Prevalence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in wastewater: a systematic review and meta-analysis

Abstract Wastewater is considered a hotspot niche of multi-drug and pathogenic bacteria such as Enterobacteriaceae-producing extended-spectrum beta-lactamases (ESBL-E). Thus, the aim of this meta-analysis was to evaluate the prevalence of ESBL-E in different wastewater sources. Different databases (Medline, EMBASE, and Cochrane Library) were searched from inception to March 2021. Data were analyzed using random-effects modeling, and subgroup and meta-regression analyses were used to ascertain heterogeneity among the subgroups. Fifty-seven observational studies were selected, and the pooled prevalence of ESBL-E in wastewater was 24.81% (95% CI, 19.28–30.77). Escherichia coli had the highest ESBL prevalence. The blaCTX-M genes were the most prevalent in the selected studies (66.56%). The pooled prevalence of ESBL was significantly higher in reports from America (39.91%, 95% CI, 21.82–59.51) and reports studying hospital and untreated wastewaters (33.98%, 95% CI, 23.82–44.91 and 27.36%, 95% CI, 19.12–36.42). Overall, this meta-analysis showed that the prevalence of ESBL-E in wastewater is increasing over time and that hospital wastewater is the most important repository of ESBL-E. Therefore, there is a need for developing new sewage treatment systems that decrease the introduction of resistant bacteria and antibiotic residues.

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Prevalence of Extended-Spectrum Beta-Lactamases-Producing Microorganisms in Patients Admitted at KRRH, Southwestern Uganda

International Journal of Microbiology ◽

10.1155/2017/3183076 ◽

2017 ◽

Vol 2017 ◽

pp. 1-5 ◽

Cited By ~ 8

Author(s):

Baguma Andrew ◽

Atek Kagirita ◽

Joel Bazira

Keyword(s):

Healthcare Workers ◽

Pathogenic Bacteria ◽

Regional Referral Hospital ◽

Beta Lactamases ◽

Extended Spectrum Beta Lactamase ◽

E Coli ◽

Extended Spectrum ◽

Diffusion Technique ◽

Extended Spectrum Beta Lactamases ◽

Southwestern Uganda

The emergence of extended-spectrum beta-lactamase- (ESBL-) producing pathogenic bacteria at Kabale Regional Referral Hospital (KRRH), located in southwestern Uganda, is of great concern: a phenomenon that worries clinicians and other healthcare workers due to the serious threat they pose to patients. This current study aimed at determining the phenotypic detection of ESBL-producing strains ofE. coli, Klebsiellasp., andProteussp. isolated from clinical specimens and their prevalence in patients admitted at KRRH. We used combined disc diffusion technique to detect and establish the presence of ESBLs-producing bacteria. Of the 100 tested bacterial isolates, 89 (89%) were identified as ESBL-producing bacteria.Klebsiellasp. predominated in the samples (46 (52%)), presenting the highest frequency of ESBLs producing followed byE. coli(39 (44%)) andProteus mirabilis(4 (4.5%)) from the combined disk diffusion.

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The magnitude of extended-spectrum beta-lactamase- producing Enterobacteriaceae from clinical samples in Ethiopia: a systematic review and meta-analysis

Access Microbiology ◽

10.1099/acmi.0.000195 ◽

2021 ◽

Author(s):

Kuma Diriba ◽

Ephrem Awulachew ◽

Aschelew Gemede ◽

Asrat Anja

Keyword(s):

Systematic Review ◽

Type Species ◽

Meta Analysis ◽

High Rate ◽

Cochrane Library ◽

Clinical Samples ◽

Content Type ◽

Link Type ◽

Extended Spectrum ◽

Pooled Prevalence

Background. The rapid spread of resistance among extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is a serious problem around the world. It results in serious clinical complications in humans and has become a global threat. Therefore, this systematic review and meta-analysis was aimed to estimate the pooled prevalence of ESBL-producing Enterobacteriaceae in different clinical samples in Ethiopia. Methods. A systematic search was conducted on PubMed, Web of Science, Embase, Google Scholar and the Cochrane Library. All identified observational studies reporting the prevalence of ESBL-producing Enterobacteriaceae from clinical samples in Ethiopia were included. Four authors independently extracted data and analysed using R software version 3.6.1 and STATA statistical software version 13. A random-effects model was computed to estimate the pooled prevalence of ESBL-producing Enterobacteriaceae in Ethiopia. Results. Of 142 articles reviewed, 14 studies that fulfilled the inclusion criteria were included in the meta-analysis. The pooled prevalence of ESBL-producing Enterobacteriaceae in the different clinical specimens in Ethiopia was 49 % (95 % CI: 39, 60). Klebsiella pneumoniae was the leading ESBL-producing Enterobacteriaceae followed by Escherichia coli and Acinetobacter baumannii with a prevalence of 74, 67 and 60 %, respectively. ESBL-producing isolates showed a high rate of resistance to cefotaxime, ceftriaxone, ceftazidime, Amoxicillin clavulanic acid (AMC), ampicillin and aztreonam. The better options for the treatment of ESBL-producing Enterobacteriaceae are amikacin and Imipenem. Conclusion. The magnitude of ESBL-producing Enterobacteriaceae in different clinical samples in Ethiopia is alarmingly high and represents a threat to human health. Hence, a coordinated effort needs to be implemented for the prevention and control of these Enterobacteriaceae .

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The effect of Enterococcus faecium M74 feed additive on the extended-spectrum beta-lactamases/AmpC-positive Escherichia coli faecal counts in pre-weaned dairy calves

Acta Veterinaria Brno ◽

10.2754/avb201786040333 ◽

2017 ◽

Vol 86 (4) ◽

pp. 333-338 ◽

Cited By ~ 2

Author(s):

Jana Šmídková ◽

Alois Čížek

Keyword(s):

Escherichia Coli ◽

Enterococcus Faecium ◽

Dairy Calves ◽

Resistant Bacteria ◽

Beta Lactamases ◽

New Born ◽

Extended Spectrum ◽

Faecal Samples ◽

Probiotic Treatment ◽

Extended Spectrum Beta Lactamases

The increasing occurrence of extended-spectrum beta-lactamases and/or AmpC-positive Escherichia coli among different species of food producing animals poses a threat to public health. The animal gut plays a key role in the development of antibiotic resistant bacteria, allowing the selection, multiplication and subsequent contamination of the farm environment. However, applicable procedures for reducing such bacteria on farms are currently unavailable. The present study was aimed to determine whether a probiotic administration to new-born dairy calves would reduce faecal shedding of extended-spectrum beta-lactamases and/or AmpC-positive Escherichia coli during the pre-weaning period. Ten randomly assigned new-born Holstein calves on a dairy farm with recent evidence of high occurrence of AmpC-positive Escherichia coli among calves were treated by a probiotic mix within 12 h after birth. Nine control calves were not treated. Faecal samples were collected from each calf daily on days 2 through 5, and then on days 7, 10, and 14. The faecal samples were cultured, and the mean numbers of cefotaxime-resistant Escherichia coli and confirmed enteroaggregative Escherichia coli were compared between the two groups. Results suggested that the Enterococcus faecium probiotic treatment (Enterococcus faecium M74, NCIMB 11181) of new-born calves did not reduce the enteroaggregative Escherichia coli counts in their faeces. There was no significant difference in the shedding of enteroaggregative Escherichia coli between the probiotic-treated and control calves throughout the two-week study period.

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Prevalence of Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Ethiopia: A Systematic Review and Meta-Analysis

International Journal of Microbiology ◽

10.1155/2021/6669778 ◽

2021 ◽

Vol 2021 ◽

pp. 1-13

Author(s):

Teklehaimanot Kiros ◽

Lema Workineh ◽

Tegenaw Tiruneh ◽

Tahir Eyayu ◽

Shewaneh Damtie ◽

...

Keyword(s):

Systematic Review ◽

Outcome Measures ◽

Literature Search ◽

Meta Analysis ◽

Health Concern ◽

Cochrane Library ◽

Clinical Samples ◽

Published Data ◽

Extended Spectrum ◽

Pooled Prevalence

Background. Antimicrobial resistance especially caused by extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) has become a global public health concern. Globally, these isolates have remained the most important causes of several infections and associated mortality. Their rapid spread in Ethiopia is associated with a lack of regular surveillance and antibiotic stewardship programs. Isolates of ESBL-PE from different regions of Ethiopia were searched exhaustively. However, published data regarding the pooled estimate of ESBL-PE are not conducted in Ethiopia. For this reason, we systematically reviewed laboratory-based studies to summarize the overall pooled prevalence of the isolates recovered from various human specimens. Methods. An exhaustive literature search was carried out using the major electronic databases including PubMed, Web of Science, MEDLINE, EMBASE, CINAHL, Google Scholar, Cochrane Library, Scopus, and Wiley Online Library to identify potentially relevant studies without date restriction. Original articles which address the research question were identified, screened, and included using the PRISMA follow diagram. Data extraction form was prepared in Microsoft Excel, and data quality was assessed by using 9-point Joanna Briggs Institute critical appraisal tools. Then, data were exported to STATA 16.0 software for analyses of pooled estimation of outcome measures. Estimation of outcome measures at 95% confidence interval was performed using Der-Simonian-Laird’s random-effects model. Finally, results were presented via text, figures, and tables. Results. A comprehensive electronic database literature search has yielded a total of 86 articles. Among the total, 68 original articles were excluded after the review process. A total of 18 studies with 1191 bacterial isolates recovered from 7919 various clinical samples sizes were included for systematic review and meta-analysis. In this study, the pooled prevalence of ESBL-PE was 18% (95% CI: 9–26). Nine out of the total (50%) reviewed articles were studied using the combination disk test. Likewise, E. coli and K. pneumoniae (50% both) were the predominant isolates of ESBL-PE in addition to other isolates such as Salmonella spp. and Shigella spp. Conclusion. This meta-analysis has shown a low pooled estimate of ESBL-PE in Ethiopia.

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Detection of Extended Spectrum Beta-Lactamases Resistance Genes among Bacteria Isolated from Selected Drinking Water Distribution Channels in Southwestern Nigeria

BioMed Research International ◽

10.1155/2016/7149295 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 9

Author(s):

Ayodele T. Adesoji ◽

Adeniyi A. Ogunjobi

Keyword(s):

Resistance Genes ◽

Resistant Bacteria ◽

Beta Lactam ◽

Bacterial Strains ◽

Southwestern Nigeria ◽

Genetic Traits ◽

Beta Lactamases ◽

Esbl Gene ◽

Extended Spectrum ◽

Extended Spectrum Beta Lactamases

Extended Spectrum Beta-Lactamases (ESBL) provide high level resistance to beta-lactam antibiotics among bacteria. In this study, previously described multidrug resistant bacteria from raw, treated, and municipal taps of DWDS from selected dams in southwestern Nigeria were assessed for the presence of ESBL resistance genes which includeblaTEM,blaSHV, andblaCTXby PCR amplification. A total of 164 bacteria spread across treated (33), raw (66), and municipal taps (68), belonging toα-Proteobacteria,β-Proteobacteria,γ-Proteobacteria, Flavobacteriia, Bacilli, and Actinobacteria group, were selected for this study. Among these bacteria, the most commonly observed resistance was for ampicillin and amoxicillin/clavulanic acid (61 isolates). Sixty-one isolates carried at least one of the targeted ESBL genes withblaTEMbeing the most abundant (50/61) andblaCTXbeing detected least (3/61).Klebsiellawas the most frequently identified genus (18.03%) to harbour ESBL gene followed byProteus(14.75%). Moreover, combinations of two ESBL genes,blaSHV+blaTEMorblaCTX+blaTEM, were observed in 11 and 1 isolate, respectively. In conclusion, classicblaTEMESBL gene was present in multiple bacterial strains that were isolated from DWDS sources in Nigeria. These environments may serve as foci exchange of genetic traits in a diversity of Gram-negative bacteria.

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Detection of extended spectrum beta-lactamases and resistance in members of the Enterobacteriaceae family isolated from healthy sheep and dogs in Umuarama, Paraná, Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2016v37n2p829 ◽

2016 ◽

Vol 37 (2) ◽

pp. 829

Author(s):

Patricia Alves de Oliveira ◽

Rodrigo Assunção Moura ◽

Graziela Vendrame Rodrigues ◽

Karoline Franciani Cardoso Lopes ◽

Melissa Marchi Zaniolo ◽

...

Keyword(s):

Bacterial Resistance ◽

Brain Heart Infusion ◽

Health Concern ◽

Resistant Bacteria ◽

Human Beings ◽

Bacterial Isolates ◽

Beta Lactamases ◽

Extended Spectrum ◽

Healthy Sheep ◽

Extended Spectrum Beta Lactamases

Bacterial resistance is a primary public health concern worldwide. Within this context, pets and breeding animals act as reservoirs for multidrug-resistant bacteria (MR), such as those producing extended spectrum beta-lactamases (ESBL) and those presenting plasmid-mediated quinolone resistance (PMQR). The aim of this study was to detect the presence of ESBL and PMQR in members of the Enterobacteriaceae family, isolated from healthy sheep and dogs from non-intense farming rural properties in the Umuarama region of Paraná, Brazil. A total of 81 oral and rectal swabs from dogs and sheep from 11 small rural properties were analyzed. These swabs were inoculated in tubes containing brain heart infusion broth (BHI), and the resulting cultures were inoculated on MacConkey agar (MAC) supplemented with 10 ?g/mL cefotaxime for the selection of ESBL producers. The cells were also plated on MAC supplemented with 50 ?g/mL nalidixic acid for selecting quinolone-resistant enterobacteria. The bacterial isolates were subjected to biochemical identification tests, antibiograms, double-disk synergic tests, and polymerase chain reaction analysis for resistance-inducing genes (blaESBL, qnr, and genes encoding efflux pump and acetylases). Four (5.00%) bacterial isolates (3 Escherichia coli and 1 Morganella morganii) resistant to cephalosporins and/or quinolones were identified; of these, three (75%) isolates were from sheep and one (25%) from a dog. These findings indicate the presence of MR bacteria in the normal microbiota of the animals studied. Animals colonized with such bacteria can contribute to the dissemination of antimicrobial resistance to other animals, environment, and/or human beings and can harbor endogenous infections in unfavorable conditions, which have poor prognosis due to the limited therapeutic options.

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