scholarly journals Determination of the Diffusion Coefficient of Urea Solution Using Double Exposure Digital Holographic Interferometry (DEDHI) to Study Plant Growth-=SUP=-*-=/SUP=-

2021 ◽  
Vol 129 (3) ◽  
pp. 371
Author(s):  
Prashant P. Chikode ◽  
Sandip R. Sabale ◽  
Rajiv S. Vhatkar ◽  
Vijay J. Fulari
Keyword(s):  
Diffusion Coefficient ◽  
Plant Growth ◽  
Holographic Interferometry ◽  
Urea Solution ◽  
Distilled Water ◽  
Double Exposure ◽  
Numerical Reconstruction ◽  
Reconstruction Software ◽  
Digital Holograms

In this paper, we have used the double exposure digital holographic interferometry (DEDHI) technique to determine the diffusion coefficient of urea in pure distilled water at 27oC (room temperature) and it is used further to predict urea movement in soil by considering the concentration of urea solution and diffusion coefficient to study early plant growth. We have recorded several consecutive digital holograms of diffusing solutions of urea and pure distilled water at different times instantaneously on the CCD chip and processed them with H-Digital numerical reconstruction software on the computer. It is observed that as the concentration of urea solution increases from 0 to 4 M, diffusion coefficient 'D' of urea slightly increases from 12.2 to 13.8 × 10^-6 cm^2/s, which considerably affects the growth of the plants.

scholarly journals Method for maintenance of coffee leaves in vitro for mass rearing of Leucoptera coffeellum (Guérin-Méneville) (Lepidoptera: Lyonetiidae)

2000 ◽  
Vol 29 (4) ◽  
pp. 849-854 ◽  
Author(s):  
Ronaldo Reis Jr. ◽  
Lima ◽  
Evaldo F. Vilela ◽  
Raimundo S. Barros
Keyword(s):  
Plant Growth ◽  
Mass Rearing ◽  
Developmental Cycle ◽  
Plant Tissues ◽  
Distilled Water ◽  
Complete Development ◽  
Detached Leaves ◽  
Leaf Survival ◽  
The Ideal

To accomplish systematic studies with coffee leafminer, it is necessary to establish a mass rearing system under artificial conditions. It is possible to rear this species, from egg to adult, under laboratory conditions, without using coffee seedlings but detached leaves maintained in vitro. Synthetic cytokinins are routinely used for maintenance of plant cell and plant tissues in vitro. Two plant growth regulators, benzyladenin and kinetin, in concentrations 10-6 and 10-7 M were used to mantain the leaves. Green leaves collected in the field were maintained in the solution to be tested. Distilled water served as control. The experiment lasted 30 days, a period longer than the necessary for the complete development of the insect. Both artificial cytokinines indeed increased the lifetime of the coffee leaves, maintaining them green and healthy. Leaves placed in the cages for oviposition were attractive to the insect, with significant number of eggs per leaf. In most cases, eggs resulted in individuals that completed the whole developmental cycle. Tests with regulator in different concentrations with healthy leaves showed efficiency. However, we believe that hormone concentrations to be used with mined leaves should be larger, because these when maintained at 10-7 M leaves did not present a satisfactory lifetime. Therefore, tests with mined leaves with different hormone concentrations should be made to find out the ideal concentration for leaf survival. In our laboratory we are successfully using 10-6 M benzyladenin for the maintenance of mined leaves.

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