scholarly journals Association between antibodies against group B Streptococcus surface proteins and recto-vaginal colonisation during pregnancy

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Sonwabile Dzanibe ◽  
Gaurav Kwatra ◽  
Peter V. Adrian ◽  
Sheila Z. Kimaro-Mlacha ◽  
Clare L. Cutland ◽  
...  
Keyword(s):  
Group B Streptococcus ◽  
Surface Proteins ◽  
Group B

scholarly journals Identification of Major Outer Surface Proteins of Streptococcus agalactiae

2002 ◽  
Vol 70 (3) ◽  
pp. 1254-1259 ◽  
Author(s):  
Martin J. G. Hughes ◽  
Joanne C. Moore ◽  
Jonathan D. Lane ◽  
Rebecca Wilson ◽  
Philippa K. Pribul ◽  
...  
Keyword(s):  
Outer Surface ◽  
Streptococcus Agalactiae ◽  
Expression System ◽  
Group B Streptococcus ◽  
Phosphoglycerate Kinase ◽  
Peptide Sequencing ◽  
Surface Proteins ◽  
Outer Surface Proteins ◽  
Group B

ABSTRACT To identify the major outer surface proteins of Streptococcus agalactiae (group B streptococcus), a proteomic analysis was undertaken. An extract of the outer surface proteins was separated by two-dimensional electrophoresis. The visualized spots were identified through a combination of peptide sequencing and reverse genetic methodologies. Of the 30 major spots identified as S. agalactiae specific, 27 have been identified. Six of these proteins, previously unidentified in S. agalactiae, were sequenced and cloned. These were ornithine carbamoyltransferase, phosphoglycerate kinase, nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, purine nucleoside phosphorylase, enolase, and glucose-6-phosphate isomerase. Using a gram-positive expression system, we have overexpressed two of these proteins in an in vitro system. These recombinant, purified proteins were used to raise antisera. The identification of these proteins as residing on the outer surface was confirmed by the ability of the antisera to react against whole, live bacteria. Further, in a neonatal-animal model system, we demonstrate that some of these sera are protective against lethal doses of bacteria. These studies demonstrate the successful application of proteomics as a technique for identifying vaccine candidates.

Serotype and surface protein gene distribution of colonizing group B streptococcus in women in Egypt

2013 ◽  
Vol 142 (1) ◽  
pp. 208-210 ◽  
Author(s):  
S. SHABAYEK ◽  
S. ABDALLA ◽  
A. MH. ABOUZEID
Keyword(s):  
High Frequency ◽  
Protein Gene ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Surface Proteins ◽  
Protein Encoding ◽  
Vaginal Swabs ◽  
Surface Protein Gene ◽  
Group B ◽  
Encoding Genes

SUMMARYGroup B streptococcus (GBS) is a leading cause of neonatal sepsis and meningitis. We determined the distribution of serotypes and surface protein encoding genes of GBS strains from pregnant and non-pregnant women in Egypt. Vaginal swabs from 364 women were screened by culture and 100 (27·4%) yielded GBS. Serotype V was the most predominant (33%), followed by serotypes II (17%), III (15%), Ia (14%), VI (12%), Ib (8%) and IV (1%). The most common surface protein genes were epsilon (27%), alp3 (26%), bca (18%), rib (16%) and alp2 (10%). Two isolates were negative for surface protein genes. The distribution of serotypes and surface proteins was similar to reports from other parts of the world but the relatively high frequency of serotype VI was a notable feature of the strains from women in Egypt.

scholarly journals Evaluation in Mouse Model of Combined Virus-bacterial Vaccine Based on Attenuated Influenza A(H7N3) Virus and the Group B Streptococcus Recombinant Polypeptides

2016 ◽  
Vol 10 (1) ◽  
pp. 168-175 ◽  
Author(s):  
Yulia A. Desheva ◽  
Galina F. Leontieva ◽  
Tatiana A. Kramskaya ◽  
Tatiana A. Smolonogina ◽  
Kornelia B. Grabovskaya ◽  
...  
Keyword(s):  
Mouse Model ◽  
Influenza Vaccine ◽  
Influenza A ◽  
Group B Streptococcus ◽  
Surface Proteins ◽  
Wild Type Virus ◽  
Common Cause ◽  
Intranasal Immunization ◽  
Group B ◽  
Recombinant Polypeptides

Background:Secondary bacterial influenza complications are a common cause of excesses morbidity and mortality, which determines the need to develop means for specific prophylaxis. Group B streptococcal infection is especially common cause of pneumonia among children and the elderly with underlying conditions. Here we investigate in a mouse model the effects of combined intranasal immunization using live attenuated influenza vaccine and recombinant polypeptides based on group BStreptococcussurface proteins.Methods:Groups of outbred mice received two doses of the following preparations: 1) the reassortant A/17/Mallard/Netherlands/00/95 (H7N3) influenza virus; 2) a mixture of P6, ScaAB, ScpB1 and Stv recombinant GBS proteins (20 µg total); 3) the A(H7N3) influenza vaccine pooled with the four bacterial peptide preparation; 4) control animals were treated with PBS.Results:Intranasal vaccination using LAIV in combination with GBS polypeptides provided advantageous protection against infections with homologous A/Mallard/Netherlands/12/00 (H7N3) wild type virus or heterologous A/Puerto Rico/8/34 (H1N1) followed by serotype II GBS infection. Also, combined vaccination improved bacterial clearance from the lungs of mice.Conclusion:Intranasal immunization with LAIV+GBSV was safe and enabled to induce the antibody response to each of vaccine components. Thus, the combined vaccine increased the protective effect against influenza and its bacterial complications in mice compared to LAIV-only.

Group B Streptococcus surface proteins as major determinants for meningeal tropism

2012 ◽  
Vol 15 (1) ◽  
pp. 44-49 ◽  
Author(s):  
Asmaa Tazi ◽  
Samuel Bellais ◽  
Isabelle Tardieux ◽  
Shaynoor Dramsi ◽  
Patrick Trieu-Cuot ◽  
...  
Keyword(s):  
Group B Streptococcus ◽  
Surface Proteins ◽  
Group B

scholarly journals Group B Streptococcal Surface Proteins as Targets for Protective Antibodies: Identification of Two Novel Proteins in Strains of Serotype V

1999 ◽  
Vol 67 (12) ◽  
pp. 6350-6357 ◽  
Author(s):  
Thomas Areschoug ◽  
Margaretha Stålhammar-Carlemalm ◽  
Charlotte Larsson ◽  
Gunnar Lindahl
Keyword(s):  
Vaccine Development ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Cross Reactivity ◽  
Active Immunization ◽  
Surface Proteins ◽  
Protective Antibodies ◽  
Group B ◽  
Different Strains ◽  
Type V

ABSTRACT Strains of group B streptococcus (GBS) express surface proteins that confer protective immunity. In particular, most strains of the four classical capsular serotypes (Ia, Ib, II, and III) express either of the Rib and α proteins, two members of the same protein family. Here, we report a study of surface proteins expressed by strains of serotype V, which has recently emerged as an important serotype among GBS strains causing serious disease. Two novel GBS proteins were identified, purified, and characterized. One of these proteins, designated Fbs, was immunologically unrelated to other GBS surface proteins. This ∼110-kDa protein was found in 15 of 49 (31%) type V isolates but in few strains of other serotypes. The Fbs proteins expressed by different strains showed limited variation in size. The most common surface protein among type V strains, found in 29 of 49 (59%) isolates, was designated Rib-like, since it cross-reacted with Rib but was not immunologically identical to Rib. Characterization of this Rib-like protein showed that the N-terminal sequence (12 residues) was identical to that of α, although these two proteins lacked cross-reactivity. The biochemical and immunological properties of the Rib-like GBS protein indicate that it is closely related to the R28 protein of Streptococcus pyogenes. Importantly, passive and active immunization experiments with mice showed that the Fbs and Rib-like proteins are targets for protective antibodies. These two proteins are therefore of interest for analysis of pathogenic mechanisms and for vaccine development.

scholarly journals High prevalence of group B streptococcus ST17 hypervirulent clone among non-pregnant patients from a Hungarian venereology clinic

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Szilvia Kardos ◽  
Adrienn Tóthpál ◽  
Krisztina Laub ◽  
Katalin Kristóf ◽  
Eszter Ostorházi ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
Clinical Symptoms ◽  
Strong Association ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Surface Proteins ◽  
High Prevalence ◽  
Intrapartum Antibiotic ◽  
Group B ◽  
Open Question

Abstract Background Although Streptococcus agalactiae is the leading causative agent of neonatal sepsis and meningitis, recently it is increasingly isolated from non-pregnant adults. The relation between its presence in the genitourinary tract and manifested clinical symptoms of STD patients remains an open question. In this study, a complex epidemiological investigation of GBS isolates from a venerology clinic was performed. Methods Ninety-six GBS isolates were serotyped and their genetic relatedness determined by PFGE. MLST was also performed for a subset of 20 isolates. The antibiotic susceptibility was tested with agar dilution. Surface proteins and the ST-17 hypervirulent clone was detected by PCR. Results The serotype prevalence was the following: V (29.2%), III (27.1%), Ia (22.9%), IV (10.4%), II (5.2%) and Ib (4.2%). A strong association was demonstrated between surface protein genes and serotypes. All isolates were fully susceptible to penicillin, but erythromycin and clindamycin resistance was high (41.7 and 35.4%, respectively), and 8 phenotypically macrolide sensitive isolates carried the ermB gene. 21.9% of all strains belonged to the hypervirulent ST17 clone, most being of serotype III and all were rib +. We found a few serotype IV isolates belonging to several STs and one serotype V/ST110 strain, containing a 44-bp deletion in the atr allele. Conclusions The presence of silent ermB genes is of worry, as their expression upon macrolide exposure could lead to unforeseen therapeutic failure, while clindamycin is used for intrapartum antibiotic prophylaxis, in case of penicillin allergy. The other alarming result is the high prevalence of ST17 among these strains from STD patients, who could be sources of further infections. This is the first report from Hungary providing both serotyping and genotyping data of GBS isolates. These results could be helpful for vaccine production as the major vaccine candidates are capsular antigens or surface proteins.

scholarly journals Genetic characterization and diversity of Streptococcus agalactiae isolates with macrolide resistance

2010 ◽  
Vol 59 (7) ◽  
pp. 780-786 ◽  
Author(s):  
Monika Brzychczy-Włoch ◽  
Tomasz Gosiewski ◽  
Małgorzata Bodaszewska ◽  
Wojciech Pabian ◽  
Małgorzata Bulanda ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
Macrolide Resistance ◽  
Surface Proteins ◽  
Erythromycin Resistance ◽  
High Genetic Diversity ◽  
Genes Encoding ◽  
Resistant Strains ◽  
Group B

Macrolide resistance in 169 Streptococcus agalactiae [group B streptococcus (GBS)] isolates originating from pregnant carriers was investigated. Using multiplex PCR the presence of genes encoding erythromycin resistance and capsular polysaccharides, as well as surface proteins, was determined. Random amplification of polymorphic DNA (RAPD) and PFGE were used to characterize specific clones among the isolates. In the examined population of women, erythromycin-resistant strains were found in 4.5 % of patients, whereas clindamycin-resistant strains were found in 3 % of patients, which was 16 % of strains resistant to erythromycin and 10 % of strains resistant to clindamycin among GBS isolates, respectively. Among the isolates, the largest percentage was represented by the constitutive macrolide–lincosamide–streptogramin B (cMLSB) phenotype (63 %), then the inductive macrolide–lincosamide–streptogramin B (iMLSB) phenotype (26 %) and the macrolide resistance (M) phenotype (11 %). The ermB gene was indicated in all isolates with the cMLSB phenotype and V serotype, whereas mefA/mefE genes were found in isolates with the M phenotype and Ia serotype. Among resistance isolates, serotype V was predominant (67 %), followed by serotypes II (15 %), Ia (11 %) and III (7 %). The most common surface protein encoding genes were alp3 (70 %), then rib (11 %), epsilon (7.5 %), bca (7.5 %) and alp2 (4 %). A statistically significant relationship between macrolide resistance, serotype V and the alp3 gene was demonstrated. PFGE, in comparison to the RAPD method, gave better genetic discrimination of GBS isolates. A relatively high genetic diversity among investigated strains was shown. In addition, the largest genetic homogeneity was found in serotype V.

scholarly journals Identification of novel cps locus polymorphisms in nontypable group B Streptococcus

2006 ◽  
Vol 55 (6) ◽  
pp. 775-783 ◽  
Author(s):  
Srinivas V. Ramaswamy ◽  
Patricia Ferrieri ◽  
Lawrence C. Madoff ◽  
Aurea E. Flores ◽  
Nikhil Kumar ◽  
...  
Keyword(s):  
Capsular Polysaccharide ◽  
Group B Streptococcus ◽  
Surface Protein ◽  
The Elderly ◽  
Surface Proteins ◽  
Pcr Analysis ◽  
Genetic Profile ◽  
Gene Encoding ◽  
Group B ◽  
Type V

Group B Streptococcus (GBS) is an important pathogen responsible for a variety of diseases in newborns and the elderly. A clinical GBS isolate is considered nontypable (NT) when serological methods fail to identify it as one of nine known GBS serotypes. Eight clinical isolates (designated A1–A4, B1–B4) showed PFGE profiles similar to that of a GBS serotype V strain expressing R1, R4 surface proteins. These unique isolates were further characterized by immunologic and genetic methods. Rabbit sera to isolates A1 and A2 reacted weakly with concentrated HCl extracts of A1–A4 isolates, but not with those of B1–B4 isolates. In addition, a type V capsular polysaccharide (CPS) inhibition ELISA revealed that cell wall extracts from isolates A1–A4, but not from B1–B4, expressed low but measurable amounts of type V CPS. Molecular serotyping with PCR analysis showed that all eight isolates contained a type V-specific CPS gene (cpsO) and harboured the gene encoding the surface protein Alp3. Multilocus sequence typing identified isolate A1 as belonging to a new sequence type (ST) designated ST-173, whereas the other seven isolates keyed to ST-1. Sequencing of the 18 genes (17 736 bp) in the cps locus showed that each NT isolate harboured one to three unique polymorphisms, and also identified an IS1381 element in cpsE of the B4 isolate. Collectively, genetic and immunologic analyses revealed that these NT isolates expressing R1, R4 proteins have a genetic profile consistent with that of type V, an emergent, antigenically diverse and increasingly prevalent GBS serotype.

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