Preparation and use of Ammonium Diuranate in the Ether Extraction Process

According to the national standard for the determination of tea saponin content in tea-seed pancake (GB/T 35131-2017), tea saponin was obtained by extracting oil with ethanol and removing solvent. The actual samples of tea-seed pancake did not completely remove the oil, and some of the residual oil content was as high as 9%. Due to the long period of national standard determination, people often use ethanol to extract tea saponin directly, instead of subsequent acid hydrolysis or alkali hydrolysis steps, so as to realize the rapid evaluation of tea-seed pancake quality. In this case, the residual oil has a greater impact on the results. If it was still tested according to the national standard method, the result will be much higher than the actual result. In this work, petroleum ether extraction process was selected before ethanol extraction to remove the residual oil. Experimental results showed that, the accuracy of the determination was effectively improved.

Download Full-text

Freeze-Etch Crystal-Like Structures in Membranous Glomerulosclerosis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100063883 ◽

1969 ◽

Vol 27 ◽

pp. 394-395

Author(s):

Burton B. Silver

Keyword(s):

Electron Microscopy ◽

Light Microscopy ◽

Sodium Hypochlorite ◽

Kidney Tissue ◽

Extraction Process ◽

Donor Kidney ◽

Unknown Substance ◽

Etched Surface ◽

Standard Fixation ◽

Diseased Kidney

Tissue from a non-functional kidney affected with chronic membranous glomerulosclerosis was removed at time of trnasplantation. Recipient kidney tissue and donor kidney tissue were simultaneously fixed for electron microscopy. Primary fixation was in phosphate buffered gluteraldehyde followed by infiltration in 20 and then 40% glycerol. The tissues were frozen in liquid Freon and finally in liquid nitrogen. Fracturing and replication of the etched surface was carried out in a Denton freeze-etch device. The etched surface was coated with platinum followed by carbon. These replicas were cleaned in a 50% solution of sodium hypochlorite and mounted on 400 mesh copper grids. They were examined in an Siemens Elmiskop IA. The pictures suggested that the diseased kidney had heavy deposits of an unknown substance which might account for its inoperative state at the time of surgery. Such deposits were not as apparent in light microscopy or in the standard fixation methods used for EM. This might have been due to some extraction process which removed such granular material in the dehydration steps.

Download Full-text

Biorefinery implementation for recovery debottlenecking at existing pulp mills- Part II: Techno-economic evaluation

TAPPI Journal ◽

10.32964/tj11.8.17 ◽

2012 ◽

Vol 11 (8) ◽

pp. 17-24 ◽

Cited By ~ 1

Author(s):

HAKIM GHEZZAZ ◽

LUC PELLETIER ◽

PAUL R. STUART

Keyword(s):

Cost Estimation ◽

Process Model ◽

Black Liquor ◽

Pulp Mill ◽

Value Added ◽

Economic Assessment ◽

Extraction Process ◽

Estimation Methods ◽

Precipitation Process

The evaluation and process risk assessment of (a) lignin precipitation from black liquor, and (b) the near-neutral hemicellulose pre-extraction for recovery boiler debottlenecking in an existing pulp mill is presented in Part I of this paper, which was published in the July 2012 issue of TAPPI Journal. In Part II, the economic assessment of the two biorefinery process options is presented and interpreted. A mill process model was developed using WinGEMS software and used for calculating the mass and energy balances. Investment costs, operating costs, and profitability of the two biorefinery options have been calculated using standard cost estimation methods. The results show that the two biorefinery options are profitable for the case study mill and effective at process debottlenecking. The after-tax internal rate of return (IRR) of the lignin precipitation process option was estimated to be 95%, while that of the hemicellulose pre-extraction process option was 28%. Sensitivity analysis showed that the after tax-IRR of the lignin precipitation process remains higher than that of the hemicellulose pre-extraction process option, for all changes in the selected sensitivity parameters. If we consider the after-tax IRR, as well as capital cost, as selection criteria, the results show that for the case study mill, the lignin precipitation process is more promising than the near-neutral hemicellulose pre-extraction process. However, the comparison between the two biorefinery options should include long-term evaluation criteria. The potential of high value-added products that could be produced from lignin in the case of the lignin precipitation process, or from ethanol and acetic acid in the case of the hemicellulose pre-extraction process, should also be considered in the selection of the most promising process option.

Download Full-text

Isolation of Cryptosporidium parvum oocysts from fecal samples: the combination of ether extraction and discontinuous sucrose gradients

The Korean Journal of Parasitology ◽

10.3347/kjp.1994.32.1.7 ◽

1994 ◽

Vol 32 (1) ◽

pp. 7 ◽

Cited By ~ 2

Author(s):

S H Wee ◽

C G Lee ◽

B S Kim ◽

H D Joo ◽

S W Kang

Keyword(s):

Cryptosporidium Parvum ◽

Fecal Samples ◽

Ether Extraction ◽

Cryptosporidium Parvum Oocysts

Download Full-text

Evaluation of Resistant Starch Quality from Different Types of Banana in Batter Coating Formulation to Reduce Oil Absorption in Fried Food

ICONIET PROCEEDING ◽

10.33555/iconiet.v2i2.21 ◽

2019 ◽

Vol 2 (2) ◽

pp. 115-120

Author(s):

Karissha Fritzi Della ◽

Mutiara Pratiwi ◽

Purwa Tri Cahyana ◽

Maria DPT Gunawan-Puteri

Keyword(s):

Resistant Starch ◽

Extraction Process ◽

Fat Content ◽

Water Retention Capacity ◽

Oil Absorption ◽

Retention Capacity ◽

Fried Food ◽

Musa Paradisiaca ◽

Different Types ◽

Coating Formulation

Fried food is convenient for many people due to its pleasant texture and taste. On the other hand, it comes with the risk of high oil absorption which might lead to certain health problems. Resistant starch (RS) has been known to have a functionality of reducing oil absorption. Three different types of banana: Kepok (Musa paradisiaca formatypica), Raja Bulu (Musa paradisiaca L.) and Ambon (Musa paradisiaca L. var sapientum) were evaluated on its performance when utilized as source of resistant starch especially on their application in reducing oil absorption in fried food. Tempeh was used as the food model. Banana starch (RS2) was isolated through water alkaline extraction process, continued with modification process through three repeated cycles of autoclaving-cooling process to obtain the RS3. RS3 was added into the batter coating formulation at three substitution ratios (10%, 30% and 50%) and then used to coat tempeh before frying. Evaluation of resistant starch in batter and battered productwas conducted on the following parameters: fat content, water retention capacity (WRC), coating pick up and sensory analysis. The result of this study revealed that Raja Bulu showed the most effective result on reducing oil absorption in the food tested. In the three bananas used, the ratio of 50% performed best in coating pick up (highest), WRC (highest) and fat content(lowest) parameters, but not significantly different with the 30% ratio. In terms of sensory acceptance, using Raja Bulu as the selected banana type, 30% of substitution ratio was significantly more preferable by the panelists in crispness, oiliness, and overall acceptance attributes compared to control and other substitution ratios.

Download Full-text

HAPTEN-RADIOIMMUNOASSAY OF PLASMA OESTRADIOL

Acta Endocrinologica ◽

10.1530/acta.0.0720330 ◽

1973 ◽

Vol 72 (2) ◽

pp. 330-344 ◽

Cited By ~ 10

Author(s):

Peter Doerr

Keyword(s):

Adult Male ◽

Normal Values ◽

Normal Adult ◽

Adult Males ◽

Reagent Blank ◽

Keyhole Limpet Haemocyanin ◽

Ether Extraction ◽

The Mean ◽

Assay Precision ◽

Plasma Oestradiol

ABSTRACT A hapten-radioimmunoassay for plasma oestradiol is described and information about the reliability of the method is given in detail. Oestradiol-3-hemisuccinate coupled to keyhole limpet haemocyanin is used for immunization of rabbits. The antiserum utilized for the assay is characterized by its titer, affinity and specificity. Following ether extraction and NaOH-light petroleum partition oestradiol is separated from crossreacting oestrogens by TLC. Oxidation of oestradiol on the plate is prevented by mercaptoethanol. To separate free and antibody bound ligand 250 μg dextran-coated charcoal per tube is used in the presence of bovine serum gammaglobulin (1 mg/ml). The between-assay precision based on 15 different determinations of control samples from normal adult male plasma was 9.4% (C. V.). The mean reagent blank value of 31 determinations was equivalent to 0.3 pg oestradiol and the detection limit in terms of the 99% confidence limit for a single blank value, was equivalent to 4.3 pg oestradiol. A procedure for detecting plasma blanks is described. Plasma oestradiol is separated from approximately all concomitant substances originally present in the sample by enzymatic conversion into oestrone and a second TLC. No plasma blanks could be detected with respect to normal adult male plasma. Normal values for adult males based on 51 subjects were characterized by a median of 17.2 pg/ml and the 95 percentiles of 9.5–27.6.

Download Full-text