Use of image analysis and rheological studies for the control of settleability of filamentous bacteria: application in SBR reactor

2001 ◽  
Vol 43 (3) ◽  
pp. 27-33 ◽  
Author(s):  
C. Dagot ◽  
M. N. Pons ◽  
M. Casellas ◽  
G. Guibaud ◽  
P. Dollet ◽  
...  
Keyword(s):  
Image Analysis ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Automated Image Analysis ◽  
Volume Index ◽  
Filamentous Bacteria ◽  
Sludge Volume Index ◽  
Floc Size ◽  
First Results

To monitor the ability of flocs to settle in Sequencing Batch Reactor sludge, two methods were tested during two operation cycles. Firstly, an automated image analysis procedure has been tested to quantify the floc size and the length and number of filaments. Secondly, rheological measurements (Bingham viscosity and shear stress) have been used to characterise the dispersion of the sludge which can reflect the cohesive strength of aggregates and so the influence of filamentous bacteria on rheological properties. These results were compared with settling parameters such as Sludge Volume Index or settling velocity. Correlations between the measured parameters with image analysis and parameters such as Sludge Volume Index have been obtained. If it is more difficult to analyze rheological results, it seems however that the thixotropy and the Bingham viscosity distinguish poor settlement owing to some filamentous bulking. The first results are promising, although they require confirmation in the long term.

Study of filamentous bacteria by image analysis and relation with settleability

2002 ◽  
Vol 46 (1-2) ◽  
pp. 363-369 ◽  
Author(s):  
M. da Motta ◽  
M.N. Pons ◽  
N. Roche
Keyword(s):  
Image Analysis ◽  
Wastewater Treatment ◽  
Settling Velocity ◽  
Treatment Plant ◽  
Volume Index ◽  
Morphological Parameters ◽  
Filamentous Bacteria ◽  
Sludge Volume Index ◽  
Floc Size ◽  
Different Temperatures

An automated procedure for the characterisation by image analysis of the morphology of activated sludge has been used to monitor the biomass in a pilot wastewater treatment plant during two runs inoculated with a different sludge and operated at two different temperatures. The bulking events were easily detected by image analysis. Correlations were found between settleability properties (Sludge Volume Index and settling velocity) and the morphological parameters (filament total length, filament number and floc size).

Cultivation of polyhydroxybutyrate-rich aerobic granular sludge in a sequencing batch reactor

2006 ◽  
Vol 6 (6) ◽  
pp. 81-87 ◽  
Author(s):  
J. Wang ◽  
H.Q. Yu
Keyword(s):  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Granular Sludge ◽  
Volume Index ◽  
Synthetic Wastewater ◽  
Aerobic Granular Sludge ◽  
Second Step ◽  
Sludge Volume Index ◽  
Flat Shape ◽  
Sludge Granulation

In this study a two-step strategy was adopted to cultivate Polyhydroxybutyrate (PHB)-rich aerobic granular sludge in a sequencing batch reactor (SBR) fed with a synthetic wastewater. In the first step both oxygen and ammonia were initially limited, in order to enhance the PHB-storage ability of sludge. In the second step granular sludge was cultivated to get a high PHB volumetric productivity. The PHB content of sludge increased to 43.1±2.0% in the first step. During the sludge granulation, the PHB content was constant at 40±4.6%. With the granulation, the settling ability of the PHB-rich sludge continuously improved, as evidenced by a decreased sludge volume index. The matured PHB-rich granular sludge presented a buff color and regular morphology with elliptical and flat shape.

On-line measurements of settling characteristics in activated sludge

1997 ◽  
Vol 36 (4) ◽  
pp. 307-312
Author(s):  
Michael R. Rasmussen ◽  
Torben Larsen
Keyword(s):  
Activated Sludge ◽  
Settling Velocity ◽  
Continuous Measurement ◽  
Batch Reactor ◽  
Volume Index ◽  
Sludge Volume Index ◽  
Dynamic Variations ◽  
On Line ◽  
Settling Column ◽  
Settling Characteristics

An on-line settling column for measuring the dynamic variations of settling velocity of activated sludge has been developed. The settling column is automatic and self-cleansing insuring continuous and reliable measurements. The settling column was tested on sludge from a batch reactor where sucrose was added as an impulse to activated sludge. The continuous measurement of settling velocity revealed a highly dynamic response after the sucrose was added. The results were verified with simultaneous measurement of the initial settling rate. A 200 hour experiment showed variations in settling velocity, which was not apparent in the DSVI (Diluted Sludge Volume Index).

Validation of a new image analysis procedure for quantifying filamentous bacteria in activated sludge

2014 ◽  
Vol 70 (6) ◽  
pp. 955-963 ◽  
Author(s):  
Ewa Liwarska-Bizukojc ◽  
Marcin Bizukojc ◽  
Olga Andrzejczak
Keyword(s):  
Image Processing ◽  
Image Analysis ◽  
Activated Sludge ◽  
Analysis Procedure ◽  
Automated Image Analysis ◽  
Filamentous Bacteria ◽  
Image Processing And Analysis ◽  
Manual Counting ◽  
Analysis Application ◽  
Activated Sludge Systems

Quantification of filamentous bacteria in activated sludge systems can be made by manual counting under a microscope or by the application of various automated image analysis procedures. The latter has been significantly developed in the last two decades. In this work a new method based upon automated image analysis techniques was elaborated and presented. It consisted of three stages: (a) Neisser staining, (b) grabbing of microscopic images, and (c) digital image processing and analysis. This automated image analysis procedure possessed the features of novelty. It simultaneously delivered data about aggregates and filaments in an individual calculation routine, which is seldom met in the procedures described in the literature so far. What is more important, the macroprogram performing image processing and calculation of morphological parameters was written in the same software which was used for grabbing of images. Previously published procedures required using two different types of software, one for image grabbing and another one for image processing and analysis. Application of this new procedure for the quantification of filamentous bacteria in the full-scale as well as laboratory activated sludge systems proved that it was simple, fast and delivered reliable results.

Long-term formation of microbial products in a sequencing batch reactor

2010 ◽  
Vol 44 (13) ◽  
pp. 3787-3796 ◽  
Author(s):  
Bing-Jie Ni ◽  
Bruce E. Rittmann ◽  
Fang Fang ◽  
Juan Xu ◽  
Han-Qing Yu
Keyword(s):  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Microbial Products

Long term operation of a granular sequencing batch reactor at pilot scale treating a low-strength wastewater

2012 ◽  
Vol 198-199 ◽  
pp. 163-170 ◽  
Author(s):  
Eduardo Isanta ◽  
María E. Suárez-Ojeda ◽  
Ángeles Val del Río ◽  
Nicolás Morales ◽  
Julio Pérez ◽  
...  
Keyword(s):  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Pilot Scale ◽  
Term Operation ◽  
Low Strength

scholarly journals Co-treatment of old landfill leachate and municipal wastewater in sequencing batch reactor (SBR): effect of landfill leachate concentration

2016 ◽  
Vol 51 (4) ◽  
pp. 377-387 ◽  
Author(s):  
Kshitij Ranjan ◽  
Shubhrasekhar Chakraborty ◽  
Mohini Verma ◽  
Jawed Iqbal ◽  
R. Naresh Kumar
Keyword(s):  
Removal Efficiency ◽  
Landfill Leachate ◽  
Suspended Solids ◽  
Sequencing Batch Reactor ◽  
Municipal Wastewater ◽  
Batch Reactor ◽  
Operating Conditions ◽  
Volume Index ◽  
Turbidity Removal ◽  
Mixed Liquor

Sequencing batch reactor (SBR) was assessed for direct co-treatment of old landfill leachate and municipal wastewater for chemical oxygen demand (COD), nutrients and turbidity removal. Nitrogen removal was achieved by sequential nitrification and denitrification under post-anoxic conditions. Initially, SBR operating conditions were optimized by varying hydraulic retention time (HRT) at 20% (v/v) landfill leachate concentration, and results showed that 6 d HRT was suitable for co-treatment. SBR performance was assessed in terms of COD, ammonia, nitrate, phosphate, and turbidity removal efficiency. pH, mixed liquor suspended solids, mixed liquor volatile suspended solids (MLVSS), and sludge volume index were monitored to evaluate stability of SBR. MLVSS indicated that biomass was able to grow even at higher concentrations of old landfill leachate. Ammonia and nitrate removal efficiency was more than 93% and 83%, respectively, whereas COD reduction was in the range of 60–70%. Phosphate and turbidity removal efficiency was 80% and 83%, respectively. Microbial growth kinetic parameters indicated that there was no inhibition of biomass growth up to 20% landfill leachate. The results highlighted that SBR can be used as an initial step for direct co-treatment of landfill leachate and municipal wastewater.

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