Experimental Study of Photoradiation Therapy by Argon pumped Dye Laser and Hematoporphyrin Derivative on Malignant Brain Tumor Model

✓ A digital video fluorescence microscopy technique was used to evaluate the distribution of hematoporphyrin derivative (HPD) in the rat intracerebral 9L gliosarcoma brain-tumor model at 4, 24, 48, and 72 hours after intravenous administration of 10 mg/kg of the drug. Compared to surrounding normal brain, there was significant preferential uptake of HPD into the tumor. In sections surveyed, fluorescence reached a maximum value by 24 hours; however, only 33% to 44% of the tumor was fluorescent. In contrast, fluorescence within the surrounding normal brain was maximum at 4 hours, but was present in less than 1% of the brain tissue evaluated. The effect of HPD sensitization to a laser light dose (633 nm) of 30 joules/sq cm delivered through the intact skull was evaluated histologically in 10 rats. A patchy coagulation necrosis, possibly corresponding to the distribution of HPD fluorescence seen within the tumor, was observed. There was evidence that photoradiation therapy (PRT) affects defective tumor vasculature and that a direct tumor cell toxicity spared normal brain tissue. Despite these findings, limited uptake of HPD in tumor and the brain adjacent to tumor may decrease the effectiveness of PRT in the 9L gliosarcoma brain-tumor model. Because of the similarity between the capillary system of the 9L tumor and human brain tumors, PRT may have a limited therapeutic effect in patients with malignant brain tumors.

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Hematoporphyrin derivative photoradiation therapy of the rat 9L gliosarcoma brain tumor model

Lasers in Surgery and Medicine ◽

10.1002/lsm.1900040114 ◽

1984 ◽

Vol 4 (1) ◽

pp. 99-105 ◽

Cited By ~ 10

Author(s):

James E. Boggan ◽

Michael S. B. Edwards ◽

Catherine A. Bolger ◽

Michael W. Berns ◽

Robert J. Walter

Keyword(s):

Brain Tumor ◽

Tumor Model ◽

Hematoporphyrin Derivative ◽

9L Gliosarcoma ◽

Brain Tumor Model

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Ultrasmall Core-Shell Silica Nanoparticles for Precision Drug Delivery in a High-Grade Malignant Brain Tumor Model

Clinical Cancer Research ◽

10.1158/1078-0432.ccr-19-1834 ◽

2019 ◽

Vol 26 (1) ◽

pp. 147-158 ◽

Cited By ~ 10

Author(s):

Rupa Juthani ◽

Brian Madajewski ◽

Barney Yoo ◽

Li Zhang ◽

Pei-Ming Chen ◽

...

Keyword(s):

Drug Delivery ◽

Brain Tumor ◽

Silica Nanoparticles ◽

Tumor Model ◽

Malignant Brain Tumor ◽

Core Shell ◽

High Grade ◽

Brain Tumor Model

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Effect of hematoporphyrin derivative photoradiation therapy on survival in the rat 9L gliosarcoma brain-tumor model

Journal of Neurosurgery ◽

10.3171/jns.1985.63.6.0917 ◽

1985 ◽

Vol 63 (6) ◽

pp. 917-921 ◽

Cited By ~ 28

Author(s):

James E. Boggan ◽

Catherine Bolger ◽

Michael S. B. Edwards

Keyword(s):

Brain Tumor ◽

Laser Light ◽

Tumor Model ◽

Hematoporphyrin Derivative ◽

Survival Times ◽

Content Type ◽

9L Gliosarcoma ◽

Brain Tumor Cells ◽

Tumor Region ◽

Brain Tumor Model

✓ Intracerebral tumors were produced in 99 rats by stereotaxic implantation of 9L gliosarcoma brain-tumor cells. Hematoporphyrin derivative (HPD), 10 or 20 mg/kg, was administered as an intravenous bolus 24 or 48 hours before irradiation of the tumor region with light from an argon pumped-dye laser (632 nm). Laser light, at a dose of 30, 60, or 200 joules/sq cm, was delivered through a craniectomy 10 or 13 days after tumor implantation. Survival times were significantly prolonged in rats exposed to laser light at a dose of 200 joules/sq cm 24 hours after administration of HPD, 20 mg/kg.

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Quantitative Measurements of Regional Glucose Utilization and Rate of Valine Incorporation into Proteins by Double-Tracer Autoradiography in the Rat Brain Tumor Model

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/jcbfm.1989.12 ◽

1989 ◽

Vol 9 (1) ◽

pp. 87-95 ◽

Cited By ~ 22

Author(s):

Michihiro Kirikae ◽

Mirko Diksic ◽

Y. Lucas Yamamoto

Keyword(s):

Protein Synthesis ◽

Brain Tumor ◽

Rat Brain ◽

Brain Tissue ◽

Glucose Utilization ◽

Tumor Model ◽

Normal Brain ◽

Normal Brain Tissue ◽

Rat Brain Tumor ◽

Brain Tumor Model

We examined the rate of glucose utilization and the rate of valine incorporation into proteins using 2-[18F]fluoro-2-deoxyglucose and L-[1-14C]-valine in a rat brain tumor model by quantitative double-tracer autoradiography. We found that in the implanted tumor the rate of valine incorporation into proteins was about 22 times and the rate of glucose utilization was about 1.5 times that in the contralateral cortex. (In the ipsilateral cortex, the tumor had a profound effect on glucose utilization but no effect on the rate of valine incorporation into proteins.) Our findings suggest that it is more useful to measure protein synthesis than glucose utilization to assess the effectiveness of antitumor agents and their toxicity to normal brain tissue. We compared two methods to estimate the rate of valine incorporation: “kinetic” (quantitation done using an operational equation and the average brain rate coefficients) and “washed slices” (unbound labeled valine removed by washing brain slices in 10% thrichloroacetic acid). The results were the same using either method. It would seem that the kinetic method can thus be used for quantitative measurement of protein synthesis in brain tumors and normal brain tissue using [11C]-valine with positron emission tomography.

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