ROS-scavenging and Anti-tyrosinase Properties of Crocetin on B16F10 Murine Melanoma Cells

2018 ◽  
Vol 18 (7) ◽  
pp. 1064-1069 ◽  
Author(s):  
Seyed H. Hashemi-Shahri ◽  
Alireza Golshan ◽  
Seyed A. Mohajeri ◽  
Javad Baharara ◽  
Elaheh Amini ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Melanoma Cells ◽  
Biological Properties ◽  
B16 Melanoma ◽  
Tyrosinase Activity ◽  
Crocus Sativus ◽  
Mushroom Tyrosinase ◽  
Ros Scavenging ◽  
Protein Levels ◽  
B16 Melanoma Cells

Background: Crocus sativus (Iridaceae) has been traditionally used in the Iranian folk medicine and as a culinary additive. Major components of the plant that are responsible for biological properties are saffranal, crocin, picrocrocin and crocetin. Although the level of crocetin is not high, some of the important activities of saffron such as antioxidant activity have been attributed to crocetin. Objective: In the present study, we investigated the effects of crocetin on melanogenesis in B16 melanoma cells. Methods: The effect of crocetin on intracellular and mushroom tyrosinase activity and the content of melanin was evaluated spectrophotometrically. Tyrosinase and Microphthalmia-Associated Transcription Factor (MITF) protein levels were compared between Crocetin-treated and control cells after western blot analysis. The antioxidative activity of crocetin was also investigated. Results: Crocetin could inhibit mushroom tyrosinase activity and lower the amount of melanin in B16 melanoma cells. Protein levels of tyrosinase and MITF were also decreased by crocetin. Crocetin also showed antioxidant activity and depleted cellular Reactive Oxygen Species (ROS) content but had no cytotoxicity in alamarBlue® assay. Conclusion: Taken together, decreased tyrosinase activity, melanin content, tyrosinase and MITF proteins levels, and ROS production showed the inhibition of melanogenesis in B16F10 cells by crocetin. Hence, crocetin could be suggested as a potential dermatological whitening agent in skin care products.

scholarly journals Osmanthus fragrans Flower Aqueous Extract and its Enriched Acteoside inhibit Melanogenesis and Ultraviolet-induced Pigmentation

2018 ◽  
Vol 13 (5) ◽  
pp. 1934578X1801300
Author(s):  
Shuo Liu ◽  
Zhen Zhao ◽  
Zhijun Huo ◽  
Zhiru Xu ◽  
Yan Zhong ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Ex Vivo ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Tyrosinase Activity ◽  
In Vivo Model ◽  
Mrna Levels ◽  
Osmanthus Fragrans ◽  
B16 Melanoma Cells

The Osmanthus fragrans flower (OFF) is commonly used as an additive for tea in China and as a traditional medicine to treat dysentery, asthma and hepatitis. In the current study, we have acquired the aqueous extract of the dried OFF (OFFE) and determined its enriched acteoside contents. However, whether OFFE and acteoside can modulate melanogenesis and pigmentation has yet to be determined. We here provide novel data revealing that OFFE and acteoside inhibit melanogenesis induced by α-MSH in B16 melanoma cells via the MITF-tyrosinase signaling pathway. Treatment with α-MSH (1μM) enhanced melanin levels and tyrosinase activity, up-regulated the mRNA levels of MITF and tyrosinase and increased the dendritic number in B16 melanoma cells, effects all being intervened by OFFE and acteoside. Of interest, OFFE and acteoside showed no direct inhibition of tyrosinase activity as revealed by our ex vivo tyrosinase activity assay. In addition, OFFE produced a depigmenting action on UVB-induced hyperpigmentation in guinea pigs, as shown by the improved skin brightness and the decreased melanin staining. Our data have demonstrated that OFFE can alter melanogenesis via modulating the MITF-tyrosinase signaling thereby leading to its depigmenting action in the in vivo model. OFFE could be a substitute for acteoside as a promising skin-whitening agent.

scholarly journals The Effects of Salacia reticulata on Anti-Cellular Oxidants and Melanogenesis Inhibition in α-MSH-stimulated and UV Irradiated B16 Melanoma Cells

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900
Author(s):  
Prasit Sirwannalert ◽  
Ryusho Kariya ◽  
Ikuko Suzu ◽  
Seiji Okada
Keyword(s):  
Melanoma Cells ◽  
Pharmaceutical Products ◽  
B16 Melanoma ◽  
Tyrosinase Activity ◽  
Root Extract ◽  
Dependent Manner ◽  
Inhibitory Effects ◽  
B16 Melanoma Cells ◽  
Dose Dependent ◽  
Salacia Reticulata

The purposes of this study were to investigate the inhibitory effects of Salacia reticulata Tul. root extract on cellular oxidants and melanogenesis in B16 melanoma cells. Cells treated with non-toxic doses of S. reticulata root extract were investigated for their effects on melanogenesis, cellular tyrosinase activity and cellular oxidant scavenging activity. The results indicated that S. reticulata extract inhibited melanin synthesis and tyrosinase activity in α-MSH-induced or UV-irradiated B16 melanoma cells in a dose dependent manner. Additionally, the extract also exhibited anti-cellular oxidants in UV-induced radical melanoma cells. Altogether, these results suggested that S. reticulata root extract has roles in suppression of melanogenesis and oxidant inhibition. S. reticulata root extract may be a potential source for the development of pharmaceutical products for treatment of skin hyperpigmentation disorders.

Effects of Salicylic Acid on Mushroom Tyrosinase and B16 Melanoma Cells

2007 ◽  
Vol 62 (3-4) ◽  
pp. 227-233 ◽  
Author(s):  
Sadaaki Tamura ◽  
Teruhiko Nitoda ◽  
Isao Kubo
Keyword(s):  
Salicylic Acid ◽  
Reaction Time ◽  
Initial Rate ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Enzymatic Oxidation ◽  
Hydroxybenzoic Acid ◽  
Mushroom Tyrosinase ◽  
B16 Melanoma Cells

Abstract Salicylic acid slightly inhibited the oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) catalyzed by mushroom tyrosinase noncompetitively without being oxidized. In contrast, 4-hydroxybenzoic acid did not inhibit this enzymatic oxidation if a longer reaction time was observed, although it suppressed the initial rate of the oxidation to a certain extent. Neither acid showed noticeable effects on cultured murine B16-F10 melanoma cells except weak cytotoxicity.

Heat Shock Protein Levels Are Not Elevated in Heat-Resistant B16 Melanoma Cells

1986 ◽  
Vol 105 (2) ◽  
pp. 240 ◽  
Author(s):  
Robin L. Anderson ◽  
Tien Wen Tao ◽  
David A. Betten ◽  
George M. Hahn
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Protein Levels ◽  
B16 Melanoma Cells ◽  
Heat Resistant

Suppression of the melanogenesis and cellular antioxidant activity in B16 melanoma cells

2010 ◽  
Vol 32 (6) ◽  
pp. 471-471 ◽  
Author(s):  
A. Tsuda ◽  
S. Horigome ◽  
I. Yoshida ◽  
A. Yamaguchi ◽  
N. Kibune ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Cellular Antioxidant Activity ◽  
B16 Melanoma Cells

scholarly journals Tyrosinase activity and melanogenic effects of Lespedeza bicolor extract in vitro and in vivo

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 6244-6261
Author(s):  
Si Young Ha ◽  
Ji Young Jung ◽  
Hee Young Kang ◽  
Tae-Heung Kim ◽  
Jae-Kyung Yang
Keyword(s):  
Ethanol Extract ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Tyrosinase Activity ◽  
Melanin Content ◽  
B16 Melanoma Cells ◽  
Notable Increase ◽  
Lespedeza Bicolor

Lespedeza bicolor (L. bicolor) is used for medicinal purposes because of its various biological and pharmacological activities. In this study, the effects of L. bicolor ethanol extract on the treatment of vitiligo were investigated. The determination of melanin content in melanocytes was measured using B16 melanoma cells and C57BL/6J Ler-vit/vit mice. Finally, the quercetin content in L. bicolor were qualitatively analyzed using HPLC. The results obviously indicated that the L. bicolor extract enhanced melanogenesis and increased tyrosinase activity in cultured melanoma cells and C57BL/6J Ler-vit/vit mice. Treatment with L. bicolor extract led to a higher content of melanin and eumelanin in C57BL/6J Ler-vit/vit mice hair than in control (untreated) mice, which demonstrated the therapeutic effect of hair-graying associated with vitiligo. There was a notable increase in melanocytes in the skin of C57BL/6J Ler-vit/vit mice treated with L. bicolor extract compared with the control. L. bicolor extract was a potent tyrosinase and melanin synthesis activator in B16 melanoma cells. C57BL/6J Ler-vit/vit mice treated with L. bicolor extract had significantly higher melanin content in hair than the untreated control. The results suggest that L. bicolor extract is a potential alternative treatment for improvement of vitiligo.

The effect of chlorophorin and its derivative on melanin biosynthesis

Holzforschung ◽  
2005 ◽  
Vol 59 (5) ◽  
pp. 514-518 ◽  
Author(s):  
Enos Tangke Arung ◽  
Keisuke Yoshikawa ◽  
Kuniyoshi Shimizu ◽  
Ryuichiro Kondo
Keyword(s):  
Inhibitory Activity ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Mushroom Tyrosinase ◽  
Tyrosinase Inhibitor ◽  
Melanin Biosynthesis ◽  
B16 Melanoma Cells ◽  
Bioassay Guided Fractionation ◽  
Unstable Compound ◽  
Potent Inhibitory Activity

Abstract By means of bioassay-guided fractionation using mushroom tyrosinase, a geranylated stilbene, chlorophorin, was characterized as the principal tyrosinase inhibitor in the heartwood of Chlorophora excelsa (Moraceae). It inhibited the oxidation of L-tyrosine and DL-3,4-dihydroxyphenylalanine (DL-DOPA) due to mushroom tyrosinase and melanin biosynthesis on B16 melanoma cells. Chlorophorin, which is a slight yellowish compound, has previously been reported as an unstable compound in light. On the basis of this finding, a chlorophorin derivative [4-(3″,7″-dimethyloctyl)-2′,3,4′,5-tetrahydroxydihydrostilbene; hexahydrochlorophorin] which is colorless, obtained by the hydrogenation of chlorophorin with Pd/C, was also tested to develop a superior material for practical use. Hexahydrochlorophorin showed more potent inhibitory activity on tyrosinase and melanin biosynthesis, and lower cytotoxicity towards B16 melanoma cells than chlorophorin.

Inhibition of melanogenesis by BMY-28565, a novel compound depressing tyrosinase activity in B16 melanoma cells

1992 ◽  
Vol 43 (2) ◽  
pp. 183-189 ◽  
Author(s):  
Terao Mineko ◽  
Tomita Koji ◽  
Oki Toshikazu ◽  
Linda Tabe ◽  
Maurizio Gianni ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
B16 Melanoma ◽  
Tyrosinase Activity ◽  
B16 Melanoma Cells

Effects of volatile oil from ginger on the murine B16 melanoma cells and its mechanism

2018 ◽  
Vol 9 (2) ◽  
pp. 1058-1069 ◽  
Author(s):  
Li-Xia Wang ◽  
Jia Qian ◽  
Li-Nan Zhao ◽  
Shao-Hua Zhao
Keyword(s):  
Antioxidant Properties ◽  
Volatile Oil ◽  
Melanoma Cells ◽  
B16 Melanoma ◽  
Tyrosinase Activity ◽  
Inhibitory Effects ◽  
B16 Melanoma Cells ◽  
Related Proteins

Volatile oil from ginger suppressed melanogenesis through its antioxidant properties and the inhibitory effects on tyrosinase activity and melanogensis-related proteins.

Sign in / Sign up

Export Citation Format

Share Document