Synthesis and Characterization of Folic Acid Conjugated Gemcitabine Tethered Silver Nanoparticles (FA-GEM-AgNPs) for Targeted Delivery

2020 ◽  
Vol 26 (26) ◽  
pp. 3141-3146 ◽  
Author(s):  
Arjunan Karuppaiah ◽  
Ravikumar Rajan ◽  
Sivaram Hariharan ◽  
Dinesh K. Balasubramaniam ◽  
Marslin Gregory ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Folic Acid ◽  
Cancer Cells ◽  
Targeted Delivery ◽  
Cytotoxic Effect ◽  
Apoptotic Cell ◽  
Folate Receptor ◽  
Cancer Cell Line ◽  
One Pot

Background: Silver nanoparticles (AgNPs) have attracted considerable interest in the medical industry due to their physicochemical properties, small size, and surface plasmon behavior. Their smaller particle size and instability in blood circulation leads to toxicity due to its aggregation as Ag+ ions and accumulation at the deepseated organ. In the present study, we aimed at reducing the toxicity of AgNPs by conjugation with an anticancer drug GEM and to improve their internalization through folate receptors-mediated endocytosis by capping the nanoparticles with folic acid (FA). Methods: One-pot facile synthesis of FA capped silver nanoparticles (FA-AgNPs) has been achieved by using FA as a reducing agent. FA-AgNPs were mixed with Gemcitabine (GEM) to obtain tethered FA-GEM-AgNPs. Nanoparticles were characterized by Dynamic Light Scattering (DLS), UV-Visible spectroscopy, Transmission Electron Microscopy (TEM), Energy Dispersive X-ray Analysis (EDAX), Selected Area Electron Diffraction (SAED), and Atomic Absorption Spectroscopy (AAS). The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was carried out to determine the cytotoxic effect of the prepared nanoformulations. The apoptotic cell death induced by FA-GEM-AgNPs in breast cancer cells were monitored with Acridine orange (AO)/Ethidium Bromide (EtBr) staining. Conclusion: Compared to GEM and AgNPs, FA-GEM-AgNPs showed enhanced cytotoxic effect and internalization in MDA-MB-453 breast cancer cell line. FA-GEM-AgNPs could be an ideal candidate for targeting cancer cells via folate receptor-mediated endocytosis.

scholarly journals Folate receptor targeted delivery of paclitaxel to breast cancer cells via folic acid conjugated graphene oxide grafted methyl acrylate nanocarrier

2019 ◽  
Vol 110 ◽  
pp. 906-917 ◽  
Author(s):  
Kandasamy Vinothini ◽  
Naresh Kumar Rajendran ◽  
Andy Ramu ◽  
Nandhakumar Elumalai ◽  
Mariappan Rajan
Keyword(s):  
Breast Cancer ◽  
Graphene Oxide ◽  
Folic Acid ◽  
Cancer Cells ◽  
Methyl Acrylate ◽  
Targeted Delivery ◽  
Breast Cancer Cells ◽  
Folate Receptor

Design, In Silico Modelling, and Functionality Theory of Novel Folate Receptor Targeted Rutin Encapsulated Folic Acid Conjugated Keratin Nanoparticles for Effective Cancer Treatment

2020 ◽  
Vol 19 (16) ◽  
pp. 1966-1982 ◽  
Author(s):  
Selvaraj Kunjiappan ◽  
Theivendren Panneerselvam ◽  
Saravanan Govindaraj ◽  
Pavadai Parasuraman ◽  
Suraj Baskararaj ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Cell Death ◽  
Folic Acid ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Folate Receptor ◽  
Release Kinetics ◽  
Covalent Binding ◽  
Mcf 7

Objective: Site-specific and toxic-free drug delivery, is an interesting area of research. Nanoengineered drug delivery systems possess a remarkable potential for effective treatment of various types of cancers. Methods: In this study, novel Folic Acid (FA) conjugated keratin nanoparticles (NPs) were assembled with encapsulation and delivery of Rutin (Rt) into breast cancer cells through the overexpressed folate receptor. The biocompatible, Rt encapsulated FA conjugated keratin NPs (FA@Ker NPs) were successfully formulated by a modified precipitation technique. Their morphological shape and size, size distribution, stability, and physical nature were characterized and confirmed. The drug (Rt) encapsulation efficiency, loading capacity and release kinetics were also studied. Results: The observed results of molecular docking and density functionality theory of active drug (Rt) showed a strong interaction and non-covalent binding of the folate receptor and facilitation of endocytosis in breast cancer cells. Further, in vitro cytotoxic effect of FA@Ker NPs was screened against MCF-7 cancer cells, at 55.2 µg/mL of NPs and found to display 50% of cell death at 24h. Moreover, the NPs enhanced the uptake of Rt in MCF-7 cells, and the apoptotic effect of condensed nuclei and distorted membrane bodies was observed. Also, NPs entered into the mitochondria of MCF-7 cells and significantly increased the level of ROS which led to cell death. Conclusion: The developed FA@Ker NPs might be a promising way to enhance anti-cancer activity without disturbing normal healthy cells.

RGD peptide-mediated liposomal curcumin targeted delivery to breast cancer cells

2020 ◽  
pp. 088532822094936
Author(s):  
Reza Mahmoudi ◽  
Seyedeh Ashraf Mirahmadi-Babaheidri ◽  
Hamdollah Delaviz ◽  
Mohamad Hassan Fouani ◽  
Mohsen Alipour ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Targeted Delivery ◽  
Cytotoxic Effect ◽  
Cancer Cell Line ◽  
Control Group ◽  
Mcf 7

In this study, turmeric's active ingredient (Curcumin) was encapsulated into RGD modified Liposomes (RGD-Lip-Cur) its cytotoxic effect on the breast cancer cell line (MCF-7) was evaluated by MTT, flow cytometry and Caspase assay. Liposomes were characterized using transmission electron microscopy (TEM). Results demonstrated that the liposomes were spherical in shape, ranging from 70 to 100 nm. MTT assay revealed that RGD-Lip-Cur had a significant cytotoxic effect on MCF-7 cells at concentrations of 32, 16 and 4 μg/ml compared to Lip-Cur (P < 0.05) and curcumin (P < 0.01). The apoptosis assay demonstrated that RGD-Lip-Cur induces the apoptosis in MCF-7 cells (39.6% vs 40.2% for initial and secondary apoptosis) significantly more than Lip-Cur (67.7% vs 9.16% for initial and secondary apoptosis) and free curcumin (7.84% vs 38.8% for initial and secondary apoptosis). Moreover, caspase assay showed that RGD-Lip-Cur activates caspase 3/7 compared to Lip-Cur (P < 0.05) and free curcumin (P < 0.01). The RGD-Lip-Cur was similar to the control group and had no significant cytotoxicity effect. It is concluded that RGD-Lip-Cur as a novel carrier have high cytotoxicity effect on breast cancer cell line (MCF-7).

scholarly journals Folic Acid-Functionalized, Condensed Magnetic Nanoparticles for Targeted Delivery of Doxorubicin to Tumor Cancer Cells Overexpressing the Folate Receptor

ACS Omega ◽  
2019 ◽  
Vol 4 (26) ◽  
pp. 22214-22227 ◽  
Author(s):  
Athina Angelopoulou ◽  
Argiris Kolokithas-Ntoukas ◽  
Christos Fytas ◽  
Konstantinos Avgoustakis
Keyword(s):  
Folic Acid ◽  
Magnetic Nanoparticles ◽  
Cancer Cells ◽  
Targeted Delivery ◽  
Folate Receptor

Cytotoxic effect of green synthesized silver nanoparticles in MCF7 and MDA-MB-231 human breast cancer cells in vitro

The Nucleus ◽  
2019 ◽  
Vol 63 (2) ◽  
pp. 191-202 ◽  
Author(s):  
Arindam Bandyopadhyay ◽  
Bishnupada Roy ◽  
Pallab Shaw ◽  
Paritosh Mondal ◽  
Maloy Kr. Mondal ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Cytotoxic Effect ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Green Synthesized Silver Nanoparticles

scholarly journals CYTOTOXIC EFFECT OF PEPTIDE FUNCTIONALIZED SILVER NANOPARTICLES SYNTHESIZED FROM ALOIN ON BREAST CANCER CELL LINE

2019 ◽  
pp. 170-175
Author(s):  
LEEMA ROSE A ◽  
VIDHYA S ◽  
JANEETA PRIYA F ◽  
SABEENA ASHIFA J
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Ag Nanoparticles ◽  
Cytotoxic Effect ◽  
Cancer Cell Line ◽  
Uv Visible

Objective: The aim of the present study is to synthesize the silver (Ag) nanoparticle using Aloin with a focus on its antibacterial, antioxidant, and anticancer activity. Methods: The silver nanoparticles were synthesized using Aloin and were determined by UV-Visible spectrum. It was further characterized by scanning electron microscope (SEM), zeta potential, and dynamic light scattering (DLS). The Fourier transform infrared analysis was also carried out for the Aloin. Results: The UV-Visible absorption spectrum of the synthesized silver nanoparticles has shown the absorption peak at 439nm which proves the formation of silver nanoparticles in the solution. The SEM analysis revealed that the Ag nanoparticles were spherical in shape. The IR spectra showed that there are 6 functional groups are present in Aloin extract. The synthesized nanoparticles are found to be highly stable with an average particle size of 130.7nm which was confirmed by zeta potential and DLS analysis. The synthesized nanoparticles had a good antibacterial and antioxidant activity. It shows a very good cytotoxic effect against breast cancer cell line. Conclusion: The present study suggests that the synthesis route is free from the requirements such as high energy, extended preparation time, and special equipments and thus can be used for large-scale synthesis in food industries for food preservation and these Ag nanoparticles can be used for its therapeutic purposes for developing a new drug against cancer.

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