Antibacterial Effect of Gallic Acid against Aeromonas hydrophila and Aeromonas sobria Through Damaging Membrane Integrity

2016 ◽  
Vol 17 (13) ◽  
pp. 1153-1158 ◽  
Author(s):  
Jing Lu ◽  
Zhenning Wang ◽  
Mengrou Ren ◽  
Guoren Huang ◽  
Baochen Fang ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Aeromonas Hydrophila ◽  
Antibacterial Effect ◽  
Membrane Integrity ◽  
Aeromonas Sobria

Aeromonas hydrophila, Aeromonas sobria, MDR, Mugil fish and Nile tilapia

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
asmaa arafat ◽  
Omnia ELkilany ◽  
Haidy Abdel-Rahman
Keyword(s):  
Aeromonas Hydrophila ◽  
Nile Tilapia ◽  
Aeromonas Sobria

scholarly journals Isolation and identification of motile Aeromonas spp.from different sources

2011 ◽  
Vol 5 (3) ◽  
pp. 56-65
Author(s):  
Samira Y. Yousif ◽  
Rasha Abid Ali Al-Khalidi
Keyword(s):  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Morphological Characterization ◽  
Isolation And Identification ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae ◽  
Susceptibility Tests ◽  
Different Sources

Total of 507 samples (clinical, environmental, food) were collected from different hospitals in Baghdad, water, soil, and different food stuffs. Biochemical and morphological characterization tests showed that seventeen isolates were identified as Aeromonas spp.These were farther characterized as Aeromonas hydrophila 10 isolates, Aeromonas sobria 2 isolates, Aeromonas eucrenophila 3 isolates, one isolate belongs to Aeromonas caviae and another one belongs to Aeromonas schubertii. Antibiotic susceptibility tests of all the isolates towards fifteen antibiotics agents were carried out and results showed that all isolates 100% were resistant to penicillin, ampicillin, ampiclox, 99% were resistant to lincomycin, 76.7% to cephalothin, 52.9% to cefotaxime. All isolates except one isolate of Aeromonas eucrenophila were sensitive to meropenem.

Influence of Carvacrol and 1,8-Cineole on Cell Viability, Membrane Integrity, and Morphology of Aeromonas hydrophila Cultivated in a Vegetable-Based Broth

2015 ◽  
Vol 78 (2) ◽  
pp. 424-429 ◽  
Author(s):  
JOSSANA PEREIRA de SOUSA ◽  
KATARYNE ÁRABE RIMÁ de OLIVEIRA ◽  
REGINA CELIA BRESSAN QUEIROZ de FIGUEIREDO ◽  
EVANDRO LEITE de SOUZA
Keyword(s):  
Cell Viability ◽  
Membrane Permeability ◽  
Aeromonas Hydrophila ◽  
Membrane Integrity ◽  
Cell Membrane Permeability ◽  
Confocal Laser ◽  
Wall Structure ◽  
Bacterial Cells ◽  
Transmission Electron ◽  
Cytoplasmic Content

This study investigated the effects of carvacrol (CAR) and 1,8-cineole (CIN) alone (at the MIC) or in combination at subinhibitory amounts (both at 1/8 MIC) on the cell viability, membrane permeability, and morphology of Aeromonas hydrophila INCQS 7966 (A. hydrophila) cultivated in a vegetable-based broth. CAR and CIN alone or in combination severely affected the viability of the bacteria and caused dramatic changes in the cell membrane permeability, leading to cell death, as observed by confocal laser microscopy. Scanning and transmission electron microscopy images of bacterial cells exposed to CAR or CIN or the mixture of both compounds revealed severe changes in cell wall structure, rupture of the plasma membrane, shrinking of cells, condensation of cytoplasmic content, leakage of intracellular material, and cell collapse. These findings suggest that CAR and CIN alone or in combination at subinhibitory amounts could be applied to inhibit the growth of A. hydrophila in foods, particularly as sanitizing agents in vegetables.

Low Incidence of Aeromonas sp. in Livestock Feces

1987 ◽  
Vol 50 (1) ◽  
pp. 66-69 ◽  
Author(s):  
NORMAN J. STERN ◽  
E. S. DRAZEK ◽  
S. W. JOSEPH
Keyword(s):  
Aeromonas Hydrophila ◽  
Recovery Rate ◽  
Agricultural Research ◽  
Agar Medium ◽  
Brain Heart Infusion ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae ◽  
Retail Meat ◽  
Agar Media ◽  
Low Incidence

Pig, beef, sheep and turkey fecal specimens were assayed for recovery of inoculated Aeromonas sp. by directly plating the samples on five different agar media. Of these, starch-ampicillin was optimal with respect to selectivity and ability to differentiate from other resident microflora. Generally, the numbers of inoculated Aeromonas sp. recovered on starch-ampicillin agar were similar to those recovered on brain heart infusion and blood ampicillin agar media, and were 101 to 103 greater than the recovery rate on either MacConkey-ampicillin or cefsulodinirgasan-novobiocin agars. The sensitivity for the direct recovery of Aeromonas sp. from inoculated beef feces with naturally contaminating microflora, using streaked starch-ampicillin agar medium, was between 102 and 103 cells per gram. Using starch-ampicillin agar, the incidence of Aeromonas detected from feces of beef, pig, sheep and turkey held at the Beltsville Agricultural Research Center was one of 32, none of 22, none of 24 and three of 21, respectively. Based upon current taxonomic criteria, the isolate from the beef feces had characteristics consistent with both Aeromonas sobria and Aeromonas caviae, whereas three isolates from turkey feces were identified as A. caviae or Aeromonas hydrophila. The organism was isolated from five of five packages of ground beef from retail sources. The discrepancy in the consistent presence of the organism in retail meat suggests that many of the food isolates are probably not of fecal origin.

Survival of Aeromonas hydrophila, Aeromonas caviae and Aeromonas sobria in soil

1996 ◽  
Vol 81 (4) ◽  
pp. 439-444 ◽  
Author(s):  
G. Brandi ◽  
M. Sisti ◽  
G. F. Schiavano ◽  
L. Salvaggio ◽  
A. Albano
Keyword(s):  
Aeromonas Hydrophila ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae

scholarly journals Enterotoxic activity of Aeromonas hydrophila and Aeromonas sobria

1983 ◽  
Vol 57 (10) ◽  
pp. 853-861
Author(s):  
Kazuhiro KOBAYASHI ◽  
Kazuhiro HARADA ◽  
Toshio SHIMADA ◽  
Riichi SAKAZAKI
Keyword(s):  
Aeromonas Hydrophila ◽  
Aeromonas Sobria

scholarly journals Antibacterial Activity of Cuttlefish Sepia sp. (Cephalopoda,) Ink Extract Against Aeromonas hydrophila

2019 ◽  
Vol 24 (3) ◽  
pp. 184
Author(s):  
R Adharyan Islamy
Keyword(s):  
Antibacterial Activity ◽  
Aeromonas Hydrophila ◽  
Antibacterial Effect ◽  
Methanolic Extract ◽  
Positive Control ◽  
Average Yield ◽  
Antibacterial Test ◽  
Treatment Dose ◽  
Dried Extract ◽  
Antiretroviral Activity

Cephalopods ink has shown potential antiretroviral activity. The ink extracts of cuttlefish showed an antibacterial effect. This study aims to investigate the antibacterial activity of the methanolic extract of the ink of cuttlefish Sepia sp. against Aeromonas hydrophila. A. hydrophila are opportunists and associated with aquatic fish and shrimp disease. The shade-dried ink sample from approximately 30g ink sacs obtained from 15 animals was immersed separately in methanol (1:3 w/v) solvents for overnight. The experiment in this study used the dried extract of cuttlefish ink. The isolate of A. hydrophila was originated from Jepara Brackishwater Aquaculture Center. The average yield percentage of cuttlefish ink extract obtained was 4.86%. The results of the MIC test in Table V show that the highest average absorbance value obtained was at a concentration of 50 ppm, which was equal to 1.716 nm, and the lowest absorbance was obtained at a treatment dose of 300 ppm at 0.841 nm, while the Mc Farland tube was 0.933 nm. The results of antibacterial test on Table II showed antibacterial activity of cuttlefish ink extract at negative concentration control showed diameter zone of 5 ± 1.2 mm, at positive control showed diameter zone of 31 ± 1.2 mm, at 250 ppm result 19 ± 0.9 mm, at 300 ppm result 22 ± 1.4 mm, at 350 ppm result 31 ± 1.2 mm.

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