scholarly journals EFFECT OF THE ZINC PHTHALOCYANINE MEDIATED PHOTODYNAMIC THERAPY ON CYTOSKELETAL APPARATUS OF HELA CELLS

2019 ◽  
Vol 49 (2) ◽  
pp. 41-45
Author(s):  
Barbora Hošíková ◽  
Svatopluk Binder ◽  
René Lenobel ◽  
Jakub Malohlava ◽  
Jakub Hošík ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescent Microscopy ◽  
Tumor Cell Line ◽  
Zinc Phthalocyanine ◽  
Atomic Force ◽  
Cell Line Hela ◽  
Therapy Effect ◽  
Cervical Cancer Cells ◽  
Microscopy Techniques

<p class="Abstract">This study deals with the utilization of photosensitizer (λmax ~ 660 nm) from the group of the phthalocyanines, in photodynamic therapy. Effect of the zinc phthalocyanine photosensitizer mediated photodynamic therapy was evaluated in vitro on the tumor cell line – HeLa (cervical cancer cells) using mass spectrometry and atomic force and fluorescent microscopy techniques.</p>

The investigation of in vitro dark cytotoxicity and photodynamic therapy effect of a 2,6-dibromo-3,5-distyryl BODIPY dye encapsulated in Pluronic® F-127 micelles

2018 ◽  
Vol 71 (21) ◽  
pp. 3444-3457 ◽  
Author(s):  
Nthabeleng Molupe ◽  
Balaji Babu ◽  
David O. Oluwole ◽  
Earl Prinsloo ◽  
John Mack ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Therapy Effect

scholarly journals Biological Atomic Force Microscopy for Imaging Gold-Labeled Liposomes on Human Coronary Artery Endothelial Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Ana-María Zaske ◽  
Delia Danila ◽  
Michael C. Queen ◽  
Eva Golunski ◽  
Jodie L. Conyers
Keyword(s):  
Atomic Force Microscopy ◽  
Endothelial Cells ◽  
Coronary Artery ◽  
Cell Membrane ◽  
Fluorescent Microscopy ◽  
Human Coronary Artery ◽  
Force Microscopy ◽  
Cell Internalization ◽  
Atomic Force

Although atomic force microscopy (AFM) has been used extensively to characterize cell membrane structure and cellular processes such as endocytosis and exocytosis, the corrugated surface of the cell membrane hinders the visualization of extracellular entities, such as liposomes, that may interact with the cell. To overcome this barrier, we used 90 nm nanogold particles to label FITC liposomes and monitor their endocytosis on human coronary artery endothelial cells (HCAECs) in vitro. We were able to study the internalization process of gold-coupled liposomes on endothelial cells, by using AFM. We found that the gold-liposomes attached to the HCAEC cell membrane during the first 15–30 min of incubation, liposome cell internalization occurred from 30 to 60 min, and most of the gold-labeled liposomes had invaginated after 2 hr of incubation. Liposomal uptake took place most commonly at the periphery of the nuclear zone. Dynasore monohydrate, an inhibitor of endocytosis, obstructed the internalization of the gold-liposomes. This study showed the versatility of the AFM technique, combined with fluorescent microscopy, for investigating liposome uptake by endothelial cells. The 90 nm colloidal gold nanoparticles proved to be a noninvasive contrast agent that efficiently improves AFM imaging during the investigation of biological nanoprocesses.

scholarly journals In vitro combined effect of Doxorubicin and sulfonated zinc Phthalocyanine–mediated photodynamic therapy on MCF-7 breast cancer cells

Tumor Biology ◽  
2017 ◽  
Vol 39 (10) ◽  
pp. 101042831772727 ◽  
Author(s):  
Eric Chekwube Aniogo ◽  
Blassan Plackal Adimuriyil George ◽  
Heidi Abrahamse
Keyword(s):  
Breast Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Combined Effect ◽  
Zinc Phthalocyanine ◽  
Mcf 7

Autocrine IL6/STAT3 Signaling Enhances Survival of Chronic Lymphocytic Leukaemia Cells

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 3598-3598
Author(s):  
Fengting Liu ◽  
Li Jia ◽  
Timothy Farren ◽  
John G. Gribben ◽  
Samir Agrawal
Keyword(s):  
Gene Transfection ◽  
Fluorescent Microscopy ◽  
Ros Generation ◽  
Apoptosis Resistance ◽  
Cytochrome C Release ◽  
Cancer Cell Survival ◽  
High Level ◽  
Stat3 Signalling ◽  
Microscopy Techniques

Abstract Abstract 3598 Signal transducer and activator of transcription 3 (STAT3) proteins have been found to play an important role in cancer cell survival and proliferation. The activation of STAT3 signalling pathways require interleukin-6 (IL6) and tyrosine kinase (JAK2) phosphorylation. Previous studies have shown that in patients with CLL elevated levels of serum IL6 correlate with adverse disease features and shorter survival. Methods: We investigated the relationship of CLL cell autocrine IL6 production, STAT3 activation and apoptosis resistance. Thirty-six CLL patients with high lymphocyte counts were investigated. Westen blot, flow cytometry, gene transfection and fluorescent microscopy techniques were used. Results: Autocrine IL6 production in CLL cells cultured for 24 hours was higher in Binet stage B/C patients (61pg/ml, range 1.4–297pg/ml) as compared with stage A patients (6.1pg/ml, range 0–23pg/ml) (p=0.02). Patients with high autocrine IL6 production had a higher ratio of phosphorylated STAT3/ total STAT3, indicating a high level of STAT3 activation and apoptosis resistance. Activation of the IL6/JAK2/STAT3 pathway by exogenous IL6 led to increased expression of anti-apoptotic proteins Mcl-1 and Bcl-xl. STAT3 activation by exogenous IL6 led to increased resistance of CLL cells to spontaneous in vitro apoptosis (mean increase in viable cells over control: 15%, range 2–36%; p=0.00008). This was associated with preservation of mitochondrial function: decreased cytochrome C release (p=0.004), mitochondrial membrane potential collapse (p=0.0004) and ROS generation (p=0.0007). This study demonstrates that higher autocrine IL6 production by CLL correlates with STAT3 activation and apoptosis resistance in CLL. The IL6/JAK2/STAT3 signal pathway may reveal new therapeutic targets. Disclosures: Gribben: Roche: Consultancy; Celgene: Consultancy; GSK: Honoraria; Napp: Honoraria.

scholarly journals Redox-Sensitive and Folate-Receptor-Mediated Targeting of Cervical Cancer Cells for Photodynamic Therapy Using Nanophotosensitizers Composed of Chlorin e6-Conjugated β-Cyclodextrin via Diselenide Linkage

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2190
Author(s):  
Howard Kim ◽  
Mi Woon Kim ◽  
Young-IL Jeong ◽  
Hoe Saeng Yang
Keyword(s):  
Cervical Cancer ◽  
Photodynamic Therapy ◽  
Hela Cells ◽  
Folate Receptor ◽  
Chlorin E6 ◽  
Ros Generation ◽  
Cervical Cancer Cells ◽  
Poly Ethylene

The aim of this study was to fabricate a reactive oxygen species (ROS)-sensitive and folate-receptor-targeted nanophotosensitizer for the efficient photodynamic therapy (PDT) of cervical carcinoma cells. Chlorin e6 (Ce6) as a model photosensitizer was conjugated with succinyl β-cyclodextrin via selenocystamine linkages. Folic acid (FA)-poly(ethylene glycol) (PEG) (FA-PEG) conjugates were attached to these conjugates and then FA-PEG-succinyl β-cyclodextrin-selenocystamine-Ce6 (FAPEGbCDseseCe6) conjugates were synthesized. Nanophotosensitizers of FaPEGbCDseseCe6 conjugates were fabricated using dialysis membrane. Nanophotosensitizers showed spherical shapes with small particle sizes. They were disintegrated in the presence of hydrogen peroxide (H2O2) and particle size distribution changed from monomodal distribution pattern to multimodal pattern. The fluorescence intensity and Ce6 release rate also increased due to the increase in H2O2 concentration, indicating that the nanophotosensitizers displayed ROS sensitivity. The Ce6 uptake ratio, ROS generation and cell cytotoxicity of the nanophotosensitizers were significantly higher than those of the Ce6 itself against HeLa cells in vitro. Furthermore, the nanophotosensitizers showed folate-receptor-specific delivery capacity and phototoxicity. The intracellular delivery of nanophotosensitizers was inhibited by folate receptor blocking, indicating that they have folate-receptor specificity in vitro and in vivo. Nanophotosensitizers showed higher efficiency in inhibition of tumor growth of HeLa cells in vivo compared to Ce6 alone. These results show that nanophotosensitizers of FaPEGbCDseseCe6 conjugates are promising candidates as PDT of cervical cancer.

scholarly journals Anti-Psoriasis Effects and Mechanisms of Α-(8-Quinolinoxy) Zinc Phthalocyanine-Mediated Photodynamic Therapy

2017 ◽  
Vol 44 (1) ◽  
pp. 200-214 ◽  
Author(s):  
Han-Qing Liu ◽  
Ying-Ming Wang ◽  
Wan-Fang Li ◽  
Chao Li ◽  
Zhi-Huan Jiang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Mrna Expression ◽  
T Lymphocyte ◽  
B Cell Lymphoma ◽  
Zinc Phthalocyanine ◽  
Polymerase Chain Reaction Test ◽  
Therapeutic Effects ◽  
Hacat Cells

Background/Aims: The aim of this study was to determine the anti-psoriasis effects of α-(8-quinolinoxy) zinc phthalocyanine (ZnPc-F7)-mediated photodynamic therapy (PDT) and to reveal its mechanisms. Methods: HaCaT cells were used to observe the influence of ZnPc-F7-PDT on cell proliferation in vitro. The in vivo anti-psoriasis effects of ZnPc-F7-PDT were evaluated using a mouse vagina model, a propranolol-induced cavy psoriasis model and an imiquimod (IMQ)-induced nude mouse psoriasis model. Flow cytometry was carried out to determine T lymphocyte levels. Western blotting was performed to determine protein expression, and a reverse transcription-polymerase chain reaction test was performed to determine mRNA expression. Results: The results showed that ZnPc-F7-PDT significantly inhibited the proliferation of HaCaT cells in vitro; when the light doses were fixed, changing the irradiation time or output power had little influence on the inhibition rate. ZnPc-F7-PDT significantly inhibited the hyperproliferation of mouse vaginal epithelium induced by diethylstilbestrol and improved propranolol- and IMQ-induced psoriasis-like symptoms. ZnPc-F7-PDT inhibited IMQ-induced splenomegaly and T lymphocyte abnormalities. ZnPc-F7-PDT did not appear to change T lymphocytes in the mouse vagina model. ZnPc-F7-PDT down-regulated the expression of proliferating cell nuclear antigen (PCNA), B-cell lymphoma-2 (Bcl-2), interleukin (IL)-17A mRNA and IL-17F mRNA, and up-regulated the expression of Bax. Conclusion: In conclusion, ZnPc-F7-PDT exhibited therapeutic effects in psoriasis both in vitro and in vivo and is a potential approach in the treatment of psoriasis. Potential mechanisms of these effects included the inhibition of hyperproliferation; regulation of PCNA, Bcl-2, Bax, IL-17A mRNA and IL-17F mRNA expression; and immune regulation.

An in vitro study of biological safety of condoms and their additives

2003 ◽  
Vol 22 (12) ◽  
pp. 659-664 ◽  
Author(s):  
N A Motsoane ◽  
M J Bester ◽  
E Pretorius ◽  
P J Becker
Keyword(s):  
Hela Cell ◽  
Cell Viability ◽  
Cell Line ◽  
Tumor Cell Line ◽  
Toxic Effects ◽  
Specific Cell ◽  
Epithelial Tumor ◽  
Hela Cell Lines ◽  
Cell Line Hela

The use of condoms to prevent sexually transmitted diseases, especially HIV, is widely encouraged. Condoms contain latex, nonspermicidal lubricants (such as dimethylsiliconium) and other nonspecified compounds, such as colorants and flavorings. Latex causes allergy reaction in susceptible individuals but little is known regarding the cytotoxic effects of other additives. The objective of this study was to develop a sensitive in vitrosystem to determine the toxic effects of condom material. The modified L929 FDA method and a more specific cell type, such as the cervical epithelial tumor cell line HeLa, was used. Lubricated (LC), lubricated and flavored (LFC), and lubricated, flavored and colored condoms (LFCC) were evaluated. Washings containing condom surface material were prepared by washing condom fragments in medium for different time intervals. Changes in cell number, viability and lysosome integrity in the L929 and HeLa cell lines was determined using the Crystal Violet, MTT and Neutral Red assays, respectively. The condom type affected cell viability and lysosome integrity, with LC inducing an increase in cell viability and LFC a decrease in lysosome integrity. The HeLa cell line in combination with the MTT and NR assay was the most sensitive in vitro system to determine the toxic effects of condom material.

5-Aminolevulinic acid-based photodynamic therapy of chordoma: In vitro experiments on a human tumor cell line

2017 ◽  
Vol 20 ◽  
pp. 111-115 ◽  
Author(s):  
Jan F. Cornelius ◽  
Lennert Eismann ◽  
Lara Ebbert ◽  
Brigitte Senger ◽  
Athanasios K. Petridis ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Tumor Cell ◽  
Cell Line ◽  
Aminolevulinic Acid ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Human Tumor Cell Line ◽  
In Vitro Experiments ◽  
Human Tumor Cell
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