scholarly journals Genetic diversity of two stingless bees, Trigona nigerrima (Cresson 1878) and Trigona corvina (Cockerell 1913), in coffee dominated landscapes in southern Mexico

2015 ◽  
Vol 31 (1) ◽  
Author(s):  
Erik De Jesús Solórzano-Gordillo ◽  
Nina Vanessa Cabrera-Marín ◽  
José Mérida ◽  
Remy Benoit Marie Vandame ◽  
Daniel Sánchez
Keyword(s):  
Genetic Diversity ◽  
Population Genetics ◽  
Honey Bees ◽  
Stingless Bees ◽  
Stingless Bee ◽  
Bumble Bees ◽  
Southern Mexico ◽  
Expected Heterozygosity ◽  
Allele Richness ◽  
Genetic Status

Stingless bees (Apidae, Meliponini) are key pollinators in natural and agricultural Pantropical environments. Current human activities, however, threaten their populations, making it urgent to assesstheir abundance and genetic status. By a population genetics approach, we calculated the genetic diversity and estimated the number of wild colonies of two stingless bee species, Trigona nigerrima (Cresson) and T. corvina (Cockerell) in southern Mexico. Allele richness ranged from 10 to 19 (mean ± SD = 14 ± 3.5) for both species. Expected heterozygosity ranged 0.88-0.93 for T. corvina and 0.82-0.92 for T. nigerrima. The estimated number of colonies estimated was 25 on average for T. corvina and 21 for T. nigerrima. According to our results populations of both species seem not to be in danger. We also show that genetic tools can be extremely useful for colony density estimation for stingless bees as it is for bumble bees and honey bees.

scholarly journals Population Growth and Insecticide Residues of Honey Bees in Tropical Agricultural Landscapes

Diversity ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 1 ◽  
Author(s):  
Damayanti Buchori ◽  
Akhmad Rizali ◽  
Windra Priawandiputra ◽  
Dewi Sartiami ◽  
Midzon Johannis
Keyword(s):  
Land Use ◽  
Population Growth ◽  
Honey Bees ◽  
Agricultural Intensification ◽  
Stingless Bees ◽  
Field Experiments ◽  
Habitat Type ◽  
Agricultural Landscapes ◽  
Stingless Bee ◽  
Home Garden

Global decline of pollinators, especially bees, has been documented in many countries. Several causes such as land-use change and agricultural intensification are reported to be the main drivers of the decline. The objective of this study was to investigate the effect of land use on honey bee and stingless bee populations. Research was conducted in Bogor and Malang to compare between two different geographical areas. Managed bees such as honey bees (Apis cerana and A. mellifera) and stingless bees (Tetragonula laeviceps) were investigated to examine the effect of agricultural intensification. Field experiments were conducted by placing beehives in selected habitats (i.e., beekeeper gardens, forests areas, and agriculture areas). Population growth and neonicotinoid residue analysis of bees in different hive locations were measured to study the effect of habitat type. Population growth of bees represents the forager abundance and colony weight. Based on the analysis, we found that habitat type affected forager abundance and colony weight of honey bees (p < 0.05), although the patterns were different between species, region, as well as season. Forests could support the stingless bee colony better than agriculture and home garden habitats. Insecticide (neonicotinoid) was barely recorded in both honey bees and stingless bees.

scholarly journals Occurrence and Phylogenetic Analysis of DWV in Stingless Bee (Apidae sp.) in China: A Case Report

2021 ◽  
Vol 1 ◽  
Author(s):  
Lina Zhang ◽  
Yanchun Deng ◽  
Hongxia Zhao ◽  
Ming Zhang ◽  
Chunsheng Hou
Keyword(s):  
Phylogenetic Analysis ◽  
Honey Bee ◽  
Honey Bees ◽  
Stingless Bees ◽  
Vital Role ◽  
Stingless Bee ◽  
Pollination Services ◽  
Important Indicator ◽  
Bee Colonies ◽  
Bee Viruses

Honey bees play a vital role in providing pollination services for agricultural crops and wild flowering plants. However, the spillover risk of their pathogens to other pollinators or wild insects is becoming a cause for concern. There is some evidence that stingless bees can carry honey bee viruses, but little is known about the presence of honey bee viruses in stingless bees in China. Here, we investigate the occurrence of major honey bee pathogens including bacteria, fungi, and viruses in stingless bees (Apidae: sp.). Our results show that the stingless bees (Apidae: sp.) were mainly infected with DWV-A, but no DWV-B and DWV-C. Phylogenetic analysis on fragments of lp, RdRp, and VP3 of DWV-A indicated that genetic variation in VP3 might an important indicator for host-specific viruses, but it requires further study. Our results indicated that DWV-A is not only the major strain of virus currently circulating in managed bee colonies in China and globally, but in stingless bee species as a whole.

scholarly journals The content of propolis’s flavonoid from two species of stingless bee in Lombok

2021 ◽  
Vol 914 (1) ◽  
pp. 012064
Author(s):  
N Wahyuni ◽  
S D Riendriasari
Keyword(s):  
Honey Bees ◽  
Stingless Bees ◽  
Stingless Bee ◽  
Aqueous Extraction ◽  
Flavonoid Content ◽  
Great Demand ◽  
The People ◽  
Young Leaves

Abstract Today’s stingless bees are in great demand by beekeepers in Lombok. Besides producing honey, it also produces propolis which has more value. Propolis or bee glue is a substance produced by honey bees collected from the top young leaves mixed with the saliva, patch, and hive sterilization. This substance could be used for antibacterial, antivirus, antifungal, and anti-protozoa. In Lombok, apiculture for stingless bees has spread in almost every district. Northern Lombok, West Lombok, and East Lombok were the first three locations that developed stingless bees. This study aimed to identify the species of stingless bees and their propolis productivity and characteristics produced by each species. There were two species of stingless bees cultivated by the people of Lombok, Trigona clypearis and Trigona sapiens. The development of science, genus Trigona changed nomenclature into Tetragonula. The yield of propolis through extraction using water or Aqueous Extraction Propolis (AEP) produced by T. clypearis was 34-55% and T. sapiens was in the range of 3-24%. The flavonoid content equivalent to quercetin in propolis produced by T. sapiens as vary as T. clypearis. The results of this study could be used as a baseline for other propolis extraction activities to produce propolis more marketable.

scholarly journals Nosema ceranae causes cellular immunosuppression and interacts with thiamethoxam to increase mortality in the stingless bee Melipona colimana

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
José O. Macías-Macías ◽  
José C. Tapia-Rivera ◽  
Alvaro De la Mora ◽  
José M. Tapia-González ◽  
Francisca Contreras-Escareño ◽  
...  
Keyword(s):  
Honey Bees ◽  
Stingless Bees ◽  
Inhibitory Effect ◽  
Stingless Bee ◽  
Nosema Ceranae ◽  
Limited Information ◽  
Microsporidian Parasite ◽  
Field Exposure ◽  
Exposure Levels ◽  
And Control

Abstract The microsporidian parasite Nosema ceranae and neonicotinoid insecticides affect the health of honey bees (Apis mellifera). However, there is limited information about the effect of these stressors on other pollinators such as stingless bees (Hymenoptera: Meliponini). We examined the separate and combined effects of N. ceranae and the neonicotinoid thiamethoxam at field-exposure levels on the survivorship and cellular immunity (hemocyte concentration) of the stingless bee Melipona colimana. Newly-emerged bees were subjected to four treatments provided in sucrose syrup: N. ceranae spores, thiamethoxam, thiamethoxam and N. ceranae, and control (bees receiving only syrup). N. ceranae developed infections of > 467,000 spores/bee in the group treated with spores only. However, in the bees subjected to both stressors, infections were < 143,000 spores/bee, likely due to an inhibitory effect of thiamethoxam on the microsporidium. N. ceranae infections did not affect bee survivorship, but thiamethoxam plus N. ceranae significantly increased mortality. Hemocyte counts were significantly lower in N. ceranae infected-bees than in the other treatments. These results suggest that N. ceranae may infect, proliferate and cause cellular immunosuppression in stingless bees, that exposure to sublethal thiamethoxam concentrations is toxic to M. colimana when infected with N. ceranae, and that thiamethoxam restrains N. ceranae proliferation. These findings have implications on pollinators’ conservation.

scholarly journals Identification of Plant Origin of Propolis from Thailand Stingless Bees by Comparative Analysis

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Eriko Ishizu ◽  
Sari Honda ◽  
Boonyadist Vongsak ◽  
Shigenori Kumazawa
Keyword(s):  
Comparative Analysis ◽  
Honey Bees ◽  
High Performance ◽  
Stingless Bees ◽  
Reversed Phase ◽  
Stingless Bee ◽  
Plant Origin ◽  
Garcinia Mangostana ◽  
Ethanol Extracts ◽  
Fruit Surface

Propolis is a resinous mixture of substances collected by honey bees from certain plants. It has gained popularity as a food and alternative medicine. We recently obtained Thailand stingless bee propolis. However, to the best of our knowledge, there are only a few studies on native Thailand stingless bee propolis. The purpose of this study was to analyze the composition of Thailand stingless bee propolis and to identify its plant origin. Comparative analysis of the 70% ethanol extracts of Thailand stingless bees propolis and the yellow resin from the fruit surface of Garcinia mangostana was performed using reversed-phase high-performance liquid chromatography coupled with high resolution-electrospray mass spectrometry. The extracts showed the similar chromatographic patterns. In conclusion, we suggest that the plant origin of Thailand stingless bee propolis is the yellow resin from fruit surface of G. mangostana.

scholarly journals Microsatellite analysis reveals low genetic diversity in managed populations of the critically endangered gharial (Gavialis gangeticus) in India

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Surya Prasad Sharma ◽  
Mirza Ghazanfarullah Ghazi ◽  
Suyash Katdare ◽  
Niladri Dasgupta ◽  
Samrat Mondol ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Indian Subcontinent ◽  
Population Decline ◽  
Mitochondrial Control Region ◽  
Critically Endangered ◽  
Term Survival ◽  
Low Level ◽  
Genetic Status ◽  
In The Wild ◽  
Gavialis Gangeticus

AbstractThe gharial (Gavialis gangeticus) is a critically endangered crocodylian, endemic to the Indian subcontinent. The species has experienced severe population decline during the twentieth century owing to habitat loss, poaching, and mortalities in passive fishing. Its extant populations have largely recovered through translocation programmes initiated in 1975. Understanding the genetic status of these populations is crucial for evaluating the effectiveness of the ongoing conservation efforts. This study assessed the genetic diversity, population structure, and evidence of genetic bottlenecks of the two managed populations inhabiting the Chambal and Girwa Rivers, which hold nearly 80% of the global gharial populations. We used seven polymorphic nuclear microsatellite loci and a 520 bp partial fragment of the mitochondrial control region (CR). The overall mean allelic richness (Ar) was 2.80 ± 0.40, and the observed (Ho) and expected (He) heterozygosities were 0.40 ± 0.05 and 0.39 ± 0.05, respectively. We observed low levels of genetic differentiation between populations (FST = 0.039, P < 0.05; G’ST = 0.058, P < 0.05 Jost’s D = 0.016, P < 0.05). The bottleneck analysis using the M ratio (Chambal = 0.31 ± 0.06; Girwa = 0.41 ± 0.12) suggested the presence of a genetic bottleneck in both populations. The mitochondrial CR also showed a low level of variation, with two haplotypes observed in the Girwa population. This study highlights the low level of genetic diversity in the two largest managed gharial populations in the wild. Hence, it is recommended to assess the genetic status of extant wild and captive gharial populations for planning future translocation programmes to ensure long-term survival in the wild.

scholarly journals Olfactory coding in the antennal lobe of the bumble bee Bombus terrestris

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marcel Mertes ◽  
Julie Carcaud ◽  
Jean-Christophe Sandoz
Keyword(s):  
Honey Bees ◽  
Antennal Lobe ◽  
Bombus Terrestris ◽  
Bumble Bees ◽  
Foraging Strategies ◽  
Bumble Bee ◽  
Carbon Chain Length ◽  
Olfactory Coding ◽  
Major Transitions

AbstractSociality is classified as one of the major transitions in evolution, with the largest number of eusocial species found in the insect order Hymenoptera, including the Apini (honey bees) and the Bombini (bumble bees). Bumble bees and honey bees not only differ in their social organization and foraging strategies, but comparative analyses of their genomes demonstrated that bumble bees have a slightly less diverse family of olfactory receptors than honey bees, suggesting that their olfactory abilities have adapted to different social and/or ecological conditions. However, unfortunately, no precise comparison of olfactory coding has been performed so far between honey bees and bumble bees, and little is known about the rules underlying olfactory coding in the bumble bee brain. In this study, we used in vivo calcium imaging to study olfactory coding of a panel of floral odorants in the antennal lobe of the bumble bee Bombus terrestris. Our results show that odorants induce reproducible neuronal activity in the bumble bee antennal lobe. Each odorant evokes a different glomerular activity pattern revealing this molecule’s chemical structure, i.e. its carbon chain length and functional group. In addition, pairwise similarity among odor representations are conserved in bumble bees and honey bees. This study thus suggests that bumble bees, like honey bees, are equipped to respond to odorants according to their chemical features.

scholarly journals Genetic diversity and population structure of date palms (Phoenix dactylifera L.) in Ethiopia using microsatellite markers

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Workia Ahmed ◽  
Tileye Feyissa ◽  
Kassahun Tesfaye ◽  
Sumaira Farrakh
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Information Content ◽  
Date Palm ◽  
Polymorphic Information Content ◽  
Phoenix Dactylifera ◽  
Mean Value ◽  
Date Palms ◽  
Expected Heterozygosity

Abstract Background Date palm tree (Phoenix dactylifera L.) is a perennial monocotyledonous plant belonging to the Arecaceae family, a special plant with extraordinary nature that gives eminent contributions in agricultural sustainability and huge socio-economic value in many countries of the world including Ethiopia. Evaluation of genetic diversity across date palms at DNA level is very important for breeding and conservation. The result of this study could help to design for genetic improvement and develop germplasm introduction programmes of date palms mainly in Ethiopia. Results In this study, 124 date palm genotypes were collected, and 10 polymorphic microsatellite markers were used. Among 10 microsatellites, MPdCIR085 and MPdCIR093 loci showed the highest value of observed and expected heterozygosity, maximum number of alleles, and highest polymorphic information content values. A total of 112 number of alleles were found, and the mean number of major allele frequency was 0.26, with numbers ranging from 0.155 (MPdCIR085) to 0.374 (MPdCIR016); effective number of alleles with a mean value of 6.61, private alleles ranged from 0.0 to 0.65; observed heterozygosity ranged from 0.355 to 0.726; expected heterozygosity varied from 0.669 to 0.906, polymorphic information content with a mean value of 0.809; fixation index individuals relative to subpopulations ranged from 0.028 for locus MPdCIR032 to 0.548 for locus MPdCIR025, while subpopulations relative to total population value ranged from − 0.007 (MPdCIR070) to 0.891 (MPdCIR015). All nine accesstions, neighbour-joining clustering analysis, based on dissimilarity coefficient values were grouped into five major categories; in population STRUCTURE analysis at highest K value, three groups were formed, whereas DAPC separated date palm genotypes into eight clusters using the first two linear discriminants. Principal coordinate analysis was explained, with a 17.33% total of variation in all populations. Generally, the result of this study revealed the presence of allele variations and high heterozygosity (> 0.7) in date palm genotypes. Conclusions Microsatellites (SSR) are one of the most preferable molecular markers for the study of genetic diversity and population structure of plants. In this study, we found the presence of genetic variations of date palm genotypes in Ethiopia; therefore, these genetic variations of date palms is important for crop improvement and conservation programmes; also, it will be used as sources of information to national and international genbanks.

scholarly journals Virus Disinfection and Population Genetics: Toward the Control of Waterborne Virus Diseases by Water Engineering

2021 ◽  
Author(s):  
Syun-suke Kadoya ◽  
Hiroyuki Katayama ◽  
Daisuke Sano
Keyword(s):  
Genetic Diversity ◽  
Population Genetics ◽  
Field Experiments ◽  
Virus Evolution ◽  
Diverse Population ◽  
Virus Population ◽  
Significant Information ◽  
Sensitivity Change ◽  
Waterborne Virus ◽  
Waterborne Viruses

Abstract Purpose of Review Major waterborne viruses comprise numerous variants rather than only a master sequence and form a genetically diverse population. High genetic diversity is advantageous for adaptation to environmental changes because the highly diverse population likely includes variants resistant to an adverse effect. Disinfection is a broadly employed tool to inactivate pathogens, but due to virus evolvability, waterborne viruses may not be inactivated sufficiently in currently applied disinfection conditions. Here, by focusing on virus population genetics, we explore possibility and factor of emergence of disinfection sensitivity change. Recent Findings To test whether virus population obtains disinfection resistance, the evolutionary experiment developed in the field of population genetics has been applied, indicating the change in disinfection sensitivity. It has been also confirmed that the sensitivity of environmental strains is lower than that of laboratory strains. In some of these studies, genetic diversity within a population less sensitive to disinfection is higher. Researches in virus population genetics have shown the contribution of intra-population genetic diversity to virus population phenotype, so disinfection sensitivity change may attribute to the genetic diversity. Summary The research elucidating a relationship between virus evolution and disinfection has only recently begun, but significant information about the relationship has been accumulated. To develop an effective disinfection strategy for the control of waterborne virus spread, we need to clarify whether disinfection practice truly affects virus outbreaks by refining both laboratory and field experiments related to virus evolution in the disinfection-exerted environment.

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