Application of graphene oxide-based hydrogels in bone tissue engineering

: This paper provides a review on the applications of some graphene oxide-based hydrogels in bone tissue engineering. Hydrogels used in bone tissue engineering usually require suitable biocompatibility, porosity, and mechanical strength. By adding GO, under the original hydrogel condition, the various indexes of the composite hydrogel can reach more suitable conditions for inducing bone defect healing. This article also introduces some high-performance graphene oxide-based hydrogels, which are likely to play an essential role in bone tissue engineering in the future.

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A review of biomaterials in bone defect healing, remaining shortcomings and future opportunities for bone tissue engineering

Bone and Joint Research ◽

10.1302/2046-3758.73.bjr-2017-0270.r1 ◽

2018 ◽

Vol 7 (3) ◽

pp. 232-243 ◽

Cited By ~ 132

Author(s):

T. Winkler ◽

F. A. Sass ◽

G. N. Duda ◽

K. Schmidt-Bleek

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Regeneration ◽

Bone Tissue ◽

Bone Defect ◽

Bone Healing ◽

Lessons Learned ◽

Form And Function ◽

Defect Healing ◽

Bone Defect Healing

Despite its intrinsic ability to regenerate form and function after injury, bone tissue can be challenged by a multitude of pathological conditions. While innovative approaches have helped to unravel the cascades of bone healing, this knowledge has so far not improved the clinical outcomes of bone defect treatment. Recent findings have allowed us to gain in-depth knowledge about the physiological conditions and biological principles of bone regeneration. Now it is time to transfer the lessons learned from bone healing to the challenging scenarios in defects and employ innovative technologies to enable biomaterial-based strategies for bone defect healing. This review aims to provide an overview on endogenous cascades of bone material formation and how these are transferred to new perspectives in biomaterial-driven approaches in bone regeneration. Cite this article: T. Winkler, F. A. Sass, G. N. Duda, K. Schmidt-Bleek. A review of biomaterials in bone defect healing, remaining shortcomings and future opportunities for bone tissue engineering: The unsolved challenge. Bone Joint Res 2018;7:232–243. DOI: 10.1302/2046-3758.73.BJR-2017-0270.R1.

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Three-dimensional porous scaffold by self-assembly of reduced graphene oxide and nano-hydroxyapatite composites for bone tissue engineering

Carbon ◽

10.1016/j.carbon.2017.02.013 ◽

2017 ◽

Vol 116 ◽

pp. 325-337 ◽

Cited By ~ 107

Author(s):

Wei Nie ◽

Cheng Peng ◽

Xiaojun Zhou ◽

Liang Chen ◽

Weizhong Wang ◽

...

Keyword(s):

Tissue Engineering ◽

Graphene Oxide ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Reduced Graphene Oxide ◽

Self Assembly ◽

Porous Scaffold ◽

Three Dimensional ◽

Reduced Graphene

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Silicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineering

Journal of Biomaterials Applications ◽

10.1177/0885328218763665 ◽

2018 ◽

Vol 32 (10) ◽

pp. 1392-1405 ◽

Cited By ~ 15

Author(s):

Ali Deniz Dalgic ◽

Ammar Z. Alshemary ◽

Ayşen Tezcaner ◽

Dilek Keskin ◽

Zafer Evis

Keyword(s):

Tissue Engineering ◽

Graphene Oxide ◽

Bone Tissue Engineering ◽

Protein Adsorption ◽

Bone Tissue ◽

Three Dimensional ◽

Osteosarcoma Cell ◽

In Vitro Biocompatibility ◽

Microscopy Techniques

In this study, novel graphene oxide–incorporated silicate-doped nano-hydroxyapatite composites were prepared and their potential use for bone tissue engineering was investigated by developing an electrospun poly(ε-caprolactone) scaffold. Nanocomposite groups were synthesized to have two different ratios of graphene oxide (2 and 4 wt%) to evaluate the effect of graphene oxide incorporation and groups with different silicate-doped nano-hydroxyapatite content was prepared to investigate optimum concentrations of both silicate-doped nano-hydroxyapatite and graphene oxide. Three-dimensional poly(ε-caprolactone) scaffolds were prepared by wet electrospinning and reinforced with silicate-doped nano-hydroxyapatite/graphene oxide nanocomposite groups to improve bone regeneration potency. Microstructural and chemical characteristics of the scaffolds were investigated by X-ray diffraction, Fourier transform infrared spectroscope and scanning electron microscopy techniques. Protein adsorption and desorption on material surfaces were studied using fetal bovine serum. Presence of graphene oxide in the scaffold, dramatically increased the protein adsorption with decreased desorption. In vitro biocompatibility studies were conducted using human osteosarcoma cell line (Saos-2). Electrospun scaffold group that was prepared with effective concentrations of silicate-doped nano-hydroxyapatite and graphene oxide particles (poly(ε-caprolactone) – 10% silicate-doped nano-hydroxyapatite – 4% graphene oxide) showed improved adhesion, spreading, proliferation and alkaline phosphatase activity compared to other scaffold groups.

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Enhanced Cell Proliferation and Osteogenesis Differentiation through a Combined Treatment of Poly-L-Lysine-Coated PLGA/Graphene Oxide Hybrid Fiber Matrices and Electrical Stimulation

Journal of Nanomaterials ◽

10.1155/2020/5892506 ◽

2020 ◽

Vol 2020 ◽

pp. 1-15

Author(s):

Jiaqi Zhu ◽

Zhiping Qi ◽

Changjun Zheng ◽

Pan Xue ◽

Chuan Fu ◽

...

Keyword(s):

Tissue Engineering ◽

Cell Proliferation ◽

Graphene Oxide ◽

Electrical Stimulation ◽

Bone Tissue Engineering ◽

Bone Regeneration ◽

Bone Tissue ◽

Cellular Response ◽

Hybrid Fiber ◽

Fiber Matrices

Bone tissue engineering scaffold provides an effective treatment for bone defect repair. Biodegradable bone scaffold made of various synthetic and natural materials can be used as bone substitutes and grafts for defect site, which has great potential to support bone regeneration. Regulation of cell-scaffold material interactions is an important factor for modulating the cellular activity in bone tissue engineering scaffold applications. Thus, the hydrophilic, mechanical, and chemical properties of scaffold materials directly affect the results of bone regeneration and functional recovery. In this study, a poly-L-lysine (PLL) surface-modified poly(lactic-co-glycolic acid) (PLGA)/graphene oxide (GO) (PLL-PLGA/GO) hybrid fiber matrix was fabricated for bone tissue regeneration. Characterization of the resultant hybrid fiber matrices was done using scanning electron microscopy (SEM), contact angle, and a material testing machine. According to the results obtained from the test above, the PLL-PLGA/GO hybrid fiber matrices exhibited high wettability and mechanical strength. The special surface characteristics of PLL-PLGA/GO hybrid fiber matrices were more beneficial for protein adsorption and inhibit the proliferation of pathogens. Moreover, the enhanced regulation of MC3T3-E1 cell proliferation and differentiation was observed, when the resultant hybrid fiber matrices were combined with electrical stimulation (ES). The cellular response of MC3T3-E1 cells including cell adhesion, proliferation, alkaline phosphatase (ALP) activity, calcium deposition, and osteogenesis-related gene expression was significantly enhanced with the synergistic effect of resultant hybrid fiber matrices and ES. These data indicate that the PLL-PLGA/GO hybrid fiber matrices supported the cellular response in terms of cell proliferation and osteogenesis differentiation in the presence of electrical stimulation, which could be a potential treatment for bone defect.

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Preparation and biological properties of silk fibroin/nano-hydroxyapatite/graphene oxide scaffolds with an oriented channel-like structure

RSC Advances ◽

10.1039/c9ra09710d ◽

2020 ◽

Vol 10 (17) ◽

pp. 10118-10128 ◽

Cited By ~ 4

Author(s):

Lu Wang ◽

Min Fang ◽

Yijing Xia ◽

Jiaxin Hou ◽

Xiaoru Nan ◽

...

Keyword(s):

Tissue Engineering ◽

Graphene Oxide ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Silk Fibroin ◽

Biological Properties ◽

Hybrid Scaffold

A novel SF/nHAp/GO hybrid scaffold with oriented channel-like structure in bone tissue engineering.

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Development of a nanocomposite scaffold of gelatin–alginate–graphene oxide for bone tissue engineering

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2019.04.113 ◽

2019 ◽

Vol 133 ◽

pp. 592-602 ◽

Cited By ~ 31

Author(s):

Shiv Dutt Purohit ◽

Rakesh Bhaskar ◽

Hemant Singh ◽

Indu Yadav ◽

Mukesh Kumar Gupta ◽

...

Keyword(s):

Tissue Engineering ◽

Graphene Oxide ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Nanocomposite Scaffold

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Development of new graphene oxide incorporated tricomponent scaffolds with polysaccharides and hydroxyapatite and study of their osteoconductivity on MG-63 cell line for bone tissue engineering

RSC Advances ◽

10.1039/c5ra07015e ◽

2015 ◽

Vol 5 (51) ◽

pp. 41135-41143 ◽

Cited By ~ 27

Author(s):

R. Rajesh ◽

Y. Dominic Ravichandran

Keyword(s):

Tissue Engineering ◽

Graphene Oxide ◽

Bone Tissue Engineering ◽

Cell Line ◽

Bone Tissue ◽

Gellan Gum ◽

First Time

GO–alginate–HAP, GO–amylopectin–HAP and GO–gellan gum–HAP were prepared and characterized and their osteoconductivity were checked for the first time.

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