scholarly journals STUDY ON CHEMICAL CONSTITUENT AND BIOACTIVITIES OF THE FRUITS OF DIPTEROCARPUS RETUSUS BLUME. DIPTEROCARPACEAE OF VIETNAM

2019 ◽  
Vol 57 (3) ◽  
pp. 294
Author(s):  
Ho Dac Hung ◽  
Doan Huy Tien ◽  
Nguyen Thi Ngoan ◽  
Ba Thi Duong ◽  
Do Quoc Viet ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Chemical Constituent ◽  
Cytotoxic Activities ◽  
Spectroscopic Methods ◽  
Trans Stilbene

The first study on chemical constituents and biological activities of fruits of Dipterocarpus retusus Blume (Dipterocarpaceae.) growing in Vietnam was reported. Column chromatography of the ethyl acetate extract led to the isolation of five compounds: eleutherol (1), 3, 5, 4' - trihydroxy - trans - stilbene (2), polydatin (3) and β-sitosterol (4), β-sitosterol-3-O-β-D-glucopyranoside (5). Their structures were elucidated based on the NMR spectroscopic methods and comparison with literature date. The extracts from fruits of D. retusus Blume by n-hexane, ethyl acetate and butanol, consecutively, were evaluated for antioxidative and cytotoxic activities.

In Vitro Screening for Cytotoxic, Anti-bacterial, Anti-HIV1-RT Activities and Chemical Constituents of Croton fluviatilis, Croton acutifolius, and Croton thorelii

2021 ◽  
Vol 11 ◽  
Author(s):  
Charina Worarat ◽  
Wilart Pompimon ◽  
Phansuang Udomputtimekakul ◽  
Sukee Sukdee ◽  
Punchavee Sombutsiri ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Bacterial Activity ◽  
Cytotoxic Activities ◽  
Separation And Purification ◽  
Crude Extracts ◽  
Cytotoxicity Activities

Background: Although the chemical constituents and biological activities of a large number of plants in the Croton genus have been studied, there are still recently discovered plants to be investigated. Objective: 1. To investigate the anti-bacterial, anti-HIV1-RT, and cytotoxicity activities of crude extracts from these plants. 2. To investigate the chemical constituents of Croton fluviatilis, Croton acutifolius, and Croton thorelii. Method: The anti-bacterial, anti-HIV1-RT, and cytotoxicity of the three plants were evaluated by standard techniques. Extraction, separation, and purification of extracts from the three plants were undertaken. Results: The ethyl acetate extract of C. fluviatilis showed low anti-bacterial activity against E. aerogenes, E. coli 0157: H7, and P. mirabilis, together with the ethyl acetate extract of C. acutifolius displayed low anti-bacterial activity against E. aerogenes, while all the crude extracts of C. thorelii were inactive. The ethyl acetate extracts of C. thorelii, and C. fluviatilis showed strong inhibited HIV1-RT, whereas the ethyl acetate extract of C. acutifolius, and the hexane extract of C. fluviatilis displayed moderate inhibited HIV1-RT. Cytotoxic properties of three Croton plants were specific to KKU-M213, MDA-MB-231, A-549, and MMNK-1. Especially, the ethyl acetate extract of C. acutifolius exhibited strong cytotoxic activities against MDA-MB-231, A-549, and MMNK-1. Furthermore, the ethyl acetate extract of C. thorelii showed high cytotoxic activities against KKU-M213, and MDA-MB-231. Compounds 1, and 4 were found in C. fluviatilis. Compounds 2 and 4 were also found in C. acutifolius. Moreover, compound 3 was only found in C. thorelii. Conclusion: The present study revealed that the three Croton species are good sources of flavonoid compounds and further investigation of the chemical constituents from these plants may prove to be fruitful to discover more active compounds to be tested as potential medicines.

scholarly journals CONTRIBUTE TO STUDY ON CHEMICAL CONSTITUENTS OF URENA LOBATA (L.) OF VIETNAM

2019 ◽  
Vol 57 (2) ◽  
pp. 162
Author(s):  
Quan Minh Pham ◽  
Hoai Van Thi Tran ◽  
Lam Tien Do ◽  
Phuong Lan Doan ◽  
Inh Thi Cam ◽  
...  
Keyword(s):  
Mental Disorders ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Tree Roots ◽  
Chemical Structures ◽  
Phytochemical Investigation ◽  
First Time

Urena lobata L. is used in Vietnamese traditional medicine for the treatment of several diseases. Tree roots are used to treat rheumatism, dysentery, poor digestion, flu, tonsils, malaria, asthma, goiter. Flowers are used to treat chickenpox, fever, and mental disorders. Branches, leaves or whole trees used to treat injuries bruises, rheumatism, mastitis, bites. Phytochemical investigation of the n-hexan and ethyl acetate extract of Urena lobata L. led to the isolation of β-sitosterol (1), β-sitosterol-3-O-β-D-glucopyranoside (2), a-acetylamino-phenylpropyl a-benzoylamino-phenylpropanoate (3), quercetin (4), and trans-tiliroside (5). Their chemical structures were determined by spectroscopic methods including MS, 1D, 2D NMR and comparing with those reported in previous papers. Two compounds 3, 5 were isolated for the first time from Urena lobata plant.

scholarly journals Studies on Chemical Constituents and Biological Activities of an Endophytic Fungi from Magnifera indica L.

2015 ◽  
Vol 13 (1) ◽  
pp. 63-67
Author(s):  
Mozammel Haque ◽  
Mohammad Shoeb ◽  
Nilufar Nahar
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Acid Methyl Ester ◽  
Diffusion Method ◽  
Published Data ◽  
Nmr Studies ◽  
Brine Shrimp Lethality Assay

Two compounds, ergosterol (1) and 4-hydroxy-hexadec-6-enoic acid methyl ester (2) were isolated from the ethyl acetate extract of the endophytic fungal strain labeled as MI-3, isolated from the leave of Magnifera indica L. The structures of the isolated compounds were elucidated by 1H NMR studies and comparing with published data. The crude ethyl acetate extract, three column fractions and ergosterol were tested for antimicrobial activity against five Gram-positive and eight Gram-negative bacteria and three fungi by disc diffusion method. The general toxicity and antioxidant activity of the parent extract, column fractions and ergosterol were also evaluated by using brine shrimp lethality assay and free radical scavenging assay, respectively. Low activities were observed in all cases. DOI: http://dx.doi.org/10.3329/dujps.v13i1.21862 Dhaka Univ. J. Pharm. Sci. 13(1): 63-67, 2014 (June)

scholarly journals ANTIOXIDANT ACTIVITY OF 2,6,4’-TRIHYDROXY-4-METHOXY BENZOPHENONE FROM ETHYL ACETATE EXTRACT OF LEAVES OF MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.)

2011 ◽  
Vol 11 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Susilawati Susilawati ◽  
Sabirin Matsjeh ◽  
Harno Dwi Pranowo ◽  
Chairil Anwar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Column Chromatography ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Chemical Constituent ◽  
Traditional Medicines ◽  
Phaleria Macrocarpa ◽  
Spherical Crystal

Mahkota dewa plant (Phaleria macrocarpa (Scheff.) Boerl.) which is included into family of Thymelaeaceae is one of Indonesia's traditional medicines. Chemical constituent has been isolated from ethyl acetate extract of leaves of mahkota dewa. Sample was extracted with methanol, concentrated then extracted by n-hexane, chloroform and ethyl acetate. The ethyl acetate extract was separated and fractionated by column chromatography. The first fraction was purified by TLC preparative and recrystalization. Compound was isolated as red-brown spherical crystal in 8 mg (m.p. 129-131 °C). Its spot gave dark fluoroscence at TLC plate (UV366) with Rf of 0.3 at TLC chromatogram with eluent of n-hexane : ethyl acetate (7:3); 0.6 with n-hexane : ethyl acetate (1:1); 0.9 with -hexane : ethyl acetate (4:6). This compound was dissolved in methanol. Compound was identified by UV, IR, 1H NMR, 13C NMR and NMR 2 dimension (HMQC, COSY, HMBC and DEPT-135) spectroscopic as 2,6,4'-trihydroxy-4-methoxybenzophenon. This compound as well as the ethyl acetate extract showed antioxidant activity on DPPH with IC50 was 10.57 and 101.06 μg/mL, respectively. This compound showed strong antioxidant activity on DPPH, almost to the standard antioxidant activity of quercetin (IC50 of 2.93 μg/mL)

scholarly journals Investigation of bioative compounds from the ethyl acetate extract of Euphorbia hirta Linn

2018 ◽  
Vol 1 (T5) ◽  
pp. 95-101
Author(s):  
Ngan Thi Kim Tran ◽  
Ly Thi Le ◽  
Nhi Thi Y Nguyen ◽  
Minh Thi Tran ◽  
Quan Le Tran
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Gastrointestinal Disorders ◽  
Published Data ◽  
Spectroscopic Methods ◽  
Asian Countries ◽  
Euphorbia Hirta ◽  
Chemical Structures ◽  
The Past

Euphorbia hirta Linn. (co sua la lon in Vietnamese) belongs to Euphorbiaceae family, is a group of small prostrate herbaceous annual weed in Vietnam. It is abundant in waste places and open grasslands and distributes in most Asian countries. E. hirta is traditionally used in the treatment of gastrointestinal disorders, bronchial and respiratory. The aqueous extract exhibits anxiolytic, analgesic, antipyretic, and anti-inflammatory activities. Strong anti-diabetic activity of Euphorbiaceae family in general and E. hirta in particular was reported in the past investigations. E. hirta has been studied by various investigations and several active constituents have been isolated and identified successfully. Most of those compounds have strong biological activities. At the first step in the processing of the isolation of bioactive compounds from the ethyl acetate extract, we isolated four purified compounds, including methyl gallate (1), quercetin (2), myrecitin (3), and quercitrin (4). The chemical structures of those compounds were elucidated by spectroscopic methods and compared with published data in the literature.

scholarly journals Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4009
Author(s):  
Ahmed S. Abu Zaid ◽  
Ahmed E. Aleissawy ◽  
Ibrahim S. Yahia ◽  
Mahmoud A. Yassien ◽  
Nadia A. Hassouna ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Streptomyces Griseus ◽  
Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Chemical Structures ◽  
Bioassay Guided Fractionation

Background: This study aimed to produce, purify, structurally elucidate, and explore the biological activities of metabolites produced by Streptomyces (S.) griseus isolate KJ623766, a recovered soil bacterium previously screened in our lab that showed promising cytotoxic activities against various cancer cell lines. Methods: Production of cytotoxic metabolites from S. griseus isolate KJ623766 was carried out in a 14L laboratory fermenter under specified optimum conditions. Using a 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium-bromide assay, the cytotoxic activity of the ethyl acetate extract against Caco2 and Hela cancer cell lines was determined. Bioassay-guided fractionation of the ethyl acetate extract using different chromatographic techniques was used for cytotoxic metabolite purification. Chemical structures of the purified metabolites were identified using mass, 1D, and 2D NMR spectroscopic analysis. Results: Bioassay-guided fractionation of the ethyl acetate extract led to the purification of two cytotoxic metabolites, R1 and R2, of reproducible amounts of 5 and 1.5 mg/L, respectively. The structures of R1 and R2 metabolites were identified as β- and γ-rhodomycinone with CD50 of 6.3, 9.45, 64.8 and 9.11, 9.35, 67.3 µg/mL against Caco2, Hela and Vero cell lines, respectively. Values were comparable to those of the positive control doxorubicin. Conclusions: This is the first report about the production of β- and γ-rhodomycinone, two important scaffolds for synthesis of anticancer drugs, from S. griseus.

CHEMICAL CONSTITUENTS OF ETHYL ACETATE EXTRACT FROM Taxus wallichiana ZUCC. (TAXACEAE) LEAVES COLLECTED IN HA GIANG PROVINCE

2020 ◽  
Vol 65 (6) ◽  
pp. 110-115
Author(s):  
Dien Pham Huu ◽  
My Pham Thi ◽  
Nam Bui Tuan
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Spectroscopic Methods ◽  
First Time ◽  
Taxus Wallichiana

Investigation on the chemical constituents of ethyl acetate extract from leaves of Taxus wallichiana Zucc. (Taxaceae), collected in Ha Giang province, has resulted in the isolation of three metabolites for the first time: sciadopitysin (1), β-sitosterol (2), and isoquercitrin (3). Their structures were determined by spectroscopic methods.

Study on Chemical Constituents and Cytotoxic Activities of Salacia chinensis Growing in Vietnam

2010 ◽  
Vol 65 (10) ◽  
pp. 1284-1288 ◽  
Author(s):  
Tran Thi Minh ◽  
Nguyen Thi Hoang Anh ◽  
Vu Dao Thang ◽  
Tran Van Sung
Keyword(s):  
Spectral Analysis ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Cytotoxic Activities ◽  
Good Activity ◽  
Hep G2 ◽  
Mcf 7

Two new triterpenoids, named 7α,21α-dihydroxyfriedelane-3-one (1) and 7α,29-dihydroxyfriedelane- 3-one (2) have been isolated from the ethyl acetate extract of the stems of Salacia chinensis besides the known triterpenoid 21α,30-dihydroxyfriedelane-3-one (3). The structures of the isolated compounds were elucidated on the basis of spectral analysis. Eight triterpenoids from this plant have been tested against the four cancer cell lines Hep-G2, LU, KB, and MCF-7. The new compound 1 showed good activity against all four tested cell lines.

scholarly journals Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort

2021 ◽  
Vol 12 ◽  
Author(s):  
Zizhong Tang ◽  
Yihan Qin ◽  
Wenhui Chen ◽  
Zhiqiao Zhao ◽  
Wenjie Lin ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Penicillium Oxalicum ◽  
Ligusticum Chuanxiong ◽  
Butanol Extract

The objective of this study was to evaluate the diversity of endophytic fungi of different parts of Ligusticum chuanxiong Hort (CX) and further characterize their biological activities and identify chemical compounds produced by these endophytic fungi. A total of 21 endophytic fungi were isolated and identified from CX. Penicillium oxalicum, Simplicillium sp., and Colletotrichum sp. were identified as promising strains by the color reaction. Comparing different organic extracts of the three strains, it was observed that the ethyl acetate extract of Penicillium oxalicum and Simplicillium sp. and the n-butanol extract of Colletotrichum sp. showed significant antioxidant and antibacterial activities. The ethyl acetate extracts of Penicillium oxalicum had outstanding antioxidant and antibacterial effects, and its radical scavenging rates for ABTS and DPPH were 98.43 ± 0.006% and 90.11 ± 0.032%, respectively. At the same time, their IC50 values were only 0.18 ± 0.02 mg/mL and 0.04 ± 0.003 mg/mL. The ethyl acetate extract of Penicillium oxalicum showed MIC value of only 0.5 mg/mL against Escherichia coli and Staphylococcus aureus. By liquid chromatography-mass spectrometry (LC-MS), we found that Penicillium oxalicum could produce many high-value polyphenols, such as hesperidin (36.06 μmol/g), ferulic acid (1.17 μmol/g), and alternariol (12.64 μmol/g), which can be a potential resource for the pharmaceutical industry. In conclusion, these results increase the diversity of CX endophytic fungi and the antioxidant and antibacterial activities of their secondary metabolites.

scholarly journals New Insights into cytotoxic metabolites produced by Streptomyces griseus isolate KJ623766: Large scale production, structure elucidation and biological activities

2019 ◽  
Author(s):  
Ahmed S. Abu Zaid ◽  
Ahmed E. Aleissawy ◽  
Khaled Aboshanab ◽  
Mahmoud A. Yassien ◽  
Nadia A. Hassouna
Keyword(s):  
Ethyl Acetate ◽  
Large Scale ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Streptomyces Griseus ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
Scale Production ◽  
Bioassay Guided Fractionation ◽  
Diphenyl Tetrazolium Bromide

Abstract Natural products particularly microbial metabolites have been the mainstay of cancer chemotherapy and are likely to provide many of the lead structures and derivatives with new biological activities. In this research, the production of some potential cytotoxic metabolites from Streptomyces (S.) griseus isolate KJ623766 was carried out in 14 L laboratory fermenter under specified optimum conditions (28°C temperature, 200 RPM rotation speed, uncontrolled PH, 3 vvm aeration and 2 bar airflow pressure). After 72 hrs of incubation, the cell free culture supernatant (CFCS) was collected and extracted using ethyl acetate (1:1, v/v) at pH 7.0. Using 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium-bromide (MTT) assay, the cytotoxic activity of the ethyl acetate extract against Caco2 and Hela cancer cell lines was determined with CD50 of 14 µg/ml and 20 µg/ml, respectively. Bioassay guided fractionation of the ethyl acetate extract using different chromatographic techniques had led to the purification of the cytotoxic metabolites coded W1, R1 and R2 with reproducible amounts of 20, 5, and 1.5 mg/l, respectively. The structures of respective metabolites were determined based on the mass, 1D and 2D NMR spectroscopic analysis and were identified as genistein, β-rhodomycinone and γ- rhodomycinone, respectively. Accordingly, S. griseus isolate KJ623766 can be used as a potential industrial strain for the commercial production of the isoflavonoid genistein, as well as for the production of β-and γ- rhodomycinone to be used for the construction of new derivatives with more potent cytotoxic activities of the anthracycline family. To the best of our knowledge, this is the first report about the production of the isoflavonoid genistein by S. griseus KJ623766.

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