scholarly journals Prevention of cervical cancer development through early detection of HPV using novelty molecular applications

Bionatura ◽  
2019 ◽  
Vol 02 (Bionatura Conference Serie) ◽  
Author(s):  
Kerly León-Ordóñez ◽  
Sofía Abad-Sojos
Keyword(s):  
Cervical Cancer ◽  
Early Detection ◽  
High Sensitivity ◽  
Hpv Infection ◽  
Molecular Techniques ◽  
Cancer Development ◽  
Viral Oncoproteins ◽  
Hpv E6 ◽  
E6 And E7 ◽  
Cervical Cancer Development

Human Papillomavirus (HPV) is the predominant cause of cervical cancer worldwide. The infections with HPVs 16 and 18 have a high oncogenic risk for cancer development. Besides, the genes E6 and E7 encode viral oncoproteins associated with infection. New molecular techniques for HPV detection, show important advantages such as high sensitivity, recognition capacity, reliability, among others. These techniques allow the standardization of new protocols associated with the detection in a variety of substances and samples. The stretch relationship between the virus and the disease open a new field to study early detection of the HPV infection. Additionally, less concentration of the sample is needed. Considering the significance of the detection, the present paper explains five novelty molecular applications for the prevention cervical cancer and early detection of HPV such as RNA in situ Hybridization for the detection of HPV E6/E7, genosensors, electrochemical DNA biosensor, PCR-based urine assay and a semen assay for detection of HPV. All the methods related to DNA samples could be used for both genders, there are more acceptable and easy to collect.

scholarly journals Role of inflammasome genetics in susceptibility to HPV infection and cervical cancer development

2016 ◽  
Vol 88 (9) ◽  
pp. 1646-1651 ◽  
Author(s):  
A. Pontillo ◽  
P. Bricher ◽  
V.N.C. Leal ◽  
S. Lima ◽  
P.R.E. Souza ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hpv Infection ◽  
Cancer Development ◽  
Cervical Cancer Development

scholarly journals Significance of molecular diagnostics in human papilloma virus (HPV) determination

2014 ◽  
Vol 66 (1) ◽  
pp. 51-56 ◽  
Author(s):  
G. Kovacevic ◽  
V. Milosevic ◽  
I. Hrnjakovic-Cvjetkovic ◽  
V. Petrovic ◽  
S. Stefan-Mikic ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hpv Infection ◽  
Molecular Techniques ◽  
Etiologic Factor ◽  
Abnormal Cytology ◽  
Normal Cytology ◽  
Papilloma Virus ◽  
Cervical Cancer Development ◽  
High Risk Hpv

HPV infection is considered to be the most important etiologic factor in cervical cancer development. In this retrospective study, which included the period from 2000 to 2012, the results of two molecular techniques used in the detection of HPV infection among women of the South Backa District were analyzed. By using the technique of in situ hybridization and the rPCR method, the proportion of high-risk HPV among women with normal cytology was determined to be 19.8% and 32.7%, respectively, and among women with abnormal cytology 43.1% and 61%, respectively. Among the analyzed women, HPV type 16 was the most prevalent, followed by HPV types 31, 51 and 18. Application of molecular HPV diagnosis is valuable because it increases the sensitivity of the screening test, so that the application of both tests to detect cervical cancer is a true prevention of malignancy.

scholarly journals The Preventive Effect of Dietary Antioxidants on Cervical Cancer Development

Medicina ◽  
2020 ◽  
Vol 56 (11) ◽  
pp. 604
Author(s):  
Ayumi Ono ◽  
Masafumi Koshiyama ◽  
Miwa Nakagawa ◽  
Yumiko Watanabe ◽  
Eri Ikuta ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Nutrient Intake ◽  
Continuous Process ◽  
Relevant Literature ◽  
Hpv Infection ◽  
Cancer Development ◽  
Dietary Antioxidants ◽  
Cervical Cancer Development

Cervical cancer results from a continuous process, starting from a normal cervical epithelium after human papillomavirus (HPV) infection and progressing to cervical intraepithelial neoplasia (CIN), before finally developing into invasive squamous carcinoma (ISC). In recent decades, dietary antioxidants, such as vitamins, have received much attention in relation to cancer prevention. We reviewed the relevant literature to investigate the dietary and nutrient intake on cervical cancer. The intake of vitamins A and D and carotenoids may inhibit early cervical cancer development. The intake of folate may prevent or inhibit HPV infection rom progressing to various grades of CIN. The intake of vitamins C and E may widely inhibit the process of cervical cancer development. Polyphenols are often used in cases of cervical cancer in combination chemotherapy and radiation therapy. Regarding nutrients, different antioxidants may have differing abilities to intervene in the natural history of cervical diseases associated with HPV infection. Regarding foods, the intake of both vegetables and fruits containing multiple vitamins may widely suppress cervical cancer development. Most previous papers have described epidemiological studies. Thus, further research using in vitro and in vivo approaches will be needed to clarify the effects of the dietary and nutrient intake in detail.

scholarly journals The function of histone acetylation in cervical cancer development

2019 ◽  
Vol 39 (4) ◽  
Author(s):  
Shanshan Liu ◽  
Weiqin Chang ◽  
Yuemei Jin ◽  
Chunyang Feng ◽  
Shuying Wu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Histone Acetylation ◽  
Gene Sequence ◽  
Hpv Infection ◽  
Cancer Development ◽  
Post Translational Modifications ◽  
Cellular Life ◽  
Lysine Residues ◽  
Cervical Cancer Development ◽  
Gene Expression Levels

AbstractCervical cancer is the fourth most common female cancer in the world. It is well known that cervical cancer is closely related to high-risk human papillomavirus (HPV) infection. However, epigenetics has increasingly been recognized for its role in tumorigenesis. Epigenetics refers to changes in gene expression levels based on non-gene sequence changes, primarily through transcription or translation of genes regulation, thus affecting its function and characteristics. Typical post-translational modifications (PTMs) include acetylation, propionylation, butyrylation, malonylation and succinylation, among which the acetylation modification of lysine sites has been studied more clearly so far. The acetylation modification of lysine residues in proteins is involved in many aspects of cellular life activities, including carbon metabolism, transcriptional regulation, amino acid metabolism and so on. In this review, we summarize the latest discoveries on cervical cancer development arising from the aspect of acetylation, especially histone acetylation.

scholarly journals Human papillomavirus and other risk factors in Lithuanian cervical carcinoma patients

2010 ◽  
Vol 17 (3-4) ◽  
pp. 106-115
Author(s):  
Živilė GUDLEVIČIENĖ ◽  
Alė SMILGEVIČIŪTĖ-IVSHIN ◽  
Aurelija VAITKUVIENĖ ◽  
Agnė ŠEPETIENĖ ◽  
Janina DIDŽIAPETRIENĖ
Keyword(s):  
Risk Factors ◽  
Cervical Cancer ◽  
Cancer Risk ◽  
Hpv Infection ◽  
Cancer Development ◽  
Cancer Risk Factors ◽  
Cervical Cancer Risk ◽  
Hpv Prevalence ◽  
Geographical Regions ◽  
Cervical Cancer Development

Background. Cervical cancer is the second biggest cause of female cancer mortality worldwide and the most common cancer in women in Lithuania. The incidence of cervical cancer is strongly associated with HPV prevalence. However, not only HPV infection plays a crucial role in cervical cancer development; other risk factors which vary in different populations and geographical regions as well as HPV prevalence are important. The aim of this study was to detect the HPV, its type’s prevalence and other cervical cancer risk factors for Lithuanian women. Materials and methods. 191 women with primary diagnosed invasive cervical cancer (cases group) and 397 control women were invited to participate in the study. All women were interviewed and samples for HPV testing were taken. Results. In the cases group, 92.7% of women and in the control group 26.7% were infected by HPV (p < 0.0001). HPV 16 was the most common type in both groups. HPV infection increases the risk of cervical cancer 75 times (OR = 75.39; 95% CI 33.61–192.98). Women with lower education, workers, those who started sexual intercourses before 20 years of age, at older age of the first menstrual period, 3–5 or more childbirths, smokers and with a long ago or never performed Pap test are at a significantly higher risk of cervical cancer development. Other non-HPV risk factors were not associated with cervical cancer risk. Conclusions. Data of our study show a high prevalence of HPV in Lithuanian population. It may have an impact on the biggest cervical cancer incidence. The other risk factors are similar as in other lower economic resource countries. Keywords: HPV, cervical cancer, risk factors

scholarly journals miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Joana Ribeiro ◽  
Joana Marinho-Dias ◽  
Paula Monteiro ◽  
Joana Loureiro ◽  
Inês Baldaque ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Expression Analysis ◽  
Reference Group ◽  
Invasive Cervical Cancer ◽  
Hpv Infection ◽  
Cancer Development ◽  
Cervical Lesions ◽  
Normal Epithelium ◽  
Normal Cervix ◽  
Cervical Cancer Development

We aimed to characterize miR-125b and miR-34a expression in 114 women with different cervical lesions: normal epithelium with (n= 20) and without (n= 29) HPV infection; LSIL (n= 28); HSIL (n= 29); and ICC (n= 8). miRNA expression analysis was performed by comparing the distinct groups with the reference group (women with normal epithelium without HPV). For miR-125b, we observed a twofold (2-ΔΔCt= 2.11;P= 0.038) increased expression among women with normal epithelium with HPV infection and a trend of downregulation in different cervical lesions including an 80% reduction (2-ΔΔCt= 0.21;P= 0.004) in ICC. Similarly, miR-34a expression analysis revealed an increased expression (2-ΔΔCt= 1.69;P= 0.049) among women with normal cervix and HPV infection, and despite no significant correlation with cervical lesions, its expression was increased by twofold (2-ΔΔCt= 2.08;P= 0.042) in ICC. Moreover, miR-125b levels were able to predict invasive cancers with 88% sensitivity and 69% specificity. Results showed that while miR-34a expression seems to be correlated with invasive cervical cancer, miR-125b expression is significantly changed within the different cervical lesions and their levels should be further investigated as possible predictive/prognostic biomarkers using a noninvasive approach.

miR-122 Inhibits the Cervical Cancer Development by Targeting the Oncogene RAD21

2021 ◽  
Author(s):  
Yanling Yang ◽  
Yang Liu ◽  
Wei Liu ◽  
Chunyang Li ◽  
Yuan Liu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cancer Development ◽  
Cervical Cancer Development

scholarly journals Genetic polymorphisms and cervical cancer development: ATM G5557A and p53bp1 C1236G

2011 ◽  
Vol 27 (4) ◽  
pp. 1188-1192 ◽  
Author(s):  
S. OLIVEIRA ◽  
J. RIBEIRO ◽  
H. SOUSA ◽  
D. PINTO ◽  
I. BALDAQUE ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Genetic Polymorphisms ◽  
Cancer Development ◽  
Cervical Cancer Development

scholarly journals Human Papillomavirus 16 Oncoproteins Downregulate the Expression of miR-148a-3p, miR-190a-5p, and miR-199b-5p in Cervical Cancer

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Mi-Soon Han ◽  
Jae Myun Lee ◽  
Soo-Nyung Kim ◽  
Jae-Hoon Kim ◽  
Hyon-Suk Kim
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
High Risk ◽  
Expression Profiles ◽  
Normal Group ◽  
Cancer Development ◽  
Hybridization Method ◽  
Cancer Group ◽  
Cervical Cancer Development ◽  
High Risk Hpv

Almost all cervical cancers are associated with human papillomavirus (HPV); however, the majority of women infected with this virus do not develop cervical cancer. Therefore, new markers are needed for reliable screening of cervical cancer, especially in relation to HPV infection. We aimed to identify potential microRNAs that may serve as diagnostic markers for cervical cancer development in high-risk HPV-positive patients. We evaluated the microRNA expression profiles in 12 cervical tissues using the hybridization method and verified them by quantitative polymerase chain reaction (qPCR). Finally, we evaluated the effects of HPV16 oncoproteins on the expression of selected microRNAs using cervical cancer cells (CaSki and SiHa) and RNA interference. With the hybridization method, eight microRNAs (miR-9-5p, miR-136-5p, miR-148a-3p, miR-190a-5p, miR-199b-5p, miR-382-5p, miR-597-5p, and miR-655-3p) were found to be expressed differently in the HPV16-positive cervical cancer group and HPV16-positive normal group (fold change ≥ 2). The results of qPCR showed that miR-148a-3p, miR-190a-5p, miR-199b-5p, and miR-655-3p levels significantly decreased in the cancer group compared with the normal group. Upon silencing of HPV16 E5 and E6/E7, miR-148a-3p levels increased in both cell lines. Silencing of E6/E7 in SiHa cells led to the increase in miR-199b-5p and miR-190a-5p levels. Three HPV16 oncoproteins (E5, E6, and E7) downregulate miR-148a-3p, while E6/E7 inhibit miR-199b-5p and miR-190a-5p expression in cervical carcinoma. The three microRNAs, miR-148a-3p, miR-199b-5p, and miR-190a-5p, may be novel diagnostic biomarkers for cervical cancer development in high-risk HPV-positive patients.

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