scholarly journals Isolation and characterization of Arbuscular Mycorrhizal (AM) fungi from the rhizospheric soil of some medicinal plants

2019 ◽  
Vol 7 (12) ◽  
pp. 2459-2467
Author(s):  
Dhritiman Chanda ◽  
Madhumita Dey
Keyword(s):  
Medicinal Plants ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Andrographis Paniculata ◽  
Ocimum Sanctum ◽  
Rhizospheric Soil ◽  
Human Welfare ◽  
Adhatoda Vasica ◽  
Isolation And Characterization ◽  
Spore Population

The present investigation was carried out to estimate the colony forming units of indigenous fungi and to study the diversity and Arbuscular mycorrhizal (AM) of some medicinal plants. The study was also aimed to estimate and isolate the Arbuscular mycorrhizal (AM) spore population from the rhizospheric soil of some important medicinal plants from the Botanical garden of College campus. All the selected medicinal plants i.e., Mentha sp. Ocimum sanctum, Bryophyllum sp. Adhatoda vasica, and Andrographis paniculata showed a very good diversity of rhizospheric soil fungi. The highest number of Colony forming unit (c.f.u) was estimated in Mentha sp (162/10-1) followed by Bryophyllum sp. (156/10-1), Andrographis paniculata (91/10-1), Adhatoda vasica (72/10-1) and Ocimum sanctum (68/10-1). Arbuscular mycorrhizal (AM) spore population was also estimated in all the medicinal plants. The higest number of AM spores was estimated in Andrographis paniculata (121/50gm-1) followed by Ocimum sanctum (112/50gm-1), Adhatoda vasica (94/50gm-1), Bryophyllum sp.(89/50gm-1) and Mentha sp (75/ 50gm-1). The rhizospheric soil fungal diversity including mycorrhizal fungi (Glomus sp.) also was found to be beneficial for increasing the photosynthetic activity and uptake of phosphorus in medicinal plants. Thus, the use of AM inoculum can increase the yield of medicinal plants along with their secondary metabolites to utilize on a sustainable basis as a traditional and herbal medicine for the human welfare.

scholarly journals Response of Some Medicinal Plants to Vesicular Arbuscular Mycorrhizal Inoculations

2009 ◽  
Vol 1 (2) ◽  
pp. 381-386 ◽  
Author(s):  
B. Karthikeyan ◽  
M. M. Joe ◽  
A. J. Cheruth
Keyword(s):  
Medicinal Plants ◽  
Catharanthus Roseus ◽  
Mycorrhizal Fungi ◽  
Dry Matter ◽  
Arbuscular Mycorrhizal ◽  
Ocimum Sanctum ◽  
Chlorophyll Contents ◽  
Matter Production ◽  
Cymbopogon Flexuosus ◽  
Vesicular Arbuscular

An investigation has been made about the response of vesicular arbuscular mycorrhizal fungi of G. fasciculatum on some medicinal plants. Medicinal plants species selected for the study are Ocimum sanctum, Catharanthus roseus, Coleus forskholii and Cymbopogon flexuosus. The percentage of VAM association is 85 and the intensity of formation of vesicles and arbuscules  are 70% and 30%, respectively in VAM inoculated C. roseus plants. The total dry matter production (shoot and root dry wt), protein and total chlorophyll contents are seen to increase in VAM inoculated plants. The percentage of increase is more in C. roseus, followed by Cymbopogon flexosus when compared to control plants. Keywords: Ocimum sanctum, Coleus forskholii, Cymbopogon flexosus, Catharanthus roseus, VAM fungi.© 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.DOI: 10.3329/jsr.v1i2.1675 

Soil pH-induced changes in root colonization, diversity, and reproduction of symbiotic arbuscular mycorrhizal fungi from healthy and declining maple forests

2000 ◽  
Vol 30 (10) ◽  
pp. 1543-1554 ◽  
Author(s):  
Andrew P Coughlan ◽  
Yolande Dalpé ◽  
Line Lapointe ◽  
Yves Piché
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Acer Saccharum ◽  
Sugar Maple ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal ◽  
Taxonomic Diversity ◽  
Arbuscular Mycorrhizas ◽  
Fungal Community Composition ◽  
Spore Population

Acer saccharum Marsh. (sugar maple) is one of only few arbuscular mycorrhizal trees to form extensive stands in northern temperate biomes. Recent maple decline could result from altered intensity and quality of root colonization by associated mycobionts or possible shifts in symbiotic fungal community composition following environmental stresses. In this study the effects on arbuscular mycorrhizal fungi of soil acidification, one of several proposed causal stresses underlying forest decline, and remedial liming were investigated under glasshouse conditions. Acer saccharum seedlings were grown in unsterilized, pH altered, forest soils from healthy and declining maple stands. Over a range of treatment pHs normally tolerated by A. saccharum, fungal populations and responses to pH changes differed between the two soils. The declining site with more acidic soil had an initially larger spore population but lower taxonomic diversity than the healthy site. However, liming stimulated sporulation of several taxa initially apparently absent from the declining site spore population. The quantity of colonization generally increased with pH for both sites. Five Glomus taxa and Scutellospora calospora (Nicol. & Gerd.) Walker & Sanders are added to the list of fungi known to form arbuscular mycorrhizas with A. saccharum, and the known range of Acaulospora cavernata Blaszkowski is extended from Poland to eastern North America.

scholarly journals A review of the interaction of medicinal plants and arbuscular mycorrhizal fungi in the rhizosphere

2021 ◽  
Vol 49 (3) ◽  
pp. 12454
Author(s):  
Rui-Ting SUN ◽  
Ze-Zhi ZHANG ◽  
Nong ZHOU ◽  
A.K. SRIVASTAVA ◽  
Kamil KUČA ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Mycorrhizal Fungi ◽  
Growth Promotion ◽  
Clinical Importance ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Arbuscular Mycorrhizas ◽  
High Concentration ◽  
Clinical Drugs

Medicinal plants are well known to have the advantages of high concentration of medicinal ingredients having clinical importance, curative value, small toxic and side effects. Important compounds viz., paclitaxel, camptothecin, and vincristine have been developed from medicinal plants as first-line of clinical drugs, leading to their consistently increasing demand globally. However, the destruction of natural environment due to excessive mining threatened such resources jeopardizing the successful growing of medicinal plants. A group of beneficial arbuscular mycorrhizal (AM) fungi is known to exist in the rhizosphere of medicinal plants, which can establish a reciprocal symbiosis with their roots, namely arbuscular mycorrhizas. These AM fungi are pivotal in the habitat adaptation of medicinal plants. Studies have demonstrated that AM fungi aided in growth promotion and nutrient absorption of medicinal plants, thereby, accelerating the accumulation of medicinal ingredients and aiding resistance against abiotic stresses such as drought, low temperature, and salinity. An AM-like fungus Piriformospora indica is known to be cultured in vitro without roots, later showed analogous effects of AM fungi on medicinal plants. These fungi provide new mechanistic pathways towards the artificial cultivation of medicinal plants loaded with ingredients in huge demand in international market. This review provides an overview of the diversity of AM fungi inhabiting the rhizosphere of medicinal plants, and analyzes the functioning of AM fungi and P. indica, coupled with future lines of research.

Clary sage cultivation and mycorrhizal inoculation influence rhizosphere fungal community structure over time in a trace-element polluted site

2021 ◽  
Author(s):  
Robin Raveau ◽  
Joël Fontaine ◽  
Mohamed Hijri ◽  
Anissa Lounès Hadj-Sahraoui
Keyword(s):  
Community Structure ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Fungal Communities ◽  
Rhizospheric Soil ◽  
Polluted Soils ◽  
Salvia Sclarea ◽  
Fungal Microbiota ◽  
Amf Communities ◽  
Over Time

<p>Fungal community in the soil plays a central role in natural systems and agroecosystems, therefore it attracted much research interests. However, the fungal microbiota of aromatic plants, such as Salvia sclarea L., especially in trace-element (TE) polluted conditions and within the framework of phytomanagement approaches, remains unexplored. The presence of high concentrations of TE in the soil is likely to negatively affect not only microbial diversity and community structures, but also plant establishment and growth. The objective of this study is to investigate the soil fungal and arbuscular mycorrhizal fungi (AMF) community structure and their changes over time in TE-polluted soils in the vicinity of a former lead smelter and under the cultivation of clary sage. We used Illumina MiSeq amplicon sequencing to evaluate the effects of in situ clary sage cultivation during two successive years, combined or not with an exogenous AMF inoculation, on the rhizospheric soil and root fungal communities. We obtained 1239 and 569 fungal amplicon sequence variants (ASV) respectively in the rhizospheric soil and roots of S. sclarea in TE-polluted conditions. Remarkably, 69 AMF species were detected in our experimental site, belonging to 12 AMF genera. Besides, the inoculation treatment significantly shaped the fungal communities in soil, and increased the number of AMF ASVs in clary sage roots. In addition, successive years of clary sage cultivation also significantly shaped both fungal and AMF communities in the soil and root biotopes. Our data provide new insights on fungal and AMF communities in the rhizospheric soil and roots of clary sage grown in TE-polluted agricultural soil.</p><p><strong>Keywords</strong>: Trace Elements-polluted soils, fungal microbiota, Salvia sclarea, arbuscular mycorrhizal fungi</p>

scholarly journals Clary Sage Cultivation and Mycorrhizal Inoculation Influence the Rhizosphere Fungal Community of an Aged Trace-Element Polluted Soil

2021 ◽  
Vol 9 (6) ◽  
pp. 1333
Author(s):  
Robin Raveau ◽  
Anissa Lounès-Hadj Sahraoui ◽  
Mohamed Hijri ◽  
Joël Fontaine
Keyword(s):  
Trace Element ◽  
Community Composition ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Illumina Miseq ◽  
Amplicon Sequencing ◽  
Fungal Communities ◽  
Rhizospheric Soil ◽  
Lead Smelter ◽  
Amf Communities

Soil fungal communities play a central role in natural systems and agroecosystems. As such, they have attracted significant research interest. However, the fungal microbiota of aromatic plants, such as clary sage (Salvia sclarea L.), remain unexplored. This is especially the case in trace element (TE)-polluted conditions and within the framework of phytomanagement approaches. The presence of high concentrations of TEs in soils can negatively affect not only microbial diversity and community composition but also plant establishment and growth. Hence, the objective of this study is to investigate the soil fungal and arbuscular mycorrhizal fungi (AMF) community composition and their changes over time in TE-polluted soils in the vicinity of a former lead smelter and under the cultivation of clary sage. We used Illumina MiSeq amplicon sequencing to evaluate the effects of in situ clary sage cultivation over two successive years, combined or not with exogenous AMF inoculation, on the rhizospheric soil and root fungal communities. We obtained 1239 and 569 fungal amplicon sequence variants (ASV), respectively, in the rhizospheric soil and roots of S. sclarea under TE-polluted conditions. Remarkably, 69 AMF species were detected at our experimental site, belonging to 12 AMF genera. Furthermore, the inoculation treatment significantly shaped the fungal communities in soil and increased the number of AMF ASVs in clary sage roots. In addition, clary sage cultivation over successive years could be one of the explanatory parameters for the inter-annual variation in both fungal and AMF communities in the soil and root biotopes. Our data provide new insights on fungal and AMF communities in the rhizospheric soil and roots of an aromatic plant, clary sage, grown in TE-polluted agricultural soil.

scholarly journals Meta-analysis of Lamiaceae and Euphorbiaceae medicinal plants inoculated with arbuscular mycorrhizal fungi

10.21475/na ◽  
2019 ◽  
Vol 13 ((04) 2019) ◽  
pp. 588-598
Author(s):  
Sonivaldo Ruzzene Beltrame ◽  
Rayane Monique Sete da Cruz ◽  
Emerson Luiz Botelho Lourenço ◽  
Odair Alberton
Keyword(s):  
Medicinal Plants ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Meta Analysis ◽  
Arbuscular Mycorrhizal ◽  
Dry Mass ◽  
Control Group ◽  
P Accumulation ◽  
Ornamental Crops ◽  
The Mean

Lamiaceae and Euphorbiaceae are two families of plants grown as medicinal, aromatic, food and ornamental crops, and are of great commercial importance. The cultivation of these species depends on soil quality and availability of soil resources. Arbuscular mycorrhizal fungi (AMF) usually penetrates plants through their roots, supplying plants with water and nutrients and receiving photosynthesis products in return. These types of symbiosis benefit the development and production of crops. To analyze the effects of AMF inoculation on the production of plants of these families, a meta - analysis was performed using 183 data of Lamiaceae plants and 68 data of Euphorbiaceae plants. Meta-analysis consists of compiling data from literature to obtain the response ratio, calculated by the mean of the experimental group divided by the mean of the control group. The response variables were shoot dry mass (SDM), total dry mass (TDM), plant height and accumulation of phosphorus in the plant shoot (P-shoot). Results showed an increased mean production of AMF-inoculated plants with a 96% and 97% increase of SDM in Lamiaceae and Euphorbiaceae, respectively. Increases of 91% in TDM and 248% in P accumulated in the plant shoot were observed for Lamiaceae; values that were higher than those of Euphorbiaceae at 110% and 675%, respectively. This meta-analysis confirmed the potential of AMF to increase biomass production and P accumulation in medicinal plants of the Lamiaceae and Euphorbiaceae families.

scholarly journals Mycorrhizal fungi Glomus spp. propagation in zeolite enriched with mycorrhiza helper bacteria for controlling nematode in coffee

2021 ◽  
Vol 883 (1) ◽  
pp. 012021
Author(s):  
I N Asyiah ◽  
R Hindersah ◽  
R Harni ◽  
B N Fitriatin ◽  
W Anggraeni
Keyword(s):  
Bacillus Subtilis ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Maize Seedling ◽  
Spore Density ◽  
Glomus Spp ◽  
Spore Population ◽  
Pseudomonas Diminuta ◽  
Different Levels ◽  
Liquid Inoculant

Abstract Arbuscular mycorrhizal fungi (AMF) play a role in suppressing the nematode Pratylenchus coffeae. Mycorrhizal helper bacteria (MHB) can increase the effectiveness of AMF to control the diseases. The experimental purpose was to increase the spore population of AMF Glomus spp. in zeolite-based formulation inoculated with liquid consortia of Pseudomonas diminuta and Bacillus subtilis as MHB. The experimental design was a completely random design with six treatments consisted of 106, 107, 108, and 109 CFU/mL MHB liquid inoculants. The control treatments were water and 2% molasses. All treatments were replicated four times. A total of 300 mL/pot Liquid inoculant of MHB have been inoculated a three day before transplanting the maize seedling to the Zeolite inoculated with Glomus spp. in the pot. One month after MHB inoculation, Glomus formulation in Zeolite with different levels of MHB increased the degree of infection. Three months after MHB inoculation, spore content in Zeolite increased. The density of P. diminuta and B. subtilis in zeolite-based mycorrhizal inoculant increased at the end of the experiment. Liquid inoculant MHB contained 108 CFU/mL enhanced spora number fourth times compared to the control. This experiment suggests that P. diminuta and B. subtilis were effective to increase the spore density of AMF inoculant.

Sign in / Sign up

Export Citation Format

Share Document