracts ◽  
Secondary Chemistry ◽  
Parthenium Weed

Parthenium weed (Parthenium hysterophorus L.) is an invasive plant species in around 50 countries and a ‘Weed of National Significance’ in Australia. This study investigated the relative toxicity of the leaf, shoot and root extracts of two geographically separate and morphologically distinct biotypes of parthenium weed in Queensland, Australia. Parthenium weed exhibited higher phytotoxic, cytotoxic and photocytotoxic activity in leaf tissue extracts in contrast to shoot and root. The germination and seedling growth of a dicot species (garden cress) were inhibited more than those of a monocot species (annual ryegrass) using a phytotoxicity bioassay. The cytotoxicity of leaf extracts was assessed in a mouse fibroblast cell suspension assay and increased under high ultraviolet A(UV-A) radiation. A major secondary metabolite, parthenin, was found in abundance in leaf extracts and was positively correlated with cytotoxicity but not with photocytotoxicity or phytotoxicity. Ambrosin and chlorogenic acid were also detected and were positively correlated with germination inhibition and the inhibition of radicle elongation, respectively. In addition, other currently unidentified compounds in the leaf extracts were positively correlated with phytotoxicity, cytotoxicity and photocytotoxicity with two to three molecules strongly correlated in each case. Both parthenium weed biotypes investigated did not differ with respect to their relative toxicity, despite their reported differences in invasive potential in the field. This suggests that secondary chemistry plays a limited role in their invasion success.

scholarly journals The resistance of sangkuriang catfish (Clarias gariepinus Burchell 1822) against Aeromonas hydrophila bacteria given moringa leaf extracts (Moringa oleifera L.) through the feed

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
. Rosidah ◽  
Ibnu Dwi Buwono ◽  
Walim Lili ◽  
Ibnu Bangkit Suryadi ◽  
Ade Reza Triandika
Keyword(s):  
Survival Rate ◽  
Clinical Sign ◽  
Aeromonas Hydrophila ◽  
Leaf Extract ◽  
Leukocyte Count ◽  
Clarias Gariepinus ◽  
Total Leukocyte Count ◽  
Leaf Extracts ◽  
Significance Level ◽  
Moringa Leaf Extract

This study aims to reveal effective concentration of moringa leaf extract which is inducing body resistance of sangkuriang catfish against Aeromonas hydrophila. This study used as experimental method of Completely Randomized Design (CRD) with six treatments and three replications. The treatment used in this research are the addition of Moringa leaf extract into feed with concentration of 0 ppm (A), 100 ppm (B), 125 ppm (C), 150 ppm (D), 175 ppm (E) and 200 ppm (F). Observed Parameters are total leukocyte count, increase in leukocyte, clinical sign and survival rate.  Increase in leukocyte and survival rate data were analyzed using F test and Duncan test with significance level 0,05, while clinical sign data was analyzed descriptively. The results showed that Moringa leaf extract was effective to induce the immune of sangkuriang catfish. The 150 ppm concentration resulted the highest total leukocyte count of 23.46 ± 6.46% and didn’t have clinical symptons after challenge with Aeromonas hydrophila and highest survival rate 80.0 ± 5.00%. Based on the regression analysis, 153.95 ppm of Moringa leaf extract is the optimum concentration with 95.62% on the survival of sangkuriang catfish after challenge with Aeromonas hydrophila.AbstrakPenelitian ini bertujuan untuk menentukan konsentrasi yang efektif dari ekstrak daun kelor untuk menginduksi ketahanan tubuh ikan lele sangkuriang terhadap serangan bakteri Aeromonas hydrophila. Penelitian ini menggunakan metode eksperimental Rancangan Acak Lengkap dengan enam perlakuan dan tiga ulangan. Perlakuan penelitian yang digunakan adalah penambahan ekstrak daun kelor ke dalam pakan dengan konsentrasi 0 ppm (A), 100 ppm (B), 125 ppm (C), 150 ppm (D), 175 ppm (E) dan 200 ppm (F). Parameter yang diamati meliputi jumlah total sel darah, peningkatan jumlah sel darah putih, gejala klinis, dan sintasan. Data peningkatan jumlah sel darah putih dan sintasan dianalisis menggunakan uji F dan uji jarak berganda pada taraf kepercayaan 5%, sedangkan data gejala klinis dianalisis secara deskriptif. Hasil penelitian menunjukkan ekstrak daun kelor efektif untuk menginduksi ketahanan tubuh benih lele sangkuriang. Konsentrasi 150 ppm menghasilkan rata-rata jumlah sel darah putih tertinggi 23,46±6,46% dan setelah diuji tantang tidak menunjukkan gejala klinis, serta tingkat sintasan yang tertinggi sebesar 80,0±5,00%. Berdasarkan analisis regresi konsentrasi ekstrak daun kelor memberikan pengaruh sebesar 95,62% dengan konsentrasi optimum sebesar 153,95 ppm terhadap sintasan ikan lele sangkuriang setelah di uji tantang dengan Aeromonas hydrophila.

scholarly journals Behavioural Responses and Mortality of Clarias gariepinus Juveniles Exposed to Acute Concentrations of Paraquat

2021 ◽  
pp. 49-54
Author(s):  
Okey I. B. ◽  
Ayotunde E. O. ◽  
Patrick B. U.
Keyword(s):  
Clarias Gariepinus ◽  
Relative Toxicity ◽  
Lethal Effects ◽  
Cumulative Mortality ◽  
Behavioural Responses ◽  
Behavioural Changes ◽  
Aquatic Pollutants ◽  
Loss Of Balance ◽  
Toxicity Factor ◽  
Lethal Time

Paraquat is the most common contact and non- selective herbicide for exterminating vegetative pest. Fish are ideal sentinels for detecting aquatic pollutants and are largely used as bio indicators of environmental pollution. This study is aimed to determine the behavioural changes, lethal concentrations (LCs) and mean lethal time (MLT) of paraquat exposed to Clarias gariepinus. A 96 hours renewable bioassay was conducted with various paraquat concentrations 0.0, 0.25, 0.50, 0.75, 1.0 and 1.25 mg/l. Behavioural changes and cumulative mortality were observed and recorded at 12, 24, 48, 72 and 96 hour. Behavioural changes such as air gulping, erratic swimming, loss of balance, excessive mucus secretion, discolouration and death were observed with severity increasing as concentration and duration of exposure increases. The LC50 values were decreased from 0.191mg/l (0.171 – 0.222) in 12 hour to 0.107mg/l (0.065 – 0.150) in 96 hour, while relative toxicity factor (TF) was increased from 1 to 1.79 times respectively. The 96hr MLT values were decreased from 91.18 hours (54.09-105.64) at the lowest concentration to 16.22 hours (9.06 – 25.15) at the highest concentration with relative toxicity time (RTT) increasing from 1 to 5.62 times. Herbicide should be apply with caution and studies on the sub lethal effects of paraquat on the haematological, biochemical and histological parameters of C. gariepinus juveniles will be necessary.

scholarly journals Responses of two plant-derived bioinsecticides as protectants of smoke-dried catfish, Clarias gariepinus [Pisces: Clariidae] against hide beetle, Dermestes maculatus (De Geer) [Coleoptera: Dermestidae]

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Kayode David Ileke
Keyword(s):  
Clarias Gariepinus ◽  
Adult Mortality ◽  
Tithonia Diversifolia ◽  
Vernonia Amygdalina ◽  
Leaf Extracts ◽  
Plastic Container ◽  
Smoked Fish ◽  
Dermestes Maculatus ◽  
Significant Difference ◽  
And Control

Abstract Background Toxicities of Vernonia amygdalina and Tithonia diversifolia leaf powders and extracts on larva and adult mortality of Dermestes maculatus on smoke-dried catfish were evaluated in the laboratory. The leaf powders were admixed at 1, 3, 6, 9 and 12 g/100 g of smoked catfish in 500 ml plastic container while plant extracts were tested at 1, 3, 6, 9 and 12% concentrations. Results The toxicities of the plant products were concentrations and exposure time dependent. Significant difference (P < 0.05) existed between the toxicity of V. amygdalina and T. diversifolia leaf powders and control. Tithonia diversifolia powder evoked 27.5, 40, 52.7, 60 and 82.5% larval mortalities at the various concentrations after 24 h of exposure of the catfishes to the plant powder. Similarly, V. amygdalina leaf powders caused 20, 30, 42.5, 52.5 and 77.5% larval mortalities at varying concentrations of 24 h intervals of exposure to the plant powder. Tithonia diversifolia powder achieved 100% mortality at 9 g dosage after 96 h of treatment. Similar trend was achieved on the response of both plant leaf extracts as protectants of smoke-dried catfish against hide beetle. Conclusion Tithonia diversifolia powder and extract were more lethal than bitter leaf (V. amygdalina) and could be integrated as smoked fish protectant against hide beetle (D. maculatus).

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