scholarly journals IN VITRO ANTIOXIDANT ACTIVITY OF PHYLLODIUM PULCHELLUM L. DESV - AN THREATENED MEDICINAL PLANT

2017 ◽  
Vol 10 (10) ◽  
pp. 282
Author(s):  
Gopal Murugan Velmurugan ◽  
Subramaniam Parvathi Anand
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Threatened Medicinal Plant ◽  
In Vitro Antioxidant Activity

  Objectives: In this study, we determined the in vitro antioxidant capacity of Phyllodium pulchellum of aqueous, ethanol, and chloroform leaf extracts.Methods: In this context, the in vitro antioxidant activity was demonstrated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis(3- ethylbenzothiazolone-6-sulfonic acid) (ABTS+) radical scavenging assay, the total antioxidant activity of phosphomolybdenum assay and hydroxyl radical scavenging activity in different leaf extracts of P. pulchellum. The antioxidant activity of the extracts was compared to standard ascorbic acid.Results: All the four methods of antioxidant showed good reducing power and reducing capacity with increasing concentration again taking the ethanol leaf extract to the top position. Remarkable of antioxidant activity was observed in ethanol leaf extract on the hydrogen peroxide scavenging activity with the lowest inhibitory concentration 50 values of (155.40 μg/ml) followed by DPPH (432.90 μg/ml) and ABTS+ (524.40 μg/ml).Conclusion: These results suggest that the leaf of P. pulchellum could be a valuable source of new antioxidant properties, from the above results it seen that this plant exhibits pharmaceutical activity. 

scholarly journals Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

2020 ◽  
Vol 11 (4) ◽  
pp. 6262-6267
Author(s):  
Krishnamoorthy Meenakumari ◽  
Giridharan Bupesh ◽  
Mayur Mausoom Phukan
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Properties ◽  
Scavenging Activity ◽  
Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

scholarly journals FREE-RADICAL SCAVENGING ACTIVITY LEAF EXTRACT OF LITSEA LAEVIGATA GAMBLE

2019 ◽  
pp. 96-103
Author(s):  
S. SUJATHA ◽  
T. SEKAR
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Metal Chelating

Objective: In the present study, antioxidant activity in the leaf of the pet-ether, chloroform, acetone and methanolic extracts from Litsea laevigata Gamble. Leaf was investigated by employing established in vitro studies. L. laevigata belongs to the Lauraceae family. Methods: The capability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals were determined by in vitro antioxidant assays using 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH.) scavenging, reducing power assay, superoxide radical (O2*-) scavenging activity, phosphomolybdenum assay, FRAP, ABT and metal chelating activity were performed to know the antioxidant potency of the plant extract of leaves of L. laevigata. Results: Results are evaluated higher in leaf extract of L. laevigata recorded total phenol, total flavonoid, and tannin. The present state of work was designed to evaluate the phytochemical, antioxidant in the plant leaf extracts of L. laevigata. The plant L. laevigata methanolic extract of leaf showed greater IC50 antioxidant activity of DPPH assay (5.264 µg/ml) and compare to other extract, higher phosphomolybdenum reduction (164.36 mg/g), better Reducing power activity leaf in methanol (0.711%), higher ferric reducing power (4060.66MmolFe(II)E/mg), and higher in superoxide radical scavenging activity in (78.12 mg/ml). However, the better metal chelating ability was shown by the water extracts of the leaf (5.145 EDTAE/100g) compared to other solvent extracts. Conclusion: The result indicates the total phenol and antioxidant activity potential of L. laevigata.

scholarly journals Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

2017 ◽  
Vol 71 (5) ◽  
pp. 361-370
Author(s):  
Slavica Grujic ◽  
Ana Dzamic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
P.D. Marin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

Radical Scavenging Activities of Heracleum aquilegifolium Wight (Apiaceae) Fruit Oils in vitro

2010 ◽  
Vol 65 (11-12) ◽  
pp. 653-659 ◽  
Author(s):  
Subbiah Karuppusamy ◽  
Gurunathan Muthuraja
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Butylated Hydroxytoluene ◽  
Scavenging Activity ◽  
Nitric Oxide Radical

The fruits of Heracleum aquilegifolium Wight (Apiaceae) were collected from Western Ghats of the Indian Peninsula. The essential oils were extracted by hydrodistillation. The chemical composition of the essential oils was analysed by gas chromatography and gas chromatography-mass spectrometry (GC-MS). β -Pinene (22.3%), 1,8-cineole (20.3%), and β-phellandrene (12.4%) were the main components of H. aquilegifolium fruit oils. The antioxidant properties of essential oils of H. aquilegifolium were examined by different procedures namely reducing power ability, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, nitric oxide radical scavenging activity, hydrogen peroxide scavenging activity, hydroxyl radical scavenging activity, superoxide anion scavenging activity, and metal chelating activity. The antioxidant activities were compared with those of synthetic antioxidants and standard drugs such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), ascorbic acid, α-tocopherol, curcumin, and quercetin. The study confirmed the possible antioxidant potential of essential oils tested with various in vitro antioxidant methods. The presence of monoterpenes in combination with other components in the oils could be responsible for the activity

scholarly journals Antioxidant Capacities of Jujube Fruit Seeds and Peel Pulp

2020 ◽  
Vol 10 (17) ◽  
pp. 6007 ◽  
Author(s):  
Yung-Sheng Lin ◽  
Wen-Shin Lin ◽  
Jing-Wen Tung ◽  
Ya-Chih Cheng ◽  
Min-Yun Chang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Jujube Fruit

In this study, the effects of different fruit parts and extraction conditions on the antioxidant properties of jujube (Ziziphus jujuba Mill.) fruit were investigated. Five in vitro antioxidant models and statistical analyses were performed. The results revealed that jujube peel with pulp (peel pulp) exhibited better antioxidant capacity than did seeds. Overall, jujube peel pulp extracted using 50% ethanol at 60 °C exhibited the best antioxidant capacity in terms of 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (0.3 ± 0 mg/mL), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity (0.5 ± 0 mg/mL), total phenolic content (38.3 ± 0.4 mg gallic acid equivalent per gram dry weight), total flavonoid content (43.8 ± 0.2 mg quercetin equivalent per gram dry weight), and reducing power (41.9 ± 2.2 mg ascorbic acid equivalent per gram dry weight). The results indicated that jujube peel pulp is a more potential natural antioxidant than seeds.

scholarly journals Discovery of Some Emerging Free Radical Scavenging Candidates Bearing 2-(P-hydroxyphenyl)-4–(Substitutedphenyl)-1H-1,5–Benzodiazepines Pharmacophore

2017 ◽  
Vol 9 (04) ◽  
Author(s):  
Animeshchandra G. M. Haldar ◽  
Santosh S. Chhajed ◽  
Debarshi Kar Mahapatra ◽  
Debarshi Kar Mahapatra
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Analytical Techniques ◽  
Free Radical Scavenging ◽  
Free Radical Scavengers ◽  
Scavenging Activity ◽  
In Vitro Antioxidant Activity

In the present investigation, the synthesis of few novel leads bearing 2-(p-hydroxyphenyl)-4–(substitutedphenyl)-1H-1,5–benzodiazepine pharmacophore is described. The substituted chalcone and their derivatives 3(a-j) were synthesized by base catalyzed Claisen-Schmidt condensation between p-hydroxy-acetophenone and appropriate aldehydes. The dibromostyryl ketones 4(a-j) were obtained by the reaction the chalcone with bromine in acetic acid. The dibromostyryl ketone were reacted with methanol in presence of sodium methoxide followed by acidic hydrolysis give 1-(4-hydroxyphenyl)-3-(substitutedphenyl)-1,3-propanediones. The targeted compounds; the substituted 1,5-benzodiazepines were synthesized with o-phenylenediamine and synthesized 1,3-propanediones. The structures of synthesized compounds were confirmed by spectroscopic and analytical techniques (IR, 1H-NMR, and MS). The free radical scavenging activity of the synthesized analogs was monitored by in vitro antioxidant activity protocol. The derivatives 6f, 6g, 6i, and 6j were found to exhibit good antioxidant activity with 59.07%, 41.33%, 68.3% and 60.4% scavenging activity respectively as compared to standard ascorbic acid which demonstrated 79.73% activity. The current research revealed the potential of 2-(p-hydroxyphenyl)-4–(substituted-phenyl)-1H-1,5–benzodiazepine as emerging free radical scavengers. The study helped to establish a structure-activity relationship (SAR) where the substitution on the phenyl moiety of the 1,5-benzodiazepine was found to play profound role and influence over biological activity. The research will open new avenues for the development of antioxidant moieties having perspectives in cancer, inflammation, and several other ailments.

scholarly journals Synthesis and in Vitro Antioxidant Activity Evaluation of 3-Carboxycoumarin Derivatives and QSAR Study of Their DPPH• Radical Scavenging Activity

Molecules ◽  
2012 ◽  
Vol 17 (12) ◽  
pp. 14882-14898 ◽  
Author(s):  
Francisco Martínez-Martínez ◽  
Rodrigo Razo-Hernández ◽  
Ana Peraza-Campos ◽  
Manuel Villanueva-García ◽  
Maria Sumaya-Martínez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Qsar Study ◽  
Dpph Radical Scavenging ◽  
In Vitro Antioxidant Activity

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY OF A MEDICINAL HERB, WEDELIA CHINENSIS (OSBECK) MERRILL.

2018 ◽  
Vol 11 (10) ◽  
pp. 433
Author(s):  
Rehana Banu H ◽  
Nagarajan N
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Total Antioxidant ◽  
Dpph Radical Scavenging ◽  
In Vitro Antioxidant Activity

Objective: The objective is to evaluate the antioxidant activity of the methanolic leaf extract of Wedelia chinensis.Methods: In vitro antioxidant activity was evaluated by studying 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, superoxide radical scavenging activity, ferric reducing ability of plasma (FRAP) scavenging activity, hydroxyl radical scavenging activity, metal chelating activity, and total antioxidant activity. Ascorbic acid, BHT, quercetin, and EDTA were used as standards for the experiments.Results: Inhibition concentration50 values observed for DPPH radical scavenging, superoxide radical scavenging, and hydroxyl radical scavenging activities were determined to be 62.5, 769.23, and 617.28 μg/ml, respectively and that of FRAP scavenging, metal chelating, and total antioxidant activities were found to be 343 μmol Fe (II)/g, 79.56 mg EDTA/g extract, and 398.17 mg ascorbic acid eq/g extract, respectively.Conclusion: The results clearly indicate that methanolic leaf extract of the study species W. chinensis is effective in scavenging free radicals and has the potential to be a powerful antioxidant.

scholarly journals Antioxidant Activity Profiling of Acetonic Extract of Jamun (Syzygium cumini L.) Seeds in Different In-Vitro Models

2020 ◽  
Vol 12 (1) ◽  
pp. 3-8
Author(s):  
Neha Yadav ◽  
Ajay Pal ◽  
Sonam Sihag ◽  
Nagesh C.R
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Metal Chelation ◽  
Syzygium Cumini

Background: Syzygium cumini L., commonly known as Jamun, black-plum, and Indian blackberry, is one of the most widely distributed trees in India with booming medical benefits and possesses antioxidant, anticancer and anti-diabetic properties. It belongs to the family Myrtaceae. Despite countless phytochemicals, seeds are not consumed and are the waste part of Jamun fruit. Objective: The objective of this study was to evaluate the antioxidant capacity of phenolics from Jamun seeds against a bundle of oxidant moieties. Methods: The 50% acetone extract of Jamun seeds was investigated for in-vitro antioxidant profiling. Assays include free radical scavenging activity, metal chelation activity, hydroxyl radical scavenging activity, hydrogen peroxide scavenging activity, total antioxidant activity, total reducing power, nitric oxide scavenging activity, and lipid peroxidation inhibition activity. Results: The extract depicted maximum DPPH radical scavenging activity followed by ABTS radical scavenging activity. Hefty metal chelation and nitric oxide scavenging activity were recorded while lipid peroxidation, H2O2, and OH- scavenging activity was intermediate. Conclusion: Jamun seed showed ample antioxidant activity and certifies that it is the right candidate for exploitation as a source of natural antioxidants to counteract autoxidation-induced pathologies or diseases.

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