Development and In Vitro Proof-of-Concept of Interstitially Targeted Zinc- Phthalocyanine Liposomes for Photodynamic Therapy

2013 ◽  
Vol 21 (3) ◽  
pp. 377-391 ◽  
Author(s):  
M. Broekgaarden ◽  
A. Kroon ◽  
T. Gulik ◽  
M. Heger
Keyword(s):  
Photodynamic Therapy ◽  
Zinc Phthalocyanine ◽  
Proof Of Concept

scholarly journals In vitro combined effect of Doxorubicin and sulfonated zinc Phthalocyanine–mediated photodynamic therapy on MCF-7 breast cancer cells

Tumor Biology ◽  
2017 ◽  
Vol 39 (10) ◽  
pp. 101042831772727 ◽  
Author(s):  
Eric Chekwube Aniogo ◽  
Blassan Plackal Adimuriyil George ◽  
Heidi Abrahamse
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Combined Effect ◽  
Zinc Phthalocyanine ◽  
Mcf 7

scholarly journals Anti-Psoriasis Effects and Mechanisms of Α-(8-Quinolinoxy) Zinc Phthalocyanine-Mediated Photodynamic Therapy

2017 ◽  
Vol 44 (1) ◽  
pp. 200-214 ◽  
Author(s):  
Han-Qing Liu ◽  
Ying-Ming Wang ◽  
Wan-Fang Li ◽  
Chao Li ◽  
Zhi-Huan Jiang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Mrna Expression ◽  
T Lymphocyte ◽  
B Cell Lymphoma ◽  
Zinc Phthalocyanine ◽  
Polymerase Chain Reaction Test ◽  
Therapeutic Effects ◽  
Hacat Cells

Background/Aims: The aim of this study was to determine the anti-psoriasis effects of α-(8-quinolinoxy) zinc phthalocyanine (ZnPc-F7)-mediated photodynamic therapy (PDT) and to reveal its mechanisms. Methods: HaCaT cells were used to observe the influence of ZnPc-F7-PDT on cell proliferation in vitro. The in vivo anti-psoriasis effects of ZnPc-F7-PDT were evaluated using a mouse vagina model, a propranolol-induced cavy psoriasis model and an imiquimod (IMQ)-induced nude mouse psoriasis model. Flow cytometry was carried out to determine T lymphocyte levels. Western blotting was performed to determine protein expression, and a reverse transcription-polymerase chain reaction test was performed to determine mRNA expression. Results: The results showed that ZnPc-F7-PDT significantly inhibited the proliferation of HaCaT cells in vitro; when the light doses were fixed, changing the irradiation time or output power had little influence on the inhibition rate. ZnPc-F7-PDT significantly inhibited the hyperproliferation of mouse vaginal epithelium induced by diethylstilbestrol and improved propranolol- and IMQ-induced psoriasis-like symptoms. ZnPc-F7-PDT inhibited IMQ-induced splenomegaly and T lymphocyte abnormalities. ZnPc-F7-PDT did not appear to change T lymphocytes in the mouse vagina model. ZnPc-F7-PDT down-regulated the expression of proliferating cell nuclear antigen (PCNA), B-cell lymphoma-2 (Bcl-2), interleukin (IL)-17A mRNA and IL-17F mRNA, and up-regulated the expression of Bax. Conclusion: In conclusion, ZnPc-F7-PDT exhibited therapeutic effects in psoriasis both in vitro and in vivo and is a potential approach in the treatment of psoriasis. Potential mechanisms of these effects included the inhibition of hyperproliferation; regulation of PCNA, Bcl-2, Bax, IL-17A mRNA and IL-17F mRNA expression; and immune regulation.

A monomeric photosensitizer for targeted cancer therapy

2014 ◽  
Vol 50 (95) ◽  
pp. 14983-14986 ◽  
Author(s):  
Ruizheng Liang ◽  
Lina Ma ◽  
Lele Zhang ◽  
Chunyang Li ◽  
Wendi Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Folic Acid ◽  
Cancer Therapy ◽  
Zinc Phthalocyanine ◽  
Targeted Cancer Therapy

A targeted photosensitizer used in photodynamic therapy (PDT) was fabricated by incorporation of zinc phthalocyanine (ZnPc) and folic acid (FA) into polyvinylpyrrolidone (PVP) micelles, which exhibits excellent anticancer performance revealed by both in vitro studies and in vivo tests.

Monomer zinc phthalocyanine/upconversion nanoparticle coated with hyaluronic acid crosslinked gel as NIR light-activated drug for in vitro photodynamic therapy

2016 ◽  
Vol 45 (38) ◽  
pp. 15170-15179 ◽  
Author(s):  
Lin Zhou ◽  
Enyi Chen ◽  
Weiwei Jin ◽  
Yue Wang ◽  
Jiahong Zhou ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Hyaluronic Acid ◽  
Anticancer Activity ◽  
Zinc Phthalocyanine ◽  
Nir Light

A monomeric phthalocyanine based NIR-triggered cancer target nanosystem was prepared and showed satisfied in vitro anticancer activity.

Amphiphilic zinc phthalocyanine photosensitizers: synthesis, photophysicochemical properties and in vitro studies for photodynamic therapy

2015 ◽  
Vol 44 (20) ◽  
pp. 9646-9658 ◽  
Author(s):  
Dilek Çakır ◽  
Meltem Göksel ◽  
Volkan Çakır ◽  
Mahmut Durmuş ◽  
Zekeriya Biyiklioglu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vitro Studies ◽  
Zinc Phthalocyanine

scholarly journals EFFECT OF THE ZINC PHTHALOCYANINE MEDIATED PHOTODYNAMIC THERAPY ON CYTOSKELETAL APPARATUS OF HELA CELLS

2019 ◽  
Vol 49 (2) ◽  
pp. 41-45
Author(s):  
Barbora Hošíková ◽  
Svatopluk Binder ◽  
René Lenobel ◽  
Jakub Malohlava ◽  
Jakub Hošík ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescent Microscopy ◽  
Tumor Cell Line ◽  
Zinc Phthalocyanine ◽  
Atomic Force ◽  
Cell Line Hela ◽  
Therapy Effect ◽  
Cervical Cancer Cells ◽  
Microscopy Techniques

<p class="Abstract">This study deals with the utilization of photosensitizer (λmax ~ 660 nm) from the group of the phthalocyanines, in photodynamic therapy. Effect of the zinc phthalocyanine photosensitizer mediated photodynamic therapy was evaluated in vitro on the tumor cell line – HeLa (cervical cancer cells) using mass spectrometry and atomic force and fluorescent microscopy techniques.</p>

Water-soluble non-aggregating zinc phthalocyanine and in vitro studies for photodynamic therapy

2013 ◽  
Vol 49 (95) ◽  
pp. 11149 ◽  
Author(s):  
Saad Makhseed ◽  
Miloslav Machacek ◽  
Waleed Alfadly ◽  
Ahmad Tuhl ◽  
Mickey Vinodh ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Water Soluble ◽  
In Vitro Studies ◽  
Zinc Phthalocyanine

scholarly journals Multiorganelle Localization of Metallated Phthalocyanine Photosensitizer in Colorectal Cancer Cells (DLD-1 and CaCo-2) Enhances Efficacy of Photodynamic Therapy

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Palesa Rose Sekhejane ◽  
Nicolette Nadene Houreld ◽  
Heidi Abrahamse
Keyword(s):  
Colorectal Cancer ◽  
Cell Death ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Apoptotic Cell ◽  
Morphological Changes ◽  
Annexin V ◽  
Zinc Phthalocyanine ◽  
Cell Death Pathway

Colorectal cancer is the third most commonly diagnosed cancer. Amongst treatments that have been explored, photodynamic therapy (PDT) is a treatment that is of interest as it poses ideal advantages such as affinity for cancer cells. This study aimed to determine the correlation between the localization site of a sulfonated zinc phthalocyanine (ZnPcSmix) photosensitizer (PS) and its associated cell death pathwayin vitroin colorectal cancer cell lines (DLD-1 and CaCo-2). Visible morphological changes were observed in PDT treated cells after 24 h. Reactive oxygen species (ROS) were detected and visualized 1 h after PDT.ZnPcSmixwas predominantly localized in lysosomes and partially in the mitochondria. FITC Annexin V staining showed a significant decrease in the percentage of viable DLD-1 and CaCo-2 cells 24 h after PDT, with an increase in apoptotic cell population. Moreover, there was a significant increase in both cathepsin D and cytochrome C at 1 and 24 h. In conclusion,ZnPcSmixshowed the ability of inducing apoptotic cell death features in PDT treated cells.

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