scholarly journals Pharmacological Effect of Verapamil on Brain Oxidative Parameters and Mania-Like Behaviour in Paradoxical Sleep Deprived Mice

2021 ◽  
Vol 14 (4) ◽  
pp. 1486-1492
Author(s):  
Anthony T. Eduviere
Keyword(s):  
Oxidative Stress ◽  
Sleep Deprivation ◽  
Paradoxical Sleep ◽  
Calcium Signalling ◽  
Channel Blockers ◽  
Reference Drug ◽  
Treatment Groups ◽  
Group 5 ◽  
Behavioural Tests ◽  
Stress Damage

Although the implication of calcium signalling in the aetiology of anxiety remains elusive, drugs modulating calcium (like calcium channel blockers) have been discovered to be some worth beneficial as treatment option for anxiety related disorders. This study was therefore undertaken to assess probable ameliorative potential of verapamil against manic-like (stereotype behaviour) and anxiety-like symptoms in mice exposed to sleep deprivation. Mice were allotted into five treatment groups (n=5): group 1 and 2 received 10 mL/kg distilled water, groups 3 and 4 verapamil (25 and 50 mg/kg) while group 5 received astaxanthin (50 mg/kg) which served as the reference drug. Treatment was for 7 days and animals were sleep-deprived on the final 72 hours. Various behavioural tests to determine degree of stereotypical behaviour and locomotor activity were carried out. Anxiety test was done via the aid of a light/dark box and plus maze while stereotype behaviour was assess utilizing an open field box. Oxidative stress parameters; malondialdehyde and glutathione were assessed. Histopathological perturbations in the caudate putamen were also recorded. Data were subjected to ANOVA at α0.05. The results obtained suggest that verapamil significantly suppressed stereotyped behaviour and reduced the incidence of manic-like behaviour which was induced by paradoxical sleep deprivation. Verapamil also significantly restored antioxidant levels and protected against loss of caudate neurons. In conclusion, verapamil ameliorates manic-like symptoms and anxiety in mice derived of sleep, while protecting brain neurons against oxidative stress damage induced by sleep deprivation.

Quercetin reduces manic-like behavior and brain oxidative stress induced by paradoxical sleep deprivation in mice

2016 ◽  
Vol 99 ◽  
pp. 79-86 ◽  
Author(s):  
Luiz K.S. Kanazawa ◽  
Débora D. Vecchia ◽  
Etiéli M. Wendler ◽  
Palloma de A.S. Hocayen ◽  
Francislaine A. dos Reis Lívero ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sleep Deprivation ◽  
Paradoxical Sleep ◽  
Paradoxical Sleep Deprivation ◽  
Brain Oxidative Stress

scholarly journals Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats

2015 ◽  
Vol 34 (2) ◽  
pp. 87 ◽  
Author(s):  
Fitranto Arjadi ◽  
Ginus Partadireja ◽  
Lientje Setyawan Maurits ◽  
Mulyono Pangestu
Keyword(s):  
Sleep Deprivation ◽  
Caspase 3 ◽  
Paradoxical Sleep ◽  
White Male ◽  
Male Rats ◽  
Paradoxical Sleep Deprivation ◽  
Treatment Groups ◽  
Footshock Stress ◽  
Post Hoc ◽  
One Way Anova

BACKGROUND<br />Sleep deprivation is a significant problem among adult men and is considered as a risk factor for several diseases. Paradoxical sleep deprivation (PSD) induces Leydig cell apoptosis through elevation of corticosterone, with testicular malondialdehyde (MDA) and Leydig cell caspase-3 expression as parameters. The aim of this study was to observe testicular MDA level and caspase-3 expression treated with paradoxical sleep deprivation (PSD), immobilization, and footshock stress and to determine the stress model with a significant effect in white male rats (Rattus norvegicus) . <br /><br />METHODS<br />This experimental randomized study of posttest only with control group design was conducted on 24 white male Wistar strain rats, randomly allocated into four treatment groups, i.e. control (K1) without any stress treatment, PSD (KII), immobilization (KIII), and footshock stress (KIV). Treatments were given for 25 days to produce chronic stress. Testicular MDA concentration was examined by the ELISA method while caspase-3 was examined by the TUNEL method.<br /><br />RESULTS<br />Mean testicular MDA concentration with one-way ANOVA test showed differences in means between the groups (p=0.000) and post hoc Tukey-HSD test showed significant results between PSD stress group versus control, immobilization and footshock stress groups. One-way ANOVA test showed a significant difference in caspase-3 expression in at least two treatment groups (p=0.008) and post-hoc Tuckey-LSD test showed significant differences between controls and all stress groups. <br /><br />CONCLUSION<br />Sleep deprivation is a type of stress inducing changes in testicular MDA concentration and caspase-3 expression in male rat testes.

scholarly journals Differential effects of paradoxical sleep deprivation on memory and oxidative stress

2014 ◽  
Vol 387 (5) ◽  
pp. 399-406 ◽  
Author(s):  
Alisson Menezes Araujo Lima ◽  
Veralice Meireles Sales de Bruin ◽  
Emiliano Ricardo Vasconcelos Rios ◽  
Pedro Felipe Carvalhedo de Bruin
Keyword(s):  
Oxidative Stress ◽  
Sleep Deprivation ◽  
Paradoxical Sleep ◽  
Paradoxical Sleep Deprivation ◽  
Differential Effects ◽  
And Oxidative Stress

Absence of oxidative stress following paradoxical sleep deprivation in rats

1997 ◽  
Vol 235 (1-2) ◽  
pp. 25-28 ◽  
Author(s):  
Vânia D'Almeida ◽  
Débora C Hipólide ◽  
Lı́gia A Azzalis ◽  
Letı́cia L Lobo ◽  
Virgı́nia B.C Junqueira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sleep Deprivation ◽  
Paradoxical Sleep ◽  
Paradoxical Sleep Deprivation

Paradoxical sleep deprivation induces oxidative stress in the submandibular glands of Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Taye J. Lasisi ◽  
Shehu-Tijani T. Shittu ◽  
Jude I. Abeje ◽  
Kehinde J. Ogunremi ◽  
Seyyid A. Shittu
Keyword(s):  
Oxidative Stress ◽  
Sleep Deprivation ◽  
Wistar Rats ◽  
Paradoxical Sleep ◽  
Salivary Secretion ◽  
Control Group ◽  
Paradoxical Sleep Deprivation ◽  
Sod Activity ◽  
Submandibular Glands ◽  
Male Wistar Rats

Abstract Objectives Paradoxical sleep deprivation has been associated with impaired salivary secretion in rats. However, the mechanism that underlies this is not known. Therefore, this study assessed salivary and serum oxidative stress levels following paradoxical sleep deprivation in rats. Methods Twenty-one male Wistar rats randomly divided into three groups of seven rats each as; Control (C); partial sleep-deprived (PSD); and total sleep-deprived (TSD) were used. Malondialdehyde (MDA) concentration, Superoxide dismutase (SOD), and catalase activities were evaluated in saliva, serum, and submandibular glands after seven days of sleep deprivation. Data were expressed as mean ± standard error of the mean and analyzed using one-way ANOVA, Tukey HSD post hoc, and Pearson’s correlation tests. Results Serum MDA levels were significantly higher in both the TSD and PSD groups compared to the control group whereas only the TSD group showed higher submandibular MDA levels compared to the PSD group and the control group. Submandibular SOD activity was significantly lower in both the TSD and PSD groups compared to the control group. Serum catalase activity was significantly lower in the TSD group only compared to the control group. Conclusions These results have demonstrated for the first time that paradoxical sleep deprivation was associated with changes in the oxidant/antioxidant defense system in the submandibular salivary glands of male Wistar rats which may contribute to impairment in salivary secretion.

Physical exercise protects dynamic balance and motor coordination of rats treated with vincristine

2020 ◽  
Vol 19 (5) ◽  
pp. 336
Author(s):  
Luiza Minato Sagrillo ◽  
Viviane Nogueira De Zorzi ◽  
Luiz Fernando Freire Royes ◽  
Michele Rechia Fighera ◽  
Beatriz Da Silva Rosa Bonadiman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Locomotor Activity ◽  
Physical Exercise ◽  
Motor Coordination ◽  
Dynamic Balance ◽  
Exercise Group ◽  
Adult Rats ◽  
The Central Nervous System ◽  
Stress Damage ◽  
Training Protocol

Physical exercise has been shown to be an important modulator of the antioxidant system and neuroprotective in several diseases and treatments that affect the central nervous system. In this sense, the present study aimed to evaluate the effect of physical exercise in dynamic balance, motor coordination, exploratory locomotor activity and in the oxidative and immunological balance of rats treated with vincristine (VCR). For that, 40 adult rats were divided into two groups: exercise group (6 weeks of swimming, 1h/day, 5 days/week, with overload of 5% of body weight) and sedentary group. After training, rats were treated with 0.5 mg/kg of vincristine sulfate for two weeks or with the same dose of 0.9% NaCl. The behavioral tests were conducted 1 and 7 days after each dose of VCR. On day 15 we carried out the biochemical analyzes of the cerebellum. The physical exercise was able to protect against the loss of dynamic balance and motor coordination and, had effect per se in the exploratory locomotor activity, and neutralize oxidative stress, damage DNA and immune damage caused by VCR up to 15 days after the end of the training protocol. In conclusion, we observed that previous physical training protects of the damage motor induced by vincristine.Key-words: exercise, oxidative stress, neuroprotection, cerebellum.

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