Comparative assessment of gene quantification using real-time PCR and water quality parameters in unsanitary landfill

2009 ◽  
Vol 59 (2) ◽  
pp. 331-338 ◽  
Author(s):  
J. S. Han ◽  
C. G. Kim

Because of increasing demanding for development of direct ecological landfill monitoring methods, there is a requirement for the condition of landfills and their influence on the environment to be characterized by the behavior of enzymes and bacteria mainly concerned with biochemical reaction in the landfills. This study was thus conducted to understand the fates of contaminants in association with groundwater quality parameters. For the study, groundwater was seasonally sampled from four closed unsanitary landfills in which microbial diversity was simultaneously obtained by 16S rDNA methods. Subsequently, a number of the specific genes of representative bacteria and encoding enzymes were quantified by real-time PCR. The relationship between water quality parameters and gene quantification were compared based on correlation factors. Correlation between DSR gene and BOD was greater than 0.8 while NSR gene and nitrate were related more than 0.9. For MTOT, it was at the highest related at 100% over BOD/COD and Dde genes were correlated over 0.8. In addition, anaerobic genes and DO were also related more than 0.8, showing anaerobic reactions generally dependent upon DO. As demonstrated in the study, molecular biological investigation and water quality parameters are highly co-linked, so that quantitative real-time PCR could be cooperatively used for assessing landfill stabilization in association with the conventional monitoring parameters.

2021 ◽  
Vol 9 (5) ◽  
pp. 474
Author(s):  
René Rodríguez-Grimón ◽  
Nestor Hernando Campos ◽  
Ítalo Braga Castro

Since 2013, there has been an increase (>23%) in naval traffic using maritime routes and ports on the coastal fringe of Santa Marta, Colombia. Of major concern, and described by several studies, is the relationship between maritime traffic and coastal contamination. This study proposed a maritime traffic indicator considering the simultaneous effects of several relevant measurements of water quality parameters to estimate the impact of naval activity. The approach involved developing a model including the number of vessels, hull length, and permanence time in berths. In addition, water quality variables, considering climatic seasons, were used to verify association with maritime traffic and touristic activities. The high concentrations of total coliforms (TC) and dissolved/dispersed petroleum hydrocarbons in chrysene equivalents (DDPH) reported by the International Marina of Santa Marta (SM) were affected by the local anthropic activities, including tourism, naval traffic, and urban wastewater discharges. Moreover, our results suggest the occurrence of multiple chemical impacts within Tayrona National Natural Park (PNNT) affecting conservation goals. The estimation of the maritime traffic indicator proposed in this study may be an easy and more complete tool for future studies evaluating the impact of naval activities on environmental quality.


Water ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1547
Author(s):  
Jian Sha ◽  
Xue Li ◽  
Man Zhang ◽  
Zhong-Liang Wang

Accurate real-time water quality prediction is of great significance for local environmental managers to deal with upcoming events and emergencies to develop best management practices. In this study, the performances in real-time water quality forecasting based on different deep learning (DL) models with different input data pre-processing methods were compared. There were three popular DL models concerned, including the convolutional neural network (CNN), long short-term memory neural network (LSTM), and hybrid CNN–LSTM. Two types of input data were applied, including the original one-dimensional time series and the two-dimensional grey image based on the complete ensemble empirical mode decomposition algorithm with adaptive noise (CEEMDAN) decomposition. Each type of input data was used in each DL model to forecast the real-time monitoring water quality parameters of dissolved oxygen (DO) and total nitrogen (TN). The results showed that (1) the performances of CNN–LSTM were superior to the standalone model CNN and LSTM; (2) the models used CEEMDAN-based input data performed much better than the models used the original input data, while the improvements for non-periodic parameter TN were much greater than that for periodic parameter DO; and (3) the model accuracies gradually decreased with the increase of prediction steps, while the original input data decayed faster than the CEEMDAN-based input data and the non-periodic parameter TN decayed faster than the periodic parameter DO. Overall, the input data preprocessed by the CEEMDAN method could effectively improve the forecasting performances of deep learning models, and this improvement was especially significant for non-periodic parameters of TN.


2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Wei Chen ◽  
Xiao Hao ◽  
JianRong Lu ◽  
Kui Yan ◽  
Jin Liu ◽  
...  

In order to solve the problems of high labor cost, long detection period, and low degree of information in current water environment monitoring, this paper proposes a lake water environment monitoring system based on LoRa and Internet of Things technology. The system realizes remote collection, data storage, dynamic monitoring, and pollution alarm for the distributed deployment of multisensor node information (water temperature, pH, turbidity, conductivity, and other water quality parameters). Moreover, the system uses STM32L151C8T6 microprocessor and multiple types of water quality sensors to collect water quality parameters in real time, and the data is packaged and sent to the LoRa gateway remotely by LoRa technology. Then, the gateway completes the bridging of LoRa link to IP link and forwards the water quality information to the Alibaba Cloud server. Finally, end users can realize the water quality control of monitored water area by monitoring management platform. The experimental results show that the system has a good performance in terms of real-time data acquisition accuracy, data transmission reliability, and pollution alarm success rate. The average relative errors of water temperature, pH, turbidity, and conductivity are 0.31%, 0.28%, 3.96%, and 0.71%, respectively. In addition, the signal reception strength of the system within 2 km is better than -81 dBm, and the average packet loss rate is only 94%. In short, the system’s high accuracy, high reliability, and long distance characteristics meet the needs of large area water quality monitoring.


2011 ◽  
Vol 383-390 ◽  
pp. 213-217 ◽  
Author(s):  
Guang Jian Chen ◽  
Jin Ling Jia

To implement the remote and real-time monitoring of surface water pollution, a design scheme of water quality monitoring system based on GPRS technology is put forward, which is composed of monitoring terminal, monitoring center and communication network. The various parameters of surface water are acquired using water quality detection sensor terminal and uploaded to the remote monitoring center via GPRS module by monitoring, and then the water quality parameters acquisition, processing and wireless transmission are realized. Water quality parameters are received through the internet network by the monitoring center, to realize its remote monitoring and management. According to the practice result, the system has materialized functions on GPRS service platform, such as real-time water quality parameters acquisition, procession, wireless transmission, remote monitoring and management, which is suitable for surface water pollution continuous monitoring and has the good application in the future.


Water ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 22
Author(s):  
Qi Cao ◽  
Gongliang Yu ◽  
Shengjie Sun ◽  
Yong Dou ◽  
Hua Li ◽  
...  

The Haihe River is a typical sluice-controlled river in the north of China. The construction and operation of sluice dams change the flow and other hydrological factors of rivers, which have adverse effects on water, making it difficult to study the characteristics of water quality change and water environment control in northern rivers. In recent years, remote sensing has been widely used in water quality monitoring. However, due to the low signal-to-noise ratio (SNR) and the limitation of instrument resolution, satellite remote sensing is still a challenge to inland water quality monitoring. Ground-based hyperspectral remote sensing has a high temporal-spatial resolution and can be simply fixed in the water edge to achieve real-time continuous detection. A combination of hyperspectral remote sensing devices and BP neural networks is used in the current research to invert water quality parameters. The measured values and remote sensing reflectance of eight water quality parameters (chlorophyll-a (Chl-a), phycocyanin (PC), total suspended sediments (TSS), total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH4-N), nitrate-nitrogen (NO3-N), and pH) were modeled and verified. The results show that the performance R2 of the training model is above 80%, and the performance R2 of the verification model is above 70%. In the training model, the highest fitting degree is TN (R2 = 1, RMSE = 0.0012 mg/L), and the lowest fitting degree is PC (R2 = 0.87, RMSE = 0.0011 mg/L). Therefore, the application of hyperspectral remote sensing technology to water quality detection in the Haihe River is a feasible method. The model built in the hyperspectral remote sensing equipment can help decision-makers to easily understand the real-time changes of water quality parameters.


Author(s):  
M. K. M. R. Guerrero ◽  
J. A. M. Vivar ◽  
R. V. Ramos ◽  
A. M. Tamondong

Abstract. The sensitivity to changes in water quality inherent to seagrass communities makes them vital for determining the overall health of the coastal ecosystem. Numerous efforts including community-based coastal resource management, conservation and rehabilitation plans are currently undertaken to protect these marine species. In this study, the relationship of water quality parameters, specifically chlorophyll-a (chl-a) and turbidity, with seagrass percent cover is assessed quantitatively. Support Vector Machine, a pixel-based image classification method, is applied to determine seagrass and non-seagrass areas from the orthomosaic which yielded a 91.0369% accuracy. In-situ measurements of chl-a and turbidity are acquired using an infinity-CLW water quality sensor. Geostatistical techniques are utilized in this study to determine accurate surfaces for chl-a and turbidity. In two hundred interpolation tests for both chl-a and turbidity, Simple Kriging (Gaussian-model type and Smooth- neighborhood type) performs best with Mean Prediction equal to −0.1371 FTU and 0.0061 μg/L, Root Mean Square Standardized error equal to −0.0688 FTU and −0.0048 μg/L, RMS error of 8.7699 FTU and 1.8006 μg/L and Average Standard Error equal to 10.8360 FTU and 1.6726 μg/L. Zones are determined using fishnet tool and Moran’s I to calculate for the seagrass percent cover. Ordinary Least Squares (OLS) is used as a regression analysis to quantify the relationship of seagrass percent cover and water quality parameters. The regression analysis result indicates that turbidity has an inverse relationship while chlorophyll-a has a direct relationship with seagrass percent cover.


Author(s):  
Wei-Jhan Syu ◽  
Tsun-Kuo Chang ◽  
Shu-Yuan Pan

In order to provide the real-time monitoring for identifying the sources of pollution and improving the irrigation water quality management, the integration of continuous automatic sampling techniques and cloud technologies is essential. In this study, we have established an automatic real-time monitoring system for improving the irrigation water quality management, especially for heavy metals such as Cd, Pb, Cu, Ni, Zn, and Cr. As a part of this work, we have first provided several examples on the basic water quality parameters (e.g., pH and electrical conductance) to demonstrate the capacity of data correction by the smart monitoring system, and then evaluated the trend and variance of water quality parameters for different types of monitoring stations. By doing so, the threshold (to initiate early warming) of different water quality parameters could be dynamically determined by the system, and the authorities could be immediately notified for follow-up actions. We have also provided and discussed the representative results from the real-time automatic monitoring system of heavy metals from different monitoring stations. Finally, we have illustrated the implications of the developed smart monitoring system for ensuring the safety of irrigation water in the near future, including integration with automatic sampling for establishing information exchange platform, estimating fluxes of heavy metals to paddy fields, and combining with green technologies for nonpoint source pollution control.


2011 ◽  
Vol 243-249 ◽  
pp. 5308-5313 ◽  
Author(s):  
Hai Yan Li ◽  
Li Tao Yue

Taking a roof in Shanghai for example, through the comparison of the relationship of rainfall and SS load in a single rainfall runoff obtained by experiment and SWMM simulation, typical water SWMM model parameters (maximum buildup possible C1, buildup rate constant C2, washoff coefficient S1 and washoff exponent S2) could be obtained. With this method, other cities’ water quality parameters for SWMM simulation could be confirmed, so as to provide basis for simulating water quality by SWMM.


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