scholarly journals ANGIOTENSIN CONVERTING ENZYME INHIBITORY ACTIVITY OF MELINJO (GNETUM GNEMON L.) SEED EXTRACTS AND MOLECULAR DOCKING OF ITS STILBENE CONSTITUENTS

2017 ◽  
Vol 10 (3) ◽  
pp. 243
Author(s):  
Abdul Mun'im ◽  
Muhammad Ashar Munadhil ◽  
Nuraini Puspitasari ◽  
Azminah . ◽  
Arry Yanuar
Keyword(s):  
Molecular Docking ◽  
Angiotensin Converting Enzyme ◽  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Total Phenolic ◽  
Converting Enzyme ◽  
Gnetum Gnemon ◽  
Ace Inhibitory

ABSTRACTObjectives: To evaluate the angiotensin converting enzyme (ACE) inhibitory activity of melinjo (Gnetum gnemon) seed extract and to study moleculardocking of stilbene contained in melinjo seeds.Methods: Melinjo seed powders were extracted with n-hexane, dichloromethane, ethyl acetate, methanol, and water successively. The extracts wereevaluated ACE inhibitory activities using ACE kit-Wist and the phenolic content using Folin–Ciocalteu method. The extract demonstrated the highestACE inhibitory activity was subjected to liquid chromatography-mass spectrometry (LC-MS) to know its stilbene constituent. The stilbene constituentsin melinjo seed were performed molecular docking using AutoDock Vina, and ligand-receptor Interactions were processed using Ligand Scout.Results: The ethyl acetate extract demonstrated the highest ACE inhibition activity with inhibitory concentration 50% value of 9.77 × 10−8 μg/mLand the highest total phenolic content (575.9 mg gallic acid equivalent/g). Ultra-performance LC-MS analysis of ethyl acetate extract has detected theexistency of resveratrol, gnetin C, ε-viniferin, and gnemonoside A/B. These compounds displayed similar physiochemical properties to lisinopril (ACEinhibitor), as in silico molecular docking studies demonstrated that they fit into the lisinopril receptors.Conclusion: In vitro analysis ethyl acetate extract from melinjo seeds demonstrated the highest ACE inhibitory activity. Molecular docking analysisindicated that resveratrol dimers, gnetin C and gnemonoside A can be considered ACE inhibitor.Keywords: Angiotensin converting enzyme inhibitor, Gnetum gnemon, Melinjo, Total phenolic, Antihypertension, Molecular docking.

scholarly journals ANGIOTENSIN I-CONVERTING ENZYME INHIBITORY ACTIVITY, TOTAL PHENOLIC AND FLAVONOID CONTENT OF EXTRACT AND FRACTION OF JAM FRUIT LEAVES (MUNTINGIA CALABURA L.)

2017 ◽  
Vol 10 (17) ◽  
pp. 166
Author(s):  
Eka Lasboi ◽  
Rissyelly Rissyelly ◽  
Katrin Katrin
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Phenolic Content ◽  
Ethyl Acetate Fraction ◽  
Hypotensive Effect ◽  
Total Phenolic ◽  
Angiotensin I ◽  
Ace Inhibitory Activity ◽  
Angiotensin I Converting Enzyme ◽  
Ace Inhibitory

 Objective: Hypertension is one of the most common chronic diseases. Inhibitory activity of angiotensin I-converting enzyme (ACE) is effective on giving hypotensive effect. Jamfruit leaf (Muntingia calabura L.) was reported to have an excellent hypotensive effect. This research was aimed to test in the manner of in vitro the inhibitory activity of ACE.Methods: This research used ACE kit-WST, total phenolic content, and total flavonoid from jam fruit leaf ethanol extract, hexane, ethyl acetate, and butanol fraction.Results: The result showed that Jamfruit leaf extract had ACE inhibitory activity and the most active fraction was ethyl acetate fraction. Inhibitory concentration 50% value of the most active fraction, ethyl acetate fraction was 0.63 μg/mL. Ethyl acetate fraction also provides most flavonoid and phenolic content with a value of 10.91 mg/g extract quercetin equivalent and 74.90 mg/g extract gallic acid equivalent.Conclusions: Ethyl acetate fraction of jam fruit leaf had most flavonoid, phenolic compound, and ACE inhibitory activity.

scholarly journals Angiotensin Converting Enzyme (ACE)-Peptide Interactions: Inhibition Kinetics, In Silico Molecular Docking and Stability Study of Three Novel Peptides Generated from Palm Kernel Cake Proteins

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 569 ◽  
Author(s):  
Mohammad Zarei ◽  
Najib Abidin ◽  
Shehu Auwal ◽  
Shyan Chay ◽  
Zaibunnisa Abdul Haiyee ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Angiotensin Converting Enzyme ◽  
Inhibitory Activity ◽  
Palm Kernel ◽  
Stability Study ◽  
Converting Enzyme ◽  
Palm Kernel Cake ◽  
Inhibition Kinetics ◽  
Ace Inhibitory Activity ◽  
Ace Inhibitory

Three novel peptide sequences identified from palm kernel cake (PKC) generated protein hydrolysate including YLLLK, WAFS and GVQEGAGHYALL were used for stability study against angiotensin-converting enzyme (ACE), ACE-inhibition kinetics and molecular docking studies. Results showed that the peptides were degraded at different cleavage degrees of 94%, 67% and 97% for YLLLK, WAFS and GVQEGAGHYALL, respectively, after 3 h of incubation with ACE. YLLLK was found to be the least stable (decreased ACE-inhibitory activity) compared to WAFS and GVQEGAGHYALL (increased ACE-inhibitory activity). YLLLK showed the lowest Ki (1.51 mM) in inhibition kinetics study when compared to WAFS and GVQEGAGHYALL with Ki of 2 mM and 3.18 mM, respectively. In addition, ACE revealed the lowest K m app and V max app and higher catalytic efficiency (CE) in the presence of YLLLK at different concentrations, implying that the enzyme catalysis decreased and hence the inhibition mode increased. Furthermore, YLLLK showed the lowest docking score of −8.224 and seven interactions with tACE, while peptide GVQEGAGHYALL showed the higher docking score of −7.006 and five interactions with tACE.

scholarly journals Biscuits from Fermented Roasted Buckwheat Flour - Phenolics Profile and Bioaccessible Angiotensin Converting Enzyme Inhibitory Activity

2020 ◽  
Vol 24 (2) ◽  
pp. 205-214
Author(s):  
Małgorzata Wronkowska ◽  
Joanna Honke ◽  
Henryk Zieliński ◽  
Wiesław Wiczkowski
Keyword(s):  
Phenolic Compounds ◽  
Angiotensin Converting Enzyme ◽  
Inhibitory Activity ◽  
Total Phenolic ◽  
Converting Enzyme ◽  
Total Phenolic Compounds ◽  
Ace Inhibitory Activity ◽  
Before And After ◽  
Ace Inhibitory ◽  
Buckwheat Flour

AbstractThe bioaccessible angiotensin converting enzyme (ACE) inhibitory activity of biscuits formulated from roasted common buckwheat flour after fermentation by select bacteria was studied. The same content of total phenolic compounds was found in fermented flour and in biscuits obtained from them. Generally, fermentation of flour did not changes the ACE inhibitory activity, whereas baking process significantly increased the ACE inhibitory activity of examined products. The potential bioaccessible ACE inhibitory activity from biscuits was very high. Phenolic acids such as protocatechuic, vanillic and syringic acids as well as flavonoids: kaempferol and epicatechin in the digested buckwheat biscuits have the highest impact on ACE inhibitory activity. A high significant correlations were found between IC50 and total phenolic compounds of fermented flours, biscuits before and after digestion. The data obtained in this study closely associates phenolic compounds with ACE inhibitory activity.

scholarly journals Angiotensin-converting enzyme (ACE) inhibitory activity of Solanum torvum and isolation of a novel methyl salicylate glycoside

2014 ◽  
Vol 11 ◽  
pp. 557-562 ◽  
Author(s):  
Arunee Simaratanamongkol ◽  
Kaoru Umehara ◽  
Hiroki Niki ◽  
Hiroshi Noguchi ◽  
Pharkphoom Panichayupakaranant
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Inhibitory Activity ◽  
Methyl Salicylate ◽  
Converting Enzyme ◽  
Ace Inhibitory Activity ◽  
Solanum Torvum ◽  
Ace Inhibitory

ANTIOXIDANT ACTIVITIES OF EXTRACTS FROM THE LEAVES AND STEM BARKS OF Artocarpus scortechinii KING

2015 ◽  
Vol 77 (2) ◽  
Author(s):  
Shajarahtunnur Jamil ◽  
Norazah Basar ◽  
Norzafneza Mohd Arriffin
Keyword(s):  
Ethyl Acetate ◽  
Gallic Acid ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Calibration Graph ◽  
Total Phenolic ◽  
Scavenging Capacity

The antioxidant activities of extracts (n-hexane, dichloromethane, ethyl acetate and methanol) from the leaves and stem barks of Artocarpus scortechinii were evaluated using various biochemical assays. The quantification of the Total Antioxidant Capacity was measured using ferric reducing antioxidant potential (FRAP) and 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulphonic acid) (ABTS) assays. While, the qualitative of The Total Phenolic Content (TPC) was determined via standard gallic acid calibration graph which was expressed as mg gallic acid equivalent (GAE)/g of dry weight (dw) using Folin Ciocalteau’s reagent. Among all the extracts tested, the methanolic extract of the stem barks showed the highest phenolic content with TPC value of 136.84 mg GAE/g dry weight (dw). FRAP results were expressed as mM equivalent to FeSO4.7H2O by calculating from the standard FeSO4.7H2O calibration graph. The ethyl acetate extract of the stem barks showed the most significant reducing potential in the range between 0.27-2.47 mM FRAP. ABTS+˙ radical scavenging capacity showed that the ethyl acetate extract of the stem barks had the highest scavenging capacity at concentration 1.0 mM with percentage of 90.9%.

scholarly journals Antioxidant, Anti-Diabetic and Phytochemical Screening of Dried Ficus carica Linn Fruit Powder

2020 ◽  
Vol 2 (1) ◽  
pp. 50
Keyword(s):  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Dpph Assay ◽  
Ic50 Value ◽  
Different Types

Ficus carica L. or the common name is red figs that belong to the Moraceae family or the Mulberry family. Every part of the fruit or the tree has been able to treat different types of common diseases, for example, it is used as a natural laxative or a supplementary food for diabetes. Thus, this research uses four different solvents, ethyl acetate, ethanol, hexane, and water, to obtain the respective crude extracts in order to investigate the anti-diabetic properties by determining the inhibition of the activity of the diabetic enzymes, α-amylase, and α-glucosidase; and the DPPH assay determines the antioxidant activity while qualitative phytochemical screening was for phenols, alkaloids, tannins, saponins, and flavonoids; total quantitative phenolic and total flavonoid content was done. The phytochemical screening showed the presence of the compounds tested in different types of crude extracts. For the total phenolic content, ethyl acetate extract exhibits the highest content. In contrast, hexane extract shows the highest total flavonoid content. For the DPPH assay, ethyl acetate extract has the highest scavenging activity at 13.351 µg/mL with corresponding with the data of total phenolic content. For the α-glucosidase inhibitory activity, water extract has the lowest IC50 value among the four extracts but higher value than the standard. For α-amylase inhibitory activity, only ethanol extract showed the IC50 value, but it was a high value. In conclusion, there is potential for figs to be a natural source of medicine, and the extracts tested can be used for future studies.

scholarly journals The Potential Angiotensin-Converting Enzyme Inhibitory Activity of Oleanolic Acid in the Hydroethanolic Extract of Crataegus monogyna Jacq

2006 ◽  
Vol 1 (5) ◽  
pp. 1934578X0600100 ◽  
Author(s):  
Everaldo Attard ◽  
Henrietta Attard
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Oleanolic Acid ◽  
Inhibitory Activity ◽  
Acid Group ◽  
Converting Enzyme ◽  
Ace Inhibitory Activity ◽  
Crataegus Monogyna ◽  
Triterpenic Acids ◽  
Hydroethanolic Extract ◽  
Ace Inhibitory

The hydroethanolic extract of Crataegus monogyna was studied for its chemical constitution and angiotensin-converting enzyme (ACE) inhibitory activity. The extract contained triterpenic acids, flavonoids and coumarins. The ACE inhibitory activity was studied using captropril, as a control drug, and oleanolic acid, as a constituent of the hydroethanolic extract and a member of the triterpenic acid group. The hydroethanolic extract and oleanolic acid showed higher IC50 values (335.00 μg/mL and 3.61 μM, respectively) in comparison to captopril (46.9 nM). However, these results indicate the anti-ACE activity of oleanolic acid and the triterpenic acids, which has not been demonstrated earlier for hawthorn extracts. In previous studies, the ACE inhibitory activity of C. monogyna extracts was always attributed to flavonoids and proanthocyanidins.

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