scholarly journals Evaluation in Mouse Model of Combined Virus-bacterial Vaccine Based on Attenuated Influenza A(H7N3) Virus and the Group B Streptococcus Recombinant Polypeptides

2016 ◽  
Vol 10 (1) ◽  
pp. 168-175 ◽  
Author(s):  
Yulia A. Desheva ◽  
Galina F. Leontieva ◽  
Tatiana A. Kramskaya ◽  
Tatiana A. Smolonogina ◽  
Kornelia B. Grabovskaya ◽  
...  
Keyword(s):  
Mouse Model ◽  
Influenza Vaccine ◽  
Influenza A ◽  
Group B Streptococcus ◽  
Surface Proteins ◽  
Wild Type Virus ◽  
Common Cause ◽  
Intranasal Immunization ◽  
Group B ◽  
Recombinant Polypeptides

Background:Secondary bacterial influenza complications are a common cause of excesses morbidity and mortality, which determines the need to develop means for specific prophylaxis. Group B streptococcal infection is especially common cause of pneumonia among children and the elderly with underlying conditions. Here we investigate in a mouse model the effects of combined intranasal immunization using live attenuated influenza vaccine and recombinant polypeptides based on group BStreptococcussurface proteins.Methods:Groups of outbred mice received two doses of the following preparations: 1) the reassortant A/17/Mallard/Netherlands/00/95 (H7N3) influenza virus; 2) a mixture of P6, ScaAB, ScpB1 and Stv recombinant GBS proteins (20 µg total); 3) the A(H7N3) influenza vaccine pooled with the four bacterial peptide preparation; 4) control animals were treated with PBS.Results:Intranasal vaccination using LAIV in combination with GBS polypeptides provided advantageous protection against infections with homologous A/Mallard/Netherlands/12/00 (H7N3) wild type virus or heterologous A/Puerto Rico/8/34 (H1N1) followed by serotype II GBS infection. Also, combined vaccination improved bacterial clearance from the lungs of mice.Conclusion:Intranasal immunization with LAIV+GBSV was safe and enabled to induce the antibody response to each of vaccine components. Thus, the combined vaccine increased the protective effect against influenza and its bacterial complications in mice compared to LAIV-only.

scholarly journals Prevention of Influenza A(H7N9) and Bacterial Infections in Mice Using Intranasal Immunization With Live Influenza Vaccine and the Group B Streptococcus Recombinant Polypeptides

2017 ◽  
Vol 8 ◽  
pp. 1178122X1771094 ◽  
Author(s):  
Yulia A Desheva ◽  
Galina F Leontieva ◽  
Tatiana A Kramskaya ◽  
Tatiana A Smolonogina ◽  
Kornelia B Grabovskaya ◽  
...  
Keyword(s):  
Influenza Vaccine ◽  
Messenger Rna ◽  
Influenza A ◽  
Bacterial Infections ◽  
Influenza Infection ◽  
Group B Streptococcus ◽  
Infectious Dose ◽  
Intranasal Immunization ◽  
Group B ◽  
Recombinant Polypeptides

We investigate the protective effect of combined vaccination based on live attenuated influenza vaccine (LAIV) and group B streptococcus (GBS) recombinant polypeptides against potential pandemic H7N9 influenza infection followed by GBS burden. Mice were intranasally immunized using 107 50% egg infectious dose (EID50) of H7N3 LAIV, the mix of the 4 GBS peptides (group B streptococcus vaccine [GBSV]), or combined LAIV + GBSV vaccine. The LAIV raised serum hemagglutination-inhibition antibodies against H7N9 in higher titers than against H7N3. Combined vaccination provided advantageous protection against infections with A/Shanghai/2/2013(H7N9)CDC-RG influenza and serotype II GBS. Combined vaccine significantly improved bacterial clearance from the lungs after infection compared with other vaccine groups. The smallest lung lesions due to combined LAIV + GBSV vaccination were associated with a prevalence of lung interferon-γ messenger RNA expression. Thus, combined viral and bacterial intranasal immunization using H7N3 LAIV and recombinant bacterial polypeptides induced balanced adaptive immune response, providing protection against potential pandemic influenza H7N9 and bacterial complications.

scholarly journals Association between antibodies against group B Streptococcus surface proteins and recto-vaginal colonisation during pregnancy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Sonwabile Dzanibe ◽  
Gaurav Kwatra ◽  
Peter V. Adrian ◽  
Sheila Z. Kimaro-Mlacha ◽  
Clare L. Cutland ◽  
...  
Keyword(s):  
Group B Streptococcus ◽  
Surface Proteins ◽  
Group B

scholarly journals Intranasal Immunization of Mice with Group B Streptococcal Protein Rib and Cholera Toxin B Subunit Confers Protection against Lethal Infection

2004 ◽  
Vol 72 (2) ◽  
pp. 1184-1187 ◽  
Author(s):  
Charlotte Larsson ◽  
Jan Holmgren ◽  
Gunnar Lindahl ◽  
Charlotta Bergquist
Keyword(s):  
Cholera Toxin ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Intranasal Immunization ◽  
Cholera Toxin B ◽  
B Subunit ◽  
A Cell ◽  
Group B

ABSTRACT Intranasal immunization of mice with Rib, a cell surface protein of group B streptococcus (GBS), conjugated to or simply coadministered with the recombinant cholera toxin B subunit, induces systemic immunoglobulin G (IgG) and local IgA antibody responses and confers protection against lethal GBS infection. These findings have implications for the development of a human GBS vaccine.

scholarly journals Identification of Major Outer Surface Proteins of Streptococcus agalactiae

2002 ◽  
Vol 70 (3) ◽  
pp. 1254-1259 ◽  
Author(s):  
Martin J. G. Hughes ◽  
Joanne C. Moore ◽  
Jonathan D. Lane ◽  
Rebecca Wilson ◽  
Philippa K. Pribul ◽  
...  
Keyword(s):  
Outer Surface ◽  
Streptococcus Agalactiae ◽  
Expression System ◽  
Group B Streptococcus ◽  
Phosphoglycerate Kinase ◽  
Peptide Sequencing ◽  
Surface Proteins ◽  
Outer Surface Proteins ◽  
Group B

ABSTRACT To identify the major outer surface proteins of Streptococcus agalactiae (group B streptococcus), a proteomic analysis was undertaken. An extract of the outer surface proteins was separated by two-dimensional electrophoresis. The visualized spots were identified through a combination of peptide sequencing and reverse genetic methodologies. Of the 30 major spots identified as S. agalactiae specific, 27 have been identified. Six of these proteins, previously unidentified in S. agalactiae, were sequenced and cloned. These were ornithine carbamoyltransferase, phosphoglycerate kinase, nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, purine nucleoside phosphorylase, enolase, and glucose-6-phosphate isomerase. Using a gram-positive expression system, we have overexpressed two of these proteins in an in vitro system. These recombinant, purified proteins were used to raise antisera. The identification of these proteins as residing on the outer surface was confirmed by the ability of the antisera to react against whole, live bacteria. Further, in a neonatal-animal model system, we demonstrate that some of these sera are protective against lethal doses of bacteria. These studies demonstrate the successful application of proteomics as a technique for identifying vaccine candidates.

Serotype and surface protein gene distribution of colonizing group B streptococcus in women in Egypt

2013 ◽  
Vol 142 (1) ◽  
pp. 208-210 ◽  
Author(s):  
S. SHABAYEK ◽  
S. ABDALLA ◽  
A. MH. ABOUZEID
Keyword(s):  
High Frequency ◽  
Protein Gene ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Surface Proteins ◽  
Protein Encoding ◽  
Vaginal Swabs ◽  
Surface Protein Gene ◽  
Group B ◽  
Encoding Genes

SUMMARYGroup B streptococcus (GBS) is a leading cause of neonatal sepsis and meningitis. We determined the distribution of serotypes and surface protein encoding genes of GBS strains from pregnant and non-pregnant women in Egypt. Vaginal swabs from 364 women were screened by culture and 100 (27·4%) yielded GBS. Serotype V was the most predominant (33%), followed by serotypes II (17%), III (15%), Ia (14%), VI (12%), Ib (8%) and IV (1%). The most common surface protein genes were epsilon (27%), alp3 (26%), bca (18%), rib (16%) and alp2 (10%). Two isolates were negative for surface protein genes. The distribution of serotypes and surface proteins was similar to reports from other parts of the world but the relatively high frequency of serotype VI was a notable feature of the strains from women in Egypt.

scholarly journals Early protection against influenza by pandemic live attenuated influenza vaccines

2019 ◽  
Vol 19 (3) ◽  
pp. 37-46
Author(s):  
Andrey R. Rekstin ◽  
Julia A. Desheva ◽  
Irina V. Kiseleva ◽  
Irina N. Isakova-Sivak
Keyword(s):  
South Africa ◽  
Influenza Vaccine ◽  
Influenza A ◽  
Relative Sensitivity ◽  
Cytokine Response ◽  
Influenza Viruses ◽  
Surface Proteins ◽  
Cold Adapted

Live attenuated cold-adapted (ca) influenza vaccine (LAIV) is an effective tool for the control of influenza, most likely due to their ability to induce both humoral and cellular immune responses, easy application and relatively low manufacturing costs. Attenuated cold-adapted vaccine strains that have achieved a satisfactory balance between restricted replication and high immunogenicity are desirable. The immunogenicity of live attenuated vaccines may depend upon the interplay between its ability to induce pro-inflammatory cytokine responses and the relative sensitivity of the attenuated vaccine strain to an antiviral effect of these cytokines. To better understand the relationship between attenuation and induction of innate immunity as well as contribution of the early cytokine response to the relative immunogenicity of LAIVs, we have studied early protection induced by LAIV in vivo as well as early cytokine response in human cells macrophage origin in response to infection with vaccine strains or epidemic virus. The aim of this study was to investigate the early immune response and protective activity in female CBA mice intranasally immunized with cold-adapted influenza vaccine strains of different genome compositions of 5:3 or 6:2. For experimental infection pandemic influenza viruses A/South Africa/3626/13 (H1N1)pdm09 and A/New York/61/15 (H1N1)pdm09 were used to be administered to animals at a dose of 106 EID50 at day 3 after immunization (challenge infection). Although challenge viruses replicate at mice lungs at various, extend, on day 10 after immunization mice were protected from death from 60 up to 80%. Reassortants LAIV did not differ statistically on these levels. Study of the expression of IFN- and IFN- genes in human lung macrophage line cells THP-1 in vitro have shown that macrophages stimulated with vaccine strains with the genome formula 6:2 and 5:3, had a sufficient level of expression of these genes, comparable to that, as in infection with wild virus type A/South Africa/3626/13 (H1N1)pdm09. These data may indicate that surface proteins of influenza A virus are involved in the process of stimulation of the IFN- and IFN- genes.

scholarly journals Genomic Recombination Leading to Decreased Virulence of Group B Streptococcus in a Mouse Model of Adult Invasive Disease

Pathogens ◽  
2016 ◽  
Vol 5 (3) ◽  
pp. 54 ◽  
Author(s):  
Sarah Teatero ◽  
Paul Lemire ◽  
Ken Dewar ◽  
Jessica Wasserscheid ◽  
Cynthia Calzas ◽  
...  
Keyword(s):  
Mouse Model ◽  
Group B Streptococcus ◽  
Invasive Disease ◽  
Group B ◽  
Genomic Recombination
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