scholarly journals In vitro evaluation of fungicides and some plant extracts against rice sheath rot pathogen Sarocladium oryzae

2016 ◽  
Vol 29 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Shamim Shamsi ◽  
Pranami Chowdhury

Sarocladium oryzae (Sawada) W. Gams & D. Hawksworth, the causal agent of sheath rot of rice was isolated from sheath rot infected rice sheaths and grains. Among ten tested fungicides Tall 25 EC completely inhibited radial growth of the fungus even at the lowest concentration 100 ppm. Similarly ethanol leaf extract of ten plants, namely Allium sativum, Artocarpus heterophyllus, Asparagus racemosus, Azadirachta indica, Citrus medica, Datura metel, Mangifera indica, Nerium indicum, Senna alata and Tagetes erecta at 5, 10 and 20% concentrations were screened for their fungicidal activity against the test fungus. All the plant extracts completely inhibited the radial growth of the test fungus at 20% concentration except Asparagus racemosus and C. medica. Ethanol extract of Tagetes erecta and Mangifera indica also completely inhibited the radial growth of the test fungus at 10% concentration.Bangladesh J. Sci. Res. 29(1): 47-54, June-2016

2018 ◽  
Vol 44 (2) ◽  
pp. 251-259
Author(s):  
Pranami Chowdhury ◽  
MA Bashar ◽  
Shamim Shamsi

Five pathogenic fungi viz., Alternaria alternata (Fr.) Keissler, Curvularia lunata (Wakker) Boedijn, Drechslera oryzae Breda de Haan (Subramanian and Jain), Fusarium moniliforme Sheldon and Pestalotiopsis guepinii (Desm.) Stay. were isolated from two rice varieties viz., BRRI 29 (Boro) and Pajam (Aman) by Blotter and Tissue planting methods. Ten fungicides i.e. Bavistin 50 WP, Salcox 50 WP, Dithane M-45, Indofil M-45, Tall 25 EC, Ridomil MZ Gold, MC Sulphur 80, Greengel, Hayvit 80 WP and Capvit 50 WP at 100, 200, 300, 400 and 500 ppm were evaluated against the above mentioned five pathogenic fungi. Tall 25 EC completely inhibited the radial growth of the test fungi at all the concentrations except Fusarium moniliforme. Antifungal properties of ethanol extract of Artocarpus heterophyllus Lamk., Tagetes erecta L., Datura metel L., Senna alata (L.) Roxb., Azadirachta indica A. Juss., Citrus medica L., Mangifera indica L., Asparagus racemosus Willd., Nerium indicum Mill. and Allium sativum L. at 5, 10 and 20% concentrations were evaluated against the five test pathogens. All the plant extracts completely inhibited the radial growth of the test fungi at 20% concentration except Asparagus racemosus.


2016 ◽  
Vol 42 (1) ◽  
pp. 41-48
Author(s):  
Trisha Saha ◽  
Shamim Shamsi

Anthracnose and blight were recorded on Houttuynia cordata Thunb. during April 2013 to December 2013. The isolated fungi from the symptomatic plants were identified as Alterneria alternata (Fr.) Keissler and Colletotrichum gloeosporoides (Penz.) Sacc. Ethanol leaf extracts of five plants viz.,Azadirachta indica L., Citrus limon L., Datura metel L., Sennaalata L. and Tagetes erecta L.were evaluated against the pathogenic fungi A. alternata and C. gloeosporoides at 5%, 10% and 20% concentrations in vitro. A. indica recorded as good inhibitor against the test fungi followed by C. limon, S. alata, D. metel and T.erecta. In vivo treatment also showed that A.indica is the most effective in controlling diseases at 10% concentration. The plants treated with A. indica were fresh and healthy up to one month of observation.J. Asiat. Soc. Bangladesh, Sci. 42(1): 41-48, June 2016


2020 ◽  
Vol 5 (3) ◽  
pp. 27
Author(s):  
Muhammad Wajid ◽  
Adil Mahmood ◽  
Haseeb Anwar ◽  
Muhammad Imran Hamid ◽  
Yannong Xiao ◽  
...  

There is a vast variety of microbes available in soil performing diverse functions for plants including root colonization for protection against pathogens and nutrient acquisition. In the present study, efficacy of rhizospheric microbial strains and plant extracts were evaluated against citrus nematode for their nematicidal activity. Three different concentrations of plant extracts were evaluated in vitro, results depicts that 10% solution of Tagetes erecta (root) has given 46% the highest percentage mortality at 48hrs followed by Tagetes erecta (leaf) and Datura stramonium killed 41% and 37% nematodes respectively, similarly for 20% of plant extract in distilled water, the highest mortality rate was recorded for T. erecta (root) 68% followed by D. stramonium 47% and Acacia nilotica 44% at 48hrs of application. While using a higher concentration 30% of SS. the percentage mortality elevated multifold i.e.  Marigold root has killed maximum 76% nematodes followed by Marigold leaves and Dhatura with mortality rate 69% and 58% respectively at 48 hrs of interval. While Eucalyptus killed lowest no of nematodes. Additionally, five bacterial isolates were evaluated for their nematicidal activity. Results indicated that for bacterial cell suspension, Pseudomonas putida has shown maximum mortality rate at 84% followed by Bacillus subtilis 73% & Pseudomonas geniculata 65% at 48hrs of interval. Similarly, in the 2nd set of experiment bacterial culture filtrates has exhibited promising results, maximum mortality rate was recorded for Bacillus spp. 81% followed by B. subtilis 76%, Ps putida 63%, after 48hrs of incubation. Moreover, an insight investigation of bacterial extracellular metabolites and plant extracts would enable researchers to develop purposeful products and compositions


2014 ◽  
Vol 23 (1) ◽  
pp. 53-60 ◽  
Author(s):  
MA Bashar ◽  
Mina Chakma

Seven soil fungi viz. Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Penicillium sp., Trichoderma harzianum and T. viride associated with the rhizosphere, non?rhizosphere and rhizoplane of brinjal plants were selected to observe their antagonistic potential against the test fungi Fusarium oxysporum and F. solani . Out of seven soil fungi T. harzianum was found most effective to control the growth of both the test fungi. Plant parts extract of Allium sativum, Asparagus racemosus, Azadirachta indica, Cassia alata, Ocimum sanctum, Zingiber officinale and Datura metel were evaluated for their in vitro efficacy at 5, 10 and 20% concentration against the test fungi. Datura metel, and C. alata and A. indica was found most efficient inhibitor of F. solani and F. oxysporum, respectively. Five fungicides viz., agridazim 50 wp, cozeb 80 wp, newban 50 wp, sunvit 50 wp and vitavax 200 B were evaluated for their in vitro efficacy at 500 ppm concentration against F. solani and F. oxysporum. Vitavax 200B was found most efficient inhibitor of both the test fungi. DOI: http://dx.doi.org/10.3329/dujbs.v23i1.19826 Dhaka Univ. J. Biol. Sci. 23(1): 53-60, 2014


2020 ◽  
Vol 16 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Pavan K. Mujawdiya ◽  
Suman Kapur

Background and Objective: Chronic diseases are associated with low-grade inflammation and oxidative damage. Traditional medicines have been used to manage these disorders due to their high polyphenol content and potent antioxidant activity. We evaluated the in-vitro anti-diabetic and antioxidant potential of extracts of several medicinal plants namely, Mangifera indica, Terminalia arjuna, Moringa oleifera, Albizia lebbeck, Terminalia chebula and Hippophae rhamnoides. Methods: Total polyphenol, flavonoid, and saponin contents were estimated by standard methods. Antioxidant activity was measured using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. The anti-diabetic potential was evaluated using in-vitro α-glucosidase inhibition assay. Results: Terminalia chebula was found to be the richest in both polyphenols (566.5 ± 21.9 μg Gallic acid equivalents/mg of dry weight) and flavonoids (190.67 ± 10.78 quercetin equivalents/mg of dry weight). Extract of Terminalia arjuna was the richest source of saponins (171.92 ± 12.48 μg saponin equivalents/mg of dry weight). All plant extracts showed potent anti-oxidant activity as reflected by their IC50 values in DPPH assay, with Albizia lebbeck (IC50 = 1.35 μg/ml) being the most potent. All plant extracts also showed potent anti-diabetic activity as inferred from their ability to inhibit α- glucosidase, the principal enzyme involved in the metabolism of dietary carbohydrates in the intestine. It was observed that all tested extracts were more potent (IC50 2.53 to 227 μg/ml) in comparison to the standard α-glucosidase inhibitor Acarbose (IC50=2.7 mg/ml). Conclusions: The plant extracts of Mangifera indica, Terminalia arjuna, Moringa oleifera, Albizia lebbeck, Terminalia chebula, and Hippophae rhamnoides possess potent antioxidant and α- glucosidase inhibitory potential and can aid in the management of postprandial hyperglycemia and oxidative damage.


2016 ◽  
Vol 3 (1) ◽  
Author(s):  
MAHESH SINGH ◽  
H. K. SINGH

Six plant extract viz., Datura (Daturastramonium), Eucalyptus (Eucalyptus globulous), Karang (Pongamiaglabra),Neem (Azadirachtaindica), Madar (Calotropis gigantean) and Wild sage (Lantana camara) were evaluated in-vitro by poison food technique @ 3, 6, 9 and 12% concentrations against Alternariabrassicaecausing blight of mustard. The results revealed that all the plant extracts inhibited the percent growth inhibition against test fungus as compared to control. However A.indica was found most efficacious with growth inhibition of (38.7%) followed by E. globulus (36.7%). Some extract such as P. glabra and D. stramonium showed moderate inhibition over control (30.9 and 21.6 respectively). Among all plant extract, C. gigantean showed least (15.3%) inhibition over control against A. brassicae. These plant extracts can possibly be used as management of seedborne pathogenic fungi in an eco-friendly way.


2020 ◽  
Vol 41 (1) ◽  
pp. 51-55
Author(s):  
Suman Prakash Pradhan ◽  
Kapil Adhikari ◽  
Saroj Nepal ◽  
Bishnu Prasad Pandey

Plant extracts contain active ingredients that can absorb, reflect or scatter the sunlight, depending on their nature. The effectiveness of plant extracts as a skin protective agent can be determined by measuring the sun protection factor (SPF). The main aim of this study was to investigate the potential of selected medicinal plant extracts as a component in sunscreen production in modern cosmetics. The absolute methanol extract of six medicinal plants namely; Asparagus racemosus, Bergenia pacumbis, Melia azedarach, Murraya koenigii, Pleurospermum benthamii, and Thymus linearis were examined in-vitro for their sun protective ability by ultraviolet-visible spectrophotometry method with the application of Mansur equation. Our results revealed that most of the plant extracts possess prodigious SPF values as compared with commercial sunscreen. The greatest SPF value was found in P. benthamii (34.97±0.25), T. linearis (24.98±0.60), and B. pacumbis (24.02±0.15). These results show that these plant extracts can act as a very good antisolar agent.


2021 ◽  
Author(s):  
Amina Ifthkar ◽  
Faiza Jabeen ◽  
Saima Mirza ◽  
Afshan Abbas ◽  
Tahira Younis ◽  
...  

Abstract Background: Besides the increasing demand of wooden articles, there is a burning issue of microbial attack which affects the aesthetics of wood. Thus it is a current concern to protect the wooden articles from both fungal and bacterial attack by using toxin free preservatives. Results: Powder of Calotropis procera (leaves), Curcuma zedoaria (rhizome) and Swertia chirata (whole plant) was extracted with solvents like methanol, ethanol and chloroform and then the qualitative phytochemical screening was carried out, which exhibited the presence of different secondary metabolites. These extracts were also evaluated against wood degrading microbes (Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus flavus and Aspergillus niger) isolated from decaying wood. Our study demonstrated that the ethanol extract of S. chairata showed highest bactericidal potential against E.coli (19.0mm) and A. flavus (19.33mm). This study also revealed that S. chirata exhibited strongest biocidal spectrum of action followed by C. procera and C. zedaira. It was observed that laminated veneer treated with ethanol extract of S. chirata showed strong bactericidal potential against E.coli (82.67mm). On the other hand Syzygium cumini wood treated with ethanol extract of C. zedoaria exhibited high antibacterial potential against P. aeruginosa (58.0mm). The active plant extracts showed MIC's ranged from 3.6 to 6.0 mg/ml and MBC/MFC of 4.8 and 8.4 mg/ml. Conclusion: The results demonstrated the potential effects of selected plant extracts as a natural preservative that can protect wood from bacterial and fungal deterioration. The extracts can be applied to prevent furniture deterioration and raw wood protection.


1970 ◽  
Vol 37 (1) ◽  
pp. 85-88 ◽  
Author(s):  
M Yasmin ◽  
KS Hossain ◽  
MA Bashar

Fifty five angiospermic plants were selected for evaluating the effect of their aqueous extracts on the in vitro vegetative growth of Fusarium moniliforme Sheldon. Extracts of 17 plants showed varied degrees of inhibitory effects on the test pathogen. For instance the leaf extract of Lawsonia inermis showed maximum inhibition (60.65 %) followed by roots of Asparagus racemosus (50.59 %). The possibility of using these plant extracts in seed treatment to control bakanae disease of rice is suggested. Antifungal property of leaves of Andrographis paniculata and Lagerstroemia speciosa against bakanae disease is reported here for the first time. Key words: In vitro, Vegetative growth, Bakanae, Fusarium moniliforme, Plant extracts doi:10.3329/bjb.v37i1.1569 Bangladesh J. Bot. 37(1): 85-88, 2008 (June)


Sign in / Sign up

Export Citation Format

Share Document