scholarly journals Nanotebuconazole: Điều chế trong môi trường nước và khảo sát hiệu quả kháng nấm bệnh gây hại trên cây trồng

2021 ◽  
Vol 57 (5) ◽  
pp. 15-20
Author(s):  
Quốc Yên Phạm ◽  
Quốc Tuấn Trần
Keyword(s):  
Polyethylene Glycol ◽  
Minimum Inhibitory Concentration ◽  
Rhizoctonia Solani ◽  
Ralstonia Solanacearum ◽  
Inhibitory Concentration ◽  
Phytophthora Capsici ◽  
Viet Nam ◽  
Mic Minimum Inhibitory Concentration

Tebuconazole được phân tán trong hỗn hợp polyethylene glycol (PEG) và nước theo một tỉ lệ xác định với sự hiện diện của dichloromethane (DCM). Hỗn hợp sau đó được làm bay hơi ở 40oC dưới áp suất 480 mbar để loại bỏ DCM và hình thành nanotebuconazole. Kích thước hạt nanotebuconazole được xác định trong khoảng 27 – 35 nm với kích thước trung bình là 29 nm  bằng kĩ thuật tán xạ ánh sáng động (DLS) và kết quả nghiên cứu cho thấy tỉ lệ PEG:H2O 4:1 là thuận lợi nhất cho quá trình hình thành hạt nhũ nano. Các thử nghiệm in vitro bằng phương pháp MIC (minimum inhibitory concentration) chứng minh rằng dạng nanotebuconazole cho hiệu quả kháng khuẩn, kháng nấm mạnh gấp 2 lần dạng thương mại trên các chủng Ralstonia solanacearum, Fusarium ambrosium và Rhizoctonia solani và gấp 4 lần trên chủng Phytophthora capsici, với nồng độ ức chế tối thiểu lần lượt là 50 ppm và 25 ppm. Chế phẩm nanotebuconazole được đánh giá chất lượng đáp ứng đầy đủ tiêu chuẩn thuốc bảo vệ thực vật theo Tiêu chuẩn Việt Nam 9482:2012 (TCVN 9482:2012).

scholarly journals Potensi Minyak Atsiri Cananga odorata dan Cymbopogon citratus Dalam Menghambat Pertumbuhan Staphylococcus aureus ATCC 25923 dan Candida albicans ATCC 10231 Secara In Vitro

2020 ◽  
Vol 7 (2) ◽  
pp. 48
Author(s):  
Kadek Wegi Kurnilia ◽  
Sang Ketut Sudirga ◽  
Yan Ramona
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Nutrient Agar ◽  
Cymbopogon Citratus ◽  
Mic Minimum Inhibitory Concentration ◽  
Cananga Odorata

Tanaman Cananga odorata dan Cymbopogon citratus merupakan tanaman penghasil minyak atsiri yang memiliki khasiat antimikroba. Tujuan penelitian ini adalah untuk mengetahui potensi minyak atsiri kedua jenis tanaman tersebut dalam menghambat pertumbuhan Staphylococcus aureus dan Candida albicans. Nilai MIC (Minimum Inhibitory Concentration) minyak atsiri C.odorata dan C.citratus dengan perbandingan 1:1 terhadap S.aureus dan C.albicans juga ditentukan dalam penelitian ini. Bioassay dilakukan pada medium Nutrient Agar (NA) atau Potato Dextriose Agar (PDA) yang ditambahkan dengan minyak atsiri kedua tanaman dengan perbandingan konsentrasi yang bervariasi. Medium yang ditambahkan aquades steril berperan sebagai kontrol. Data yang diperoleh pada penelitian ini dianalisis secara kuantitatif dengan bantuan software SPSS For Windows versi 22. Bila diperoleh hasil yang berbeda nyata pada p<0.05, maka analisis dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa semua perlakuan pada penelitian ini mengeliminasi bakteri S.aureus. Sementara itu, C.albicans tidak terhambat pertumbuhannya oleh perlakuan 100% minyak atsiri bunga C.odorata. Eliminasi kamir ini terjadi ketika minyak atsiri bunga kenanga dikombinasi dengan minyak atsiri daun serai dapur. Jika dibandingkan dengan kontrol, semua perlakuan minyak atsiri dengan perbandingan yang bervariasi menunjukkan hasil yang berbeda nyata (p<0.05). Nilai MIC campuran minyak atsiri dengan perbandingan 1:1 pada S.aureus  (MRSA) dan C.albicans  (SC5314) berturut-turut adalah 0,1% dan 2,6%.   Kata-kata kunci: minyak atsiri, Cananga odorata, Cymbopogon citratus, Staphylococcus aureus, Candida albicans

scholarly journals Susceptibility in vitro of clinical Candida albicans isolates to the selected azoles

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Bartlomiej Pawlicki ◽  
Anna Biernasiuk ◽  
Monika Jonczyk ◽  
Anna Malm
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Fungal Infection ◽  
Inhibitory Concentration ◽  
Invasive Disease ◽  
Yeast Strains ◽  
Resistance Rates ◽  
Anti Fungal ◽  
Mic Minimum Inhibitory Concentration

Abstract Candida spp. is the most prevalent cause of fungal infection worldwide, and their increasing resistance to anti-fungal agents, especially to azoles, has become problematic. The aim of this work was to establish the susceptibility to fluconazole, itraconazole, voriconazole and posaconazole of 50 clinical C. albicans isolates from hematooncological patients. This has been evaluated using the following parameters: MIC (Minimum Inhibitory Concentration), MIC50 (MIC required to inhibit the growth of 50% of organisms), as well as MIC90 (MIC required to inhibit the growth of 90% of organisms). Susceptibility of the studied clinical isolates to all azoles was high, being 86% for itraconazole, 90% for fluconazole and posaconazole and 92% for voriconazole. The resistance rates ranged from 8% (voriconazole), to 12% (itraconazole). The emergence of azole-resistant yeast strains creates a necessity to determine and monitor the sensitivity of the isolated Candida spp., including C. albicans, especially in patients predisposed to life-threating fungal invasive disease.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

scholarly journals THE EFFECT OF PROPOLIS EXTRACT AND PROPOLIS CANDIES ON THE GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC 43718

2017 ◽  
Vol 10 (17) ◽  
pp. 26
Author(s):  
Khodijah Khodijah ◽  
Ratna Farida ◽  
Nurtami Soedarsono
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Spectrophotometric Analysis ◽  
Propolis Extract ◽  
Post Exposure ◽  
Colony Forming Units

Objective: This experiment aimed to analyze the effect of propolis extract and propolis containing candies on the growth of Aggregatibacter actinomycetemcomitans using spectrophotometric analysis and colony-forming units (CFU) counts.Methods: After A. actinomycetemcomitans were exposed to propolis extract and candies, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined with spectrophotometry and post-exposure colony counting.Results: The MIC of propolis extract against A. actinomycetemcomitans was determined to be 10%, and the MBC was 20%. A decrease in the total CFU count of A. actinomycetemcomitans was observed after propolis extract and candy exposure.Conclusions: Propolis extract and propolis candies were effective in inhibiting the growth of A. actinomycetemcomitans ATCC 43718 in vitro.

scholarly journals In VitroActivity of Omadacycline againstChlamydia pneumoniae

2018 ◽  
Vol 63 (2) ◽  
pp. e01907-18 ◽  
Author(s):  
Stephan A. Kohlhoff ◽  
Natalia Huerta ◽  
Margaret R. Hammerschlag
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Chlamydia Pneumoniae ◽  
Inhibitory Concentration ◽  
Content Type

ABSTRACTThein vitroactivities of omadacycline, azithromycin, doxycycline, moxifloxacin, and levofloxacin were tested against 15 isolates ofChlamydia pneumoniae. The minimum inhibitory concentration at which 90% of the isolates ofC. pneumoniaewere inhibited by omadacycline was 0.25 μg/ml (range, 0.03 to 0.5 μg/ml).

Activity of myxin against Ceratocystis ulmi

1969 ◽  
Vol 15 (1) ◽  
pp. 133-135 ◽  
Author(s):  
E. A. Peterson
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Liquid Culture ◽  
Inhibitory Concentration ◽  
In Vitro Tests ◽  
Strong Inhibition ◽  
Solid Media ◽  
Highly Sensitive ◽  
Paper Disc ◽  
Disc Assay

Eight strains of Ceratocystis ulmi originating from different locations and host species were found to be highly sensitive to the antibiotic myxin in in vitro tests. By paper disc assay, amounts as low as 0.5–1.0 μg caused strong inhibition of the fungus on solid media. The minimum inhibitory concentration in liquid culture was 0.2 μg/ml and levels of antibiotic above this concentration proved to be fungicidal.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

scholarly journals In vitro activities of Traganum nudatum and Mentha pulegium extracts combined with amphotericin B against Candida albicans in production of hydrolytic enzymes

2020 ◽  
Author(s):  
Ikram Tefiani ◽  
Sidi Mohammed Lahbib Seddiki ◽  
Moustafa Yassine Mahdad
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Amphotericin B ◽  
Inhibitory Concentration ◽  
Culture Media ◽  
Hydrolytic Enzymes ◽  
Normal Flora ◽  
Mentha Pulegium ◽  
Hydrolytic Activities

Background and Purpose: Candida albicans is an important microorganism in the normal flora of a healthy subject; however, it has an expedient pathogenic character that induces hydrolytic virulence. Regarding this, the present study aimed to find an in vitro alternative that could reduce the virulence of this yeast. Materials and Methods: For the purpose of the study, the effect of amphotericin B (AmB) combined with the extract of Traganum nudatum (E1) or Mentha pulegium (E2) was evaluated against the hydrolytic activities of esterase, protease, and phospholipase. This effect was determined by calculating the minimum inhibitory concentration (MIC), used to adjust the extract/AmB mixtures in culture media. Results: The evaluated Pz values, which corresponded to the different enzymatic activities, showed a decrease in the hydrolytic activities of C. albicans strains after the addition of E1/AmB and E2/AmB combinations at descending concentrations (lower than the obtained MICs). Conclusion: Based on the findings, it would be possible to reduce the pathogenesis of this species without destabilizing the balance of the flora.

Comparison of the in Vitro Activity of Daptomycin and four other Antimicrobial Agents against Staphylococci Associated with CAPD Peritonitis

1988 ◽  
Vol 8 (4) ◽  
pp. 277-279
Author(s):  
Wendy L. Vaudry ◽  
Claudia Gratton ◽  
Kinga Kowalewska ◽  
Wanda M. Wenman
Keyword(s):  
Peritoneal Dialysis ◽  
Minimum Inhibitory Concentration ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Coagulase Negative Staphylococci ◽  
Vancomycin Resistant

The minimum inhibitory concentration (MIC) of daptomycin was compared with that of four other antimicrobial agents against clinically relevant staphylococci. Sixtyfive isolates were obtained from patients on continuous ambulatory peritoneal dialysis (CAPD) who contracted peritonitis. These isolates comprised 29 S. Sureus strains (all sensitive to oxacillin); 25 S. epidermidis strains (14 sensitive and 9 resistant to oxacillin); and 11 unspeciated coagulase-negative staphylococci (2 sensitive and 11 resistant to oxacillin). All of the oxacillin susceptible strains were inhibited by ≤2 mg/L of the five antibiotics tested. The oxacillin resistant staphylococci were also resistant to cefuroxime and variably resistant to cefamandole, but were uniformly susceptible to both vancomycin and daptomycin. Daptomycin possesses equivalent in vitro activity to vancomycin against strains of S. Sureus and coagulase negative staphylococci associated with CAPD peritonitis. If vancomycin resistance becomes a significant problem in these patients, and daptomycin is shown to be active against vancomycin resistant organisms, then it would have potential usefulness as an alternative to vancomycin in the treatment of peritonitis caused by multiply -resistant staphylococci.

scholarly journals Antifungal Effects of Zataria multiflora Essential Oil on the Inhibitory Growth of some Postharvest Pathogenic Fungi

2015 ◽  
Vol 7 (4) ◽  
pp. 412-416
Author(s):  
Mahboobeh NASSERI ◽  
Hossein AROUIEE ◽  
Shiva GOLMOHAMMADZADEH ◽  
Mahmoud Reza JAAFARI ◽  
Hossein NEAMATI
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Alternaria Solani ◽  
Pathogenic Fungi ◽  
Aspergillus Ochraceus ◽  
Malt Extract ◽  
Zataria Multiflora ◽  
Minimum Fungicidal Concentration

The present study aimed to determine minimum inhibitory concentration and minimum fungicidal concentration of the essential oil of Zataria multiflora to control Alternaria solani, Rhizoctonia solani, Rhizopus stolonifer, Aspergillus flavus, Aspergillus ochraceus and Aspergillus niger. The essential oil of Zataria multiflora was tested in vitro on PDA (malt extract agar medium) with eight concentrations: 0, 10, 50, 100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 ppm. This investigation followed the completely randomized design (CRD) with three replications. GC-MS evaluations of the essential oil revealed that thymol (35%), carvacrol (34%), cymene-p (9.89%), gamma-terpinene (5.88%) and alpha-pinene (4.22%) were the main compounds of Zataria multiflora oil. The results showed that the essential oil of Zataria multiflora has antifungal activity; the lowest inhibition (75%) was observed in the A. niger, while the highest inhibition (95.3%) was observed in A. solani. Minimum inhibitory concentration for A. solani, R. solani, R. stolonifer, A. flavus, A. ochraceus and A. niger was 200, 200, 200, 300, 300 and 200 ppm respectively. In addition, the present results showed that minimum fungicidal concentration (MFC) for A. solani, R. solani, R .stolonifer, A. niger and A.ochraceus was 600, 400, 300, 900 and 700 ppm respectively and none of the tested concentrations were fatal for A. flavus. A. solani and R. solani showed a strong sensitivity to Zataria multiflora essential oil at all concentrations. Findings of the current study suggest that essential oils of Zataria multiflora could be used for control of postharvest phytopathogenic fungi on fruits or vegetables.

Sign in / Sign up

Export Citation Format

Share Document