Albumin nanoparticles Preparation, Characterization and In-Vitro Safety Evaluation

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
dina youssef ◽  
fatma sallam ◽  
Taher Salaheldin ◽  
Samah Darwish ◽  
Abeer El-Metwally ◽  
...  
Keyword(s):  
Safety Evaluation ◽  
Albumin Nanoparticles

scholarly journals Albumin nanoparticles Preparation, Characterization and In-Vitro Safety Evaluation

2021 ◽  
Vol 38 (2) ◽  
pp. 183-196
Author(s):  
dina youssef ◽  
Fatma A. Sallam ◽  
Taher A. Salaheldin ◽  
Samah F. Darwish ◽  
Abeer E. El-Metwally ◽  
...  
Keyword(s):  
Safety Evaluation ◽  
Albumin Nanoparticles

Ganciclovir-loaded albumin nanoparticles: characterization and in vitro release properties

2001 ◽  
Vol 12 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Marta Merodio ◽  
Amaia Arnedo ◽  
M.Jesús Renedo ◽  
Juan M Irache
Keyword(s):  
In Vitro Release ◽  
Albumin Nanoparticles ◽  
Nanoparticles Characterization ◽  
Vitro Release

Anticancer activity in vitro and biological safety evaluation in vivo of Sika deer antler protein

2017 ◽  
Vol 41 (6) ◽  
pp. e12421 ◽  
Author(s):  
Huihai Yang ◽  
Lulu Wang ◽  
Hang Sun ◽  
Xiaofeng He ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Sika Deer ◽  
Safety Evaluation ◽  
Deer Antler ◽  
Biological Safety

scholarly journals Biological Safety Evaluation of 99mTc-DTPA-Ketoconazole for Diagnosis of Fungal Infection

2017 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Maula Eka Sriyani ◽  
Hendris Wongso ◽  
Eva Maria Widyasari ◽  
Rizky Juwita Sugiharti ◽  
Iim Halimah ◽  
...  
Keyword(s):  
Single Dose ◽  
Fungal Infection ◽  
Radiochemical Purity ◽  
Safety Evaluation ◽  
Fungal Growth ◽  
In Vitro Assays ◽  
Toxic Response ◽  
Biological Safety ◽  
Safety Parameters

Infectious diseases have become one of the leading cause of mortality around the world, including in the Southeast Asia. One of the microbial that cause infection is fungi. Occasionally, deep-seated fungal infection is difficult to detect using conventional diagnosis methods and therefore leads to inaccurate detection. Our previous research was conducted in order to obtain the labeled compound of <sup>99m</sup>Tc-DTPA-Ketoconazole with a high radiochemical purity (98.40 ± 0.86%). Moreover, the in-vitro assays showed that <sup>99m</sup>Tc-DTPA-Ketoconazole can potentially bind to Candida albicans. On the other hand, in clinical routine use, diagnostic kit should be safe for the patients. Consequently, this research was conducted to determine the biological safety parameters of <sup>99m</sup>Tc-DTPA-Ketoconazole on the animal study, including single dose and acute toxicity test, sterility, and apirogenicity test. The results showed that both the single dose at 34.6 μCi and dose until 149 times of the single dose did not stimulate the toxic response to the animals. In addition, the sterility data revealed that there was no microbial growth after 7 days of incubation at 37°C as well as fungal growth after 14 days of incubation at 25°C. Furthermore, the apirogenicity test using rabbits revealed that there was no increase in temperature more than 0.6°C for each animal and not more than 1.5°C of total increase of temperature for all the animals. It is concluded that the <sup>99m</sup>Tc-DTPA-Ketoconazole is satisfy the requirements of biological safety of a radiopharmaceutical and therefore was acceptable for fungal detection in nuclear medicine.

Elaboration of curcumin-loaded rice bran albumin nanoparticles formulation with increased in vitro bioactivity and in vivo bioavailability

2018 ◽  
Vol 77 ◽  
pp. 834-842 ◽  
Author(s):  
Chun Liu ◽  
Xiaoquan Yang ◽  
Wei Wu ◽  
Zhao Long ◽  
Huaxi Xiao ◽  
...  
Keyword(s):  
Rice Bran ◽  
In Vitro Bioactivity ◽  
Albumin Nanoparticles ◽  
Rice Bran Albumin

Development of an in vitro screening method for safety evaluation of nanomaterials

2009 ◽  
Vol 19 (1) ◽  
pp. 19-27 ◽  
Author(s):  
Atsuko Matsuoka ◽  
Agneta Önfelt ◽  
Yoshie Matsuda ◽  
Ryusuke Nakaoka ◽  
Yuji Haishima ◽  
...  
Keyword(s):  
Screening Method ◽  
Safety Evaluation ◽  
In Vitro Screening

Evaluation of low molecular weight polyethylenimine-introduced chitosan for gene delivery to mesenchymal stem cells

2020 ◽  
Vol 10 (7) ◽  
pp. 1170-1176
Author(s):  
Minchen Liu ◽  
Yulan Hu ◽  
Yi Feng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
A549 Cells ◽  
Safety Evaluation ◽  
Physicochemical Characteristics ◽  
Hatching Rate

This study aimed to examine the transfection ability of polyethylenimine (PEI) (1800 Da)-grafted chitosan (10 kDa) (CP), a newly synthesized PEI derivative, in mesenchymal stem cells (MSCs). The safety evaluation of the complex/DNA was studied in vitro and in vivo. In addition, CP/pGL3 was applied to investigate the effects of transfection efficiency. In this study, CP/DNA can be formed with compatible physicochemical characteristics for gene delivery. CP cytotoxicity decreased in A549 cells. Moreover, a zebrafish embryo model was used for evaluating the safety in vivo. Compared to the PEI (25 kDa) group, the zebrafish hatching rate increased and the mortality rate decreased in the CP/DNA group, which provided an indication of the safety of CP. In comparison with chitosan (100 kDa)-PEI (1200 Da), CP's transfection efficiency was higher in both A549 cells and MSCs. This study aimed to lay the foundation for further applications of CP in gene delivery. Therefore, further gene therapy investigations of CP by using MSCs need to be performed.

Chemical characterisation, in vitro antioxidant, cytotoxicity and safety evaluation of Polystichum braunii (Spenn.) fee roots

2020 ◽  
pp. 1-6
Author(s):  
Ammara Saleem ◽  
Mohammad Saleem ◽  
Muhammad Furqan Akhtar ◽  
Azhar Rasul ◽  
Mirza Muhammad Faran Ashraf Baig
Keyword(s):  
Safety Evaluation

scholarly journals Safety evaluation of resveratrol as an active compound for drug-eluting cardiovascular implants

2019 ◽  
Vol 5 (1) ◽  
pp. 331-333
Author(s):  
Valeria Khaimov ◽  
Thomas Reske ◽  
Claudia Matschegewski ◽  
Niels Grabow ◽  
Thomas Eickner
Keyword(s):  
Prolonged Exposure ◽  
Animal Experiments ◽  
Safety Evaluation ◽  
Local Drug Delivery ◽  
Surrounding Tissue ◽  
Interesting Candidate ◽  
Drug Eluting ◽  
Cardiovascular Implants ◽  
Animal Tests

AbstractResveratrol is a member of stilbenoids with promising anti-atherosclerotic properties. This hallmark makes it an extremely interesting candidate for local drug delivery to damaged tissue adjacent to the implant in order to reduce implant-related complications. For the regulatory approval drug-eluting medical devices have to be thoroughly tested for safety, efficacy and interactions with the surrounding tissue, including tests for sensitization among others. Studies for sensitization help to estimate the risk for an allergic reaction upon prolonged exposure to a chemical compound. Due to increased social and regulatory demand for replacement of animal experiments by in vitro approaches a number of reliable predictive non-animal tests have been developed. Here, we assessed the skin sensitization potential of resveratrol by the direct peptide reactivity assay (DPRA), one of the first non-animal tests adopted by the OECD.

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