miR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development

We aimed to characterize miR-125b and miR-34a expression in 114 women with different cervical lesions: normal epithelium with (n= 20) and without (n= 29) HPV infection; LSIL (n= 28); HSIL (n= 29); and ICC (n= 8). miRNA expression analysis was performed by comparing the distinct groups with the reference group (women with normal epithelium without HPV). For miR-125b, we observed a twofold (2-ΔΔCt= 2.11;P= 0.038) increased expression among women with normal epithelium with HPV infection and a trend of downregulation in different cervical lesions including an 80% reduction (2-ΔΔCt= 0.21;P= 0.004) in ICC. Similarly, miR-34a expression analysis revealed an increased expression (2-ΔΔCt= 1.69;P= 0.049) among women with normal cervix and HPV infection, and despite no significant correlation with cervical lesions, its expression was increased by twofold (2-ΔΔCt= 2.08;P= 0.042) in ICC. Moreover, miR-125b levels were able to predict invasive cancers with 88% sensitivity and 69% specificity. Results showed that while miR-34a expression seems to be correlated with invasive cervical cancer, miR-125b expression is significantly changed within the different cervical lesions and their levels should be further investigated as possible predictive/prognostic biomarkers using a noninvasive approach.

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Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions

Viruses ◽

10.3390/v11040350 ◽

2019 ◽

Vol 11 (4) ◽

pp. 350 ◽

Cited By ~ 1

Author(s):

Yusuke Hirose ◽

Mamiko Onuki ◽

Yuri Tenjimbayashi ◽

Mayuko Yamaguchi-Naka ◽

Seiichiro Mori ◽

...

Keyword(s):

Cervical Cancer ◽

Large Scale ◽

Phylogenetic Analyses ◽

Invasive Cervical Cancer ◽

Japanese Women ◽

Genetic Variations ◽

Human Papillomaviruses ◽

Cancer Development ◽

Cervical Lesions ◽

Cervical Cancer Development

Recent large-scale genomics studies of human papillomaviruses (HPVs) have shown a high level of genomic variability of HPV16, the most prevalent genotype in HPV-associated malignancies, and provided new insights into the biological and clinical relevance of its genetic variations in cervical cancer development. Here, we performed deep sequencing analyses of the viral genome to explore genetic variations of HPV16 that are prevalent in Japan. A total of 100 complete genome sequences of HPV16 were determined from cervical specimens collected from Japanese women with cervical intraepithelial neoplasia and invasive cervical cancer, or without cervical malignancies. Phylogenetic analyses revealed the variant distribution in the Japanese HPV16 isolates; overall, lineage A was the most prevalent (94.0%), in which sublineage A4 was dominant (52.0%), followed by sublineage A1 (21.0%). The relative risk of sublineage A4 for cervical cancer development was significantly higher compared to sublineages A1/A2/A3 (odds ratio = 6.72, 95% confidence interval = 1.78–28.9). Interestingly, a novel cluster of variants that branched from A1/A2/A3 was observed for the Japanese HPV16 isolates, indicating that unique HPV16 variants are prevalent among Japanese women.

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Role of inflammasome genetics in susceptibility to HPV infection and cervical cancer development

Journal of Medical Virology ◽

10.1002/jmv.24514 ◽

2016 ◽

Vol 88 (9) ◽

pp. 1646-1651 ◽

Cited By ~ 28

Author(s):

A. Pontillo ◽

P. Bricher ◽

V.N.C. Leal ◽

S. Lima ◽

P.R.E. Souza ◽

...

Keyword(s):

Cervical Cancer ◽

Hpv Infection ◽

Cancer Development ◽

Cervical Cancer Development

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microRNA Levels in Cervical Cancer Samples and Relationship with Lesion Grade and HPV Infection

MicroRNA ◽

10.2174/2211536610666210604123534 ◽

2021 ◽

Vol 10 ◽

Author(s):

Carolina R. Hoelzle ◽

Solène Arnoult ◽

Cinthya R.M. Borém ◽

Mariana Ottone ◽

Kênia C.S.F. de Magalhães ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Progression ◽

Invasive Cervical Cancer ◽

Rna Extraction ◽

Hpv Infection ◽

Control Group ◽

Plasma Samples ◽

Cervical Lesions ◽

Dna And Rna ◽

Cervical Scraping

Background: miR-21, miR-214, and miR-let-7a are three validated and well-known miRNAs. miR-21 is described as an “oncomir,” while miR-214 and miR-let-7a are described mainly as tumor suppressors. The role of these miRNAs remains unclear in cervical cancer, an important malignancy among women worldwide and responsible for many deaths every year. Objective: The objective of this study was to describe the expression profile of miR-21, miR-214, and miR-let-7a in plasma and cervical scraping from a control group and patients with different grades of cervical lesions and invasive cervical cancer, and then correlate with HPV infection groups. Methods: Plasma and cervical scraping were submitted to DNA and RNA extraction. HPV detection and typing were performed by conventional PCR followed by PAGE to amplicons interpretation. The miRNA relative expression in plasma and cervical scraping samples was performed by real-time PCR using specific TaqMan probes. Results: miR-21 (p=0.0277) and miR-214 (p=0.0151) were up-regulated in cervical scraping samples of the invasive cervical cancer (ICC) group. However, miR-214 was also up-regulated in the LSIL group (p=0.0062). Both miRNAs were not related to HPV infection. However, miR-let-7a was higher in HPV positive plasma samples (p=0.0433) than in HPV negative plasma samples, and the correlation analysis confirmed the association between the levels of this miRNA with the presence of HPV (p=0.0407; r=0.3029), but not with lesion grade (p>0.05). Conclusion: Our results suggest that miR-21 is related to cervical cancer progression and miR-214 appears to have an ambiguous role in cervical lesions. miR-let-7a may be upregulated at the systemic level in patients with HPV infection.

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The Preventive Effect of Dietary Antioxidants on Cervical Cancer Development

Medicina ◽

10.3390/medicina56110604 ◽

2020 ◽

Vol 56 (11) ◽

pp. 604

Author(s):

Ayumi Ono ◽

Masafumi Koshiyama ◽

Miwa Nakagawa ◽

Yumiko Watanabe ◽

Eri Ikuta ◽

...

Keyword(s):

Cervical Cancer ◽

Nutrient Intake ◽

Continuous Process ◽

Relevant Literature ◽

Hpv Infection ◽

Cancer Development ◽

Dietary Antioxidants ◽

Cervical Cancer Development

Cervical cancer results from a continuous process, starting from a normal cervical epithelium after human papillomavirus (HPV) infection and progressing to cervical intraepithelial neoplasia (CIN), before finally developing into invasive squamous carcinoma (ISC). In recent decades, dietary antioxidants, such as vitamins, have received much attention in relation to cancer prevention. We reviewed the relevant literature to investigate the dietary and nutrient intake on cervical cancer. The intake of vitamins A and D and carotenoids may inhibit early cervical cancer development. The intake of folate may prevent or inhibit HPV infection rom progressing to various grades of CIN. The intake of vitamins C and E may widely inhibit the process of cervical cancer development. Polyphenols are often used in cases of cervical cancer in combination chemotherapy and radiation therapy. Regarding nutrients, different antioxidants may have differing abilities to intervene in the natural history of cervical diseases associated with HPV infection. Regarding foods, the intake of both vegetables and fruits containing multiple vitamins may widely suppress cervical cancer development. Most previous papers have described epidemiological studies. Thus, further research using in vitro and in vivo approaches will be needed to clarify the effects of the dietary and nutrient intake in detail.

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The function of histone acetylation in cervical cancer development

Bioscience Reports ◽

10.1042/bsr20190527 ◽

2019 ◽

Vol 39 (4) ◽

Cited By ~ 2

Author(s):

Shanshan Liu ◽

Weiqin Chang ◽

Yuemei Jin ◽

Chunyang Feng ◽

Shuying Wu ◽

...

Keyword(s):

Cervical Cancer ◽

Histone Acetylation ◽

Gene Sequence ◽

Hpv Infection ◽

Cancer Development ◽

Post Translational Modifications ◽

Cellular Life ◽

Lysine Residues ◽

Cervical Cancer Development ◽

Gene Expression Levels

AbstractCervical cancer is the fourth most common female cancer in the world. It is well known that cervical cancer is closely related to high-risk human papillomavirus (HPV) infection. However, epigenetics has increasingly been recognized for its role in tumorigenesis. Epigenetics refers to changes in gene expression levels based on non-gene sequence changes, primarily through transcription or translation of genes regulation, thus affecting its function and characteristics. Typical post-translational modifications (PTMs) include acetylation, propionylation, butyrylation, malonylation and succinylation, among which the acetylation modification of lysine sites has been studied more clearly so far. The acetylation modification of lysine residues in proteins is involved in many aspects of cellular life activities, including carbon metabolism, transcriptional regulation, amino acid metabolism and so on. In this review, we summarize the latest discoveries on cervical cancer development arising from the aspect of acetylation, especially histone acetylation.

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TPP1 Regulates hTERT Expression and Predicts Early Malignant Event and Prognosis of Cervical Cancer

10.21203/rs.3.rs-794147/v1 ◽

2021 ◽

Author(s):

Qiaoli Wang ◽

Caifeng Gong ◽

Hui Yang ◽

Fuxiang Zhou ◽

Qiuji Wu ◽

...

Keyword(s):

Cervical Cancer ◽

Protein Expression ◽

Binding Protein ◽

High Expression ◽

Cancer Development ◽

Htert Expression ◽

Telomere Binding Protein ◽

Normal Cervix ◽

Cervical Cancer Development

Abstract Background: Cervical cancer is one of the most common deadly cancer in women worldwide. However, identifying specific biomarkers is still needed. Telomere-binding protein 1 (TPP1) is vital to telomerase activity. However, the role of TPP1 in cervical cancer and its association with human telomerase reverse transcriptase (hTERT) is unclear.This study aimed at exploring the role of telomere-binding protein 1 (TPP1) in cervical cancer development and progression, and potential mechanisms.Methods: Tissue samples from a total of 274 participants were enrolled for the evaluation of protein expression,156 of whom diagnosed withcervical cancers, 102 with cervical intraepithelial neoplasia (CIN) and 16 with normal cervix. In addition, in vitro cellular models with cervical cancer cell lines Hela, Siha, and C33a were transfected by TPP1-siRNAand protein expression of TPP1 and hTERT were assessed. Results: Compared with normal cervix, TPP1 expression was significantly higher in CIN-III and cervical cancers (P<0.001 for both). High expression of TPP1alone (Plog-rank=0.047)andhigh co-expression of TPP1/hTERT (Plog-rank=0.005)weresignificantly associated with worse survival of cervical cancer patients.After adjusting for well-known prognosis factors, hazard ratio was 2.03(95% confidence interval [CI] 0.99-4.16)for high expression of TPP1 and 2.01(95% CI 1.10-3.67) for high co-expression of TPP1/hTERT. TPP1 and hTERT expressions were positively correlated atall levels of cervical lesions (r=0.524, P<0.001). Knockdown of TPP1 decreased hTERT mRNA and protein expression.Conclusions: High expression of TPP1 might be an early event during cervical cancer development and could be served as apotential prognosis biomarker, especially when used together with hTERT. TPP1 might regulate hTERT expression with detailed underlying mechanisms warrant further investigation.

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Human papillomavirus and other risk factors in Lithuanian cervical carcinoma patients

Acta medica Lituanica ◽

10.15388/amed.2010.21683 ◽

2010 ◽

Vol 17 (3-4) ◽

pp. 106-115

Author(s):

Živilė GUDLEVIČIENĖ ◽

Alė SMILGEVIČIŪTĖ-IVSHIN ◽

Aurelija VAITKUVIENĖ ◽

Agnė ŠEPETIENĖ ◽

Janina DIDŽIAPETRIENĖ

Keyword(s):

Risk Factors ◽

Cervical Cancer ◽

Cancer Risk ◽

Hpv Infection ◽

Cancer Development ◽

Cancer Risk Factors ◽

Cervical Cancer Risk ◽

Hpv Prevalence ◽

Geographical Regions ◽

Cervical Cancer Development

Background. Cervical cancer is the second biggest cause of female cancer mortality worldwide and the most common cancer in women in Lithuania. The incidence of cervical cancer is strongly associated with HPV prevalence. However, not only HPV infection plays a crucial role in cervical cancer development; other risk factors which vary in different populations and geographical regions as well as HPV prevalence are important. The aim of this study was to detect the HPV, its type’s prevalence and other cervical cancer risk factors for Lithuanian women. Materials and methods. 191 women with primary diagnosed invasive cervical cancer (cases group) and 397 control women were invited to participate in the study. All women were interviewed and samples for HPV testing were taken. Results. In the cases group, 92.7% of women and in the control group 26.7% were infected by HPV (p < 0.0001). HPV 16 was the most common type in both groups. HPV infection increases the risk of cervical cancer 75 times (OR = 75.39; 95% CI 33.61–192.98). Women with lower education, workers, those who started sexual intercourses before 20 years of age, at older age of the first menstrual period, 3–5 or more childbirths, smokers and with a long ago or never performed Pap test are at a significantly higher risk of cervical cancer development. Other non-HPV risk factors were not associated with cervical cancer risk. Conclusions. Data of our study show a high prevalence of HPV in Lithuanian population. It may have an impact on the biggest cervical cancer incidence. The other risk factors are similar as in other lower economic resource countries. Keywords: HPV, cervical cancer, risk factors

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Prevention of cervical cancer development through early detection of HPV using novelty molecular applications

Bionatura ◽

10.21931/rb/cs/2019.02.01.15 ◽

2019 ◽

Vol 02 (Bionatura Conference Serie) ◽

Author(s):

Kerly León-Ordóñez ◽

Sofía Abad-Sojos

Keyword(s):

Cervical Cancer ◽

Early Detection ◽

High Sensitivity ◽

Hpv Infection ◽

Molecular Techniques ◽

Cancer Development ◽

Viral Oncoproteins ◽

Hpv E6 ◽

E6 And E7 ◽

Cervical Cancer Development

Human Papillomavirus (HPV) is the predominant cause of cervical cancer worldwide. The infections with HPVs 16 and 18 have a high oncogenic risk for cancer development. Besides, the genes E6 and E7 encode viral oncoproteins associated with infection. New molecular techniques for HPV detection, show important advantages such as high sensitivity, recognition capacity, reliability, among others. These techniques allow the standardization of new protocols associated with the detection in a variety of substances and samples. The stretch relationship between the virus and the disease open a new field to study early detection of the HPV infection. Additionally, less concentration of the sample is needed. Considering the significance of the detection, the present paper explains five novelty molecular applications for the prevention cervical cancer and early detection of HPV such as RNA in situ Hybridization for the detection of HPV E6/E7, genosensors, electrochemical DNA biosensor, PCR-based urine assay and a semen assay for detection of HPV. All the methods related to DNA samples could be used for both genders, there are more acceptable and easy to collect.

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