scholarly journals Evaluación in vitro de fungicidas comerciales para el control de Colletotrichum spp., en mora de castilla

2013 ◽  
Vol 14 (1) ◽  
pp. 67 ◽  
Author(s):  
Viviana Gaviria-Hernández ◽  
Luis Fernando Patiño-Hoyos ◽  
Alegría Saldarriaga-Cardona
Keyword(s):  
Citrus Sinensis ◽  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Acutatum ◽  
Colletotrichum Spp

Se evaluó la eficiencia in vitro de cinco fungicidas de síntesis química, tres extractos vegetales y tres productos a base de biocontroladores, mediante las variables porcentaje de inhibición del crecimiento micelial y porcentaje de inhibición de la biomasa de Colletotrichum gloeosporioides cepa 52 y Colletotrichum acutatum cepa 168. En el grupo de los productos de síntesis química, los mejores resultados en la inhibición del crecimiento micelial en ambas cepas, se obtuvo con hidróxido de cobre y difenoconazol, con 100% de inhibición. En cuanto a inhibición de la biomasa, los productos con mayor porcentaje de efecto inhibitorio en C. gloeosporioides fueron: difenoconazol (100%) y benomil (93% a 99%); en C. acutatum fueron: difenoconazol (100%) y azoxystrobin (91% a 97%). Respecto a los extractos vegetales, el extracto a base de Citrus sinensis y C. grandis presentó 100% de inhibición tanto del crecimiento micelial como de la biomasa, siendo el más efectivo en el control de ambas cepas del hongo. En el grupo de los biocontroladores, el porcentaje de inhibición del crecimiento micelial de los productos a base de Trichoderma lignorum y T. harzianum osciló entre 61% y 65% para C. gloeosporioides, y entre 77% y 79% para C. acutatum, considerados dentro del grupo de los biocontroladores como los más eficientes en el control in vitro de las cepas 52 y 168 de Colletotrichum spp.

scholarly journals Evaluation of Plant-based Pesticide containing Neem Extract (Azadirachta sp.) to Control Anthracnose Growth in Chili Fruits

2021 ◽  
Vol 16 (3) ◽  
pp. 112-122
Author(s):  
Yashanti Berlinda Paradisa ◽  
Wahyuni ◽  
Enung Sri Mulyaningsih ◽  
Ambar Yuswi Perdani ◽  
Arief Heru Prianto
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Acutatum ◽  
Neem Extract ◽  
Colletotrichum Spp

Cabai merupakan salah satu produk hortikultura yang banyak dikonsumsi di Indonesia. Antraknosa merupakan salah satu penyakit utama dalam budi daya cabai. Kehilangan hasil akibat antraknosa mencapai 35%. Penelitian ini bertujuan untuk menentukan efektivitas pestisida nabati dengan bahan utama ekstrak mimba dalam mengendalikan antraknosa pada cabai. Penelitian ini dilakukan di Pusat Penelitian Bioteknologi, LIPI. Pengujian in vitro dilakukan menggunakan rancangan acak kelompok faktorial yang diulang 4 kali dengan faktor pertama ialah cendawan Colletotrichum acutatum dan Colletotrichum gloeosporioides; faktor kedua ialah pestisida nabati dengan bahan aktif mimba (Agr I dan Agr II); serta faktor ketiga berupa 6 taraf perlakuan konsentrasi pestisida (0%, 0.5%, 1%, 2%. 3%, 4%, dan 5%). Pada pengujian in vivo dilakukan menggunakan rancangan acak kelompok faktorial dengan 4 ulangan. Faktor pertama ialah cabai besar dan cabai keriting; faktor kedua ialah C. acutatum dan C. gloeosporioides; dan faktor ketiga ialah 4 taraf konsentrasi pestisida Agr I (0%, 5%, 10, dan 15%). Berdasarkan hasil pengujian diketahui bahwa pestisida nabati Agr I dan Agr II dapat menghambat pertumbuhan cendawan secara in vitro dan Agr I lebih potensial untuk mengendalikan Colletotrichum spp. Namun pestisida nabati Agr I tidak mampu mengendalikan patogen yang telah berada di dalam jaringan tanaman.

scholarly journals The Use of Essential Oils from Thyme, Sage and Peppermint against Colletotrichum acutatum

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 114
Author(s):  
Armina Morkeliūnė ◽  
Neringa Rasiukevičiūtė ◽  
Lina Šernaitė ◽  
Alma Valiuškaitė
Keyword(s):  
Essential Oils ◽  
Fungal Pathogens ◽  
Control Plant ◽  
Antimicrobial Activities ◽  
Plant Diseases ◽  
Colletotrichum Acutatum ◽  
Low Toxicity ◽  
Colletotrichum Spp ◽  
Biological Protection

The Colletotrichum spp. is a significant strawberry pathogen causing yield losses of up to 50%. The most common method to control plant diseases is through the use of chemical fungicides. The findings of plants antimicrobial activities, low toxicity, and biodegradability of essential oils (EO), make them suitable for biological protection against fungal pathogens. The aim is to evaluate the inhibition of Colletotrichum acutatum by thyme, sage, and peppermint EO in vitro on detached strawberry leaves and determine EO chemical composition. Our results revealed that the dominant compound of thyme was thymol 41.35%, peppermint: menthone 44.56%, sage: α,β-thujone 34.45%, and camphor: 20.46%. Thyme EO inhibited C. acutatum completely above 200 μL L−1 concentration in vitro. Peppermint and sage EO reduced mycelial growth of C. acutatum. In addition, in vitro, results are promising for biological control. The detached strawberry leaves experiments showed that disease reduction 4 days after inoculation was 15.8% at 1000 μL L−1 of peppermint EO and 5.3% at 800 μL L−1 of thyme compared with control. Our findings could potentially help to manage C. acutatum; however, the detached strawberry leaves assay showed that EO efficacy was relatively low on tested concentrations and should be increased.

scholarly journals Sensitivity of Colletotrichum Species, Including C. fioriniae and C. nymphaeae, from Peach to Demethylation Inhibitor Fungicides

Plant Disease ◽  
2016 ◽  
Vol 100 (12) ◽  
pp. 2434-2441 ◽  
Author(s):  
S. N. Chen ◽  
C. X. Luo ◽  
M. J. Hu ◽  
G. Schnabel
Keyword(s):  
South Carolina ◽  
Colletotrichum Acutatum ◽  
In Vitro Activity ◽  
Internal Transcribed Spacer Region ◽  
Control Efficacy ◽  
Dmi Fungicides ◽  
Colletotrichum Spp ◽  
Colletotrichum Acutatum Species Complex ◽  
Demethylation Inhibitor

Few fungicides are effective against anthracnose, caused by Colletotrichum spp., and emerging resistance makes the search for chemical alternatives more relevant. Isolates of the Colletotrichum acutatum species complex were collected from South Carolina and Georgia peach orchards and phylogenetic analysis of the combined internal transcribed spacer region, glyceraldehyde-3-phosphate dehydrogenase, and β-tubulin gene sequences separated the isolates into C. nymphaeae and C. fioriniae. The sensitivity of these and three other previously reported Colletotrichum spp. from peach, including C. fructicola, C. siamense, and C. truncatum, to demethylation inhibitor (DMI) fungicides difenoconazole, propiconazole, tebuconazole, metconazole, flutriafol, and fenbuconazole was determined based upon mycelial growth inhibition. C. truncatum was resistant to tebuconazole, metconazole, flutriafol, and fenbuconazole and C. nymphaeae was resistant to flutriafol and fenbuconazole based on 50% effective concentration (EC50) values >100 μg/ml. C. fructicola and C. siamense were sensitive to all DMI fungicides (EC50 values of 0.2 to 13.1 μg/ml). C. fioriniae subgroup 2 isolates were less sensitive to DMI fungicides (EC50 values of 0.5 to 16.2 μg/ml) compared with C. fioriniae subgroup 1 (EC50 values of 0.03 to 2.1 μg/ml). Difenoconazole and propiconazole provided the best control efficacy in vitro to all five species, with EC50 values of 0.2 to 2.7 μg/ml. Tebuconazole and metconazole were effective against all Colletotrichum spp., except for C. truncatum. The strong in vitro activity of some DMI fungicides against Colletotrichum spp. may be exploited for improved anthracnose disease management of peach.

scholarly journals PRODUTOS ALTERNATIVOS NO CONTROLE DE Colletotrichum spp. ISOLADOS DE MANGA E BANANA

2018 ◽  
Vol 5 (3) ◽  
pp. 104-112
Author(s):  
Amanda Cristina De Araújo ◽  
Eliane Divina Toledo ◽  
William Rosa de Oliveira Soares
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Spp ◽  
Colletotrichum Musae

A antracnose causada pelo fungo Colletotrichum spp. ocorre tanto na cultura da manga quanto na cultura da banana, em campo e em pós colheita. A busca pela incorporação de uma nova consciência ecológica vem trazendo conceitos de sustentabilidade para o meio agrícola. Nesse sentido, objetivou-se com este trabalho avaliar o efeito de produtos alternativos em diferentes concentrações sobre o desenvolvimento de Colletotrichum spp. in vitro. Os isolados foram obtidos de frutos com sintomas de antracnose. A atividade antifúngica das concentrações dos tratamentos foi avaliada através da medição em centímetros do crescimento micelial do patógeno. Utilizaram-se cinco concentrações (1, 2, 4, 8, e 16%) dos tratamentos  com essenciais de eucalipto, copaíba , coco, cravo-da-Índia   e alecrim  dos extratos de citronela canela e cravo-da-Índia , do hidrolato de cravo-da-Índia  e do composto majoritário  do óleo de cravo-da-Índia Eugenol  em meio de cultura BDA. O ensaio foi conduzido em dois experimentos com delineamento inteiramente casualizado, em esquema fatorial 10x5 e 7x5 para Colletotrichum musae e Colletotrichum gloeosporioides respectivamente em cada produto utilizado/concentração foram utilizadas quatro repetições. os tratamentos eugenol e óleo de alecrim apresentaram os melhores resultados quanto a inibição do crescimento do fungo em todas concentrações utilizadas. Destaca-se o tratamento com óleo de eucalipto e de cravo que também inibiram totalmente o crescimento de C. musae a partir da concentração 2%. Na avaliação do crescimento de C. gloeosporioides, dentre os sete tratamentos avaliados, apenas o eugenol apresentou potencial total de inibição de crescimento independente da concentração utilizada, seguido do óleo de copaíba que apresentou redução do crescimento em todas as concentrações quando comparado aos demais tratamentos.

In vitro and in vivo activity of QoI fungicides against Colletotrichum gloeosporioides causing fruit anthracnose in Citrus sinensis

2018 ◽  
Vol 236 ◽  
pp. 90-95 ◽  
Author(s):  
Giulio Piccirillo ◽  
Raffaele Carrieri ◽  
Giancarlo Polizzi ◽  
Antonino Azzaro ◽  
Ernesto Lahoz ◽  
...  
Keyword(s):  
Citrus Sinensis ◽  
Colletotrichum Gloeosporioides ◽  
Qoi Fungicides

scholarly journals Synthesis and Characterization of Novel Copper Nanoparticles for the Control of Leaf Spot and Anthracnose Diseases of Olive

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1667
Author(s):  
Panagiota Ntasiou ◽  
Alexandra Kaldeli Kerou ◽  
Theodora Karamanidou ◽  
Afrodite Vlachou ◽  
George T. Tziros ◽  
...  
Keyword(s):  
Leaf Spot ◽  
Mycelial Growth ◽  
Crop Protection ◽  
Attenuated Total Reflection ◽  
Colletotrichum Acutatum ◽  
In Planta ◽  
Colletotrichum Spp ◽  
Type 3

Olive crop is frequently treated with copper fungicides to combat foliar and fruit diseases such as olive leaf spot caused by Fusicladium oleagineum and anthracnose caused by Colletotrichum spp. The replacement of copper-based products with more eco-friendly alternatives is a priority. Metal nanoparticles synthesized in several ways have recently revolutionized crop protection with applications against important crop pathogens. In this study, we present the development of four copper-based nanoparticles (CuNP Type 1 to 4) synthesized with a wet chemistry approach. The CuNPs were characterized using Transmission Electron Microscopy, Dynamic Light Scattering, Laser Doppler Electrophoresis, and Attenuated Total Reflection measurements. In addition, the activity of the four CuNP types was tested in vitro and in planta against F. oleagineum and Colletotrichum spp. In vitro sensitivity measurements showed that for both pathogens, mycelial growth was the most susceptible developmental stage to the tested compounds. Against both pathogens, CuNP Type 1 and Type 2 were found to be more active in reducing mycelial growth compared to the reference commercial compounds of copper oxide and copper hydroxide. In planta experiments showed that CuNP Type 3 and CuNP Type 4 exhibited a strong protectant activity against both F. oleagineum and Colletotrichum acutatum with control efficacy values significantly higher than those achieved by the applications of either reference product.

scholarly journals Evaluación in vitro del quitosano y aceites esenciales para el control de dos especies patógenas de Colletotrichum aisladas de aguacate (Persea americana Mill)

2019 ◽  
Vol 22 ◽  
Author(s):  
Mireya Esbeiddy Chávez-Magdaleno ◽  
Porfirio Gutiérrez-Martínez ◽  
Beatriz Montaño-Leyva ◽  
Ramsés Ramón González-Estrada
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Persea Americana ◽  
Colletotrichum Acutatum

La actividad antifúngica del quitosano, aceites esenciales (canela y eucalipto) y su combinación fueron evaluadas contra dos patógenos Colletotrichum acutatum y Colletotrichum gloeosporioides aislados de aguacate (Persea americana Mill). Se utilizó quitosano a diferentes concentraciones (0.1, 0.5 y 1.0%). Las concentraciones de aceites esenciales evaluadas fueron 0.1, 0.5 y 2.0% (eucalipto y canela). El efecto de la combinación del quitosano con los aceites esenciales se evaluó utilizando las concentraciones más eficaces del quitosano (0.1 y 0.5%) y el aceite esencial de eucalipto y canela con el mayor efecto inhibitorio (2%). Los tratamientos de quitosano y aceites esenciales cada uno por separado fueron parcialmente efectivos para evitar el desarrollo de las cepas evaluadas, inhibiendo el crecimiento micelial en un rango de 17 a 21% y 18 a 50% respectivamente. Por lo contrario, el crecimiento micelial de ambas cepas fue totalmente reducido mediante la aplicación combinada del quitosano (0.1%) con aceite esencial de canela (2%), sin embargo; la combinación del quitosano con aceite esencial de eucalipto no fue efectiva. Hasta donde sabemos, esta investigación es la primera que informa sobre cepas de Colletotrichum resistentes al quitosano aisladas de frutos de aguacate.

scholarly journals Oversummer Survival of Inoculum for Colletotrichum Crown Rot in Buried Strawberry Crown Tissue

Plant Disease ◽  
2001 ◽  
Vol 85 (7) ◽  
pp. 750-754 ◽  
Author(s):  
A. R. Ureña-Padilla ◽  
D. J. Mitchell ◽  
D. E. Legard
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Crown Rot ◽  
Colletotrichum Acutatum ◽  
Initial Increase ◽  
Spore Morphology ◽  
Plant Debris ◽  
Crown Tissue ◽  
Colletotrichum Spp ◽  
Species Specific ◽  
Dna Primers

The oversummer survival of Colletotrichum gloeosporioides in strawberry crown tissue under field conditions was investigated in 1998 and 1999. Strawberry crowns infected naturally with C. gloeosporioides were placed inside cloth bags containing field soil, buried in the field at 5 or 13 cm, then recovered over 6 months of each year. The recovered crowns were plated onto a Colletotrichum spp. semiselective medium and speciated by colony, spore morphology, and molecular markers with species-specific DNA primers. Pathogenicity of selected isolates was confirmed by greenhouse bioassays on strawberry. Of the 428 isolates of Colletotrichum spp. recovered from buried crowns, 96% were C. gloeosporioides and 4% Colletotrichum acutatum. Following an initial increase in the detection of the fungus, survival of C. gloeosporioides was stable for 2 to 3 weeks, then declined. No Colletotrichum spp. were detected after burial for 56 days in 1998 and 98 days in 1999. Because the time between crop seasons is typically more than 170 days, these data support the hypothesis that inoculum of C. gloeosporioides does not survive in buried plant debris between seasons in Florida and, therefore, oversummering crop debris does not contribute inoculum for epidemics of Colletotrichum crown rot in Florida.

scholarly journals Differential Reactions on Mature Green and Ripe Chili Fruit Infected by Three Colletotrichum spp.

Plant Disease ◽  
2010 ◽  
Vol 94 (3) ◽  
pp. 306-310 ◽  
Author(s):  
Orarat Mongkolporn ◽  
Paweena Montri ◽  
Thunyawan Supakaew ◽  
Paul W. J. Taylor
Keyword(s):  
Capsicum Annuum ◽  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Acutatum ◽  
Ripe Fruit ◽  
Green Fruit ◽  
Fruit Maturity ◽  
Capsicum Spp ◽  
Colletotrichum Spp ◽  
Differential Hosts ◽  
Fruit Stage

Differential reactions on mature green and ripe chili fruit of 10 genotypes from four cultivated Capsicum spp. (i.e., Capsicum annuum, C. baccatum, C. chinense, and C. frutescens) were investigated after being inoculated with 33 isolates of Colletotrichum capsici, C. gloeosporioides, and C. acutatum originating from Thailand. The inoculation was performed using an injection method. Differential reactions, based on qualitative host reactions (i.e., lesion development versus no infection) grouped the Colletotrichum isolates into different pathotypes. C. capsici was grouped into three pathotypes based on differential reactions on ripe fruit stage of two Capsicum chinense genotypes (PBC932 and C04714) and two pathotypes based on differential reactions on mature green fruit stage of C04714. Colletotrichum gloeosporioides was grouped into five and six pathotypes on their reactions in ripe and green fruit maturity stages, respectively. C04714 and two Capsicum annuum genotypes (Jinda and Bangchang) acted as differential hosts with ripe fruit, whereas C04714 with all C. annuum and C. frutescens genotypes acted as differential hosts with green fruit. No pathotype of Colletotrichum acutatum was identified on ripe fruit but three pathotypes were identified on green fruit based on differential reactions in two Capsicum baccatum genotypes (PBC80 and PBC81).

scholarly journals Chemical composition and in vitroantifungal activity of turmeric oilfrom Curcuma longa L. rhizomeagainst Colletotrichum spp.

2021 ◽  
Vol 63 (6) ◽  
pp. 6-10
Author(s):  
Trung Hieu Pham ◽  
◽  
Dai Lam Tran ◽  
Thi Nam Pham ◽  
Dinh Hoang Vu ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Colletotrichum Gloeosporioides ◽  
Curcuma Longa ◽  
Inhibitory Effect ◽  
Volatile Components ◽  
In Vitro Growth ◽  
Strong Inhibition ◽  
Colletotrichum Spp ◽  
Poisoned Food Technique

The production of curcumin produces large quantities of the spent turmeric oleoresin as a by-product, which often pollutes the environment. This study was conducted to obtain turmeric oil from this by-product and evaluate its antifungal activity. Turmeric oil afforded by partitioning the turmeric oleoresin from the production of curcumin (Curcuma longa L.) with n-hexane showed a strong inhibition for Colletotrichum species. The volatile components of turmeric oil were determined by GC-MS. Then, 23 volatile compounds were identified from this oil, of which four main compounds were α-zingiberene, α-turmerone, ar-turmerone, and β-sesquiphellandrene. Besides, the authors also isolated Colletotrichum gloeosporioides from lychee fruits, which were harvested in Luc Ngan district, Bac Giang province. By poisoned food technique, the antifungal activity of turmeric oil against C. gloeosporioides, C. orbiculare, C. acutatum, Phytophthora infestans, and Fusarium oxysporum was demonstrated. The results showed that at a concentration of 1 mg/ml, turmeric oil showed the highest inhibitory effect on C. gloeosporioides (67.9%). As for other fungi, in vitro growth inhibition varied in the range of 41-62%.

Sign in / Sign up

Export Citation Format

Share Document