THE RELATIONSHIP BETWEEN GDF-9 AND BMP-15 SERUM AND FOLLICULAR FLUID AND THE QUALITY OF OOCYTES IN WOMEN WHO UNDERGO AN IVF CYCLE

2020 ◽  
pp. 22-27
Author(s):  
ZAINAL ARIFIN ◽  
KANADI SUMAPRADJA ◽  
UPIK ANGGRAENI ◽  
NAYLAH MUNA ◽  
BUDI WIWEKO
Keyword(s):  
Follicular Fluid ◽  
Statistical Correlation ◽  
Average Level ◽  
Vitro Fertilization ◽  
Fertilization Rates ◽  
Secreted Factors ◽  
The Mean ◽  
The Relationship

Objective: The quality of the embryo is greatly influenced by the quality of the oocytes; oocyte-secreted factors (OSFs), which include GDF-9 and BMP-15, play an important role in folliculogenesis. This study was to determine the relationship between GDF-9 and BMP-15 serum within the follicular fluid in order to predict the quality of oocytes in women undergoing In vitro Fertilization (IVF). Methods: We collected 30 samples of blood serum and 30 samples of follicular fluid on the day of ovum pickup (OPU), and examined GDF-9 and BMP-15 using ELISA kits. Analysis by Pearson and a partial-correlation was conducted to analyze the correlation between the concentration of GDF-9 and BMP-15 in serum and follicular fluid with general physiological parameters, such as maturation and fertilization rates. Results: The mean age of the subjects was 35,0(26,0-39,0) years. There was no statistical correlation between GDF-9 serum and follicular fluid (p=0.245); but there was a correlation between BMP-15 serum and follicular fluid (p=0.001). Average level of GDF-9 in the follicular fluid was 163,0 pg/ml (48,0-537), and average level in the serum was 260.33 pg/ml±121,82; average levels of BMP-15 in the follicular fluid was 58.30pg/ml±31,54 and average levels of BMP-15 in the serum was 74.20 pg/ml (1,0–610). Conclusion: There were no correlations between levels of GDF-9 serum-FF and BMP-15-FF, and maturation rates and fertilization rates. There was a negative correlation between BMP-15 serum and maturation rates.

scholarly journals Association between Follicular Fluid Leptin and Serum Insulin Levels in Nonoverweight Women with Polycystic Ovary Syndrome

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
G. Garruti ◽  
R. de Palo ◽  
M. T. Rotelli ◽  
S. Nocera ◽  
I. Totaro ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Serum Insulin ◽  
Fertilization Rate ◽  
Serum Leptin ◽  
Vitro Fertilization ◽  
Insulin Levels ◽  
Fertilization Rates ◽  
And Control

Aims. We evaluated the links between leptin and visfatin levels and fertilization rates in nonoverweight (NOW) women with PCOS (NOW-PCOS) from Apulia undergoing in vitro fertilization/embryo transfer (IVF).Materials and Methodology. We recruited 16 NOW women with PCOS (NOW-PCOS) and 10 normally ovulating NOW women (control-NOW). All women underwent IVF. Androgens, 17-β-estradiol (17β-E2), and insulin levels were measured in plasma and/or serum and leptin and visfatin levels were assayed in both serum and follicular fluid (FF-leptin, FF-visfatin).Results. In NOW-PCOS, both serum and FF-leptin were significantly lower than in control-NOW. In NOW-PCOS, significant correlations were found between BMI and serum leptin and insulinemia and FF-leptin. By contrast, in control-NOW, FF-leptin levels were not correlated with insulinemia. Serum visfatin levels were not significantly different in NOW-PCOS and control-NOW, but FF-visfatin levels were 1.6-fold higher, although not significantly, in NOW-PCOS than in control-NOW.Conclusions. Both serum leptin levels and FF-leptin are BMI- and insulin-related in Southern Italian NOW-PCOS from Apulia. In line with other reports showing that FF-leptin levels are predictive of fertilization rates, lower than normal FF-leptin levels in NOW-PCOS may explain their lower fertilization rate and this may be related to the level of insulin and/or insulin resistance.

scholarly journals The relationship between follicular fluid aspirate volume and oocyte maturity in in-vitro fertilization cycles

1998 ◽  
Vol 13 (7) ◽  
pp. 1901-1906 ◽  
Author(s):  
O. Salha ◽  
D. Nugent ◽  
T. Dada ◽  
S. Kaufmann ◽  
S. Levett ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Oocyte Maturity ◽  
Vitro Fertilization ◽  
The Relationship

Micro-assisted fertilization

1995 ◽  
Vol 7 (4) ◽  
pp. 831 ◽  
Author(s):  
D Payne
Keyword(s):  
Poor Quality ◽  
Male Factor Infertility ◽  
Male Factor ◽  
Vitro Fertilization ◽  
Fertilization Rates ◽  
Partial Zona Dissection ◽  
Oocyte Cytoplasm ◽  
Assisted Fertilization

In couples who undergo routine in vitro fertilization (IVF), 17% experience significant problems with fertilization and many others are unable to have routine IVF because the quality of their semen is too poor. Often, the only options previously available to these couples were to use sperm donated by fertile men or to remain childless. Micromanipulative assisted fertilization techniques have improved the treatment of severe male factor infertility significantly and this paper provides a brief overview of the recent methodologies. Initially, techniques such as zona drilling and partial zona dissection, in which a hole or slit is placed in the zona pellucida, demonstrated that fertilization and pregnancies could be achieved with semen of very poor quality, but successes were sporadic. Later, subzonal injection of spermatozoa provided more consistent results with many units reporting pregnancies; however, relatively low rates of fertilization (14-34%) and high rates of polyspermy remained unresolved problems. The latest technique, the injection of a single spermatozoon into the oocyte cytoplasm, although technically difficult in animal models, proved to be highly successful in the human, restoring fertilization rates to those seen in routine IVF (65%) and producing good pregnancy rates from transferred embryos. Intracytoplasmic sperm injection has become the method of choice in the treatment of severe male factor infertility and preliminary data suggest that there is no increase in congenital abnormality among babies born after the transfer of injected oocytes.

scholarly journals Blood serum and follicular fluid relaxin: a pilot study of the hormone effects on ovarian function and fertilization efficiency

2020 ◽  
Vol 69 (5) ◽  
pp. 59-68
Author(s):  
Olesya N. Bespalova ◽  
Valeriya A. Zagaynova ◽  
Olga V. Kosyakova ◽  
Alexander M. Gzgzyan ◽  
Igor Yu. Kogan ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Blood Serum ◽  
Body Mass ◽  
Follicular Fluid ◽  
Ovarian Function ◽  
Endometrial Thickness ◽  
Vitro Fertilization ◽  
Final Maturation

Hypothesis/aims of study. To date, one of the most important avenues of research in the field of reproductive medicine is the searching for new biochemical markers of oocyte quality and the prediction of the effectiveness of in vitro fertilization (IVF) protocols. The aim of this study was to assess the effect of relaxin levels in blood serum and follicular fluid on the efficiency of ovulation stimulation, fertilization, and characteristics of the embryos. Study design, materials and methods. This prospective randomized cohort study included 11 patients undergoing infertility treatment in a superovulation stimulation protocol using gonadotropin-releasing hormone antagonists. Age, body mass index, hormonal status, ovarian response, endometrial thickness and structure, the number and quality of oocytes and embryos, as well as fertilization efficiency were assessed. The level of relaxin in blood serum and follicular fluid samples was determined on the day of transvaginal follicle puncture using enzyme immunoassay. Results. A correlation between follicular fluid relaxin levels and body mass index, age, the number of oocytes, and their fertilization efficiency (p 0.05) was established. Changes in follicular fluid relaxin level were revealed depending on the gonadotropin preparations (p 0.05) and triggers of final maturation of oocytes (p 0.05). The tendency of the effect of gonadotropin doses on circulating relaxin levels, and of the hormone itself on endometrial thickness and the quality of oocytes was determined. Conclusion. Determination of the relaxin concentration can be considered as a promising method for predicting the result of ovarian stimulation and the efficiency of fertilization in IVF protocols.

scholarly journals Study on follicular fluid metabolomics components at different ages based on lipid metabolism

2020 ◽  
Author(s):  
xingxing zhang ◽  
tianqi wang ◽  
jingyan song ◽  
jifeng deng ◽  
Zhengao Sun
Keyword(s):  
Lipid Metabolism ◽  
Follicular Fluid ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Age Groups ◽  
Principal Component ◽  
Vitro Fertilization ◽  
Different Ages

Abstract Background: Follicular fluid is an important external environment for the growth and development of oocytes. A thorough identification of specific components in follicular fluid can better the existing understand of intracellular signal transduction and reveal potential biomarkers of oocyte health in women undergoing assisted reproductive therapy. To study on follicular fluid metabolomics components at different ages based on lipid metabolism, we have adopted a new method of SWATH to MRM( the sequential window acquisition of all theoretical fragment-ion spectra to multiple reaction monitor)metabolomics to provide extensive coverage and excellent quantitative data. This was done to investigate the differences in follicular fluid of patients undergoing in vitro fertilization (IVF) and embryo transfer in different age groups and to further explore the relationship between follicular fluid, age and reproductive function.Method: A combination of Ultra-high-performance liquid chromatography and high resolution mass spectrometry techniques were used to analyze the follicular fluid of 230 patients enrolled for the IVF cycle. The patients were of different ages grouped into two groups:the younger and older patients.The obtained multidimensional chromatographic data were processed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The charge ratios and mass numbers enabled for the identification of different fragments in the samples. Matching information obtained through database search and the fragment information obtained by fragment ion scan structurally identified substances in the samples. This was used to determine the differential compounds.Results:The quality of oocytes decline with age,and the lipid composition in follicular fluid also changes,The lipid metabolism that changes with age may be related to the quality of oocytes.The main differences were in lipid metabolites. Some were up-regulated : Arachidonate, LysoPC(16:1), LysoPC(20:4) and LysoPC(20:3) while others were down-regulated: LysoPC(18:3) and LysoPC(18:1).Conclusions: Metabolomic analysis of follicular fluid revealed that with the increase in age, several differential metabolites are at play. Among these metabolites, lipid metabolism undergoes significant changes that affect the development of oocytes thus causing reduced fertility in older women. These differential metabolites related to follicular development may provide possible detection and treatment targets for promoting oocyte health, and provide scientific basis for understanding the environment of oocyte development.

154 CRYO-ACROSOME REACTION/CAPACITATION OF EJACULATED AND EPIDIDYMAL BOVINE SPERM AND THEIR IN VITRO FERTILIZATION RATES

2012 ◽  
Vol 24 (1) ◽  
pp. 189
Author(s):  
M. A. Stout ◽  
J. R. Saenz ◽  
J. F. Chenevert ◽  
G. T. Gentry ◽  
K. B. Bondioli ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Fertilization Rate ◽  
Fluorescent Staining ◽  
Epididymal Sperm ◽  
Vitro Fertilization ◽  
Mean Values ◽  
Fertilization Rates ◽  
Bovine Sperm ◽  
The Mean

Seminal plasma has been shown to affect the composition and function of sperm. This exposure may alter the ability of sperm to endure cryopreservation, undergo capacitation and fertilize oocytes. Earlier studies demonstrated that the freezing response of epididymal and ejaculated sperm from the bulls utilised in these studies was similar (Alapati et al. 2009). Subsequent studies are designed to investigate the response of ejaculated and epididymal bovine sperm to cryopreservation and their ability to undergo capacitation. Ejaculated and epididymal sperm from the same bulls (n = 4) were collected by an artificial vagina and retrograde caudal epididymal flush, respectively and were cryopreserved in standard egg-yolk glycerol extender. Sperm was compared by level of cryo-acrosome reaction/capacitation and their fertilization rates in vitro with and without the capacitating agent heparin. Upon evaluation, a sample was thawed and washed and the viable sperm population was isolated through a discontinuous Percoll® gradient. Sperm viability and acrosome status were assessed by fluorescent staining with propidium iodine and fluorescein isothiocyanate-PNA followed by evaluation with flow cytometry. Capacitated sperm were then induced to undergo the acrosome reaction by exposure to lysophosphatidylcholine (10 μg mL–1). Cryo-capacitation was determined by the difference between cryo- and lysophosphatidylcholine-induced acrosome-reacted sperm levels. Differences in the mean values between groups were analysed by ANOVA. Ejaculated and epididymal sperm differed by level of cryo-acrosome reaction (15 vs 4%, respectively; P < 0.001), but did not differ by level of cryo-capacitation (6.2 vs 5.9%, respectively; P = 0.92). The ability of epididymal and ejaculated sperm to fertilize oocytes in vitro with and without heparin was also investigated. Sperm were thawed and washed and the viable population was isolated through a Percoll® gradient. Oocytes were washed and randomly assigned to a treatment group, either ejaculated ± heparin or epididymal ± heparin. Oocytes and sperm were added to a capacitating medium (TALP) with and without heparin. Following 18 h of incubation, fertilization rate was determined by pronuclear fluorescent staining with Hoechst 33342 followed by confirmation with aceto-orcein staining. Differences in the mean values among treatment groups were analysed by one-way ANOVA followed by Holm-Sidak pairwise multiple comparisons. Fertilization rates of epididymal sperm with and without heparin and ejaculated sperm with heparin were similar (76, 80 and 70%, respectively). Ejaculated sperm without heparin was lower than all other groups, with a fertilization rate of 42% (P < 0.001). In summary, epididymal sperm displayed lower levels of cryo-acrosome reaction but were similar by level of cryo-capacitation. In addition, epididymal and ejaculated sperm differed in the need for a capacitating agent such as heparin when used in vitro. This may be useful to increase efficiency when using epididymal sperm in assisted reproductive techniques.

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