scholarly journals IDENTIFICATION AND ANTIMICROBIAL SUSCEPTIBILITY TESTING OF PATHOGENIC MICRO-ORGANISM FROM DENTAL PATIENTS

2016 ◽  
pp. 226
Author(s):  
Sakthivel M ◽  
Ayyasamy Pm ◽  
Arvind Prasanth D
Keyword(s):  
Periodontal Diseases ◽  
Antimicrobial Property ◽  
Isolation And Identification ◽  
Dental Patients ◽  
Periodontal Bacteria ◽  
Number Of Patients ◽  
Study Population ◽  
Oral Microbes ◽  
Effective Drugs ◽  
Dental Infections

<p>ABSTRACT<br />Objective: To isolate and identify aerobic microbes present in the periodontal infected patients and to evaluate the choice of antibiotics in the<br />management of periodontal diseases.<br />Methods: In this study, these patients have not been treated previously for their conditions. An informed consent was obtained from these patients<br />before collection of an oral swab. This study was approved by the Institutional Ethical Committee. The details of the patient’s age, sex, and clinical<br />details were recorded on a per forma meant for this study. The following methodologies were adopted for the isolation and identification of the microorganisms<br />from<br />these cases.<br />Results: In this study out of 50 oral samples, culture positivity was recorded in 43 (86%) cases and no growth in 7 (14%) cases. Antibiotic susceptibility<br />test using to identified as resistant, sensitive, intermediate of pathogenicity of oral microbes. Such antibiotics were methicillin, ceftazidime,<br />clindamycin, amikacin, cloxacillin, and cefotaxime. This study should be kept in mind when a local application of antibacterial compounds is used in<br />the therapy of periodontal disease.<br />Conclusion: This study highlights the different organisms involved in the different types of dental infections. The antibiotic pattern shown in this work<br />will be a guide to the clinician in the selection of proper antibiotics for the treatment of these infections. Hence in this study, the limitations were time<br />and the number of patients. For better outcomes, a larger study population for a longer period of time should be undertaken to know the bacteriology<br />and to the select the effective drugs of choice for dental infections. A comparative study of bacteriology and mycology and its antimicrobial property<br />would be very fruitful in the future.<br />Keywords: Dental, Periodontal, Bacteria, Antibiotics.</p>

Comparative analysis of polymorphic variants of IL-17A and MMP-1 genes with the risk of developing chronic periodontitis in petrochemical workers

2020 ◽  
Vol 60 (10) ◽  
pp. 687-693
Author(s):  
Iskander I. Zaidullin ◽  
Denis O. Karimov ◽  
Lilija K. Karimova ◽  
Milyausha F. Kabirova ◽  
Rasima R. Galimova ◽  
...  
Keyword(s):  
Ethylene Oxide ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Clinical Manifestations ◽  
Control Group ◽  
Periodontal Tissues ◽  
Dental Examination ◽  
Number Of Patients ◽  
Increased Risk ◽  
Harmful Substances

The susceptibility to the development and progression of inflammatory periodontal diseases, which depends on genetic and external factors (smoking, stress, oral hygiene), varies widely. In the development of these diseases, an important role is played not only by the presence of periodontal pathogenic microorganisms, but also by the presence of congenital or acquired immunodeficiency, immunoregulatory defects. The immune system plays a key role in the physiological and pathological processes of periodontal tissues. In this regard, IL17, produced by CD4+ Th cells, which has both Pro-inflammatory and protective activity, is of particular interest in the pathogenesis of periodontitis. The aim of study was to identify the relationship between polymorphic loci of the IL-17A (rs2275913) and MMP-1 (rs1799750) genes and clinical manifestations of chronic periodontitis in petrochemical workers. Dental examination was performed in 92 ethylene oxide production workers with chronic periodontitis and 74 patients with chronic periodontitis who did not come into contact with chemical factors (control group). Genotyping of polymorphisms rs2275913 of the IL17A gene and rs1799750 of the MMP1 gene was performed by allele-specific real-time polymerase chain reaction (PCR). Hygienic assessment of the degree of air pollution of the working area with harmful substances was carried out by gas chromatography according to the guidelines for the determination of harmful substances in the air № 5098-89, № 3119-84. When comparing the results of studies of both groups, there were no statistically significant differences in the frequency distributions of allelic variants and genotypes of the IL-17A and MMP-1 genes. The AA/AG genotypes of the IL-17A gene were associated with an increased risk of severe disease compared to the GG genotype in workers in the main group (OR=6.1; 95% CI 1.33-28.5; p=0.021) and in the control group (OR=7.26; 95% CI 1.34-39.25; p=0.016). Carriers of the A allele in the control group increased the risk of severe chronic periodontitis by 2.4 times compared to carriers of the G allele (OR=2.41; 95% CI 1.19-4.87; p=0.014). During the dental examination of employees of the ethylene oxide plant, the clinical course of periodontal diseases was more severe in comparison with the control group, and the number of patients with severe periodontitis was twice as high. It was found that the AA/AG genotypes of the IL-17A gene and the carrier of the A allele are associated with increased susceptibility to the development of severe chronic periodontitis. The association between the MMP-1 gene polymorphism and the risk of severe forms of chronic periodontitis has not been established. A risk factor for the development of inflammatory periodontal diseases in employees of the petrochemical complex is a complex of harmful production factors.

scholarly journals Presence of Periodontopathic Bacteria DNA in Atheromatous Plaques from Coronary and Carotid Arteries

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Malgorzata Szulc ◽  
Wojciech Kustrzycki ◽  
Dariusz Janczak ◽  
Dagmara Michalowska ◽  
Dagmara Baczynska ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Carotid Arteries ◽  
Periodontal Diseases ◽  
Vascular Diseases ◽  
Bacterial Dna ◽  
Chronic Inflammatory Response ◽  
Atheromatous Plaques ◽  
Patients At Risk ◽  
Study Population ◽  
Artery Disease

Objectives. Interest in periodontitis as a potential risk factor for atherosclerosis and its complications resulted from the fact that the global prevalence of periodontal diseases is significant and periodontitis may induce a chronic inflammatory response. Many studies have analyzed the potential impact of thePorphyromonas gingivalis, major pathogen of periodontitis, on general health. The purpose of this study was to find the presence of thePorphyromonas gingivalisDNA in the atherosclerotic plaques of coronary and carotid arteries and in the periodontal pockets in patients with chronic periodontitis, who underwent surgery because of vascular diseases.Methods and Results. The study population consisted of 91 patients with coronary artery disease or scheduled for carotid endarterectomy. The presence ofPorphyromonas gingivalisDNA in atheromatous plaques and in subgingival samples was determined by PCR. Bacterial DNA was found in 21 of 91 (23%) samples taken from vessels and in 47 of 63 (74.6%) samples from periodontal pockets.Conclusions.Porphyromonas gingivalisDNA is frequently found in atheromatous plaques of patients with periodontitis. That is why more research should be conducted to prove if this periopathogen may have an impact on endothelium of patients at risk of atherosclerosis.

scholarly journals Anti-inflammatory activity of Punica granatum extract on oral microbes - In vitro

2019 ◽  
Vol 10 (2) ◽  
pp. 1019-1022
Author(s):  
Westeros Dominic Pereira ◽  
Geetha RV ◽  
Lakshmi Thangavelu
Keyword(s):  
Protein Denaturation ◽  
Periodontal Diseases ◽  
Punica Granatum ◽  
Inflammatory Activity ◽  
Oral Diseases ◽  
Major Health Problem ◽  
Maximum Inhibition ◽  
Anti Inflammatory ◽  
Oral Microbes

To study the anti-inflammatory effect of Punica granatum extract against the oral microbes. Oral diseases continue to be a major health problem worldwide. Dental caries and periodontal diseases are among the most important global oral health problems, although conditions such as oral and pharyngeal cancers and oral tissue lesions are also significant health concerns. Pomegranate extracts have been used for centuries in traditional medicine to confer health benefits in a number of inflammatory diseases, microbial infections and cancer. The anti-inflammatory activity of pomegranate extract was evaluated by protein denaturation assay, and the results were read spectrophotometrically. Denaturation of proteins is a great‐ documented cause of inflammation. As a part of the investigation on the mechanism of the anti-inflammatory activity, the ability to extract to inhibit protein denaturation was studied. It was effective in inhibiting heat induced albumin denaturation at different concentrations as shown in Table 1. Maximum inhibition, 70.12±1.12% was observed at500µg/ml. IC50 value was found to be 105.35±1.99µg/ml. Aspirin, a standard anti-inflammatory drug showed the maximum inhibition, 77.12±1.42% at the concentration of 200µg/ml. Hence it can be concluded that pomegranate extract has anti-inflammatory property and also can be used in products such as toothpaste and mouth wash etc.

scholarly journals The prevalence of gastroesophageal reflux disease and its association with various risk factors in a tertiary care centre in West Bengal

2021 ◽  
Vol 9 (3) ◽  
pp. 780
Author(s):  
Anant Parasher ◽  
Kunal Ranjan
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Reflux Disease ◽  
Urban Areas ◽  
West Bengal ◽  
Past History ◽  
Tertiary Care ◽  
Time Interval ◽  
Number Of Patients ◽  
Study Population

Background: Gastroesophageal Reflux (GER) is the regurgitation of gastric contents and acid into the esophagus. Frequent and abnormal amounts of reflux leads to Gastroesophageal reflux disease (GERD) which causes symptoms like heartburn, regurgitation, and /or other complications. In view of absence of any data on the prevalence of GERD from this part of India, the current population based study was conducted to study the prevalence of GERD and its association with various risk factors.Methods: In this community based prospective cross-sectional observational study, 500 patients from rural and urban areas of West Bengal were included during the one year period from July 2014 to June 2015.Results: Out of a total of 500 patients studied, the percentage of patients with GERD in our study population was found to be 31.3%. Out of 292 males enrolled for the study, number of patients with GERD was 66 (22.6%) as compare to females where the same was found to be 43.26% (90/208). The majority (68.2%) of patients had mild GERD, 18.5% had moderate GERD whereas only 13.3% of patients had severe GERD.Conclusions: The percentage of patients with GERD in our study population was found to be 31.3%. It was observed to be significantly associated with increasing Body Mass Index (B.M.I), smoking, the female gender, a sedentary lifestyle, dinner to bed-time interval of ≤2 hours, chronic NSAID use, and a past history of abdominal surgery.

scholarly journals Screening of Punica granatum extract for antimicrobial activity against oral micro organisms

2020 ◽  
Vol 6 (2) ◽  
pp. 73-77
Author(s):  
Meera Avadhani ◽  
◽  
Meena Anand Kukkamalla ◽  
Kishore G Bhat ◽  
◽  
...  
Keyword(s):  
Research Work ◽  
Fusobacterium Nucleatum ◽  
Punica Granatum ◽  
Dilution Method ◽  
Dental Patients ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Serial Dilution Method ◽  
Micro Organisms ◽  
Dental Infections

Background and Objectives: A lot of research work in both dental and medical fields support the curative properties of pomegranate. Accordingly, it was decided to prepare a pomegranate mouthwash and evaluate it among Dental patients diagnosed with Chronic Gingivitis. The objective of the present invitro study is to assess the Minimum Inhibitory Concentration (MIC) of the commercially available pomegranate extract powder against few oral pathogenic microorganisms. Methodology: Serial dilution method using thioglycolate broth medium was used for anerobes like Streptococcus mutans, Fusobacterium nucleatum, Aggregatibacter actinomycetomcomitans, Prevotella intermedia and Mueller hinton agar mediated growth was used for aerobe like Staphylococcus aureus. Following which microdilution assay was performed and accordingly evaluated the MIC. Based on this report, the test rinse was prepared and further evaluated using the same methodology for both aerobes and anerobes. Results and Inference: It was observed from the MIC report for both aerobes and anerobes that at a concentration of 0.2% the formulated mouth rinse was effective against all the chosen organisms. The results of the study infer that products like mouthwash, dental gels etc made from this concentration could be possibly used for the control of dental infections.

ROLE OF AMBROXOL AS A PROPHYLACTIC AGENT AGAINST COVID-19

2021 ◽  
Author(s):  
Azza M. Baraka ◽  
Wessam F. El-Hadidy
Keyword(s):  
Peer Review ◽  
Serine Protease ◽  
Periodontal Diseases ◽  
Antiviral Drug ◽  
Acceptable Solution ◽  
Dental Patients ◽  
Dental Practitioners ◽  
Molar Incisor Hypomineralization ◽  
Approved Drugs ◽  
Transmembrane Serine Protease

 Currently the world is facing a pandemic disease, namely Coronavirus disease 2019 (COVID-19) that is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). As finding recent drugs targeting COVID-19 will take a long time, so repositioning currently existing FDA approved drugs for treating this disastrous disease is an acceptable solution. It has been found that for SARS-CoV-2 to be infective, this necessitates splitting of the viral spike glycoproteins by the serine protease “type II transmembrane serine protease TMPRSS2” that has shown to be widely expressed in pulmonary tissues. Thus, TMPRSS2 is suggested to be potential target for antiviral drug design against COVID-19.  The mucokinetic drug “Ambroxol” has been reported as a potent inhibitor of TMPRSS2, thus it could represent a therapeutic as well as a prophylactic candidate against SARS-CoV2. This review gives a brief summary about ambroxol’s potential role against COVID-19’s TMPRSS2.                            Peer Review History: Received 8 January 2021; Revised 5 February; Accepted 25 February, Available online 15 March 2021 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 5.5/10 Average Peer review marks at publication stage: 7.0/10 Reviewer(s) detail: Dr. Nuray Arı, Ankara University, Turkiye, [email protected] Prof. Cyprian Ogbonna ONYEJI, Obafemi Awolowo University, Ile-Ife, Nigeria, [email protected] Dr. Asia Selman Abdullah, Al-Razi university, Department of Pharmacy, Yemen, [email protected]  Similar Articles: RISK FACTORS OF PERIODONTAL DISEASES AMONG YEMENI YOUNG DENTAL PATIENTS KNOWLEDGE AND PERCEPTION OF MOLAR INCISOR HYPOMINERALIZATION AMONG DENTAL PRACTITIONERS IN SANA’A CITY- YEMEN

scholarly journals Errata

1992 ◽  
Vol 7 (2) ◽  
pp. 185-185
Keyword(s):  
Disaster Medicine ◽  
Adult Patients ◽  
Typographical Error ◽  
Tracheal Lumen ◽  
Number Of Patients ◽  
Study Population

In the article, “Multi-Agency, Prehospital Evaluation and the Pharyngeo-Tracheal Lumen (PTL) Airway,” by McMahan, Ornato, Racht, and Cameron, on pages 13–16, in January–March 1992 issue of Prehosptal and Disaster Medicine, there was a typographical error regarding the number of patients in the study population. On page 13, the Methods section of the Abstract incorrectly states that 1,647 adult patients participated in the study. The actual study population was 167 patients. We regret the error.

scholarly journals Total hip arthroplasty for sickle cell osteonecrosis: guidelines for perioperative management

2020 ◽  
Vol 5 (10) ◽  
pp. 641-651
Author(s):  
Philippe Hernigou ◽  
Victor Housset ◽  
Jacques Pariat ◽  
Arnaud Dubory ◽  
Charles Henri Flouzat Lachaniette
Keyword(s):  
Total Hip Arthroplasty ◽  
Sickle Cell ◽  
Hip Arthroplasty ◽  
Perioperative Management ◽  
Senior Author ◽  
Clinical Expertise ◽  
Cell Disease ◽  
Total Hip ◽  
Number Of Patients ◽  
Study Population

The prognosis of sickle cell disease (SCD) has greatly improved in recent years, resulting in an increased number of patients reporting musculoskeletal complications such as osteonecrosis of the femoral head. Total hip arthroplasty (THA) can be utilized to alleviate the pain associated with this disease. Although it is well known that hip arthroplasty for avascular necrosis (AVN) in SCD may represent a challenge for the surgeon, complications are frequent, and no guidelines exist to prevent these complications. Because patients with SCD will frequently undergo THA, we thought it necessary to fulfil the need for guidance recommendations based on experience, evidence and agreement from the literature. For all these reasons this review proposes guidelines that provide clinicians with a document regarding management of patients with SCD in the period of time leading up to primary THA. The recommendations provide guidance that has been informed by the clinical expertise and experience of the authors and available literature. Although this is not a systematic review since some papers may have been published in languages other than English, our study population consisted of 5,868 patients, including 2,126 patients with SCD operated on for THA by the senior author in the same hospital during 40 years and 3,742 patients reported in the literature. Cite this article: EFORT Open Rev 2020;5:641-651. DOI: 10.1302/2058-5241.5.190073

Stress and the periodontal diseases: growth responses of periodontal bacteria to Escherichia coli stress-associated autoinducer and exogenous Fe

2005 ◽  
Vol 20 (3) ◽  
pp. 147-153 ◽  
Author(s):  
A. Roberts ◽  
J. B. Matthews ◽  
S. S. Socransky ◽  
P. P. E. Freestone ◽  
P. H. Williams ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Periodontal Diseases ◽  
Growth Responses ◽  
Periodontal Bacteria

scholarly journals Role of Communication, Professionalism, and Clinical Care Skills of Postgraduate Students on Patients Recall Visits in Dental School—An Observational Study

2020 ◽  
Vol 7 (6) ◽  
pp. 1563-1567
Author(s):  
Mohan Kumar Pasupuleti ◽  
Gautami Subhadra Penmetsa ◽  
Meghana Gangolu ◽  
Santosh Venkata Ramesh Konathala ◽  
Sruthima Naga Venkata Satya
Keyword(s):  
Periodontal Diseases ◽  
Clinical Care ◽  
Periodontal Health ◽  
Postgraduate Students ◽  
Dental Patients ◽  
Effective Evaluation ◽  
Clinical Encounters ◽  
Dental Schools ◽  
New Criteria

Preservation of periodontal health after periodontal therapy is paramount for the complete elimination of periodontal diseases. In most of the Dental Schools, recall appointments are considerably low, and in particular, to the Department of Periodontics, the compliance to recall visits by the patients diagnosed with periodontal disease is still inadequate. Faculty from the Department of Periodontics framed new criteria to follow in the comprehensive clinics by the postgraduate students. The criteria include communication, intraoral examination skills, and professionalism toward dental patients. Faculty in each comprehensive dental clinic observed the clinical encounters of postgraduate students with patients and provided the feedback. The study conducted from May 2018 to April 2019, and the patients attended were 1164 of 1544. Unattended patients were 380 of 1544. Effective evaluation of the feedback provided by faculty and communication with both the postgraduate students and unattended patients resulted in further improvement in recall, that is, 151 of 380 patients. Reframed criteria, direct observation of the postgraduate students by faculty during patients interaction, assessment of feedback forms, and immediately modifying the student’s way of communication allowed maximum recall visits to the Department of Periodontics.

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