scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF LEAF EXTRACTS OF GREWIA HETEROTRICHA MAST

2016 ◽  
Vol 8 (4) ◽  
pp. 68 ◽  
Author(s):  
B. Usha ◽  
K. C. Pushpalatha
Keyword(s):  
Aqueous Extract ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts

Objective: To investigate the presence of phytochemical components and to evaluate the in vitro antioxidant activity of pet. ether, chloroform, methanol and aqueous extracts of Grewia heterotricha mast leaves.Methods: The leaves of Grewia heterotricha mast were dried and extracts were prepared using a pet. ether, chloroform, methanol by soxhlet extraction method. The aqueous extract was prepared using distilled water by cold extraction method. The preliminary phytochemical analysis was carried out on aqueous, methanol, chloroform and pet. either leaf extracts of the plant using standard qualitative procedures. The total phenolic content (TPC) was estimated using modified Folin-Ciocalteau method, tannin content by Folin-Denis method and total flavonoids by aluminum chloride method. In vitro, antioxidant activities were evaluated by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, H2O2 scavenging activity and FRAP assay.Results: The preliminary phytochemical analysis revealed the presence of complex bioactive constituents like phenols, tannins, alkaloids, terpenoids, flavonoids, saponins, steroids, glycosides, coumarins, proteins and carbohydrates. Methanolic extract showed highest total phenolic content (87.58±2.52 mg CE/g) than aqueous extract (78.46±5.36 mg CE/g). Higher tannin content was found in the aqueous extract (148.0±8.96 mg TAE/g). Total flavonoids were highest in chloroform extract (314.9±25.06 mg QE/g) followed by aqueous (242.98±32.42 mg QE/g) and methanolic extract (217.0±18.32 mg QE/g) and lowest in a pet. ether extract (188.86±23.35 mg QE/g). The methanolic extract had shown very significant DPPH radical scavenging activity (IC50 98.95 µg/ml) and H2O2 scavenging activity (IC50110.1µg/ml) compared to the standard ascorbic acid. Higher reducing ability was observed in methanol extract (131.8±11.67 mg AE/g).Conclusion: The results obtained reveal that the leaves of Grewia heterotricha mast have potent antioxidant property. The observed activity may be associated with bioactive components like phenolics, flavonoids present in the leaf extracts and could have greater importance as therapeutic agents in oxidative stress-related degenerative diseases. Further studies are needed in order to purify bioactive compounds responsible for the antioxidant property.

scholarly journals Phytochemical Screening and in vitro Antioxidant Properties of Methanol and Aqueous Leaf Extracts of Geophila obvallata

2018 ◽  
pp. 1-11
Author(s):  
Iserhienrhien Lucky Osafanme ◽  
Okolie Paulinus Ngozi
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scientific Basis ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Reducing Ability

Aim: This study investigated the phytochemical constituents and in vitro antioxidant properties of methanol and aqueous leaf extracts of Geophila obvallata using standard methods. Materials and Methods: The in vitro antioxidant assays carried out were 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging ability, Nitric oxide (NO•) radical scavenging activity assay, 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) radical cation scavenging assay, ferric reducing properties and hydroxyl radical scavenging assays. Results: Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, steroids, saponins, terpernoids and cardiac glycosides in both extracts. Relative to the aqueous extract, the methanol extract contained a higher amount of the secondary metabolites. However, both extracts exhibited appreciable and dose-dependent capacities for quenching DPPH, ABTS•+ and NO• free radicals, and potent ferric reducing ability to levels comparable to those of ascorbic acid. The crude methanol extract showed significantly increased (P<0.05) antioxidant activity than the aqueous extract. Conclusion: It was concluded that the extract possesses strong antioxidant properties due to its content of phytochemicals, and provides scientific basis for its ethno medicinal applications.

scholarly journals In-vitro Antioxidant Activity and Preliminary Phytochemical Analysis of Different Leaf Extracts of Hemionitis arifolia

2021 ◽  
pp. 281-291
Author(s):  
Rajendran Raja Priya ◽  
N. Bhadusha ◽  
Veramuthu Manivannan ◽  
Thanthoni Gunasekaran
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Hydroalcoholic Extract ◽  
Reducing Power Assay

Objective: To evaluate the preliminary phytochemical content and antioxidant potential of the hydroalcoholic leaf extracts of Hemionitis arifolia. Methods: Total phenolic, flavonoid and alkaloid contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the leaf hydroalcoholic extract were evaluated against DPPH+, ABTS+, Reducing power assay and nitric oxide assay were determined. Results: The hydroalcoholic concentrate of H. arifolia uncovered the most elevated polyphenol content when contrasted and the other phytoconstituents. Absolute phenol content of the hydroalcoholic separate was observed to be 31.78%, flavonoid content is 1.02% and Alkaloid content is 30.40% individually. The Solvent concentrates showed huge cell reinforcement movement, with hydroalcoholic extract. ABTS Assay, DPPH assay, Reducing power assay and Nitric oxide assay where the Inhibition concentration were 667.75µg/ml, 734.25 µg/ml, 791.58 µg/ml and 899.67 µg/ml. Conclusion: This study suggests that hydroalcoholic leaf extracts of H. arifolia could be a potential source of natural antioxidant and justifies its use in ethno-medicine.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF LEAVES OF CORCHORUS OLITORIUS

2017 ◽  
pp. 59-63
Author(s):  
Abdul Sadat ◽  
Mayukh Hore ◽  
Kaushik Chakraborty ◽  
Subhrajyoti Roy
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Objective: The present study was carried out to evaluate the in vitro preliminary phytochemical analysis and antioxidant activity of methanolic leaf extracts of Corchorus olitorius L. The in vitro antioxidant activity was evaluated by DPPH radical scavenging activity method.Methods: Fresh jute leaves (immature, mature and senescence) were collected, air dried and the crude powder was prepared for phytochemical and antioxidant analysis. The powder was mixed with 70% methanol and the supernatant was separated. The antioxidant activity of this methanolic extract was measured on the basis of the scavenging activity of the stable 1, 1-diphenyl 2-picrylhydrazyl (DPPH) free radical with slight modifications. Phytochemical analysis was performed according to standard laboratory protocol.Results: The results indicated the presence of different phytochemicals viz. glycosides, steroids, cholesterol, alkaloids, phenols, flavonoids, riboflavin, saponins and terpenoids. The sample also showed antioxidant activity by inhibiting DPPH radical. The significant antioxidant activity of methanolic leaf extract of might be due to the presence of saponins, phenols, flavonoids and alkaloids found in the preliminary phytochemical analysis.Conclusion: Present study reveals that the jute leaf possesses different phytonutrients and exhibited DPPH radical scavenging activity, and therefore, may be used for therapeutic purposes.

scholarly journals PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITY OF CLERODENDRUM PANICULATUM (L.) LEAF AND FLOWER EXTRACTS

2021 ◽  
pp. 31-34
Author(s):  
DEEPA R HEBBAR ◽  
DEVIKA M ◽  
RASHMI GN ◽  
NALINI MS
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Hexane Extracts ◽  
Reducing Power Assay

Objective: This study was designed to evaluate the phytochemicals present in the flower and leaf extracts of Clerodendrum paniculatum L., collected from Nelji village of Kodagu district . Methods: The healthy leaves and flowers of C. paniculatum were collected and the plant extracts were prepared using ethanol, hexane and distilled water separately. Phytochemical analysis was conducted using standard procedurs for the flower and leaf extracts of C. paniculatum. The antioxidant activity in leaf and flower extracts was determined by three assays, estimation of total phenolic content, reducing power assay and radical scavenging activity (DPPH) using standard procedures. Results: Phytochemical screening conducted for the flower and leaf extracts of C. paniculatum showed the presence of three phytochemicals, namely saponins, alkaloids and terpenoids. Terpenoids were commonly present in all the extracts of flower and leaf that is in both polar (aqueous and ethanol) solvent and in non-polar (hexane) solvents. The extracts tested for the antioxidant activity showed the presence of total phenolics in ethanol, aqueous and hexane extracts. The aqueous extract showed high redox potential followed by ethanol and hexane extracts. The aqueous leaf extract showed high radical scavenging activity when compared to the flower extracts of C. paniculatum. Conclusion:  The present study showes C. paniculatum to be an important medicinal plant, since the flower and leaves showed good antioxidant activity. Thus it may used in the treatment of diseases and may also used in the preparation of natural or herbal drugs due to the presence of antioxidants.

scholarly journals Antioxidant, anti-inflammatory, analgesic and anti-proliferating activities of Grewia heterotricha Mast.

Biomedicine ◽  
2020 ◽  
Vol 40 (3) ◽  
pp. 272-277
Author(s):  
B. Usha ◽  
Karanth Jyothsna ◽  
Joshi Chandrashekhar G.
Keyword(s):  
Aqueous Extract ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dpph Radical Scavenging Activity ◽  
Leaf Extracts ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Introduction and Aim: Plants are considered to be novel source of active compounds having pharmacological properties and help in the development of therapeutic agents. Hence, this study was undertaken to evaluate the antioxidant, anti-inflammatory, analgesic and anti-proliferating activity of aqueous and methanolic leaf extracts of Grewia heterotricha Mast. Materials and Methods: The aqueous and methanolic leaf extracts of the plant were assessed for their in-vitro antioxidant activity by DPPH radical scavenging activity, in-vivo anti-inflammatory activity by carrageenan induced rat paw edema method, in-vivo analgesic activity by acetic acid-induced writhing test and in-vitro anti- proliferating activity by MTT assay. Results: The methanolic extract had shown very significant DPPH radical scavenging activity with IC50 value 98.95?g/ml than aqueous extract and showed a significant reduction in the paw volume of rats at the concentration of 100 mg/kg body weight indicating potent anti-inflammatory activity compared with the reference standard Diclofenac sodium. Both the extracts showed significant analgesic effect (p<0.001) in acetic acid-induced pain models in a dose dependent manner. The methanolic extract showed higher analgesic activity compared to aqueous extract by inhibiting the pain indicated by a decrease in the number of writhes. In addition, both the extracts showed a decrease in MCF-7 cell viability at the concentration of 550µg/ml. Compared to the aqueous extract, MEGH has shown more cytotoxic effect on the cancer cell lines. Conclusion: The results suggest that both aqueous and methanolic extracts of Grewia heterotricha Mast. leaves possess potent antioxidant, analgesic, anti-inflammatory and anti-proliferating properties, which supports the use of the plant in traditional medicine. Further investigation is required to illuminate on its active compounds.   Keywords: Analgesic; anti-inflammatory; DPPH; cytotoxic.

Strong antihemolytic and antioxidant properties of aqueous extract from Algerian Hammada elegans (Bge.) Botsch (Chenopodiaceae)

2021 ◽  
Vol 17 ◽  
Author(s):  
Brahim Asseli ◽  
Reguia Mahfoudi ◽  
Amar Djeridane ◽  
Mohamed Yousfi
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Condensed Tannin ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Vitro Assay

Background: Research on medicinal plant antioxidants has emerged as a potential therapeutic to prevent free radical generated damage in the human body. Hammada elegans Botsch (popularly known as “Ajram”) is a xerophytic plant widely found in Laghouat region, but there are only a few reports about the biological or chemical properties of these species. Hence, the aim of this study is to investigate the antioxidant and the antihemolytic activities of hexanic, acetonic, methanolic and aqueous extracts of aerial parts of Algerian Hammada elegans Botsch by employing different in vitro assay systems. Methods: The total phenolic content, the flavonoid content and the condensed tannin amount were analyzed using Folin-Ciocalteu, aluminum chloride and vanillin assays, respectively. The in vitro antioxidant capacity of extracts was assessed by CUPRAC, iron chelating, ABTS•+and antihemolytic assays, and was expressed as EC50 values. Results: Among the analyzed extracts, the aqueous extract had the highest phenolic, flavonoid and tannin contents. Also, this extract displayed the highest antioxidant capacities compared to the other extracts and standards. Its EC50 value for ABTS radical-scavenging activity was 0.265 ± 0.003 mg/L. Moreover, this extract showed high iron (II) chelating ability (EC50 = 0.958 ± 0.001 mg/L), and good antioxidant activity in the cupric ion reducing activity (CUPRAC) in a concentration dependent manner (EC50 were 0.709 ± 0.002 mg/L). Additionally, this extract had the best antihemolytic activity against AAPH-induced hemolysis (EC50=0.090 ± 0.004 mg/L). Conclusion: Our study revealed that the aqueous extract of Hammada elegans Botsch, is a potential source of antioxidants which possess a high protective effect of membrane against free radical.

In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 75-79
Author(s):  
S.O. Olubodun ◽  
G.E. Eriyamremu ◽  
M.E. Ayevbuomwan ◽  
C.I. Nzoputa
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

scholarly journals ANTIOXIDANT ACTIVITY OF LEAF EXTRACTS OF HEMIDESMUS INDICUS (L.) R.BR. (ASCLEPIADACEAE).

2017 ◽  
Vol 10 (6) ◽  
pp. 200
Author(s):  
Abdul Kaffoor H ◽  
Muthuraj K ◽  
Arumugasamy K
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Stable Free Radical ◽  
Hemidesmus Indicus

Objective: A number of Indian medicinal plants have been used for thousands of years in a traditional system of medicine. Hemidesmus indicus is an important member of the Asclepiadaceae family. It is an endemic to the southern Western Ghats, India. The aim of the study was to investigate the free radical scavenging activity of H. indicus. Methods: The aqueous and methanol leaf extracts of H. indicus were assayed for radical scavenging activity, using the stable free radical 2,2-diphenyl- 1-picryl-hydrazyl-hydrate and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid). Results: The results revealed that the IC50 values of aqueous extract of H. indicus were found to be higher than that of the other solvent extracts. The free radical scavenging activity of the plant extracts may be due to the presence of phytoconstituents. Conclusion: In all the methods, the aqueous extract has exhibited the good scavenging activity and this arises that the plant has a potential antioxidant agent.

scholarly journals Antiradical Activities and Cytotoxic Effects of Phlomis Brevibracteata Turrill on SK-HEP-1 Adenocarcinoma Cell Line

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1575
Author(s):  
Imge Kunter ◽  
Niloofar Zabib ◽  
Kelly Randmae ◽  
Mahshid Dabeshlim ◽  
Muberra Kosar
Keyword(s):  
Cell Line ◽  
Mtt Assay ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Endemic Plant ◽  
Cytotoxic Effects ◽  
Phenolic Extract

The present study investigates the total phenol amount, radical scavenging and anticancer activity of methanolic extract of Phlomis brevibracteata Turrill (PBT) leaves; an endemic plant of North Cyprus. The antiradical activity and cytotoxicity of 70% methanolic extract of PBT leaves were analyzed by the implementation of DPPH, ABTS radical scavenging activity tests and MTT assay, respectively. Our results showed significant antiradical scavenging activity in the total phenolic content of the PBT leaves. MTT assay proved potent cytotoxic activity against SK-HEP-1 cell line not only for 70% methanolic extract of PBT, but also for gallic acid and caffeic acid constituents of the total phenolic extract.

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