Biodegradation Study of Potato Starch-based Bioplastic

2021 ◽  
Vol 01 ◽  
Author(s):  
Rajen Kundu ◽  
Priyanka Payala
Keyword(s):  
Natural Environment ◽  
Potato Starch ◽  
Environmental Issues ◽  
Degradable Polymer ◽  
Solid Media ◽  
Degrading Bacteria ◽  
Biodegradation Study ◽  
Bacteria Culture ◽  
Cultured Bacteria

Background: Plastics are indispensable for our society. The extensive use of petroleum-based plastic and dumping of the same in soil and water body greatly affects our environment and biodiversity. However, biodegradable plastics can reduce the volume of waste in packaging materials. Therefore, biomass-derived polymers are promising alternatives of the petroleum-based non-degradable polymer to address the environmental issues. Objective: A large number of reports on the synthesis and characterization of starch-based bioplastic are available in the literature. However, a detailed biodegradation study of the starch-based bioplastic is rarely reported. We have prepared potato starch-based bioplastic with the combination of various plasticizers (glycerol, sorbitol, and xylitol) through hydrogel formation and carried out their biodegradation study. Method: Present study investigated the biodegradation of potato starch-based bioplastic in the natural environment, in cultured bacteria, and with fungal α-amylase. Results: Starch-based plastic is completely degraded in the natural environment within two months. Bacteria culture in solid media resulted in various types of bacterial colonies. Among the various bacterial colonies, the white circular colony was the major bacteria that degrade starch-based plastic. Furthermore, we screened the starch-based plastic degrading bacteria and isolated the pure culture through the streak plate method. Conclusion: n presence of cultured bacteria and with fungal α-amylase, starch-based plastic is completely degraded within 96h and 48h respectively.

scholarly journals Multi-omics approach reveals new insights into the gut microbiome of Galleria mellonella (Lepidoptera:Pyralidae) exposed to polyethylene diet

2021 ◽  
Author(s):  
Samuel Latour ◽  
Gregoire Noel ◽  
Laurent Serteyn ◽  
Abdoul Razack Sare ◽  
Sebastien Massart ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Gut Microbiome ◽  
Galleria Mellonella ◽  
Community Change ◽  
Sequence Variant ◽  
Plastic Pollution ◽  
Degrading Bacteria ◽  
Biodegradation Study

The current plastic pollution throughout the world implies a crucial optimization of its (bio)degradation processes. In order to identify plastic degrading bacteria and associated enzymes, the gut microbiota of insects has raised interest. Some entomological models such as Tenebrio molitor (L. 1758), Plodia interpunctella (Hubner 1813) or Galleria mellonella (L. 1758) have the ability to ingest and degrade polyethylene. Then, it is promising to identify the composition and the role of the gut microbiota in this process. This study takes part in this issue by investigating G. mellonella as a biological model feeding with a polyethylene diet. Gut microbiome samples were processed by high throughput 16S rRNA sequencing, and Enterococcaceae and Oxalobacteraceae were found to be the major bacterial families. At low polyethylene dose, we detect no bacterial community change and no amplicon sequence variant associated with the polyethylene diet suggesting microbiome resilience. The functional analysis of insects gut content was promising for the identification of plastic degrading enzymes such as the phenylacetaldehyde dehydrogenase which participate in styrene degradation. This study allows a better characterization of the gut microbiota of G. mellonella and provides a basis for the further biodegradation study of polyethylene based on the microorganism valorization from insect guts.

scholarly journals Norwegian Soils and Waters Contain Mesophilic, Plastic-Degrading Bacteria

2021 ◽  
Vol 9 (1) ◽  
pp. 94
Author(s):  
Colin Charnock
Keyword(s):  
Environmental Issues ◽  
Plastic Pollution ◽  
Streptomyces Sp ◽  
Potential Source ◽  
Degrading Bacteria ◽  
Natural Remediation ◽  
Polyphasic Characterization ◽  
Rhodococcus Sp ◽  
Microbial Attack

Plastic pollution has become one of the most critical environmental issues, as rapidly increasing production, compounded by persistence of plastic wastes in the environment, are outpacing efforts to keep ecosystems plastic-free. A switch to plastics more amenable to microbial attack is one of several possible responses. Against this background, the current study describes the isolation, enumeration and polyphasic characterization of plastic-degrading bacteria present in Norwegian terrestrial and aquatic habits. It shows that these bacteria are present in relatively high numbers, and that plastic-degrading capabilities are found in several taxa, most especially Streptomyces. Some isolates wereable to degrade several plastics. Notably, a Rhodococcus sp. and a Streptomyces sp. degraded, respectively, four and six of the eight plastics investigated and a number of other polymers relevant for plastic blends. The paper also has a methodological aspect, presenting various approaches for assaying plastic-degrading properties and a PCR/sequencing-based approach for the identification of potential polyethylene terephthalate-degrading genes. A candidate gene was detected in several Streptomyces isolates. The study shows that Norwegian environments are a rich source of bacteria with the ability to degrade bioplastics possibly representing a natural remediation capacity, as well as a potential source of useful enzymes.

Enrichment and Characterization of Hydrocarbon Degrading Bacteria from Various Oil-Contaminated Sites in Pakistan

2021 ◽  
pp. 1-11
Author(s):  
Amna Aqeel ◽  
Zahid Hussain ◽  
Qurat-Ul-Ain Aqeel ◽  
Javaria Zafar ◽  
Naureen Ehsan ◽  
...  
Keyword(s):  
Contaminated Sites ◽  
Degrading Bacteria

Isolation of cyanide‐degrading bacteria and molecular characterization of its cyanide‐degrading nitrilase

2020 ◽  
Author(s):  
Ayane Terada ◽  
Daisuke Komatsu ◽  
Takahiro Ogawa ◽  
Darin Flamandita ◽  
Muhamad Sahlan ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Degrading Bacteria

The Environmental Protection Consciousness and Countermeasures

2014 ◽  
Vol 962-965 ◽  
pp. 1509-1512
Author(s):  
Lin Liu ◽  
Pin Lv
Keyword(s):  
Economic Development ◽  
Environmental Protection ◽  
Natural Environment ◽  
Human Activities ◽  
Environmental Issues ◽  
The World ◽  
The Future ◽  
Environmental Concepts ◽  
Environment Sustainability ◽  
Development And Environment

There are various signs indicating that the Earth's natural environment is changing toward unfavorable direction for species, which is highly suspected to be connected with human activities. In the last century, people all over the world have realized the severity of environmental issues. In the long history, Chinese ancient had already development good rules and methods to reach balance between economic development and environment sustainability. This paper will discuss how environmental concepts forms and which methods could be applied in the future.

Isolation and characterization of 3-nitrophenol-degrading bacteria associated with rhizosphere of Spirodela polyrrhiza

2012 ◽  
Vol 19 (5) ◽  
pp. 1852-1858 ◽  
Author(s):  
Risky Ayu Kristanti ◽  
Masahiro Kanbe ◽  
Tony Hadibarata ◽  
Tadashi Toyama ◽  
Yasuhiro Tanaka ◽  
...  
Keyword(s):  
Spirodela Polyrrhiza ◽  
Isolation And Characterization ◽  
Degrading Bacteria

scholarly journals Isolation and characterization of organic matter-degrading bacteria from coking wastewater treatment plant

2018 ◽  
Vol 78 (7) ◽  
pp. 1517-1524 ◽  
Author(s):  
Riqiang Li ◽  
Jianxing Wang ◽  
Hongjiao Li
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Removal Rate ◽  
Treatment Plant ◽  
Coking Wastewater ◽  
Naphthalene Degradation ◽  
Isolation And Characterization ◽  
Degrading Bacteria

Abstract As a step toward bioaugmentation of coking wastewater treatment 45 bacteria strains were isolated from the activated sludge of a coking wastewater treatment plant (WWTP). Three strains identified as Bacillus cereus, Pseudomonas synxantha, and Pseudomonas pseudoaligenes exhibited high dehydrogenase activity which indicates a strong ability to degrade organic matter. Subsequently all three strains showed high naphthalene degradation abilities. Naphthalene is a refractory compound often found in coking wastewater. For B. cereus and P. synxantha the maximum naphthalene removal rates were 60.4% and 79.8%, respectively, at an initial naphthalene concentration of 80 mg/L, temperature of 30 °C, pH of 7, a bacteria concentration of 15% (V/V), and shaking speed of 160 r/min. For P. pseudoaligenes, the maximum naphthalene removal rate was 77.4% under similar conditions but at 35 °C.

Sign in / Sign up

Export Citation Format

Share Document