scholarly journals Application of central composite design approach for optimization nitrate removal from aqueous solution by immobilized Pseudomonas putida

Author(s):  
Marwa E. El-Sesy ◽  
Sabah S. Ibrahim

Abstract High Nitrate concentration represented as one dangerous pollutant in the environment. Immobilization for the best denitrifying bacterial strain isolated from collected wastewater samples was suggested for bioremediation excessive nitrate concentration from aqueous solutions and explored its denitrification activity under different factors as (pH, nitrate concentration, bacterial beads, Temp and sodium alginate concentration). The active isolate was identified as Pseudomonas putida MT364822.1 by 16S rRNA analysis. Nitrate bioremediation process was optimized by apply response surface method based on central composite design approach. Nitrate uptake was significantly affected by variables of study (p-value <0.05). Maximum removal of nitrate 91.1% was obtained from pH 7, nitrate concentration 400 mg/L, immobilized bacterial beads 3.0 g/L, Temp 35 °C and sodium alginate concentration 2.5% as optimal variable values. For application, immobilized Pseudomonas putida MT364822.1 removed nitrate with 82.2% from raw fish farm effluent. Storage and reusability experiments showed the strength and stability of immobilized strain more than pure. The results suggested that, immobilized Pseudomonas putida MT364822.1 is a highly promising and suitable microorganism to be used in bio-removal of nitrate and central composite design was more effective in optimization variables to obtain the highest nitrate removal efficiency.

2016 ◽  
Vol 74 (1) ◽  
pp. 87-98 ◽  
Author(s):  
Salah T. Bouguecha ◽  
Ali Boubakri ◽  
Samir E. Aly ◽  
Mohammad H. Al-Beirutty ◽  
Mohamed M. Hamdi

Membrane distillation (MD) is considered as a relatively high-energy requirement. To overcome this drawback, it is recommended to couple the MD process with solar energy as the renewable energy source in order to provide heat energy required to optimize its performance to produce permeate flux. In the present work, an original solar energy driven direct contact membrane distillation (DCMD) pilot plant was built and tested under actual weather conditions at Jeddah, KSA, in order to model and optimize permeate flux. The dependency of permeate flux on various operating parameters such as feed temperature (46.6–63.4°C), permeate temperature (6.6–23.4°C), feed flow rate (199–451L/h) and permeate flow rate (199–451L/h) was studied by response surface methodology based on central composite design approach. The analysis of variance (ANOVA) confirmed that all independent variables had significant influence on the model (where P-value <0.05). The high coefficient of determination (R2 = 0.9644 and Radj2 = 0.9261) obtained by ANOVA demonstrated good correlation between experimental and predicted values of the response. The optimized conditions, determined using desirability function, were Tf = 63.4°C, Tp = 6.6°C, Qf = 451L/h and Qp = 451L/h. Under these conditions, the maximum permeate flux of 6.122kg/m2.h was achieved, which was close to the predicted value of 6.398kg/m2.h.


2018 ◽  
Vol 17 (2) ◽  
pp. 245-255 ◽  
Author(s):  
S. A. R. Shahamirifard ◽  
M. Ghaedi ◽  
M. Montazerozohori ◽  
A. Masoudiasl

In this work, the use of carbon dots (CDs) as a complexing agent and sensitizer in a polymeric matrix for determination of copper(ii) by UV-vis spectroscopy is reported for the first time.


2017 ◽  
Vol 109 ◽  
pp. 93-100 ◽  
Author(s):  
Chaudhry Haider Ali ◽  
Abdul Sattar Qureshi ◽  
Serge Maurice Mbadinga ◽  
Jin-Feng Liu ◽  
Shi-Zhong Yang ◽  
...  

2019 ◽  
Vol 4 (9) ◽  
pp. 149-152
Author(s):  
Andrew Ozigagun ◽  
Raphael Biu

Welding is a multi-input multi-output fabrication process, which requires a multi-response optimization technique. In this present work, the effect of heat affected zone and percentage dilution on the quality of Tungsten Inert Gas welded joints was investigated using mild steel plates. The Central Composite Design matrix was adopted to perform the welding experiment and collect the data, thereafter Response Surface Methodology (RSM) models was employed to minimize heat affected zone and percentage dilution with very significant statistical results. The result shows that the quadratic model was the most suitable for the HAZ data and the percentage dilution data with a P-value < 0.05 and R2 value of 88% and 90% for the HAZ and percentage dilution respectively.


2021 ◽  
Author(s):  
Siva Krishna Muchakayala ◽  
Kommera Pavithra ◽  
Naresh Kumar Katari ◽  
Vishnu Murthy Marisetti ◽  
THIRUPATHI DONGALA ◽  
...  

Current study portrays a specific, accurate, simple and rapid UPLC method development for the determination of impurities present in two different topical formulations (Cream and Ointment) of betamethasone dipropionate. The...


2020 ◽  
Vol 191 ◽  
pp. 164-172
Author(s):  
Adnan Ahmed ◽  
Ali Nawaz Siyal ◽  
Sumita Bhatia ◽  
Saima Qayoom Memon ◽  
Amanullah Mahar

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