scholarly journals Effects of L-lysine on cytogenetical and physiological parameters in Allium cepa L. under salt stress

2019 ◽  
Vol 48 (3) ◽  
pp. 625-632
Author(s):  
Dilek Çavuşoğlu
Keyword(s):  
Seed Germination ◽  
Allium Cepa ◽  
Seedling Growth ◽  
Chromosomal Abnormalities ◽  
Inhibitory Effect ◽  
Nacl Stress ◽  
Physiological Parameters ◽  
Root Tip ◽  
Allium Cepa L

The role of L-lysine (Lys) on some cytogenetic and physiological parameters in Allium cepa L. seeds exposed to salt was evaluated. NaCl stress on the other hand showed a significantly inhibitory effect on the seedling growth and seed germination of Allium cepa. Besides, it significantly reduced the mitotic index in the root tip meristems of seeds and increased micronuclei which are the most effective and simplest indicator of cytological damage and chromosomal abnormalities. However, the effects of salinity on chromosomal aberrations, seedling growth, seed germination and mitotic activity have decreased significantly with Lys application.

scholarly journals Effects of sodium hypochlorite on some physiological and cytogenetical parameters in Allium cepa L. exposed to salt stress

2019 ◽  
Vol 48 (2) ◽  
pp. 223-229 ◽  
Author(s):  
Kürşat Çavuşoğlu ◽  
Fadime Doğu ◽  
Dilek Çavuşoğlu
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Mitotic Activity ◽  
Allium Cepa ◽  
Sodium Hypochlorite ◽  
Seedling Growth ◽  
Chromosomal Aberrations ◽  
Root Tip ◽  
Fresh Weight ◽  
Allium Cepa L

The effects of sodium hypochlorite (NaClO) on the seed germination, seedling growth (radicle length, radicle number and fresh weight), mitotic activity and chromosomal aberrations of Allium cepa L. germinated under salt stress were studied. Salt stress considerably inhibited the seed germination and seedling growth of A. cepa. Furthermore, it markedly reduced the mitotic index in root tip meristems of the seeds and increased the number of chromosomal aberrations. Whereas, the detrimental effects of salt on the seed germination, seedling growth, mitotic activitiy and chromosomal aberrations were dramatically alleviated in varying degrees by NaClO application.

scholarly journals Zinc Oxide and Zinc Oxide Nanoparticles Impact on In Vitro Germination and Seedling Growth in Allium cepa L.

Materials ◽  
2020 ◽  
Vol 13 (12) ◽  
pp. 2784 ◽  
Author(s):  
Alicja Tymoszuk ◽  
Jacek Wojnarowicz
Keyword(s):  
Zinc Oxide ◽  
Seed Germination ◽  
Allium Cepa ◽  
Seedling Growth ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Allium Cepa L ◽  
Germinating Seeds

Zinc oxide nanoparticles (ZnO NPs) are ones of the most commonly manufactured nanomaterials worldwide. They can be used as a zinc fertilizer in agriculture to enhance yielding and to control the occurrence of diseases thanks to its broad antifungal and antibacterial action. The aim of this study was to investigate and compare the effects of ZnO submicron particles (ZnO SMPs) and ZnO NPs on the process of in vitro seed germination and seedling growth in onion (Allium cepa L. ‘Sochaczewska’), and to indicate the potential use of these compounds in onion production. In the experiment, disinfected seeds were inoculated on the modified Murashige and Skoog (MS) medium and poured with ZnO SMP or ZnO NP water suspension, at the concentrations of 50, 100, 200, 400, 800, 1600, and 3200 mg∙L−1. During three successive weeks, the germinating seeds were counted. Germination started most often on the second or third day of in vitro culture. The highest share of germination was recorded for seeds treated with 800 mg∙L−1 ZnO SMPs and ZnO NPs (52% and 56%, respectively). After the application of ZnO SMPs and ZnO NPs at the highest tested concentration (3200 mg∙L−1), the share of germinating seeds was only 19% and 11%, respectively. Interestingly, seedlings obtained from control seeds and seeds treated with ZnO SMPs and ZnO NPs did not differ statistically in terms of length, fresh weight, and dry weight of leaves, and roots. Both ZnO SMPs and ZnO NPs, in the concentration range from 50 to 1600 mg∙L−1, can be used to stimulate the germination process of onion seeds, without negative effects on the further growth and development of seedlings. There were no differences found between the action of ZnO NPs and ZnO SMPs, which suggested that the most important factor influencing seed germination was in fact the concentration of zinc ions, not the particle size.

scholarly journals Cytological Effects of Leaf Extract of Artemisia vulgaris L. on Meristematic Cells of Allium cepa L.

Our Nature ◽  
2013 ◽  
Vol 10 (1) ◽  
pp. 242-248 ◽  
Author(s):  
P.D. Rai ◽  
N. Paudel ◽  
S.R. Shakya
Keyword(s):  
Allium Cepa ◽  
Leaf Extract ◽  
Chromosomal Abnormalities ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Artemisia Vulgaris ◽  
Active Components ◽  
Duration Of Treatment ◽  
Cytological Effect ◽  
Allium Cepa L

Artemisia vulgaris L., a perennial aromatic shrub with bitter taste, is considered as a medicinal plant and water extract of it consists of active components like psilostachyin A, psilostachy C, Exiguaflavanone A, Maackiain, fernenol with both anti-bacterial and medicinal value. It has been used to perserve crops and stored grains both in liquid and powder by farmers. The cytological effect was observed in 5, 7.5, 10 and 20% concentrations of Leaf Extract of Artemisia vulgaris (LEA) on Allium cepa L. in five different time durations. The present study showed the correlation between concentration of LEA and duration of treatment in controlled state. Higher concentrations of LEA as well as longer duration of treatment generate the inhibitory effect on the division of cells. Lower concentration of LEA showed less effect on Mitotic Index value (MI) in comparison with control value, which is attributed to delay in metabolic activities. It also showed the change in phase indices and induced chromosomal abnormalities among the phases.DOI: http://dx.doi.org/10.3126/on.v10i1.7787

scholarly journals Boron induces seed germination and seedling growth of Hordeum vulgare L. under Nacl stress

2018 ◽  
Vol 8 ◽  
pp. 1224-1234
Author(s):  
Saud A. Alamri ◽  
Manzer H Siddiqui ◽  
Mutahhar Y. Al-Khaishani ◽  
Hayssam M. Ali
Keyword(s):  
Hordeum Vulgare ◽  
Seed Germination ◽  
Seedling Growth ◽  
Growth Traits ◽  
Nacl Stress ◽  
High Dose ◽  
Barley Plant ◽  
Hordeum Vulgare L ◽  
Viable Cells

Boron (B), an essential micronutrient, helps the plants to complete their life cycle successfully. Therefore, the present experiment was conducted to study (1) the role of B in seed germination and seedling growth, (2) the toxicity effect of B in seed germination and seedling growth and (3) the role of B in tolerance of barley (Hordeum vulgare L. var. ‘Bakore’) to NaCl stress. Under NaCl stress and non-stress conditions, application of high levels of B (100 µM) decreased parameters of germination (G%, VI, GI and MGT), growth (RL, SL, RFW, SFW, RDW and SDW), except the accumulation of Pro and MDA in barley seedlings. Also, a fluorescence study reveals that production of ROS (H2O2 and O2 •—) and non-viable cells increased in roots of barley seedlings treated with NaCl and high dose of B. An alteration in anatomical structure of barley seedlings was observed with the application of NaCl and high dose of B. However, a low concentration of B (50 µM) proved best and increased all germination and growth traits of barley seedlings by increasing further accumulation of Pro. Also, 50 µM of B significantly increased the biosynthesis of photosynthetic pigments (Chl a, b and total Chl) and deceased formation of ROS and viable cells in roots. Therefore, concluded that sufficient dose of B could be beneficial for barley plant in improving the tolerance to NaCl stress.

scholarly journals CYTO-DEPRESSIVE POTENTIAL OF GANODERMA LUCIDUM, PLEUROTUS OSTREATUS AND THEIR BINARY COMBINATIONS AGAINST MERISTEMATIC CELLS OF ALLIUM CEPA L.

2019 ◽  
pp. 320-324
Author(s):  
MOYEN UDDIN PK
Keyword(s):  
Allium Cepa ◽  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Root Tip ◽  
Pharmacological Properties ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Meristematic Cells ◽  
Allium Cepa L

Objective: The current study is designed to evaluate the cyto-depressive effects of Ganoderma lucidum (GL) and Pleurotus ostreatus (PO) and their binary amalgams (GL+PO) on meristematic cells of Allium cepa L. Methods: Experiments were assessed by utilizing A. cepa bioassay. Results: Onion knobs were exposed to 500 μg/mL, 1000 μg/mL, and 2000 μg/mL groupings of the concentrates for naturally visible and minute examination. The concentrates essentially repressed the root development of A. cepa L. contrasted with the control in a period of treatment and concentration-dependent manner (p<0.05). In addition, photomicrographs revealed chromosomal aberrations extending from c-mitosis, stickiness, and bridges in the root tip meristematic cells of A. cepa. All mushroom extracts demonstrated remarkable cyto-depressive impacts on meristematic cells of A. cepa L. with some changeability. Conclusion: In view of the results come out, GL and PO could be considered as potential sources of anticancer mixes. Notwithstanding, additionally thinks about are essential for disengagement and portrayal of the bioactive mixes and more examination on their pharmacological properties is required. It is first report in Bangladesh where we studied cyto-depressive role of edible mushrooms, Ganoderma lucidum (GL) and Pleurotus ostreatus (PO).

Effects of nitrogen on sweet sorghum seed germination, seedling growth and physiological traits under NaCl stress

2013 ◽  
Vol 20 (10) ◽  
pp. 1303-1309 ◽  
Author(s):  
Jing MU ◽  
Xiao-Jing LIU ◽  
Jin XU ◽  
Ren-Zhao MAO ◽  
Wei WEI ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Sweet Sorghum ◽  
Nacl Stress ◽  
Physiological Traits

Allelopathic Potency and an Active Substance from Hairy Vetch

2020 ◽  
Author(s):  
Weihong Sun ◽  
Guofeng Yang ◽  
Lili Cong ◽  
Juan Sun ◽  
Lichao Ma
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Root Length ◽  
Leaf Extract ◽  
Growth Inhibitor ◽  
Inhibitory Effect ◽  
Allelopathic Effect ◽  
Coumaric Acid ◽  
Hairy Vetch ◽  
Allelopathic Effects

Background: Plant allelopathy refers to the release of chemicals from plants or microorganisms into the environment, may have direct or indirect, beneficial or harmful effects on other plants or microorganisms. When plants grow in an unfavorable environment,more allelochemicals will be secreted and the expression of allelopathic effects will increase, giving plants a certain competitive advantage. Hairy vetch is one of the most promising allelopathic crops and the aqueous extract of hairy vetch has an inhibitory effect on the root length and seedling height of grass crops. The current study aimed to study the allelopathic effect of hairy vetch on alfalfa, and exploring an ecological method to remove the root system of alfalfa.Methods: In this experiment, the allelopathic effects of the seeds, stems and leaves, roots extracts and root exudates (0, 3, 6, 9 and 12 mg·mL-1) on the seed germination and seedling growth of alfalfa were evaluated. And the main allelopathic substances from the stem and leaf extract were isolated and identified using high performance liquid chromatography- mass spectrometry (HPLC-MS).Result: The results showed that all of the extracts can inhibit alfalfa seed germination and seedling growth and stem and leaf extract had the strongest inhibitory effect, especially for inhibiting the root growth. A main allelochemical substance, o-coumaric acid, was screened out and the root length of alfalfa was completely inhibited at 1.6 mg·mL-1 of o-coumaric acid. The findings of these experiments show hairy vetch has strong allelopathic effect on alfalfa and o-coumaric acid is a chemical growth inhibitor.

scholarly journals Study of Mitotic Activity and Chromosomal Behaviour in Root Meristem of Allium cepa L. Treated with Magnesium Sulphate

2009 ◽  
Vol 15 ◽  
pp. 83-88 ◽  
Author(s):  
Prabhakar Bhatta ◽  
S. R. Sakya
Keyword(s):  
Key Words ◽  
Mitotic Activity ◽  
Allium Cepa ◽  
Biological System ◽  
Magnesium Sulphate ◽  
Root Meristem ◽  
Chromosomal Abnormalities ◽  
Inhibitory Effect ◽  
Duration Of Treatment ◽  
Chromosomal Behaviour

The mitotic activity and chromosomal behaviour was observed in four different concentrations of magnesium sulphate at four different duration of treatment using Allium cepa as a biological system. Mito-inhibitory effect of the compound was observed in higher concentration. The compound was capable of producing various chromosomal abnormalities indicating the cytostatic and clastogenic properties of magnesium sulphate. Key words: Allium cepa, magnesium sulphate, mitotic index, phase indices.doi: 10.3126/eco.v15i0.1947 ECOPRINT 15: 83-88, 2008

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