Comparative study on polyphenols content and antioxidant activity of three sweet pepper varieties (Capsicum annuum L.)

Background: The genus Capsicum contains various sweet and hot pepper varieties, including Capsicum annum L. The various species in this genus are used as herbs, vegetables, or medicines, and recent studies have shown that they are a rich source of bioactive compounds as well. Objective: In this study, our objective was to evaluate the antioxidant activity as well as the content of phenols (TPC), the content of flavonoids (TFC) and total condensed tannins (TCT) of ethanolic extracts of the fresh and dried sweet pepper Capsicum annuum L. Methods: The antioxidant activities of the extracts were examined using different biochemical assays namely diphenylpicrylhydrazyl (DPPH), total antioxidant capacity (TAC) and ferric reducing power (FRAP). The total phenolic contents (TPC) were determined spectrophotometrically according to the Folin-Ciocalteu colorimetric method. Total flavonoid content was measured by the aluminum chloride colorimetric assay. High-performance liquid chromatography (HPLC) has been using to identify the phenolic compounds in ethanolic extracts. Results: The obtained results showed the presence of considerable amounts of total phenolic (757.3±3.5 mgGAE/100g), flavonoid (482.66±1.5 mgQE/100g) and Condensed Tannins (15.97±0.5 mg GAE/100g) content in dry red pepper extract. The use of HPLC has allowed the identification of Gallic Acid, Ascorbic Acid, Chlorogenic Acid, Caffeic Acid, Quercetin, Vanillin, and Rutin in pepper ethanolic extracts. Green and yellow dry peppers contain significant amounts of gallic acid exceeding (134.0 µg /mg). The fresh yellow and red peppers also contained significant amounts of Quercetin exceeding (109.3 µg/mg), the dry red pepper has the strongest antioxidant activity. Conclusion: In conclusion, these findings can be regarded as very promising and justify further study including the identification of antioxidant components in pepper extracts. Our work constitutes a first step in the study of fresh and dry sweet pepper as a promising source of natural antioxidants.

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Effect of drying in antioxidant activity and changes of ascorbic acid and colour by different drying and storage in Korean red pepper (Capsicum annuum, L.)

International Journal of Food Science & Technology ◽

10.1111/j.1365-2621.2006.01349.x ◽

2006 ◽

Vol 41 (s1) ◽

pp. 90-95 ◽

Cited By ~ 30

Author(s):

Suna Kim ◽

Ki Won Lee ◽

Jaebok Park ◽

Hyong J. Lee ◽

In K. Hwang

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Capsicum Annuum ◽

Red Pepper ◽

Capsicum Annuum L ◽

And Storage

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Phenolic Composition and Antioxidant Activity of Leaves of Strawberry Tree (Arbutus unedo L.) Populations from Morocco

Phytothérapie ◽

10.3166/phyto-2021-0296 ◽

2021 ◽

Author(s):

Z. Ait lhaj ◽

K. Taghzouti ◽

A. Bouyahya ◽

G. Diria ◽

K. Bakhy ◽

...

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic ◽

Phenolic Composition ◽

Leaf Extracts ◽

Arbutus Unedo ◽

Scavenging Effect ◽

Promising Source

This study was aimed at determining the phenolic composition and the antioxidant activity of leaves of Arbutus unedo populations belonging to seven Moroccan locations. Antioxidant activity is evaluated using two methods: scavenging effect on DPPH and scavenging effect on ABTS radicals. The Arbutus unedo leaf extracts displayed average values of 61.95 mg GAE, 3.60 mg QE, and 0.15 mg CE per g of dry weight (DW) in polyphenols, flavonoids, and tannins contents, respectively. All extracts exhibited a strong antioxidant effect on both assays, presenting mean IC50 values of 4.22 in DPPH assay and 4.86 μg/ml in ABTS assay. Significant variability (P < 0.0001) was showed among surveyed populations; samples from the Chefchaouen location (CH) was revealed the highest total phenolic content (107.67 mg GAE/g DW) and the strongest antioxidant activity (IC50 values of 0.80 and 1.56 μg/ml, in DPPH and ABTS tests, respectively). The phenolic profile achieved by HPLC-DAD-ESI/MS was dominated by gallic acid, Gallic acid glucose derivative, and Galloylquinic acid derivative. A significant negative correlation (P < 0.05) was observed between the total phenolic content and the antioxidant activity. These results provide that Arbutus unedo leaves should be considered as a promising source of natural antioxidants, and as ingredients in complimentary food and nutraceutical products.

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Antioxidant properties of species from the Brazilian cerrado by different assays

Revista Brasileira de Plantas Medicinais ◽

10.1590/s1516-05722013000400008 ◽

2013 ◽

Vol 15 (4) ◽

pp. 520-528 ◽

Cited By ~ 6

Author(s):

K.S. Farias ◽

T.S.N. Santos ◽

M.R.A.B. Paiva ◽

S.M.L. Almeida ◽

P.T. Guedes ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Reducing Power ◽

Total Phenolic ◽

Brazilian Cerrado ◽

Medicinal Plant Extracts ◽

Ethanolic Extracts ◽

Total Antioxidant ◽

Promising Source

The purpose of this study was to screen the antioxidant activity of medicinal plant extracts from the Brazilian cerrado, through other methods than the total phenolic content and its correlation with the antioxidant activity. Ethanolic extracts of ten species were evaluated through three antioxidant assays, in vitro, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), total antioxidant activity and reducing power; and by using the Folin-Ciocalteu method the total phenolic content was determined. Ethanolic extracts of Stryphnodendron obovatum, Cecropia pachystachya and Duguetia furfuraceae showed strong antioxidant activity (IC50<5 µg mL-1) in the DPPH free radical scavenging assay; the species Vernonia phosphorea, Hymenaea stignocarpa and Jacaranda ulei may also be highlighted. These results were confirmed in the assays of total antioxidant capacity and reducing power. The extracts of S. obovatum and V. phosphorea showed an abundant phenolic content; therefore, the phenolic content may play a role in the antioxidant activity. These two species, traditionally used in Brazil, showed great power in these assay systems and may be a promising source for the development of natural antioxidants and future candidates for phytochemical and pharmacological studies in related diseases.

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Determination of Phenolic Compounds in Red Sweet Pepper (Capsicum annuum L.) using a Modified QuEChERS Method and UHPLC-MS/MS Analysis and Its Relation to Antioxidant Activity

Journal of the Brazilian Chemical Society ◽

10.21577/0103-5053.20190018 ◽

2019 ◽

Cited By ~ 5

Author(s):

Carina Rodrigues ◽

Antonio Nicácio ◽

Isabel Jardim ◽

Jesuí Visentainer ◽

Liane Maldaner

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Capsicum Annuum ◽

Sweet Pepper ◽

Quechers Method ◽

Capsicum Annuum L ◽

Modified Quechers ◽

Ms Analysis

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Phytochemical Profile of Capsicum annuum L. cv Senise, Incorporation into Liposomes, and Evaluation of Cellular Antioxidant Activity

Antioxidants ◽

10.3390/antiox9050428 ◽

2020 ◽

Vol 9 (5) ◽

pp. 428

Author(s):

Chiara Sinisgalli ◽

Immacolata Faraone ◽

Antonio Vassallo ◽

Carla Caddeo ◽

Faustino Bisaccia ◽

...

Keyword(s):

Antioxidant Activity ◽

Capsicum Annuum ◽

Ethanol Extract ◽

Sweet Pepper ◽

Capsicum Annuum L ◽

Negative Surface ◽

Negative Surface Charge ◽

Endogenous Antioxidants ◽

Stressed Cells ◽

Vegetables And Fruits

Overproduction of oxidants in the human body is responsible for oxidative stress, which is associated with several diseases. High intake of vegetables and fruits can reduce the risk of chronic diseases, as they are sources of bioactive compounds capable of contrasting the free radical effects involved in cancer, obesity, diabetes, and neurodegenerative and cardiovascular diseases. Capsicum annuum L. cv Senise is a sweet pepper that is grown in the Basilicata region (Italy). It is an important source of polyphenols, carotenoids, and capsinoids and can play a key role in human health. In this study, an ethanol extract was obtained from C. annuum dried peppers and the analysis of the phytochemical composition was performed by LC-ESI/LTQ Orbitrap/MS. The extract was incorporated into liposomes, which showed small size (~80 nm), good homogeneity, negative surface charge, and good stability in storage. The biological activity of the extract was evaluated in the human hepatoma (HepG2) cell line, used as model cells. The extract showed no cytotoxic activity and reduced the intracellular reactive oxygen species (ROS) level in stressed cells. The antioxidant activity was further improved when the extract was loaded into liposomes. Moreover, the extract promoted the expression of endogenous antioxidants, such as catalase, superoxide dismutase, and glutathione peroxidase through the Nrf-2 pathway evaluated by RT-PCR.

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Effect of Pre-Harvest Organic Cytokinin Application on the Post-Harvest Physiology of Pepper (Capsicum annuum L.)

Sustainability ◽

10.3390/su13158258 ◽

2021 ◽

Vol 13 (15) ◽

pp. 8258

Author(s):

Seyda Cavusoglu ◽

Suat Sensoy ◽

Arzu Karatas ◽

Onur Tekin ◽

Firat Islek ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Capsicum Annuum ◽

Respiration Rate ◽

Control Sample ◽

Significant Loss ◽

Enzyme Analysis ◽

Total Phenolic ◽

Positive Effects ◽

Capsicum Annuum L

Peppers are cultivated in almost all provinces in Turkey, making up approximately 7% of the world’s production. In this study, the effects of pre-harvest cytokinin application on the postharvest physiology of pepper (Capsicum annuum L.) fruits (cv. Akra and Melek F1) grown in ecological conditions in Turkey (Van, eastern Turkey) were investigated. During the growing period, organic cytokinin(nytrozine) at two concentrations (50 and 100 ppm doses) was applied by spraying. Peppers were harvested in green maturity and then stored at 12 °C for 28 days. Changes in color, respiration rate, exogenous ethylene amount, antioxidative enzyme analysis (SOD, CAT and APX), lipid peroxidation, total phenolic content (TP) and antioxidant activity (AA) were determined just after the harvest and then at the 7th, 14th, 24th and 28th days. L* values for both varieties and the control sample had fluctuations during storage, and they slightly decreased after 28 days, except for the end value of cv. Melek F1 with a 50 ppm addition. In terms of respiration rate values, the difference between applications was found to be statistically significant, except for the 14th and 24th days of storage, for cv. Akra F1; and the 0th and 28th days for cv. Melek F1, respectively. It was observed that cytokinin can delay aging in peppers of both cultivars stored for 28 days. As a result, as the used cytokinin has an organic origin, is not synthetic and does not have a negative effect on human health, it can be used safely and without a significant loss of quality characteristics for peppers. These applications have positive effects on in-package gas composition, antioxidative enzymes, lipid peroxidation, total phenolics and total antioxidant activity.

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