scholarly journals Yeast isolation and identification in water used in a Brazilian hemodialysis unit by classic microbiological techniques and Raman spectroscopy

2017 ◽  
Vol 16 (2) ◽  
pp. 311-320 ◽  
Author(s):  
Lilian Bueno Montanari ◽  
Flávio Garcia Sartori ◽  
Diórginis Bueno Montrazi Ribeiro ◽  
Luís Fernando Leandro ◽  
Regina Helena Pires ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Yeast Species ◽  
Water Disinfection ◽  
Isolation And Identification ◽  
Minimal Inhibitory Concentrations ◽  
Hemodialysis Unit ◽  
Microbiological Techniques ◽  
Yeast Isolation ◽  
Excellent Tool ◽  
Effective Water

Abstract The use of poorly treated water in hemodialysis centers may lead to fungal contamination, which poses a serious threat to immunologically debilitated hemodialysis patients. This study aimed to isolate and identify yeast species in the water of a Brazilian hemodialysis center by using classic microbiological techniques and Raman spectroscopy. For 12 months, a total of 288 water samples were collected from different points of the hemodialysis treatment distribution center. One hundred and forty-six yeast species were isolated and identified in the samples that tested positive for the presence of yeasts such as Candida parapsilosis (100 isolates, or 68.50%), C. guilliermondii (17 isolates, or 11.65%), Rhodotorula mucilaginosa (23 isolates, or 15.75%), R. glutinis (three isolates, or 2.05%), and Trichosporon inkin (three isolates, or 2.05%). Yeast susceptibility to the antifungal fluconazole was also assayed. Only two C. guilliermondii isolates were resistant to fluconazole: the minimal inhibitory concentrations were higher than 64 μg/mL. The different yeast species present in the water of a Brazilian hemodialysis center call for more effective water disinfection procedures in this unit. Raman spectroscopy is an excellent tool to identify yeast species and is potentially applicable in routine water monitoring in hemodialysis units.

scholarly journals Yeast isolation and identification during on-farm cocoa natural fermentation in a highly producer region in northern Brazil

2021 ◽  
Vol 16 (12) ◽  
Author(s):  
Gilson Celso Albuquerque Chagas Junior ◽  
Julio Cézar Araújo do Espírito-Santo ◽  
Nelson Rosa Ferreira ◽  
Silvia Helena Marques-da-Silva ◽  
Guilherme Oliveira ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Natural Fermentation ◽  
Northern Brazil ◽  
On Farm ◽  
Yeast Isolation

scholarly journals Effective water disinfection using magnetic barium phosphate nanoflakes loaded with Ag nanoparticles

2019 ◽  
Vol 218 ◽  
pp. 173-182 ◽  
Author(s):  
Fan Zhang ◽  
Meng Hao Lee ◽  
Yuxiong Huang ◽  
Arturo A. Keller ◽  
Sanghamitra Majumdar ◽  
...  
Keyword(s):  
Ag Nanoparticles ◽  
Water Disinfection ◽  
Effective Water

Hollow Fiber Membrane Decorated with Ag/MWNTs: Toward Effective Water Disinfection and Biofouling Control

ACS Nano ◽  
2011 ◽  
Vol 5 (12) ◽  
pp. 10033-10040 ◽  
Author(s):  
Poernomo Gunawan ◽  
Cong Guan ◽  
Xianghua Song ◽  
Quanyuan Zhang ◽  
Susanna Su Jan Leong ◽  
...  
Keyword(s):  
Hollow Fiber ◽  
Hollow Fiber Membrane ◽  
Water Disinfection ◽  
Fiber Membrane ◽  
Biofouling Control ◽  
Effective Water

Development of silver-containing nanocellulosics for effective water disinfection

Cellulose ◽  
2014 ◽  
Vol 21 (3) ◽  
pp. 1965-1974 ◽  
Author(s):  
M. Gouda ◽  
A. A. Hebeish ◽  
M. A. Al-Omair
Keyword(s):  
Water Disinfection ◽  
Effective Water

scholarly journals Identifying the Diversity of Dominant LABs from Fermented Dairy Products Dahi and Yoghurt in Eastern Region of Nepal

2019 ◽  
Vol 11 ◽  
pp. 60-64
Author(s):  
Sanjay Mahato ◽  
Aakash Kumar Shahani
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Bulgaricus ◽  
Eastern Region ◽  
Isolation And Identification ◽  
Carbohydrate Utilization ◽  
Variable Nature ◽  
Microbiological Techniques ◽  
Fermented Dairy

The aim of this study was to isolate and identify lactic acid bacteria diversity from dahi/ yoghurt. Curd, also known as commercially prepared yoghurt or homemade Dahi, is formed during the slow lactic fermentation of lactose from milk by thermophilic lactic acid bacteria (LAB). Thirty-five samples of yoghurt and curd were collected from the Biratnagar and isolation and identification of bacteria were done by various microbiological techniques like MRS Agar inoculation, colony characteristics, microscopic and biochemical examination. A total of sixty-six strains of lactobacilli were isolated from curd and identification of strains was done by biochemical and carbohydrate utilization test. Lactobacillus bulgaricus, L. casei, L. fermentum, L. acidophilus, and Streptococcus thermophilus were identified from curd. pH of samples was between 3.0 to 4.0 for homemade dahi; while 4.34 to 4.5 for commercially available yoghurt. The mean colony count of lactic acid bacteria was 1.4x107–4.9x107 cfu/g. 37.9% of samples contained Streptococcus thermophilus, 30.3% had Lactobacillus bulgaricus. Forty-five isolates from 24 industrial yoghurt samples showed 37.5% of the yoghurt contained both Lactobacillus bulgaricus and Streptococcus thermophilus followed by 25% samples having S. thermophilus and L. acidophilus. Other species like L. fermentum and L. casei were less common. From 11 homemade dahi samples, 54.5% of curd possessed both S. thermophilus and L. bulgaricus; 18.2% curd had both S. thermophilus and L. fermentum. The study concludes that L. bulgaricus and Streptococcus thermophilus are prevalent potent lactic acid bacteria. This study provides an account of the diversity of lactic acid bacteria in dahi/ yoghurt which will provide useful information about the variable nature of curd in this region to future researchers.

scholarly journals Isolation and identification of Enteropathogenic Escherichia coli (EPEC) in paediatric diarrhoeal patients by detection of bfpA gene by PCR

2013 ◽  
Vol 7 (2) ◽  
pp. 02-05
Author(s):  
Shimu Saha ◽  
Sanya Tahmina Jhora ◽  
Shikha Paul ◽  
Israt Jahan Azmi ◽  
Tarek Mahbub Khan
Keyword(s):  
Polymerase Chain Reaction ◽  
Tertiary Care ◽  
Diarrhoeal Disease ◽  
Care Hospital ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Two Samples ◽  
Microbiological Techniques ◽  
Polymerase Chain

This study has been undertaken to investigate the isolation and identification of EPEC strains from paediatric diarrhoeal patients.Total 300 samples were studied.Two hundred and seventy two samples from patients with diarrhoea and  28 samples from control children were collected from two tertiary care hospital. Esch. coli was isolated and identified from all the 300 samples including patient and control using standard microbiological techniques. EPEC strains were identified on the basis of  presence of bundle forming pilus (bfpA) gene. Out of 272 samples from diarrhoeal patient only Esch. coli was isolated from 240 (88.2%) samples. Shigella spp. with Esch. coli were isolated from 27 (10%) specimens and Salmonella spp. with Esch. coli were isolated from 5 (1.8%) samples. Among 272 samples 20 (7.35%) isolates were identified as EPEC on the basis of presence of bfpA gene detected by polymerase chain reaction. EPEC strains were identified from those 240 samples, from which Esch. coli had been isolated only. No EPEC strain was identified  from control children. Rapid and reliable detection of EPEC is required for successful microbiological surveillance and for treatment of EPEC mediated diarrhoeal disease. bfpA gene detection by polymerase chain reaction can be a appropriate method where facilities for polymerase chain reaction are available.DOI: http://dx.doi.org/10.3329/bjmm.v7i2.19324 Bangladesh J Med Microbiol 2013; 07(02): 2-5

scholarly journals Using Wood’s glass to enhance the efficiency of a water solar disinfection (SODIS) apparatus with a Fresnel lens

2020 ◽  
Author(s):  
Ammar Alkhalidi ◽  
Sameer Arabasi ◽  
Abeer Abu Othman ◽  
Tareq Sabanikh ◽  
Linda Mahmood ◽  
...  
Keyword(s):  
Solar Radiation ◽  
Ultraviolet Light ◽  
Fresnel Lens ◽  
Water Disinfection ◽  
Band Pass Filter ◽  
Low Carbon ◽  
Pass Filter ◽  
Solar Disinfection ◽  
Disinfection Process ◽  
Effective Water

Abstract Solar disinfection, as a low carbon technology, is a sustainable and cost-effective water treatment method, which makes it more likely to be implemented in developing countries. It has been proven that ultraviolet light radiation is effective against pathogens and microorganisms. The aim of this paper is to evaluate the performance of solar disinfection apparatus that is equipped with Wood’s glass and a Fresnel lens. Wood’s glass is a band-pass filter that allows ultraviolet light to go through and blocks most of the remaining spectrum including infrared. This serves a dual purpose. It lets through the part that inactivates pathogens and microorganisms (ultraviolet) and blocks the part that helps the growth of bacteria (infrared). Experimental setup is designed to investigate the effect of Wood’s glass with and without a Fresnel lens on the solar disinfection process. The investigation is done on a batch-based system and on a novel instantaneous water disinfection system. Results of water disinfection process were recorded for each set of experiments taking into considerations vital parameters, such as solar radiation, solar ultraviolet light intensity and water temperature. The results of these experiments showed effective water disinfection up to 85% pathogens removal by the use of daily available solar radiation.

scholarly journals Bacteriological and Parasitological Assessment of Water use in Selected Abattoirs in Port Harcourt Metropolis, Rivers State, Nigeria

2021 ◽  
pp. 19-25
Author(s):  
Eze Chinwe Nwadiuto ◽  
Ihua Nnenna ◽  
Ijewere Blessing Ofureb
Keyword(s):  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Cross Sectional Study ◽  
Coliform Bacteria ◽  
Isolation And Identification ◽  
Surrounding Water ◽  
Cross Sectional ◽  
Port Harcourt ◽  
Microbiological Techniques ◽  
Rivers State

In the abattoir, large amount of water is being used in processing operations; and this produces huge amount of wastewater which empty into the surrounding water bodies. The cross-sectional study followed the conventional microbiological techniques of macroscopy, microscopy, isolation, and identification to assess the bacteriological and parasitological examination of water sources in selected abattoirs in Port Harcourt Metropolis, Rivers State, Nigeria. The result of microbial population showed Total Coliform ranging from 5 x 101cfu/ml – 2.80 x 102cfu/ml and Faecal Coliform bacteria of 2.80 x 101 cfu/ml -1.85 x 102 cfu/ml. Total Heterotrophic bacteria ranged from 7.8 x101cfu/ml to 4.2 x102cfu/ml. The mean microbial counts; 4.86 x101 cfu/ml Fecal Coliform, 9.65 x 101cfu/ml Total Coliform and 1.82 x 102cfu/ml Heterotrophic Count. The isolates identified were Klebsiella spp, Shigella spp., Salmonella typhymurium, E. coli, Proteus spp., Salmonella paratyphi, Vibrio cholera. Nkpor village abattoir recorded highest occurrence of bacteria isolate with a total of 50(22.03%) followed by Iwofe 48(21.15%). Water sample from ponds had a higher parasitic contamination while samples from the borehole were free from parasitic infestation. Water samples for parasitological assessment were filtered and examined for parasitic organism using standard methods. Four different parasites were identified in the study, they include Ascaris lumbricoides (32.5%), Entamoeba histolytica (22.5%), Giardia lamblia (17.5%) and the least Taenia spp (7.5%). Nkpor-village abattoir had the highest level of contamination with (30.0%) parasite occurrence, followed by Mgboshimini abattoir (15.0%). The use of unsafe water for meat processing by butchers, portends a serious public health risk to consumers who purchase the meat, from these abattoirs. Therefore, government should endeavour to make borehole water available to all the abattoirs in Port Harcourt.

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