scholarly journals Циклостойкие миниатюрные термоэлектрические модули

2019 ◽  
Vol 53 (5) ◽  
pp. 604
Author(s):  
М.П. Волков ◽  
И.А. Драбкин ◽  
Л.Б. Ершова ◽  
А.А. Назаренко
Keyword(s):  
Polymerase Chain Reaction ◽  
Test Data ◽  
Medical Equipment ◽  
Dna Analysis ◽  
Heat Fluxes ◽  
Chain Reaction ◽  
Periodic Heating ◽  
Thermoelectric Modules ◽  
Heating And Cooling ◽  
Polymerase Chain

AbstractIn the paper the test data on new cycle-resistant thermoelectric modules are presented and discussed. These modules can be applied in medical equipment for polymerase chain reaction (PCR) to carry out DNA analysis with the help of rapid periodic heating and cooling of biological probes. However, high density of heat fluxes and, as a result, significant mechanical stresses in miniature thermoelectric modules involve special requirements to their reliability. The company RMT Ltd. has developed a technology for the production of highly reliable miniature thermoelectric modules that allowed them to withstand more than 500 thousand heating-cooling cycles (from 20 to 100°C) with a rate of 20°C/s and more.

PCR BY THERMAL CONVECTION

2004 ◽  
Vol 18 (16) ◽  
pp. 775-784 ◽  
Author(s):  
DIETER BRAUN
Keyword(s):  
Polymerase Chain Reaction ◽  
Thermal Convection ◽  
Dna Amplification ◽  
Reaction Vessel ◽  
Cold Region ◽  
Chain Reaction ◽  
Heating And Cooling ◽  
Highly Sensitive ◽  
Specific Amplification ◽  
Polymerase Chain

The Polymerase Chain Reaction (PCR) allows for highly sensitive and specific amplification of DNA. It is the backbone of many genetic experiments and tests. Recently, three labs independently uncovered a novel and simple way to perform a PCR reaction. Instead of repetitive heating and cooling, a temperature gradient across the reaction vessel drives thermal convection. By convection, the reaction liquid circulates between hot and cold regions of the chamber. The convection triggers DNA amplification as the DNA melts into two single strands in the hot region and replicates into twice the amount in the cold region. The amplification progresses exponentially as the convection moves on. We review the characteristics of the different approaches and show the benefits and prospects of the method.

scholarly journals 484. DNA analysis with polymerase chain reaction (PCR) of bacterial infections

1992 ◽  
Vol 48 (8) ◽  
pp. 1567
Author(s):  
Takashi Arai ◽  
Hideaki Yamamoto ◽  
Satoshi Kobayashi ◽  
Akira Yamaguchi ◽  
Katsumi Ikei
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Infections ◽  
Dna Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Kemal Kazan ◽  
John M. Manners ◽  
Don F. Cameron
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Interspecific Cross ◽  
Parental Origin ◽  
Chain Reaction ◽  
Stylosanthes Hamata ◽  
Polymerase Chain

The inheritance of random amplified polymorphic DNA (RAPD) markers generated via the polymerase chain reaction amplification of genomic DNA sequences in an F2 family of an interspecific cross between Stylosanthes hamata and S. scabra was investigated. An initial comparison between the parental species, S. hamata cv. Verano and S. scabra cv. Fitzroy, demonstrated that 34% of detected RAPD bands were polymorphic. Of 90 primers tested, 35 showed relatively simple and reliably scorable polymorphisms and were used for segregation analysis. Sixty F2 individuals were scored for the segregation of 73 RAPD markers and 55 of these markers fit a 3:1 ratio. Segregation of eight other RAPD markers deviated significantly from a 3:1 ratio. There was no bias in the inheritance of RAPD markers regarding parental origin of the segregating RAPD markers. Linkage analysis revealed 10 linkage groups containing a total of 44 RAPD loci. Another 10 RAPD markers (7 of maternal origin) that were polymorphic between the parents did not segregate in the F2 population. One of the maternally inherited RAPD bands hybridized to chloroplast DNA. Analysis of RAPD loci by DNA hybridization indicated that mainly repeated sequences were amplified. These data indicate that RAPDs are useful genetic markers in Stylosanthes spp. and they may be suitable for genetic mapping.Key words: genetic mapping, molecular markers, polymerase chain reaction, Stylosanthes hamata, Stylosanthes scabra.

Random amplified polymorphic DNA analysis in Hordeum species

Genome ◽  
1993 ◽  
Vol 36 (6) ◽  
pp. 1029-1031 ◽  
Author(s):  
Juan Manuel González ◽  
Esther Ferrer
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Fragment Pattern ◽  
Polymerase Chain ◽  
Hordeum Species

Random amplified polymorphic DNA analysis was performed by applying a set of 13 arbitrary 10-mer primers to 19 Hordeum species and subspecies. High levels of variation in fragment pattern were observed both within and among species with most of the primers used. Genetic similarities between accessions and species were calculated from the fragment patterns. The resulting phenograms confirmed previous relationships among the Hordeum species.Key words: random amplified polymorphic DNA, polymerase chain reaction, polymorphism, Hordeum.

scholarly journals Synergy between DNA polymerases increases polymerase chain reaction inhibitor tolerance in forensic DNA analysis

2010 ◽  
Vol 405 (2) ◽  
pp. 192-200 ◽  
Author(s):  
Johannes Hedman ◽  
Anders Nordgaard ◽  
Charlotte Dufva ◽  
Birgitta Rasmusson ◽  
Ricky Ansell ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Analysis ◽  
Dna Polymerases ◽  
Forensic Dna ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Inhibitor ◽  
Forensic Dna Analysis ◽  
Inhibitor Tolerance ◽  
Polymerase Chain

Application of polymerase chain reaction for detection of goats' milk adulteration by milk of cow

2001 ◽  
Vol 68 (2) ◽  
pp. 333-336 ◽  
Author(s):  
JACEK BANIA ◽  
MACIEJ UGORSKI ◽  
ANTONI POLANOWSKI ◽  
ERYK ADAMCZYK
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Analysis ◽  
Marker Gene ◽  
Meat Products ◽  
Universal Primers ◽  
Animal Origin ◽  
Chain Reaction ◽  
Specific Primers ◽  
Single Stranded Conformational Polymorphism ◽  
Polymerase Chain

Numerous methods based on DNA analysis have been employed in the food industry to monitor adulterations of food products of animal origin. Among them the most frequently used are: polymerase chain reaction (PCR) amplification of a marker gene fragment(s) with universal primers, or amplification of DNA with species-specific primers. PCR-products of different origin can be discriminated by size, restriction fragment length polymorphism (RFLP) or single stranded conformational polymorphism (SSCP) analysis. These methods have been used for identification, and differentiation between, the animal origins of raw or heat-treated meat and meat products (Chikuni et al. 1994; Meyer et al. 1994, 1995; Zehner et al. 1998; Behrens et al. 1999; Guoli et al. 1999; Hopwood et al. 1999; Matsunaga et al. 1999; Wolf et al. 1999). These approaches are also applicable to the analysis of dairy products. However, adulterations of goats' milk and its products are traditionally tested by immunological and/or electrophoretic methods (Amigo et al. 1992; Levieux & Venien, 1994; Mimmo & Pagani, 1998). So far, only a few DNA-based techniques designed to detect the presence of bovine DNA in goats' milk have been described (Plath et al. 1997; Branciari et al. 2000). This paper presents a one-step PCR procedure for detection of adulteration of goats' milk with cows' milk. The method, employing bovine-specific primers for amplification of a 274 bp fragment of cytochrome b DNA, seems to be simple, fast, specific and sensitive.

scholarly journals A Micro Polymerase Chain Reaction Module for Integrated and Portable DNA Analysis Systems

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Elisa Morganti ◽  
Cristian Collini ◽  
Cristina Potrich ◽  
Cristina Ress ◽  
Andrea Adami ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Analysis ◽  
Thermal Characterization ◽  
Chain Reaction ◽  
Element Analysis ◽  
Thermal Requirements ◽  
Chip Carrier ◽  
Polymerase Chain ◽  
Analysis System

This work deals with the design, fabrication, and thermal characterization of a disposable miniaturized Polymerase Chain Reaction (PCR) module that will be integrated in a portable and fast DNA analysis system. It is composed of two independent parts: a silicon substrate with embedded heater and thermometers and a PDMS (PolyDiMethylSiloxane) chamber reactor as disposable element; the contact between the two parts is assured by a mechanical clamping obtained using a Plastic Leaded Chip Carrier (PLCC). This PLCC is also useful, avoid the PCR mix evaporation during the thermal cycles. Finite Element Analysis was used to evaluate the thermal requirements of the device. The thermal behaviour of the device was characterized revealing that the temperature can be controlled with a precision of ±0.5°C. Different concentrations of carbon nanopowder were mixed to the PDMS curing agent in order to increase the PDMS thermal conductivity and so the temperature control accuracy.

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