scholarly journals Antimalarial Activity of Sea Sponge Extract of Stylissa massa originating from waters of Rote Island

2021 ◽  
Vol 24 (4) ◽  
pp. 136-145
Author(s):  
Jefry Presson ◽  
Respati Tri Swasono ◽  
Sabirin Matsjeh ◽  
Meta Permata Putri ◽  
Zulfah Az Zahra ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Antimalarial Activity ◽  
Ethyl Acetate Fraction ◽  
Solvent Mixture ◽  
Extraction Yield ◽  
Unknown Compound ◽  
System Method

Research on the isolation, toxicity test, antimalarial test, and identification of the active compound from the ethyl acetate fraction of Stylissa massa sponge from Oenggae waters, Rote Island, has been conducted. This study aimed to investigate the antimalarial activity of the ethyl acetate fraction of the Stylissa massa sponge. Isolation was carried out by the extraction method using a mixed solvent of methanol: dichloromethane of 3: 2 (v/v), then the extract was partitioned in a solvent mixture of ethyl acetate: water of 1: 2 (v/v). The ethyl acetate extract obtained was separated by column chromatography using the gradient polarity system method. The toxicity test of each fraction was carried out by the Brine Shrimp Lethality Test (BSLT) method, and the antimalarial test was carried out by the haematin polymerization inhibition method. Identification of compounds from the active fraction in the antimalarial test was carried out using Liquid Chromatography-Mass Spectrometry (LC-MS). The extraction yield was 1.14 g (0.23%) of the ethyl acetate extract in the form of a dark brownish-yellow oily solid. Separation by column chromatography resulted in 15 fractions. Toxicity test results showed the four most active fractions with LC50 values, which are very promising for new drug discovery. The IC50 value in the antimalarial activity test of the four fractions indicated that the Stylissa massa sponge ethyl acetate extract was more active than the standard chloroquine compound (115 μg/mL). The LC-MS analysis indicates that fraction 11 contains two compounds that have been reported, and 1 compound is unknown. In contrast, fraction 14 indicates that it contains three compounds that have been reported and one unknown compound.

scholarly journals Isolation and Elucidation Structure of 28-Hydroxy-3-Friedelanone of Nyamplung (Callophyllum inophyllum, Linn.) Leaves

2020 ◽  
Vol 9 (2) ◽  
pp. 117-125
Author(s):  
Triana Kusumaningsih ◽  
◽  
Muhammad Widyo Wartono ◽  
Nais Puji Wijanarti ◽  
◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Nonpolar Solvent ◽  
Ether Extract ◽  
Diethyl Ether Extract ◽  
Isolated Compound

The isolation triterpenoid from Nyamplung (Callophyllum inophyllum, Linn.) leaves has been conducted. The isolation was employed by maceration using ethanol as solvent and liquids extraction using ethyl acetate. Ethyl acetate extract was partitioned successively using nonpolar solvent with hexane, dichloromethane, and diethyl ether, respectively. The diethyl ether extract was purified by column chromatography. The isolated compound of fraction D1 was obtained as white solids crystal with yield of 0.0035%. The isolated compound was determined based on the FTIR, 1HNMR, 13CNMR, HSQC, and HMBC spectra. The isolated compound was identified as 28-hydroxy-3-friedelanone.

scholarly journals Comparative phytotoxicity and chironomid toxicity assessments of leaf successive extract fractions of Asiatic poison bulb, Crinum asiaticum L

2020 ◽  
Author(s):  
Souren Goswami ◽  
Sanjib Ray
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Meristematic Tissue ◽  
Aqueous Fraction ◽  
Extract Fraction ◽  
Potent Growth ◽  
Crinum Asiaticum

AbstractCrinum asiaticum is an evergreen bulbous perennial shrub of Amaryllidaceae family with ethnomedicinal importance and our earlier study described a comparative account antimicrobial and antioxidant properties of the different solvent-mediated sequential extract fractions. The present study aimed to analyze their comparative account of induced phytotoxicity and chironomid toxicity. For phytotoxicity assessment, germination inhibition and seedling’s root and shoot growth retardation effects on Triticum aestivum and Cicer arietinum were analyzed and for lethal concentration determination, the freshwater bottom-dwelling first instars chironomid larvae were used. The crude aqueous, petroleum ether and ethyl acetate extracts showed significant toxic effects on both meristematic tissue and aquatic midges. The phytotoxic assays indicate that the ethyl acetate fraction contains the most potent growth inhibitors, followed by the crude aqueous and petroleum ether fractions. The last aqueous fraction was found to be the least toxic, the highest LC50 and LT50 values and ethyl acetate extract fraction having highest toxicity. Thus the present study supplements to our earlier report, that indicated the last aqueous extract fraction of C. asiaticum has potent antioxidant and antibacterial potentials as well as its prospective use in livestock maintenance, as it is least toxic and the ethyl acetate extract, the most toxic fraction identified here, is needed to explore for pharmaceutical importance.

scholarly journals ANTI DIABETIC FLAVANONE COMPOUND FROM THE LEAVES OF Artocarpus communis

2010 ◽  
Vol 9 (3) ◽  
pp. 505-508 ◽  
Author(s):  
Puspa D.N. Lotulung ◽  
Sofa Fajriah ◽  
Andini Sundowo ◽  
Euis Filaila
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Inhibition Assay ◽  
Spectroscopic Evidence

The Flavanone compound with anti diabetic activity was isolated from ethyl acetate extract of Artocarpus communis leaves using column chromatography techniques. The structure of the flavanone compound was elucidated on the basic of spectroscopic evidence and comparison to published values. This compound, 8-geranyl-4,5,7-trihydroxyflavone, showed strong anti diabetic activity on α-glucosidase inhibition assay with IC50 18.120 µg mL-1.   Keywords: Artocarpus communis, 8-geranyl-4,5,7-trihydroxyflavone, anti diabetic activity

scholarly journals Uji Efektivitas Bioherbisida Ekstrak Etil Asetat Babadotan (Ageratum conyzoides L.) Dari Berbagai Ketinggian Tempat Dan Konsentrasi Terhadap Pertumbuhan Gulma Bayam Duri (Amaranthus spinosus L.)

2020 ◽  
Vol 3 (4) ◽  
pp. 34-42
Author(s):  
Zainuddin Zainuddin ◽  
Siti Hafsah ◽  
Gina Erida
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
High Rate ◽  
Ageratum Conyzoides ◽  
Amaranthus Spinosus ◽  
Number Of Leaves ◽  
Materials Used ◽  
Banda Aceh ◽  
Ethyl Acetate Extracts

Abstrak. Penelitian ini bertujuan untuk menguji aktivitas ekstrak etil asetat babadotan dari berbagai ketinggian tempat dan konsentrasi terhadap pertumbuhan gulma bayam duri. Penelitian ini dilaksanakan di Laboratorium Ilmu Gulma Program Studi Agroteknologi dan Kebun Percobaan Fakultas Pertanian Universitas Syiah Kuala Banda Aceh, serta Laboratorium Analisis Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA), sejak September 2017– Januari 2018. Bahan yang digunakan adalah benih bayam duri, yang diperoleh dari Kecamatan Delima Kabupaten Pidie dan daun babadotan yang diperoleh dari 3 daerah dengan ketinggian yang berbeda yaitu Kecamatan Indrapuri Kabupaten Aceh Besar (19 mdpl), Kecamatan Lembah Seulawah Saree Kabupaten Aceh Besar (261 mdpl) dan Kecamatan Takengon Kabupaten Aceh Tengah (1259 mdpl) dengan konsentrasi 5, 10 dan 15% sebagai bioherbisida. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) pola faktorial 3  3 dengan 3 kali ulangan. Faktor pertama yaitu ketinggian tempat dengan 3 taraf dan faktor kedua konsentrasi dengan 3 taraf. Ekstrak yang dihasilkan pada ketinggian tempat yang berbeda dan konsentrasi ekstrak etil asetat berpengaruh nyata terhadap laju tinggi, jumlah daun, diameter batang, persentase pengendalian dan panjang akar. Terdapat interaksi antara ketinggian tempat dan konsentrasi terhadap persentase pengendalian pada 7 dan 14 HSA. Ekstrak yang dihasilkan pada ketinggian  9 mdpl dengan konsentrasi 10% dapat mengendalikan gulma bayam duri hingga 100% pada 7 HSA.The Test of Effectiveness Whiteweed Ethyl Acetate Extract (Ageratum conyzoides L.) from Various Altitudes And Concentration Against the Growth of Spiny Amaranth Weed (Amaranthus spinosus L.)Abstract. This study aims to examine the activity of ethyl acetate fraction of whiteweed extract from various altitudes and concentration againts the growth of spiny amaranth. This  research was conducted at Laboratory of Weed Science, Agrotechnology Department, and Experimental Garden, Agriculture Faculty, Syiah Kuala University Banda Aceh, and Laboratory of Chemical Analysis Mathematics Faculty and Natural Science, from September 2017 to January 2018. The materials used is spiny amaranth seed that obtained from Subdistrict of Delima, District of Pidie and babadotan leaves obtained from three regions with different altitude that is Subdistrict of Indrapuri, District of Aceh Besar (19 masl), Subdistrict of Subdistrict of, District of Aceh Besar (261 masl) and Subdistrict of Takengon District of Aceh Tengah (1259 masl) with each concentration 5, 10 and 15% as bioherbicide. This research using Randomized Completely Design with Factorial pattern which has 3 × 3 treatments and 3 replication. The first faktor is altitude and second is concentration of ethyl acetate extract. Ethyl acetate extracts that produced in different altitudes and concentrations were significantly influence the high rate of spiny amaranth, number of leaves, stem diameter, control percentage and root length. There is an interaction between altitude and concentration on the percentage of control at 7 and 14 HSA. Extracts produced at an altitude of 9 masl with a concentration of 10% can control spinach weeds up to 100% at 7 HSA.

scholarly journals Isolasi Senyawa Kimia Stigmastan-3,5-Diena Yang Mempunyai Daya Toksik Dari Daun Ekaliptus (Eucalyptus Deglupta Blume.)

2017 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Sari Setianingsih ◽  
Rudi Kartika ◽  
Partomuan Simanjuntak
Keyword(s):  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Test Results ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Ms Analysis

This study was started by extraction of Eucalyptus deglupta Blume. Using organic solvent   (n-hexane, ethyl acetate, ethanol and water) followed by phytochemical screening and toxicity test using Brine Shrimp Lethality Test (BSLT) method. Isolation and identification of chemical compounds contained in the fraction were done by column chromatography and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Phytochemical screening revealed the presence of alkaloids, flavonoids, steroids and phenolics in the extract. Toxicity test results showed that the ethyl acetate extract was potentially active with LC50 value of  617.95 ppm. The extract was continued to isolation stage and gave fraction EKEA-3.1 with LC50 value of 2759.93 ppm. Identification of chemical compounds in EKEA-3.1 with KG-MS analysis showed that EKEA-3.1 was suspected to be Stigmastan-3,5-diene.

scholarly journals Antimalarial Activity of Ethyl Acetate Extract and Fraction of Bidens pilosa against Plasmodium berghei (ANKA)

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Noumedem Anangmo Christelle Nadia ◽  
Yamssi Cédric ◽  
Simeni Njonnou Sylvain Raoul ◽  
Ngongang Ouankou Christian ◽  
Mounvera Abdel Azizi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Crude Extract ◽  
Plasmodium Berghei ◽  
Ethyl Acetate Extract ◽  
Antimalarial Activity ◽  
Hematological Parameters ◽  
Control Group ◽  
Bidens Pilosa

Background. Malaria is one of the most critical diseases causing about 219 million cases worldwide in developing countries. The spread and development of resistance against chemical antimalarial drugs is one of the major problems associated with malaria control. The present study was to investigate the antimalarial efficacy of ethyl acetate extract and one fraction of Bidens pilosa in vivo in order to support the usage of this plant by traditional healers to treat malaria. Methods. The extracts were prepared by maceration of B. pilosa leaf powder in ethyl acetate. The liquid filtrate of the extract and the best in vitro antiplasmodial fraction using HPLC were concentrated and evaporated using a rotavapor under vacuum to dryness. The antimalarial activity of B. pilosa plant products were evaluated in vivo against Plasmodium berghei infected mice according to the Peter and Rane test. The antimalarial efficacy of the a selected crude extract (ethyl acetate extract) was evaluated at 125, 250, and 500 mg/kg, while a selected fraction from ethyl acetate extract (fraction 12) was evaluated at 62.5 and 125 mg/kg. Blood from experimental animals was collected to assess hematological parameters. Results. The crude extract of ethyl acetate and fraction 12 demonstrated 100% in vivo parasite suppressive activity at doses of 500 mg/kg and 125 mg/kg, respectively, for the crude extract and fraction 12. The mice treated with 250 and 500 mg/kg had their parasitemia (intraerythrocytic phase of P. Berghei) drop considerably, disappearing by the 8th day in mice receiving 500 mg/kg. The ethyl acetate extract of B. pilosa, fraction 12 showed an even higher antiplasmodial activity. By the 5th day of the experiment, the treatment led to a modification of hematological parameters in mice. The chloroquine (5 mg/kg), fraction 12 (125 mg/kg), and the crude extract (500 mg/kg) groups all survived the 30 days of the experiment, while the negative control group registered 100% of the deaths. Conclusion. This study scientifically supports the use of Bidens pilosa leaves in the traditional treatment of malaria. However, the mode of action and in vivo toxicity of the plant still need to be assessed.

scholarly journals ANTIOXIDANT ACTIVITY OF 2,6,4’-TRIHYDROXY-4-METHOXY BENZOPHENONE FROM ETHYL ACETATE EXTRACT OF LEAVES OF MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.)

2011 ◽  
Vol 11 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Susilawati Susilawati ◽  
Sabirin Matsjeh ◽  
Harno Dwi Pranowo ◽  
Chairil Anwar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Column Chromatography ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Chemical Constituent ◽  
Traditional Medicines ◽  
Phaleria Macrocarpa ◽  
Spherical Crystal

Mahkota dewa plant (Phaleria macrocarpa (Scheff.) Boerl.) which is included into family of Thymelaeaceae is one of Indonesia's traditional medicines. Chemical constituent has been isolated from ethyl acetate extract of leaves of mahkota dewa. Sample was extracted with methanol, concentrated then extracted by n-hexane, chloroform and ethyl acetate. The ethyl acetate extract was separated and fractionated by column chromatography. The first fraction was purified by TLC preparative and recrystalization. Compound was isolated as red-brown spherical crystal in 8 mg (m.p. 129-131 °C). Its spot gave dark fluoroscence at TLC plate (UV366) with Rf of 0.3 at TLC chromatogram with eluent of n-hexane : ethyl acetate (7:3); 0.6 with n-hexane : ethyl acetate (1:1); 0.9 with -hexane : ethyl acetate (4:6). This compound was dissolved in methanol. Compound was identified by UV, IR, 1H NMR, 13C NMR and NMR 2 dimension (HMQC, COSY, HMBC and DEPT-135) spectroscopic as 2,6,4'-trihydroxy-4-methoxybenzophenon. This compound as well as the ethyl acetate extract showed antioxidant activity on DPPH with IC50 was 10.57 and 101.06 μg/mL, respectively. This compound showed strong antioxidant activity on DPPH, almost to the standard antioxidant activity of quercetin (IC50 of 2.93 μg/mL)

scholarly journals Uji Fitokimia dan Toksisitas Ekstrak Etil Asetat Kulit Batang Melochia umbellata (Houtt) Stapf. var. Visenia dengan Metode Brine Shirmp Lethality Test (BSLT)

2017 ◽  
Vol 4 (2) ◽  
pp. 378-381 ◽  
Author(s):  
Fauziah Ahmad ◽  
Nunuk H. Soekamto ◽  
Firdaus Firdaus
Keyword(s):  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Artemia Salina ◽  
Bark Extract ◽  
Steroid Compound ◽  
Value Analysis ◽  
South Sulawesi ◽  
The People ◽  
Test Result

Melochia umbellata (Houtt) Stapf. var. Visenia is plant species included in Malvaceae family. This spesies is known as paliasa and used as traditional medicine by the people of South Sulawesi. This study aimed to determine the secondary metabolites by reagents and toxicity characteristic testing from bark extract of Melochia umbellata (Houtt) Stapf. var. Visenia using Brine Shirmp Lethality Test (BSLT) method. The extract was prepeared by maceration ethyl acetate. Phytochemical test result showed that the ethyl acetate extract was containing the steroid compound, alkaloids and terpenoids. Toxicity test performed by shrimp Artemia salina Leach larvae was at 48 hours. The toxic effects of the extract were identified by the percentage of the number of shrimp larvae mortality using probit value analysis (LC50). The toxicity test of bark Melochia umbellata (Houtt) Stapf. var. Visenia extract showed that the ethyl acetate extract is toxic to A. salina  LC50 = 54,55 mg / mL.

scholarly journals Detection of bleomycin- like antitumor agent produced by Streptomyces spp. local isolates

2007 ◽  
Vol 1 (1) ◽  
pp. 15-26
Author(s):  
Asia J. Rweish ◽  
Mohammed A. K. Ibrahim ◽  
Rafid A. Abdul-karim ◽  
Mohammed R. Abdul-majeed ◽  
Zahra K. Jawad
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Fermentation Broth ◽  
Antitumor Agent ◽  
Moderate Activity ◽  
Gram Negative Bacteria ◽  
Unknown Compound ◽  
Streptomyces Spp ◽  
Antitumor Compounds ◽  
Ethyl Acetate Extracts

Ethyl acetate extracts of fermentation cultures of five local Streptomyces isolates SRY-3, SRY-25, 5b, 10, NS-38 were identified as inhibitors of plasmacytoma cell line. Concentration at 0.016 µg/ml of ethyl acetate extract of (SRY-3,SRY-25,5b,10,NS-38) inhibited (46%,40%,21%,34%,34%) of mouse plasmacytoma cells respectively, where as 0.5 µg/ml inhibited (58%, 55%, 35%, 33%, 36%) of plasmacytoma cells .Quantities analysis of crude extracts by using HPLC on the basis of their retention times showed that the values were (4.3,4.4,4.86,4.83&4.84) min. for SRY-3, SRY-25, 5b, 10,NS-38 respectively, while the retention time of standard antitumor compounds was (4.26) min. This suggests that unknown compound of SRY-3 extract contain bleomycin-like compound. Addition of standard bleomycin to crude extract of SRY-3 increased milliabsorbans unit (M.A.U.) from 0.49 to 1.4. The concentration of bleomycin-like antitumor in fermentation broth of SRY-3 isolate was 3.441 µg/ml.The five Streptomyces isolates have moderate activity against gram-positive and gram-negative bacteria, while standard BLM have no activity.

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