scholarly journals ISOLATION OF UROPATHOGENS AND THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN AT A TERTIARY CARE HOSPITAL IN NORTHERN INDIA

Author(s):  
HARIT KUMAR ◽  
VARSHA A SINGH ◽  
SHAVI NAGPAL ◽  
DIPANKAR BISWAS

Objective: The objective of the study was to know the isolation rate and antibiotic susceptibility pattern of pathogens causing urinary tract infection (UTI). Methods: A total of 500 urine samples of clinically suspected UTI were collected from January 2019 to June 2019. The samples were inoculated on Cystine Lactose Electrolyte-Deficient agar and incubated at 37°C for 24 h. Results: In our study, 303 (60.6%) samples showed growth of isolates. Among 303 isolates, 31 were Candida spp. which was not included in the study. Out of 272 isolates, Escherichia coli was 31.68%, followed by Klebsiella spp. (27.72%), Enterococcus faecalis (22.44%), Citrobacter spp. (3.96%), Staphylococcus aureus (2.64%), and Pseudomonas spp. (1.32%). For Gram-negative and Gram-positive isolates, the most effective antibiotic was nitrofurantoin. Conclusion: The study shows E. coli as the predominant pathogen that causes UTI. Nitrofurantoin and fosfomycin showed very high susceptibility to uropathogens and can be used to treat primary or uncomplicated UTI.

Author(s):  
Vijayashree V. ◽  
Saikeerthana D. ◽  
Prabha P.

Background: The genus Klebsiella of Enterobacteriaceae family is ubiquitous in nature. They cause many nosocomial infections like pneumonia, urinary tract infections, wound infections, bacteremia and septicemia. Multidrug resistance is seen in Klebsiella which serves as the most common cause of increased morbidity and mortality. This study reveals the prevalence and antibiotic sensitivity pattern of Klebsiella species from various clinical samples. The primary objectives are as follows: To isolate and characterize Klebsiella species from various clinical samples. To study the antibiotic susceptibility pattern of Klebsiella isolates.Methods: This prospective study was conducted in our tertiary care hospital during the period from August 2019 to October 2019. A total of 3521 samples were tested during this period. The samples include blood, sputum, urine, and pus.Results: Out of the total samples tested,1106 samples were showing the growth of the organisms. Among this,351 were identified as Klebsiella species and the highest rate of isolation of Klebsiella species is from the sputum sample and also the same was reported high in medical wards. The study also shows that the isolation of Klebsiella species shows male preponderance when compared to females. The antibiotic sensitivity pattern was done by Kirby-Bauer's disc diffusion method and the sensitivity was noted to be higher to amikacin and ciprofloxacin.Conclusions: Thus, this study shows the prevalence rate of Klebsiella species and sensitivity pattern of Klebsiella, which may help select appropriate antibiotics and prevent overuse and misuse of antibiotics.


2015 ◽  
Vol 13 (1) ◽  
pp. 71-78 ◽  
Author(s):  
UT Tasnim ◽  
MT Islam

Milk is such a food which can meet almost all nutritional needs of human lives. Raw or unprocessed milk supports the growth of wide variety of microorganisms. The major interests of this study were examining the microbial quality of raw milk collected from different locations of Jessore city in Bangladesh and determining antibiotic susceptibility pattern of some isolated bacteria. To do so, 12 raw milk samples were collected from different areas of Jessore city. Microbial analysis comprised of enumeration of TVC (total viable count), TCC (total coliform count) and TSC (total staphylococcal count). The highest TVC, TCC and TSC were 1.95x109 CFU/ml, 2.5x107 CFU/ml and 1.02x107 CFU/ml respectively. Prevalent bacterial populations were Klebsiella spp., Enterobacter spp., Shigella spp. Staphylococcus spp., Escherichia coli and Citrobacter spp. In order to observe the antibiotic susceptibility pattern, the antibiotic sensitivity test was performed for some randomly selected isolates of E. coli and Klebsiella spp. More than 90% isolates of Klebsiella spp. were found to be resistant against Erythromycin whereas more than 90% isolates were sensitive against Imipenem. On the other hand, 100% E. coli isolates were observed as resistant against Erythromycin and in case of Trimethopreme 100% isolates were sensitive. Multidrug resistance pattern was also found. These results suggest the necessity of hygienic practices during handling, processing and post-processing of raw milk to improve the microbiological quality and safety of raw milk.DOI: http://dx.doi.org/10.3329/bjvm.v13i1.23723Bangl. J. Vet. Med. (2015). 13 (1): 71-78


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