Searching for Potential Biomarkers of Penthorum chinense Pursh by Extraction Method and HPLC-MS Analysis Integrated with Evaluation of Hepatoprotective Effect

2018 ◽  
Vol 14 (4) ◽  
pp. 389-396 ◽  
Author(s):  
Jin-Yi Wan ◽  
Zong-Liang Sun ◽  
Yu-Zhen Zhang ◽  
Cai-Bing Wei ◽  
Chong-Zhi Wang ◽  
...  
Molecules ◽  
2015 ◽  
Vol 20 (4) ◽  
pp. 6443-6453 ◽  
Author(s):  
Yangyang Hu ◽  
Shengpeng Wang ◽  
Anqi Wang ◽  
Ligen Lin ◽  
Meiwan Chen ◽  
...  

2015 ◽  
Vol 6 (5) ◽  
pp. 1510-1517 ◽  
Author(s):  
Yi-Wei Cao ◽  
Yun Jiang ◽  
Da-Yong Zhang ◽  
Xiao-Jing Zhang ◽  
Yuan-Jia Hu ◽  
...  

Aqueous extracts ofPenthorum chinensePursh, a health food and folk medicine, protects against acute alcohol-induced liver injury.


2020 ◽  
Vol 11 (9) ◽  
pp. 7925-7934
Author(s):  
Yi-Chao Du ◽  
Li Lai ◽  
Hao Zhang ◽  
Fu-Rui Zhong ◽  
Huan-Li Cheng ◽  
...  

In this study, kaempferol (KA) ameliorates APAP-induced hepatotoxicity by activating HO-1/NQO1 and inhibiting HMGB1/TLR4/NF-κB signaling and NLRP3 inflammasome activation.


2021 ◽  
Vol 19 (1) ◽  
pp. 653-664
Author(s):  
Grażyna Kowalska ◽  
Radosław Kowalski

Abstract The objective of this study was the estimation of the content of 13 mycotoxins (diacetoxyscirpenol, T-2 toxin, HT-2 toxin, nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, fusarenone X, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, and zearalenone) in various products from the eastern part of Poland. The content of mycotoxins in the analysed samples was assayed using the extraction method combined with HPLC-MS/MS analysis. We found mycotoxins in 25 of the 92 samples tested (27%). Contamination with mycotoxins was noted most frequently in samples of cereals – 56% – and also in samples of flour and cocoa, in which a content of mycotoxins was noted in 24 and 16% of the samples, respectively. The most frequently identified were the following – deoxynivalenol detected in 18 samples (72%), zearalenone detected in eight samples (32%), toxin HT-2 detected in four samples (16%), ochratoxin A identified in three samples (12%), and toxin T-2 detected in one sample (4%). In one analysed sample of mixed flour and in one analysed sample of wheat and rye flour, the maximum allowable concentration was exceeded in the case of two identified mycotoxins – deoxynivalenol (2,250 μg/kg) and ochratoxin A (15.6 and 17.1 μg/kg).


2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Li-mei Lin ◽  
Ling-jia Zhao ◽  
Jing Deng ◽  
Su-hui Xiong ◽  
Jie Tang ◽  
...  

Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.


2010 ◽  
Vol 1 (1) ◽  
Author(s):  
Scott Counts ◽  
Elliott Mufson

AbstractThe identification of individuals at risk for Alzheimer’s disease (AD) is essential for the timely administration of treatment approaches aimed at slowing the onset or progression of the disease. As amnestic forms of mild cognitive impairment (aMCI) may represent preclinical AD, the search for specific diagnostic biomarkers that characterize those with aMCI is a key research objective. Using surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDITOF-MS), we screened the cerebrospinal fluid (CSF) of Religious Orders Study participants with a clinical diagnosis of no cognitive impairment (NCI), aMCI, or mild/moderate AD for potential biomarkers. CSF was fractionated on immobilized metal affinity chromatography (IMAC) protein arrays preloaded with either gallium (IMAC-Ga), which binds phosphoproteins, or copper (IMAC-Cu) to isolate copper-binding proteins. SELDI TOF-MS analysis of the IMAC-Ga arrays revealed a phosphopeptide of 2490 Da that was selectively increased ∼2-fold in aMCI and AD CSF compared to NCI. SELDI TOF-MS analysis of the IMAC-Cu arrays identified 2 proteins of 11.7 and 13.3 kDa that were both selectively increased ∼1.5–1.6-fold in aMCI and AD CSF. Increasing levels of each protein were associated with poorer performance on the Mini Mental State Exam and higher Braak stage. Hence, increased CSF levels of these proteins may be potential biomarkers for preclinical AD and aid in the development of a CSF biomarker panel with high predictive value for identifying people who would most benefit from early therapeutic interventions to modify disease progression.


2015 ◽  
Vol 11 ◽  
pp. 163-167 ◽  
Author(s):  
Doudou Huang ◽  
Yun Jiang ◽  
Wansheng Chen ◽  
Fengyan Yao ◽  
Dan Xue ◽  
...  

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