scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROSCOPY ANALYSIS OF ROOT OF AN ECONOMICALLY IMPORTANT PLANT, CENCHRUS CILIARIS L. FROM THAR DESERT, RAJASTHAN (INDIA)

Author(s):  
Sunita Arora ◽  
Ganesh Kumar ◽  
Sonam Meena

Objective: The study was carried out with an objective to characterize the possible bioactive phytochemical constituents from the root of Cenchrus ciliaris L. using various solvents of different polarities, i.e., methanol, ethyl acetate, and hexane by gas chromatography-mass spectroscopy (GC-MS) analysis.Methods: Plant material was collected from harsh, xeric conditions of “Thar” during August to October. The shade-dried plant root powder was extracted with solvents using Soxhlet extractor. The phytochemical compounds were investigated using PerkinElmer GC-MS, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology and Willey 8 library.Results: Maximum % area is found for stigmasta-5,22-dien-3-ol and present in maximum amount (12.68%) with reaction time (RT)=36.461 minutes in the methanolic extract. 1,2,3-propanetriol, 1-acetate is present in maximum amount (13.15%) with RT=6.582 minutes in the ethyl acetate extract. Tetracontane is present in maximum amount (16.70%) with RT=18.744 minutes in the hexane extract of root of C. ciliaris L.Conclusions: Green plants synthesize and preserve a variety of biochemical compounds. Plant secondary metabolites are commercially important and are used by pharmaceutical industry as well as the traditional practitioners. The GC-MS study helps to predict the formula and structure of phytoconstituents that can be used for drug design, and further investigation may lead to the development of various drug formulations. 

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Khurriyatul Khair ◽  
Yayuk Andayani ◽  
Aliefman Hakim

This research was aimed to identify some classes of secondary metabolite compound on the fractionation results of green bean extract (P. vulgaris L.) using Gas Chromatography-Mass Spectroscopy (GC-MS) method. Green bean was extracted by methanol solvent. Fractionation of methanol extract used Vacuum Liquid Chromatography (VLC) with some variation of eluents such as n-hexane 100%; n-hexane: ethyl acetate (9 : 1 to 1 : 9); and ethyl acetate 100%, produced 11 major fractions.  Based on Thin Layer Chromatography (TLC) analysis using DCM: MeOH (9.5: 0.5) as mobile phases, these 11 major fractions were classified based on their polarity such as non-polar, semi-polar and polar fraction. The results of identification by GC-MS spectrometer showed the presence of secondary metabolites such as monoterpenes in non-polar fraction; in semi-polar fraction was found terpenoids and steroids; in polar fraction was found monoterpenes, phenolic and steroid as the lowest percent area, that is less than 2%. Keyword: P.vulgaris L extract, fractionation, VLC, GC-MS


2009 ◽  
Vol 1 (2) ◽  
pp. 387-392 ◽  
Author(s):  
M. N. I. Bhuiyan ◽  
J. Begum ◽  
P. K. Sardar ◽  
M. S. Rahman

The chemical constituents of leaf and peel essential oil of Citrus medica L. were analysed by gas chromatography mass spectroscopy (GC-MS). Nineteen components accounting for 99.9% of the oil were identified in leaf oil. The major constituents are erucylamide (28.43%), limonene (18.36%) and citral (12.95%). The peel oil contains forty three components accounting for 99.8% of the total oil and the major components are isolimonene (39.37%), citral (23.12%) and limonene (21.78%). Keywords: Citrus medica; Essential oils; GC-MS; Erucylamide; Isolimonene. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.DOI: 10.3329/jsr.v1i2.1760   


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