scholarly journals IN VITRO ANTIOXIDANT AND ANTIULCEROGENIC ACTIVITY OF CAYRATIA PEDATA VAR. GLABRA AGAINST EXPERIMENTALLY INDUCED GASTRIC LESIONS IN WISTAR STRAIN ALBINO RATS.

2018 ◽  
Vol 11 (1) ◽  
pp. 105
Author(s):  
Kalaichelvi K ◽  
Sharmila S ◽  
Dhivya Sm
Keyword(s):  
Free Radical ◽  
Plant Extracts ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Gastric Ulcers ◽  
Total Phenolic ◽  
Albino Rats ◽  
Antiulcer Activity ◽  
Dependent Manner

Objective: To quantify the phytochemical constituents, scavenging different free radicals and assess the antiulcer property of aerial plant extracts of Cayratia pedata var. glabra.Methods: Quantitative analysis was performed to confirm and quantify the presence of phenolics, tannin content and total flavonoids in the aerial plant extracts of the study plant. Reducing power assay, 2, 2- diphenyl-1-picryl-hydrazyl (ABTS), ABTS•+ and metal chelating activity were used in this study. To evaluate the anti-ulcerogenic activity of ethanolic extract of C. pedata var. glabra, against indomethacin-induced gastric ulcers in rats.Results: The results of quantification of phytoconstituents showed that the total phenolic and total flavonoid content were maximum in the ethanolic extract. The results of the antioxidant property of C. pedata var. glabra showed that all the extracts possess a different range of free radical scavenging activities, which is in dose-dependent manner. The ethanolic extract of the study plant produced gastroprotective effect in indomethacin-induced rats showed the decreased level of ulcer index, gastric volume, free acidity, total acidity, total protein and increased level of percentage of protection, and pH were calculated from the effective concentrations of plant sample (400 mg/kg) compared with Omeprazole (10 mg/kg).Conclusions: It is concluded that, the C. pedata var. glabra contains strong secondary metabolites; natural source of antioxidants and different extracts of this plant possesses good free radical scavenging activity. This present study indicates that ethanolic extract of C. pedata var. glabra has potential antiulcer activity in this tested model. In future, it can be used as a bioactive source of natural antioxidants and are potential natural resources for pharmacology of functional foods.

scholarly journals IN VITRO ANTIOXIDANT AND ANTIULCEROGENIC ACTIVITY OF CAYRATIA PEDATA VAR. GLABRA AGAINST EXPERIMENTALLY INDUCED GASTRIC LESIONS IN WISTAR STRAIN ALBINO RATS.

2018 ◽  
Vol 11 (1) ◽  
pp. 105
Author(s):  
Kalaichelvi K ◽  
Sharmila S ◽  
Dhivya Sm
Keyword(s):  
Free Radical ◽  
Plant Extracts ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Gastric Ulcers ◽  
Total Phenolic ◽  
Albino Rats ◽  
Antiulcer Activity ◽  
Dependent Manner

Objective: To quantify the phytochemical constituents, scavenging different free radicals and assess the antiulcer property of aerial plant extracts of Cayratia pedata var. glabra.Methods: Quantitative analysis was performed to confirm and quantify the presence of phenolics, tannin content and total flavonoids in the aerial plant extracts of the study plant. Reducing power assay, 2, 2- diphenyl-1-picryl-hydrazyl (ABTS), ABTS•+ and metal chelating activity were used in this study. To evaluate the anti-ulcerogenic activity of ethanolic extract of C. pedata var. glabra, against indomethacin-induced gastric ulcers in rats.Results: The results of quantification of phytoconstituents showed that the total phenolic and total flavonoid content were maximum in the ethanolic extract. The results of the antioxidant property of C. pedata var. glabra showed that all the extracts possess a different range of free radical scavenging activities, which is in dose-dependent manner. The ethanolic extract of the study plant produced gastroprotective effect in indomethacin-induced rats showed the decreased level of ulcer index, gastric volume, free acidity, total acidity, total protein and increased level of percentage of protection, and pH were calculated from the effective concentrations of plant sample (400 mg/kg) compared with Omeprazole (10 mg/kg).Conclusions: It is concluded that, the C. pedata var. glabra contains strong secondary metabolites; natural source of antioxidants and different extracts of this plant possesses good free radical scavenging activity. This present study indicates that ethanolic extract of C. pedata var. glabra has potential antiulcer activity in this tested model. In future, it can be used as a bioactive source of natural antioxidants and are potential natural resources for pharmacology of functional foods.

Evaluation of Antioxidant Activities of Alpinia galanga (L.) Willd

2018 ◽  
Vol 15 (4) ◽  
pp. 899-908
Author(s):  
Khoirom Ratipiyari Devi ◽  
Paonam Priyobrata Singh ◽  
Moirangthem Medhapati Devi ◽  
Gurumayum Jitendra Sharma
Keyword(s):  
Free Radical ◽  
Plant Extracts ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Alpinia Galanga ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Promising Source

Present research was designed to evaluate the free radical scavenging capacities and antioxidant activities of rhizome extracts of Alpinia galanga prepared in different solvent systems (60% aqueous methanol, 60% aqueous ethanol and distilled water) using different in vitro chemical assays. Antioxidant components such as total phenolic content (TPC), total flavonoid content (TFC) and ascorbic acid contents of the ginger species were screened. Antioxidant assays employed included sulphur free radical reactivity assay, ferric ion reducing power assay, DPPH free radical scavenging capacity assay, hydroxyl radical scavenging assay, nitric oxide scavenging activity assay and hydrogen peroxide scavenging assay. The obtained data reveal that the plant extracts contained significant amount of the observed antioxidant components and also exhibited significant free radical scavenging capacities. Methanol (60%) extract exhibited highest antioxidant activity than other solvents. The polyphenolic constituents of the plant extracts appear to be largely responsible for the radical scavenging capacity. The plant extracts act as promising source of antioxidants, and may be useful for development of nutraceuticals and pharmaceutical drugs.

scholarly journals Antioxidant, Antimicrobial, Anti-diarrheal and Analgesic Activities of Diospyros malabarica (Desr.) Kostel

2019 ◽  
Vol 22 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Md Moniruzzaman ◽  
Md Ruhul Kuddus ◽  
AM Sarwaruddin Chowdhury ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Tail Flick ◽  
Dependent Manner ◽  
Analgesic Activities

The present study was designed to evaluate the antioxidant, antimicrobial, antidiarrheal and analgesic activities of methanol extract of Diospyros malabarica (Desr.) Kostel buds growing in Bangladesh. The total phenolic content of methanol extract of D. malabarica and its Kupchan fractions was determined and expressed in gallic acid equivalent (GAE). In the DPPH free radical scavenging assay, the aqueous soluble fraction of the methanol extract of D. malabarica revealed maximum free radical scavenging activity having IC50 value of 6.17 μg/ml. In antimicrobial assay, all the test samples displayed prominent antimicrobial activity against the test organisms under in vitro conditions. Among these, the carbon tetrachloride soluble fraction was found to exhibit the highest activity against Bacillus cereus, Salmonella Typhi, Salmonella Paratyphi, and Candida albicans with the zone of inhibition as 32, 30, 28 and 30 mm, respectively. In evaluation of antidiarrheal activity, the D. malabarica extracts showed significant anti-diarrheal potential in a dose dependent manner. During the evaluation of analgesic activity by radiant heat tail-flick method, the plant extract at 400 mg/kg b.w. exhibited highest elongation (373.04%) as compared to morphine (472.48%). On the other hand, in acetic acid-induced writhing test, the extract at 200- and 400-mg/kg b.w. showed 61.11 and 66.67% inhibition of writhing in mice model, respectively as compared to 68.06% inhibition produced by the standard diclofenac-Na. The findings of this study justify some of the traditional uses of D. malabarica and reveal the bioactivity of the plants. Further studies are required to isolate and identify the bioactive compounds. Bangladesh Pharmaceutical Journal 22(1): 27-33, 2019

Assessment of free radical scavenging activity and polyphenolic content in leaves of Neurocalyx calycinus (R. Br. ex Benn.) Rob. - a medicinal plant used by Cholanaickan tribe

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Ajayakumar Lalitha Aneeshkumar
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Dried Extract

Neurocalyx calycinus (R. Br. ex Benn.) Rob. is an endemic species of the family Rubiaceae, found in Western Ghats of India and Sri Lanka. The leaf paste of N. calycinus has been used by Cholanaickan tribe as medicine to heal wounds, burns, inflammation and pain. Many of the human diseases are manifested due to the accumulation of free radicals. Plant derived antioxidants have the power to scavenge these free radicals without any internal complications. Aqueous, n-hexane, chloroform, ethyl acetate and ethanol extracts of the leaves of N. calycinus were selected for qualitative phytochemical screening. DPPH, nitric oxide, superoxide and hydroxyl radical scavenging assays were carried out. Total phenolic, flavonoid and antioxidant content of all the extract was measured. Phytochemical screening showed that alkaloids, steroids, phenolic compounds, glycosides and saponins were found in the ethanolic extract. Ethanol extract showed better antioxidant activity than the other solvent extracts. The reducing power of all the extracts was increased dose dependently. Highest amount of phenolic (56.27 mg of gallic acid equivalent /gm of dried extract), flavonoid (36.92 mg of quercetin equivalent/gm of dried extract) and antioxidant (85.68 mg of ascorbic acid equivalent/gm of dried extract) content was found in the ethanolic extract. A positive correlation (R2) was found between the total phenolic content and free radical scavenging activity of the leaf extract of N. calycinus. The results confirmed that the phenolic compounds in the ethanolic extract of the leaves of N. calycinus are potent source of natural antioxidants.

scholarly journals Hepatoprotective potential of Indigofera tirunelvelica Sanjappa: in vitro and in vivo studies on CCl4 induced wistar albino rats

2020 ◽  
Vol 11 (4) ◽  
pp. 6404-6410
Author(s):  
Subburayalu S ◽  
Asha KRT ◽  
Deepa Somanath ◽  
Palavesam A
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Albino Rats ◽  
Whole Plant ◽  
Wistar Albino Rats

The hepatoprotective efficiency of Indigofera tirunelvelica Sanjappa whole plant against CCl4 induced hepatotoxicity was examined. Rat hepatocyte monolayer culture and wistar albino rats were exercised as in vitro and in vivo screening models of protective agent for liver. In in vitro analyses, the whole plant ethanolic extract of Indigofera tirunelvelica Sanjappa were inspected. Silymarin was chosen as a standard treatement drug. In vitro, free radical scavenging property was also evaluated. In animal studies, hepatotoxicity was produced in Wistar albino rats by dispensing CCl4. The degree of hepatotoxicity was examined by determining the ranges of serum enzyme. The antioxidant parameters such as superoxide dismutase, catalase, reduced glutathione, and malondialdehyde of the hepatocytes were also evaluated. In in vitro studies, ethanol extract of I. tirunelvelica whole plant was identified to be the most active than other assessed extracts. Besides, whole plant ethanol extract of I. tirunelvelica was noticed to be rich in phenolic and flavonoids. It exhibited expressive free radical scavenging property versus diphenylpicryl hydrazyl (DPPH) and superoxide ion radicals. In the animals studies, whole plant ethanolic extract of I. tirunelvelica at a ranges of doses (100, 200 and 400 mg/kg body weight) revealed considerable amount of protection against CCl4 induced hepatotoxicity as evident by the protection of CCl4 induced changes biochemical parameters. The results of the present study suggested that the significant hepatoprotective property of whole plant ethanol extract of I. tirunelvelica against CCl4 induced hepatotoxicity and intimates its use as a potential medicinal drug for liver diseases.

EVALUATION OF ANTIOXIDANT POTENTIAL OF CARALLUMA ATTENUATA

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (04) ◽  
pp. 26-33
Author(s):  
V. Sama ◽  
◽  
B. Rajesh ◽  
A. Krishnaiah ◽  
A Ravikiran ◽  
...  
Keyword(s):  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antioxidant Property ◽  
In Vitro Models ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Nitric Oxide Radical

Antioxidant activity of ethanolic extract of Caralluma attenuata was evaluated for its free radical scavenging property in different in vitro models. The extract showed good dose dependent free radical scavenging property in all models except in inhibition of nitric oxide radical and lipid peroxidation. IC50 values are found to be 10, 14, 110 µg/mL in DPPH, super oxide and hydroxyl radical scavenging assays, respectively. Measurement of total phenolic compounds by Folin Ciocalteau phenol reagent indicated that 1 g of extract contain 450 mg of GAE/g. The results indicate that the antioxidant property of extract may be due to high content of phenolic compounds.

scholarly journals Chemical Analysis, Toxicity Study, and Free-Radical Scavenging and Iron-Binding Assays Involving Coffee (Coffea arabica) Extracts

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4169
Author(s):  
Nuntouchaporn Hutachok ◽  
Pimpisid Koonyosying ◽  
Tanachai Pankasemsuk ◽  
Pongsak Angkasith ◽  
Chaiwat Chumpun ◽  
...  
Keyword(s):  
Free Radical ◽  
Mononuclear Cells ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Chemical Compositions ◽  
Coffee Bean ◽  
Dependent Manner ◽  
Roasted Coffee ◽  
Instant Coffee ◽  
Radical Scavenging Properties

We aimed to analyze the chemical compositions in Arabica coffee bean extracts, assess the relevant antioxidant and iron-chelating activities in coffee extracts and instant coffee, and evaluate the toxicity in roasted coffee. Coffee beans were extracted using boiling, drip-filtered and espresso brewing methods. Certain phenolics were investigated including trigonelline, caffeic acid and their derivatives, gallic acid, epicatechin, chlorogenic acid (CGA) and their derivatives, p-coumaroylquinic acid, p-coumaroyl glucoside, the rutin and syringic acid that exist in green and roasted coffee extracts, along with dimethoxycinnamic acid, caffeoylarbutin and cymaroside that may be present in green coffee bean extracts. Different phytochemicals were also detected in all of the coffee extracts. Roasted coffee extracts and instant coffees exhibited free-radical scavenging properties in a dose-dependent manner, for which drip coffee was observed to be the most effective (p < 0.05). All coffee extracts, instant coffee varieties and CGA could effectively bind ferric ion in a concentration-dependent manner resulting in an iron-bound complex. Roasted coffee extracts were neither toxic to normal mononuclear cells nor breast cancer cells. The findings indicate that phenolics, particularly CGA, could effectively contribute to the iron-chelating and free-radical scavenging properties observed in coffee brews. Thus, coffee may possess high pharmacological value and could be utilized as a health beverage.

scholarly journals Antioxidant Rich Extracts of Terminalia ferdinandiana Inhibit the Growth of Foodborne Bacteria

Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 281 ◽  
Author(s):  
Saleha Akter ◽  
Michael E. Netzel ◽  
Ujang Tinggi ◽  
Simone A. Osborne ◽  
Mary T. Fletcher ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Food Preservation ◽  
Total Phenolic ◽  
Foodborne Bacteria ◽  
Terminalia Ferdinandiana

Terminalia ferdinandiana (Kakadu plum) is a native Australian plant containing phytochemicals with antioxidant capacity. In the search for alternatives to synthetic preservatives, antioxidants from plants and herbs are increasingly being investigated for the preservation of food. In this study, extracts were prepared from Terminalia ferdinandiana fruit, leaves, seedcoats, and bark using different solvents. Hydrolysable and condensed tannin contents in the extracts were determined, as well as antioxidant capacity, by measuring the total phenolic content (TPC) and free radical scavenging activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. Total phenolic content was higher in the fruits and barks with methanol extracts, containing the highest TPC, hydrolysable tannins, and DPPH-free radical scavenging capacity (12.2 ± 2.8 g/100 g dry weight (DW), 55 ± 2 mg/100 g DW, and 93% respectively). Saponins and condensed tannins were highest in bark extracts (7.0 ± 0.2 and 6.5 ± 0.7 g/100 g DW). The antimicrobial activity of extracts from fruit and leaves showed larger zones of inhibition, compared to seedcoats and barks, against the foodborne bacteria Listeria monocytogenes, Bacillus cereus, Methicillin resistant Staphylococcus aureus, and clinical isolates of Pseudomonas aeruginosa. The minimum inhibitory concentration and minimum bactericidal concentration in response to the different extracts ranged from 1.0 to 3.0 mg/mL. Scanning electron microscopy images of the treated bacteria showed morphological changes, leading to cell death. These results suggest that antioxidant rich extracts of Terminalia ferdinandiana fruits and leaves have potential applications as natural antimicrobials in food preservation.

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