scholarly journals Использование спектральной эллипсометрии и спектроскопии комбинационного рассеяния света в скрининговой диагностике колоректального рака

2019 ◽  
Vol 127 (7) ◽  
pp. 170
Author(s):  
В.Н. Кручинин ◽  
М.В. Кручинина ◽  
Я.И. Прудникова ◽  
Е.В. Спесивцев ◽  
С.В. Рыхлицкий ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Polarized Light ◽  
High Sensitivity ◽  
Optical Methods ◽  
Healthy Individuals ◽  
Semiconductor Physics ◽  
Diagnostic Approaches ◽  
Academy Of Sciences ◽  
Non Destructive ◽  
Serum Antigens

Diagnostic monitoring of groups of healthy patients and patients with colorectal cancer (CRC) at various stages of the development of the disease was carried out using Raman spectroscopy (CRS) and measuring the state of polarized light in the vicinity of surface plasmon resonance (SPR) observation conditions. For the diagnosis, the reaction of serum antigens with antibodies to serum M2-pyruvate kinase (M2-PK) was used near the observation conditions of SPR, for which we used the Ellips-SPEC spectral ellipsometric complex created in the Institute of Semiconductor Physics of the Siberian Branch of the Russian Academy of Sciences (ISP SB RAS). The complex has high sensitivity, accuracy and non-destructive impact on the sample under study. The intensities of the peaks at 1005, 1157 and 1520 cm-1 in the CRS spectra of CRC patients as compared with healthy individuals as well as the intensity of the equilibrium plasmon signal were significantly lower, correlating with the stage of the disease and the presence of metastases. It allows us to consider these optical methods as a promising diagnostic approaches in the diagnosis of CRC, including in the early stages of the disease.

scholarly journals Высокочувствительная экспрессная нелинейно-оптическая диагностика кристаллического состояния гетероструктур типа сфалерита

2021 ◽  
Vol 91 (11) ◽  
pp. 1799
Author(s):  
М.Ф. Ступак ◽  
Н.Н. Михайлов ◽  
С.А. Дворецкий ◽  
С.Н. Макаров ◽  
А.Г. Елесин ◽  
...  
Keyword(s):  
Crystalline Structure ◽  
High Sensitivity ◽  
Scientific Instrument ◽  
State University ◽  
Semiconductor Physics ◽  
Siberian Branch ◽  
Russian Academy Of Sciences ◽  
Micro Particles ◽  
Order Of Magnitude ◽  
Academy Of Sciences

Stupak M.F.1,3, Mikhailov N.N.2,3, Dvoretsky S.A.2,4, Makarov S.N.1, Yelesin A.G.1, Verhoglyad A.G.1 1 Technological Design Institute of Scientific Instrument Engineering of the Siberian Branch of the Russian Academy of Sciences (TDI SIE SB RAS), Novosibirsk, 630058, Russian, 41 2 The Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Sciences (ISP SB RAS), Novosibirsk, 630090, etc. Akad. Lavrentieva, 13 3 Novosibirsk State University (NSU), Novosibirsk, 630090, Pirogov, 2 4 Tomsk State University (TSU), 634050, Tomsk, Etc. Lenin, 36 Tel: 7 913 948-9824, e. Mail: [email protected] Annotation. The characteristics of a highly sensitive express stand of non-linear-optical diagnosis of crystalline structures such as sphalerite by generation of the second harmonica are presented. The analysis of quantitative and qualitative characterization capabilities was carried out using the stand of features of crystalline parameters of layers of heteroepitaxial structures cdhHg1-xTe on substralis from GaAs with orientation (013). The results were obtained by deviations of orientation in layers from the orientation of the substrate, which arose during the epitaxy, to determine the existence of stresses. The high sensitivity of the stand revealed the presence/absence of micro-particles with a disordered crystalline structure. Experimental results of reversible modification of the "in situ" crystalline state of CdxHg1-xTe structures with short-term local radiation exposure of high power laser radiation are given. New experimental data have been presented showing that the components of the nonlineaic susceptibility tensor cxyz(w) of the crystalline structure of CdxHg1-xTe depend on composition and are an order of magnitude larger than similar components of tensor in CdTe and GaAs.

scholarly journals Высокочувствительная экспрессная нелинейно-оптическая диагностика кристаллического состояния гетероструктур типа сфалерита

2021 ◽  
Vol 91 (8) ◽  
pp. 1299
Author(s):  
М.Ф. Ступак ◽  
Н.Н. Михайлов ◽  
С.А. Дворецкий ◽  
С.Н. Макаров ◽  
А.Г. Елесин ◽  
...  
Keyword(s):  
Crystalline Structure ◽  
High Sensitivity ◽  
Scientific Instrument ◽  
State University ◽  
Semiconductor Physics ◽  
Siberian Branch ◽  
Russian Academy Of Sciences ◽  
Micro Particles ◽  
Order Of Magnitude ◽  
Academy Of Sciences

Stupak M.F.1,3, Mikhailov N.N.2,3, Dvoretsky S.A.2,4, Makarov S.N.1, Yelesin A.G.1, Verhoglyad A.G.1 1 Technological Design Institute of Scientific Instrument Engineering of the Siberian Branch of the Russian Academy of Sciences (TDI SIE SB RAS), Novosibirsk, 630058, Russian, 41 2 The Institute of Semiconductor Physics, Siberian Branch of the Russian Academy of Sciences (ISP SB RAS), Novosibirsk, 630090, etc. Akad. Lavrentieva, 13 3 Novosibirsk State University (NSU), Novosibirsk, 630090, Pirogov, 2 4 Tomsk State University (TSU), 634050, Tomsk, Etc. Lenin, 36 Tel: 7 913 948-9824, e. Mail: [email protected] Annotation. The characteristics of a highly sensitive express stand of non-linear-optical diagnosis of crystalline structures such as sphalerite by generation of the second harmonica are presented. The analysis of quantitative and qualitative characterization capabilities was carried out using the stand of features of crystalline parameters of layers of heteroepitaxial structures cdhHg1-xTe on substralis from GaAs with orientation (013). The results were obtained by deviations of orientation in layers from the orientation of the substrate, which arose during the epitaxy, to determine the existence of stresses. The high sensitivity of the stand revealed the presence/absence of micro-particles with a disordered crystalline structure. Experimental results of reversible modification of the "in situ" crystalline state of CdxHg1-xTe structures with short-term local radiation exposure of high power laser radiation are given. New experimental data have been presented showing that the components of the nonlineaic susceptibility tensor cxyz(w) of the crystalline structure of CdxHg1-xTe depend on composition and are an order of magnitude larger than similar components of tensor in CdTe and GaAs.

What Optical Metrology Can Do for Experimental Mechanics ?

2011 ◽  
Vol 70 ◽  
pp. 1-20
Author(s):  
Wolfgang Osten
Keyword(s):  
High Sensitivity ◽  
Fast Response ◽  
Experimental Mechanics ◽  
Optical Methods ◽  
Optical Metrology ◽  
Identification Problems ◽  
Destructive Testing ◽  
Experimental Stress Analysis ◽  
Versatile Tool ◽  
Non Destructive

Optical metrology has shown to be a versatile tool for the solution of many measurement and inspection problems. The main advantages of optical methods are the non-contact nature, the non-destructive and areal working principle, the fast response, high sensitivity, resolution and accuracy. Consequently, optical principles are increasingly being considered in many areas where reliable data about the shape, the surface properties, the state of stress and the strength of the object under test have to be acquired. However, these advantages have to be paid with some serious disadvantages that are mainly connected with the poor features of identification problems. In this article several examples are presented where optical metrology is helpful for experimental mechanics. The presentation is mainly focused on two topics: the acquisition of quantitative data for experimental stress analysis and the solution of inspection problems by holographic non-destructive testing. Some current aspects such as modern approaches for the solution of identification problems, the installation of remote laboratories and the calibration of measurement set-ups by specially engineered calibration samples are discussed finally.

Detector-C: A Blood-based IVD with High Sensitivity and Specificity for Early Detection and Screening of Colorectal Cancer

2010 ◽  
Vol 48 (08) ◽  
Author(s):  
A Rosenthal ◽  
H Köppen ◽  
R Musikowski ◽  
R Schwanitz ◽  
J Behrendt ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Early Detection ◽  
Sensitivity And Specificity ◽  
High Sensitivity

scholarly journals Rock Emissivity Measurement for Infrared Thermography Engineering Geological Applications

2021 ◽  
Vol 11 (9) ◽  
pp. 3773
Author(s):  
Simone Mineo ◽  
Giovanna Pappalardo
Keyword(s):  
Surface Temperature ◽  
Infrared Thermography ◽  
High Sensitivity ◽  
Point Of View ◽  
Rock Surface ◽  
Rock Types ◽  
Ornamental Stones ◽  
Emissivity Measurement ◽  
Laboratory Tool ◽  
Non Destructive

Infrared thermography is a growing technology in the engineering geological field both for the remote survey of rock masses and as a laboratory tool for the non-destructive characterization of intact rock. In this latter case, its utility can be found either from a qualitative point of view, highlighting thermal contrasts on the rock surface, or from a quantitative point of view, involving the study of the surface temperature variations. Since the surface temperature of an object is proportional to its emissivity, the knowledge of this last value is crucial for the correct calibration of the instrument and for the achievement of reliable thermal outcomes. Although rock emissivity can be measured according to specific procedures, there is not always the time or possibility to carry out such measurements. Therefore, referring to reliable literature values is useful. In this frame, this paper aims at providing reference emissivity values belonging to 15 rock types among sedimentary, igneous and metamorphic categories, which underwent laboratory emissivity estimation by employing a high-sensitivity thermal camera. The results show that rocks can be defined as “emitters”, with emissivity generally ranging from 0.89 to 0.99. Such variability arises from both their intrinsic properties, such as the presence of pores and the different thermal behavior of minerals, and the surface conditions, such as polishing treatments for ornamental stones. The resulting emissivity values are reported and commented on herein for each different studied lithology, thus providing not only a reference dataset for practical use, but also laying the foundation for further scientific studies, also aimed at widening the rock aspects to investigate through IRT.

scholarly journals Blood-Based Detection of Colorectal Cancer Using Cancer-Specific DNA Methylation Markers

Diagnostics ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 51
Author(s):  
Nam-Yun Cho ◽  
Ji-Won Park ◽  
Xianyu Wen ◽  
Yun-Joo Shin ◽  
Jun-Kyu Kang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Sensitivity And Specificity ◽  
Stage Iv ◽  
High Sensitivity ◽  
Liquid Biopsies ◽  
Blood Leukocytes ◽  
Marker Panel ◽  
A Genome ◽  
Methylight Assay

Cancer tissues have characteristic DNA methylation profiles compared with their corresponding normal tissues that can be utilized for cancer diagnosis with liquid biopsy. Using a genome-scale DNA methylation approach, we sought to identify a panel of DNA methylation markers specific for cell-free DNA (cfDNA) from patients with colorectal cancer (CRC). By comparing DNA methylomes between CRC and normal mucosal tissues or blood leukocytes, we identified eight cancer-specific methylated loci (ADGRB1, ANKRD13, FAM123A, GLI3, PCDHG, PPP1R16B, SLIT3, and TMEM90B) and developed a five-marker panel (FAM123A, GLI3, PPP1R16B, SLIT3, and TMEM90B) that detected CRC in liquid biopsies with a high sensitivity and specificity with a droplet digital MethyLight assay. In a set of cfDNA samples from CRC patients (n = 117) and healthy volunteers (n = 60), a panel of five markers on the platform of the droplet digital MethyLight assay detected stages I–III and stage IV CRCs with sensitivities of 45.9% and 95.7%, respectively, and a specificity of 95.0%. The number of detected markers was correlated with the cancer stage, perineural invasion, lymphatic emboli, and venous invasion. Our five-marker panel with the droplet digital MethyLight assay showed a high sensitivity and specificity for the detection of CRC with cfDNA samples from patients with metastatic CRC.

scholarly journals X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples

2021 ◽  
Vol 22 (7) ◽  
pp. 3691
Author(s):  
Oliver Schmutzler ◽  
Sebastian Graf ◽  
Nils Behm ◽  
Wael Y. Mansour ◽  
Florian Blumendorf ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Irradiation Time ◽  
High Sensitivity ◽  
Nanoparticle Uptake ◽  
X Ray ◽  
Functionalized Gold Nanoparticles ◽  
Uptake Measurement ◽  
Prostate Tumor Cells ◽  
Non Destructive

Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.

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