scholarly journals CLINICOHEMATOLOGICAL, IMMUNOPHENOTYPING, MOLECULAR PROFILE, AND OVERALL SURVIVAL IMPACT IN ACUTE LYMPHOID LEUKEMIA PATIENTS FROM NORTH INDIA

2019 ◽  
pp. 164-171
Author(s):  
MANOJ KUMAR ◽  
MOHIT CHOWDHRY ◽  
RAJ NATH MAKROO ◽  
DEEPIKA RANI ◽  
VANDANA SHARMA ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Chromosomal Abnormalities ◽  
Lymphoblastic Leukemia ◽  
Tertiary Care ◽  
North India ◽  
Molecular Profile ◽  
Pathophysiological Mechanism ◽  
Care Hospital ◽  
Molecular Subgroup ◽  
Lymphoid Leukemia

Objective: Cytogenetic plays an inevitable role in predicting the diagnosis of acute leukemia. The recurrent chromosomal aberrations in acute leukemia have provided critical insights into the pathophysiological mechanism of leukemogenesis. Cytogenetics findings at diagnostics provide important information for decision-making in both childhood and adult acute lymphoblastic leukemia (ALL). The cure rate for ALL is >80% in children and 35% in adults. Despite the therapeutic advances in ALL, several important biological and pathophysiological questions remain to be answered to achieve an accurate diagnosis, timely prognosis, and maximum therapeutic benefit. Methods: The present study was carried out at tertiary care hospital, New Delhi, India. A total of 144 newly diagnosed ALL patients were analyzed for clinicohematological profile, immunophenotyping, conventional, and molecular cytogenetics. Results: The study population was found to have normal karyotypes in most of the cases; however, abnormalities also reported. Our study clearly indicates that the application of fluorescence in situ hybridization has increased sensitivity and accuracy for detecting various chromosomal abnormalities, more so with the cryptic rearrangements. Conclusion: We observed that the prevalence of the molecular subgroup of leukemia with a potential for a favorable clinical outcome (ETV6-RUNX1 and hyperdiploidy) in precursor B-ALL is higher in the North India.

scholarly journals Simultaneous or sequential expression of lymphoid and myeloid phenotypes in acute leukemia

Blood ◽  
1985 ◽  
Vol 65 (1) ◽  
pp. 142-148 ◽  
Author(s):  
PB Neame ◽  
P Soamboonsrup ◽  
G Browman ◽  
RD Barr ◽  
N Saeed ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Myeloid Leukemia ◽  
Chromosomal Abnormalities ◽  
Lymphoblastic Leukemia ◽  
Differentiation Markers ◽  
Lymphoid Leukemia ◽  
Chromosome Marker ◽  
Fab Classification ◽  
Blast Cells ◽  
French American British

Abstract Acute mixed myeloid-lymphoid leukemia is uncommon. We report four cases in which myeloid and lymphoid cell markers were observed simultaneously or sequentially when 94 patients with acute leukemia were phenotyped according to the French-American-British (FAB) classification system, with cytochemical stains, and with immunologically defined differentiation markers (identified by monoclonal antibodies and antiterminal deoxynucleotidyl transferase [TdT]). In one case, conversion from acute lymphoblastic leukemia to acute myeloid leukemia was noted (FAB L1, TdT+ to FAB M4, Auer rods, TdT-). In another patient, two distinct populations of myeloid and lymphoid blast cells were observed simultaneously (TdT-, LeuM1+/TdT+, LeuM1-). In two additional patients, acute leukemia was characterized by the expression of both lymphoid and myeloid markers on the same cell (TdT+/Leu M1+, B4+/Leu M1+ and greater than or equal to 70% TdT+, T11+, My9+). The Philadelphia (Ph1) chromosome was negative in all cases, though other chromosomal abnormalities were noted in three out of four cases. Malignant transformation of a pluripotential stem cell for both lymphoid and myeloid lineages, with or without the Ph1 chromosome marker, could explain the coexistence of distinct populations of lymphoblasts and myeloblasts in acute leukemia. Acute leukemia with a biphenotypic profile may reflect genome depression accompanying neoplasia.

scholarly journals ACUTE LYMPHOBLASTIC LEUKEMIA; CHROMOSOMAL ABNORMALITIES IN CHILDHOOD REPORTING AT A TERTIARY CARE HOSPITAL OF SINDH

2016 ◽  
Vol 23 (03) ◽  
pp. 312-316
Author(s):  
Dr. Ghulam Shah Nizzamani ◽  
Zaheer Ahmed Nizamani ◽  
Dr. Amin Fahim ◽  
Dr. Ikram Uddin Ujjan
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Chromosomal Abnormalities ◽  
Tertiary Care Hospital ◽  
Lymphoblastic Leukemia ◽  
Tertiary Care ◽  
Care Hospital

scholarly journals Acute Lymphoblastic Leukemia: Fourteen Years Experience of a Single Institution

2014 ◽  
Vol 34 (1) ◽  
pp. 1-6 ◽  
Author(s):  
KP Sah ◽  
PN Shrestha
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Acute Leukemia ◽  
Remission Rate ◽  
Lymphoblastic Leukemia ◽  
Tertiary Care ◽  
Malignant Neoplasm ◽  
Bone Marrow Aspiration ◽  
Care Hospital ◽  
Female Ratio ◽  
Oncology Unit

Introduction: Leukemia commonly known as blood cancer is the most common malignant neoplasm in childhood accounting for about 41 % of all malignancies that occur in children younger than 15 year of age. The objectives of this study were to find out the clinico-laboratory features and survival of children with acute lymphoblastic leukemia (ALL) during fourteen years in pediatric oncology unit of a tertiary care hospital. Materials and Methods: This was a retrospective study conducted at Kanti Children’s Hospital (KCH) from March 1998 to March 2012. Bone marrow aspiration showing ≥25 % blast cells was the criteria for diagnosis of ALL. Results: Out of 755 childhood cancers reported in this hospital during study period, total number of Acute leukemia patients were 375 (49.7%). Among acute leukemia, patients with ALL were 300, which was 80.0 % among all leukemias and 39.7% of all cases of cancers. Among cases of ALL, L1, L2 and L3 constituted 163 (54.3%), 131 (43.7%) and 6 (2%) respectively. The age of the children with acute leukemia ranged from six months to fourteen years, with a mean age of 7.3 years. The majority of children (61.7 %) with ALL fell into the age group of 2-9 years. Males: Female ratio was (M:F=1.3:1 ). The most common presenting features in ALL were fever (89.2 %), followed by splenomegaly (89.1%), hepatomegaly (69.2%) and lymphadenopathy (58.4 %). Among all patients, remission rate was 28.3% at ≥ 5 years, 17.7% were on maintenance, 30.3% abandoned treatment and 23.7 % died. Conclusion: This study showed that the patients on remission at ≥ 5 years in this centre were 28.3%. DOI: http://dx.doi.org/10.3126/jnps.v34i1.9056 J Nepal Paediatr Soc 2014;34(1):1-6

scholarly journals Simultaneous or sequential expression of lymphoid and myeloid phenotypes in acute leukemia

Blood ◽  
1985 ◽  
Vol 65 (1) ◽  
pp. 142-148 ◽  
Author(s):  
PB Neame ◽  
P Soamboonsrup ◽  
G Browman ◽  
RD Barr ◽  
N Saeed ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Myeloid Leukemia ◽  
Chromosomal Abnormalities ◽  
Lymphoblastic Leukemia ◽  
Differentiation Markers ◽  
Lymphoid Leukemia ◽  
Chromosome Marker ◽  
Fab Classification ◽  
Blast Cells ◽  
French American British

Acute mixed myeloid-lymphoid leukemia is uncommon. We report four cases in which myeloid and lymphoid cell markers were observed simultaneously or sequentially when 94 patients with acute leukemia were phenotyped according to the French-American-British (FAB) classification system, with cytochemical stains, and with immunologically defined differentiation markers (identified by monoclonal antibodies and antiterminal deoxynucleotidyl transferase [TdT]). In one case, conversion from acute lymphoblastic leukemia to acute myeloid leukemia was noted (FAB L1, TdT+ to FAB M4, Auer rods, TdT-). In another patient, two distinct populations of myeloid and lymphoid blast cells were observed simultaneously (TdT-, LeuM1+/TdT+, LeuM1-). In two additional patients, acute leukemia was characterized by the expression of both lymphoid and myeloid markers on the same cell (TdT+/Leu M1+, B4+/Leu M1+ and greater than or equal to 70% TdT+, T11+, My9+). The Philadelphia (Ph1) chromosome was negative in all cases, though other chromosomal abnormalities were noted in three out of four cases. Malignant transformation of a pluripotential stem cell for both lymphoid and myeloid lineages, with or without the Ph1 chromosome marker, could explain the coexistence of distinct populations of lymphoblasts and myeloblasts in acute leukemia. Acute leukemia with a biphenotypic profile may reflect genome depression accompanying neoplasia.

Clinical and molecular profile of patients with gonadal dysgenesis attending tertiary care hospital.

2014 ◽  
Author(s):  
Vasundhera Chauhan ◽  
Madan Lal Khurana ◽  
Poonam Gupta ◽  
Iram Sabir ◽  
A.C Ammini
Keyword(s):  
Gonadal Dysgenesis ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Molecular Profile ◽  
Care Hospital

The Spectrum of Skin Biopsies from a Tertiary Care Hospital in North India

2016 ◽  
Vol 2 (5) ◽  
Author(s):  
Abhilasha Williams ◽  
Anuradha Bhatia ◽  
EmyAbi Thomas ◽  
Clarence J Samuel
Keyword(s):  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
North India ◽  
Care Hospital ◽  
Skin Biopsies

Seroprevalence of anti-SARS-CoV-2 IgG antibodies in hospitalized patients at a tertiary referral center in North India (Preprint)

2020 ◽  
Author(s):  
Dr. Animesh Ray ◽  
Dr. Komal Singh ◽  
Souvick Chattopadhyay ◽  
Farha Mehdi ◽  
Dr. Gaurav Batra ◽  
...  
Keyword(s):  
Tertiary Care ◽  
Age Groups ◽  
Hospitalized Patients ◽  
North India ◽  
P Value ◽  
Care Hospital ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Increased Risk ◽  
Significant Difference

BACKGROUND Seroprevalence of IgG antibodies against SARS-CoV-2 is an important tool to estimate the true extent of infection in a population. However, seroprevalence studies have been scarce in South East Asia including India, which, as of now, carries the third largest burden of confirmed cases in the world. The present study aimed to estimate the seroprevalence of anti-SARS-CoV-2 IgG antibody among hospitalized patients at one of the largest government hospital in India OBJECTIVE The primary objective of this study is to estimate the seroprevalence of SARS-CoV-2 antibody among patients admitted to the Medicine ward and ICU METHODS This cross-sectional study, conducted at a tertiary care hospital in North India, recruited consecutive patients who were negative for SARS-CoV-2 by RT-PCR or CB-NAAT. Anti-SARS-CoV-2 IgG antibody levels targeting recombinant spike receptor-binding domain (RBD) protein of SARS CoV-2 were estimated in serum sample by the ELISA method RESULTS A total of 212 hospitalized patients were recruited in the study with mean age (±SD) of 41.2 (±15.4) years and 55% male population. Positive serology against SARS CoV-2 was detected in 19.8%patients(95% CI 14.7-25.8). Residency in Delhi conferred a higher frequency of seropositivity 26.5% (95% CI 19.3-34.7) as compared to that of other states 8% (95% CI 3.0-16.4) with p-value 0.001. No particular age groups or socio-economic strata showed a higher proportion of seropositivity CONCLUSIONS Around, one-fifth of hospitalized patients, who were not diagnosed with COVID-19 before, demonstrated seropositivity against SARS-CoV-2. While there was no significant difference in the different age groups and socio-economic classes; residence in Delhi was associated with increased risk (relative risk of 3.62, 95% CI 1.59-8.21)

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