scholarly journals OLIGO-GLUCOMANNAN PRODUCTION FROM PORANG (Amorphophallus oncophyllus) GLUCOMANNAN BY ENZYMATIC HYDROLYSIS USING β-MANNANASE

2021 ◽  
Vol 17 (1) ◽  
pp. 23
Author(s):  
Anggela Anggela ◽  
Widiastuti Setyaningsih ◽  
Santad Wichienchot ◽  
Eni Harmayani
Keyword(s):  
Reaction Time ◽  
Enzymatic Hydrolysis ◽  
Optimum Condition ◽  
Hplc Analysis ◽  
Degree Of Polymerization ◽  
Reducing Sugar ◽  
Optimal Conditions ◽  
Hydrolysis Process ◽  
Alternative Approach ◽  
Potential Substrate

Porang (Amorphophallus oncophyllus) is an indigenous tuber of Indonesia that rich in glucomannan. An alternative approach to produce porang oligo-glucomannan (POG) as prebiotic from porang glucomannan (PGM) was made by enzymatic hydrolysis using β-mannanase. This study aimed to  produce POG under optimal conditions by controlled enzymatic hydrolysis process. The PGM flour contained 96.12% of indigestible carbohydrates. The optimum condition of enzymatic hydrolysis producing the highest reducing sugar was as follows: temperature 37°C, pH 5.5, a ratio of enzyme to the substrate (E/S) 1:1000, and reaction time 4 h. HPLC analysis confirmed that 99.45% of the resulting POG consisted of oligosaccharides with a degree of polymerization (DP) 3. Hence, the PGM utilized in this study has been proven as a potential substrate for POG production. Additionally, the resulting POG was considered as a functional ingredient due to has prebiotic potential.

scholarly journals Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Vera BARLIANTI ◽  
Deliana DAHNUM ◽  
. MURYANTO ◽  
Eka TRIWAHYUNI ◽  
Yosi ARISTIAWAN ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Volume Ratio ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Economic Feasibility ◽  
First Order ◽  
Empty Fruit Bunches ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    

scholarly journals Enzymatic hydrolysis of bitter cassava and Gadung starches with different compositions at low temperature

2018 ◽  
Vol 156 ◽  
pp. 01009
Author(s):  
Hargono Hargono ◽  
Andri Cahyo Kumoro ◽  
Bakti Jos
Keyword(s):  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Optimum Condition ◽  
Starch Content ◽  
Reducing Sugar ◽  
Hydrolysis Time ◽  
Bitter Cassava ◽  
Manihot Glaziovii ◽  
Hydrolysis Of ◽  
Granular Starch

The effect of compositions of bitter cassava (Manihot glaziovii) and gadung (Dioscorea hispida Dennst) starches on reducing sugar during hydrolysis using granular starch hydrolyzing enzyme (GSHE) was studied. All hydrolyses were conducted at concentration of substrate was 200 g.L-1, while concentration of enzyme was 1.5 % (w/w), during of hydrolysis time 24 h, at 30°C. Mass compositition of bitter cassava and gadung starches were 9:1 to 1:9 The increase gadung starch compositions will decrease the reducing sugar. The optimum condition of the process using concentration of substrate 200 g.L-1 with compositions of bitter cassava and gadung starches was 9:1 at 18 h. It was found that reducing sugar was 50.20 g.L-1. The concentration of reducing sugar mainly depend on starch content on bitter cassava, it is much bigger than the gadung starch.

scholarly journals Enzymatic Hydrolysis of Liquid Hot Water Pre-treated Macro-alga (Ulva lactuca) for Fermentable Sugar Production

2018 ◽  
Vol 156 ◽  
pp. 01015
Author(s):  
Tri Poespowati ◽  
Ardy Riyanto ◽  
Hazlan ◽  
Ali Mahmudi ◽  
Rini Kartika-Dewi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Reducing Sugar ◽  
Ulva Lactuca ◽  
Hot Water ◽  
Cellulose Content ◽  
Hydrolysis Time ◽  
Liquid Hot Water ◽  
Hydrolysis Process ◽  
Pre Treatment ◽  
Ph Level

Ulva lactuca is one of green macro-algae that has a significant cellulose content. This study aims to determine the effect of variations in substrate-enzyme ratio and hydrolysis time on the enzymatic hydrolysis process of cellulose extracted from Ulva lactuca to produce fermentable sugar or reducing sugar as a raw material for making bioethanol. Firstly, Liquid Hot Water (LHW) pre-treatment process was performed at the temperature of 135°C for 20 minutes; the purpose of this pre-treatment was to reduce the content of hemicellulose and to increase the cellulose content. Secondly, enzymatic hydrolysis process using cellulase enzyme was carried out, in this process citrate buffer was needed in order to stabilize the pH level during hydrolysis process. The process variables were ratio of substrate-enzyme (1:0.05; 1:0.1; 1:1.5; 1:2 and 1:2.5 w/w) and hydrolysis time (24, 48 and 72 hours) under temperature of 45°C and pH level of 5.5. The results shows that the highest reducing sugar yield is 79.7% obtained at a ratio of substrate-enzyme of 1:2.5 (w/w) for 48 hours of hydrolysis time, with the result of reducing sugar concentration is 16.2043 mg/mL.

scholarly journals Effect of Ultrasound-Assisted on Sequential Peracetic Acid and Alkaline Peroxide Pretreatment to The Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch

2021 ◽  
Author(s):  
Dwini Normayulisa Putri ◽  
Meka Saima Perdani ◽  
Masafumi Yohda ◽  
Tania Surya Utami ◽  
Muhamad Sahlan ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Peracetic Acid ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Empty Fruit Bunch ◽  
Hydrolysis Time ◽  
Alkaline Peroxide ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstract Enzymatic hydrolysis of oil palm empty fruit bunch (OPEFB) that has been pretreated by modified pretreatment has been investigated in this study. The OPEFB used was pretreated by using sequential peracetic acid – alkaline peroxide solution. As the modification method, the assistance of pretreatment by ultrasound was conducted, in order to increase the enzyme accessibility. Therefore, it enhances the production of reducing sugar on the hydrolysis process. Prior to hydrolysis process, OPEFB was initially treated by using peracetic acid solution, comprise of CH3COOH (> 99%) and H2O2 (30% w/w), assisted by ultrasound for 3 hours at 35oC. Afterwards, OPEFB was treated by using alkaline peroxide solution, comprise of NaOH (40% w/w) and H2O2 (35% w/w), assisted by ultrasound for 10 hours at 35oC. OPEFB that has been pretreated was then subjected to enzymatic hydrolysis process using cellulase enzyme, in order to convert cellulose content into reducing sugar. Enzymatic hydrolysis was carried out at 50oC in a shaker incubator with 150 rpm for 48 hours. In this study, the effect of different enzyme concentration and hydrolysis time towards the sugar concentration in modified-pretreated OPEFB was observed and analyzed. Three different concentrations of enzyme were used, including 1.25, 2.5, and 5 g/L, and reducing sugar concentrations were analyzed at 30 and 45 minutes, and 1, 2, 4, 6, 24, 30, and 48 hours. Based on results, enzyme concentration has a significant effect to the production of reducing sugar. The reducing sugar concentrations obtained at the end of the hydrolysis process were 8.48, 11.06, 19.16 g/L, at the enzyme concentrations of 1.25, 2.5, and 5 g/L, respectively. At any hydrolysis time, the highest sugar concentration has been achieved on the highest enzyme concentration of 5 g/L. Moreover, the effective hydrolysis time were achieved at 6 hours, at all concentration of enzyme, since the production of reducing sugar were insignificant after 6 hours. This study showed an increase in reducing sugar production by 8.25% in the hydrolysis process using OPEFB pretreated by modified pretreatment compared to the non-modified pretreatment.

scholarly journals BIOSINTESIS NANOPARTIKEL PERAK MENGGUNAKAN EKSTRAK BUAH Passiflora flavicarva (MARKISA) UNTUK MENDETEKSI LOGAM BERAT

Alotrop ◽  
2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Dina Maryani ◽  
M. Lutfi Firdaus ◽  
Nurhamidah Nurhamidah
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Silver Nanoparticles ◽  
Reaction Time ◽  
Optimum Condition ◽  
Passion Fruit ◽  
Optimal Conditions ◽  
Optimum Conditions ◽  
Fruit Extract ◽  
Minimum Concentration

The aim of this research was to synthesize silver nanoparticle (NPP) by using Passiflora flavicarva (Markisa) fruit extract as detector of heavy metal. The NPP particles produced were determined by their optimum condition on the ratio of the volume of  Passiflora flavicarva passion fruit extract to AgNO3 and the reaction time. The silver nanoparticles produced under optimum conditions will be tested for selectivity against several metals that are often present in the environment, once the selective metal is known then it can be sensitized to the metal using a UV-Vis spectrophotometer to determine the minimal concentrations that can be detected by the silver nanoparticles Formed. The results showed that Passiflora flavicarva passion fruit extracts could be used for the biosynthesis of  NPP particles because they contain vitamin C and flavonoids capable of reducing Ag+ to Ag0 in the form of NPP. The optimal conditions for forming NPP particles are in the 1: 2 ratio with a 2 hour reaction time. From the results of selectivity test of NPP to some heavy metals that often exist in environment, it is known that NPP selective to mercury metal. It was found that selective mercury was followed by a sensitivity test with a UV-Vis spectrophotometer, with sensitive NPP  up to a concentration of 6.7 ppm. From the results of this study it can be concluded that NPP particles formed can be used to detect mercury metal up to a minimum concentration of 6.7 ppm.

scholarly journals Conversion of Lignocellulosic Material Into Fermentable Sugars

2017 ◽  
Vol 12 (4) ◽  
pp. 141-153
Author(s):  
Asem Hassan Mohammed ◽  
Frank Behrendt Behrendt ◽  
Frank Jürgen Methner
Keyword(s):  
Enzymatic Hydrolysis ◽  
Structural Features ◽  
Impact Factors ◽  
Optimal Conditions ◽  
Short Term ◽  
Water Loading ◽  
Lime Pretreatment ◽  
Hydrolysis Process ◽  
Biomass Materials ◽  
Optimal Values

Enzymatic hydrolysis process of lignocellulosic biomass materials is difficult because of inherent structural features of biomass, which represents barriers that prevent complete hydrolysis; therefore, pretreatment techniques are necessary to render biomass highly digestible in enzymatic hydrolysis process. In this research, (non?) oxidative short-term lime pretreatment of willow wood was used. A weight of  11.40 g of willow wood was mixed with an excess of calcium hydroxide (0.4 g Ca(OH)2/g raw biomass) and water loading (15 g/g raw biomass). Lime pretreatment was carried out for various periods of time including 1, 2, 3.5, 5 and 6 h, with temperatures at 100, 113, 130, 147 and 1600C, and oxygen pressures as oxidativeagent (6, 9, 13.5, 17.8, 21 bar absolute). The optimization of both pretreatment and enzymatic hydrolysis were depended on the maximum overall yields of glucan and xylan after two processes of lime pretreatment and enzymatic hydrolysis. The optimal conditions of pretreatment were as follow: 1) 1.33 h, 1470C, 17.8 bar absolute, 0.26 g Ca(OH)2/g raw biomass. 2) 1.25 h, 155 0C, 21 bar absolute, 0.26 Ca(OH)2/g raw biomass. Furthermore, the optimal values for low impact factors such as water loading was 15 g/g raw biomass and particle size was less than 3 mm. The optimal conditions of enzymatic hydrolysis were as follow: Cellulase enzymeloading was 0.1 g /g glucan in raw biomass, at substrate concentration of 50 g/L during 72 h of enzymatic hydrolysis The yield of enzymatic hydrolysis under these conditions were as follow: 96.00 g glucan/100 g of glucan in raw biomass, and 65.00 g xylan/100 g xylan in raw biomass.

Bioethanol Production from Sweet Potato Using Combination of Acid and Enzymatic Hydrolysis

2011 ◽  
Vol 110-116 ◽  
pp. 1767-1772 ◽  
Author(s):  
Eka Putri Lily Surayya ◽  
Nasrulloh ◽  
Haris Abdul
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Potato Starch ◽  
Starch Content ◽  
Sugar Content ◽  
Reducing Sugar ◽  
Sweet Potatoes ◽  
Fermentation Time ◽  
Hydrolysis Process

Indonesia is rich in natural resources, including source of biofuel such as sweet potato starch. Sweet potato (Ipomoea batatasL) has a starch content of 22.4% which could be converted to sugar through hydrolysis process in order to yield ehtanol. This study aimed to examine the optimize ethanol yielded from starch of sweet potatoes using combination of acid and enzymatic hydrolysis. It used 0.5 HCl which combined with eachAspergillus flavusandAspergillusniger,and mixture of both microbes for acid and enzymatic hydrolysis process. The highest sugar content resulted in hydrolysis process was fermented for 24, 48, and 72 hours to yield maximum ethanol. Data were analyzed using one-way analysis of variance (Anova). The highest reducing sugar produced by combination of HCl andAspergillus nigerwas 12.61% (w/v). The highest ethanol produced was 46.17% (v/v) in 72 hours fermentation time.

Enzymatic Preparation of Nanocrystalline Cellulose from Bamboo Fibers

2012 ◽  
Vol 441 ◽  
pp. 754-758 ◽  
Author(s):  
Yong Zhang ◽  
Guo Xin Xue ◽  
Xiu Mei Zhang ◽  
Yu Zhao
Keyword(s):  
Enzymatic Hydrolysis ◽  
Average Diameter ◽  
Degree Of Polymerization ◽  
Nanocrystalline Cellulose ◽  
Preparation Process ◽  
Enzymatic Preparation ◽  
Bamboo Pulp ◽  
Transmission Electron ◽  
Hydrolysis Process ◽  
Bamboo Fibers

This paper reports an attempt to prepare nanocrystalline cellulose from bamboo fibers through an enzymatic hydrolysis process. A type of commercial cellulase, viz. enzyme Cclast (mainly containing endoglucanase) was used in the preparation process. The morphology of the prepared bamboo cellulose nanocrystals was characterized by transmission electron microscopy (TEM) and the sugar analyses of hydrolysis residues were analyzed by ion chromatography (IC). The degree of polymerization (DP) was tested by automatic viscosimeter. All nanocrystalline cellulose from bamboo fibers presented a rod-like shape, an average diameter (D) of 5.3 nm and length (L) of 221 nm, with an aspect ratio (L/D) of around 42. It was shown that the use of enzymatic hydrolysis treatment to bleach bamboo pulp helps the preparation of well individualized rod-like nanocrystalline cellulose.

scholarly journals IMPROVEMENT OF BIOETHANOL PRODUCTION FROM LOW GRADE AND DAMAGED LONGAN FRUITS WITH THERMAL PRETREATMENT AND DIFFERENT TYPES OF THE ENZYMATIC HYDROLYSIS

2020 ◽  
pp. 6-11
Author(s):  
TU VY THUY NGUYEN ◽  
YUWALEE UNPAPROM ◽  
PIYAPAT CHAICHOMPOO ◽  
RAMESHPRABU RAMARAJ
Keyword(s):  
Enzymatic Hydrolysis ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Low Grade ◽  
Sugar Production ◽  
Bioethanol Production ◽  
Thermal Pretreatment ◽  
Hydrolysis Process ◽  
Different Types ◽  
Hydrolysis Of

Pretreatment is a vital step in the enzymatic hydrolysis of biomass and the successive production of bioethanol. The present study is focused on thermal pretreatment (boiling & autoclave) methods of low grade and damaged longan fruits using three different types of the enzymatic sources from commercial cellulase, an enzyme from algae and mixed enzymes (i.e., commercial cellulase with algal enzyme). Total sugar production after the hydrolysis process from commercial cellulase, the enzyme from algae and mixed enzymes were 326.41 ± 08.97 g/L, 348.68 ± 01.95 g/L and 368.42 ± 01.16 g/L, respectively. Reducing sugar after the hydrolysis process generated from commercial cellulase, the enzyme from algae and mixed enzymes was 182.54 ± 03.05 g/L, 183.33 ± 04.70 g/L and 297.78 ± 02.94 g/L, respectively. Fermentation of these hydrolysate using Saccharomyces cerevisiae TISTR 5020 produced the highest ethanol production from using commercial cellulase, the enzyme from algae and mixed enzymes was 16.74 ± 0.62 g/L, 5.38 ± 0.54 g/L and 14.32 ± 1.89 g/L, respectively. Consequently, this study suggested that suitable pretreatment and hydrolysis processes are performing a significant role in bioethanol production from low grade and damaged longan fruits.

scholarly journals Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Vera BARLIANTI ◽  
Deliana DAHNUM ◽  
. MURYANTO ◽  
Eka TRIWAHYUNI ◽  
Yosi ARISTIAWAN ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Volume Ratio ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Economic Feasibility ◽  
First Order ◽  
Empty Fruit Bunches ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    

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