scholarly journals Phytochemical Screening and in vitro Antioxidant Properties of Methanol and Aqueous Leaf Extracts of Geophila obvallata

2018 ◽  
pp. 1-11
Author(s):  
Iserhienrhien Lucky Osafanme ◽  
Okolie Paulinus Ngozi
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Scientific Basis ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Reducing Ability

Aim: This study investigated the phytochemical constituents and in vitro antioxidant properties of methanol and aqueous leaf extracts of Geophila obvallata using standard methods. Materials and Methods: The in vitro antioxidant assays carried out were 1, 1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging ability, Nitric oxide (NO•) radical scavenging activity assay, 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) radical cation scavenging assay, ferric reducing properties and hydroxyl radical scavenging assays. Results: Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, steroids, saponins, terpernoids and cardiac glycosides in both extracts. Relative to the aqueous extract, the methanol extract contained a higher amount of the secondary metabolites. However, both extracts exhibited appreciable and dose-dependent capacities for quenching DPPH, ABTS•+ and NO• free radicals, and potent ferric reducing ability to levels comparable to those of ascorbic acid. The crude methanol extract showed significantly increased (P<0.05) antioxidant activity than the aqueous extract. Conclusion: It was concluded that the extract possesses strong antioxidant properties due to its content of phytochemicals, and provides scientific basis for its ethno medicinal applications.

In vitro antioxidant activity and phytochemical analyses of Acalypha godseffiana (Euphobiaceae) leaf extracts

2021 ◽  
Vol 25 (1) ◽  
pp. 75-79
Author(s):  
S.O. Olubodun ◽  
G.E. Eriyamremu ◽  
M.E. Ayevbuomwan ◽  
C.I. Nzoputa
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
In Vitro Antioxidant Activity

The presence of various bioactive components makes it necessary to analyse plants for their potential to act as a source of useful treatments and cures for many inflammatory, infectious and pathogenic diseases. This study was carried out to determine phytochemicals and in-vitro antioxidant activities of the leaf extracts of Acalypha godseffiana. The leaves of A. godseffiana were collected, dried, pulverized and extracted separately with methanol and water using maceration method. The extract was concentrated in vacuo with rotary evaporator at 40oC. The extracts were subjected to quantitative phytochemical analysis and different anti-oxidant analytical procedures like FRAP, DPPH etc to determine the radical scavenging capabilities. The results of phytochemical analysis estimated the quantities and revealed the presence of alkaloids, flavonoids, tannins, saponins and terpenoids which varied in both extracts. The methanol and aqueous extracts exhibited antioxidant activities with relatively high IC50 (IC50 = 3.67 ìg/ml and 4.42ìg/ml respectively) which accounted for a low free radical-scavenging activity when compared with the reference antioxidant, vitamin C (IC50 = 1.51ìg/ml). The results of the study indicates that A. godseffiana leaf extracts contain secondary metabolites and possesses antioxidant properties.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF LEAF EXTRACTS OF GREWIA HETEROTRICHA MAST

2016 ◽  
Vol 8 (4) ◽  
pp. 68 ◽  
Author(s):  
B. Usha ◽  
K. C. Pushpalatha
Keyword(s):  
Aqueous Extract ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts

Objective: To investigate the presence of phytochemical components and to evaluate the in vitro antioxidant activity of pet. ether, chloroform, methanol and aqueous extracts of Grewia heterotricha mast leaves.Methods: The leaves of Grewia heterotricha mast were dried and extracts were prepared using a pet. ether, chloroform, methanol by soxhlet extraction method. The aqueous extract was prepared using distilled water by cold extraction method. The preliminary phytochemical analysis was carried out on aqueous, methanol, chloroform and pet. either leaf extracts of the plant using standard qualitative procedures. The total phenolic content (TPC) was estimated using modified Folin-Ciocalteau method, tannin content by Folin-Denis method and total flavonoids by aluminum chloride method. In vitro, antioxidant activities were evaluated by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, H2O2 scavenging activity and FRAP assay.Results: The preliminary phytochemical analysis revealed the presence of complex bioactive constituents like phenols, tannins, alkaloids, terpenoids, flavonoids, saponins, steroids, glycosides, coumarins, proteins and carbohydrates. Methanolic extract showed highest total phenolic content (87.58±2.52 mg CE/g) than aqueous extract (78.46±5.36 mg CE/g). Higher tannin content was found in the aqueous extract (148.0±8.96 mg TAE/g). Total flavonoids were highest in chloroform extract (314.9±25.06 mg QE/g) followed by aqueous (242.98±32.42 mg QE/g) and methanolic extract (217.0±18.32 mg QE/g) and lowest in a pet. ether extract (188.86±23.35 mg QE/g). The methanolic extract had shown very significant DPPH radical scavenging activity (IC50 98.95 µg/ml) and H2O2 scavenging activity (IC50110.1µg/ml) compared to the standard ascorbic acid. Higher reducing ability was observed in methanol extract (131.8±11.67 mg AE/g).Conclusion: The results obtained reveal that the leaves of Grewia heterotricha mast have potent antioxidant property. The observed activity may be associated with bioactive components like phenolics, flavonoids present in the leaf extracts and could have greater importance as therapeutic agents in oxidative stress-related degenerative diseases. Further studies are needed in order to purify bioactive compounds responsible for the antioxidant property.

Strong antihemolytic and antioxidant properties of aqueous extract from Algerian Hammada elegans (Bge.) Botsch (Chenopodiaceae)

2021 ◽  
Vol 17 ◽  
Author(s):  
Brahim Asseli ◽  
Reguia Mahfoudi ◽  
Amar Djeridane ◽  
Mohamed Yousfi
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Condensed Tannin ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Vitro Assay

Background: Research on medicinal plant antioxidants has emerged as a potential therapeutic to prevent free radical generated damage in the human body. Hammada elegans Botsch (popularly known as “Ajram”) is a xerophytic plant widely found in Laghouat region, but there are only a few reports about the biological or chemical properties of these species. Hence, the aim of this study is to investigate the antioxidant and the antihemolytic activities of hexanic, acetonic, methanolic and aqueous extracts of aerial parts of Algerian Hammada elegans Botsch by employing different in vitro assay systems. Methods: The total phenolic content, the flavonoid content and the condensed tannin amount were analyzed using Folin-Ciocalteu, aluminum chloride and vanillin assays, respectively. The in vitro antioxidant capacity of extracts was assessed by CUPRAC, iron chelating, ABTS•+and antihemolytic assays, and was expressed as EC50 values. Results: Among the analyzed extracts, the aqueous extract had the highest phenolic, flavonoid and tannin contents. Also, this extract displayed the highest antioxidant capacities compared to the other extracts and standards. Its EC50 value for ABTS radical-scavenging activity was 0.265 ± 0.003 mg/L. Moreover, this extract showed high iron (II) chelating ability (EC50 = 0.958 ± 0.001 mg/L), and good antioxidant activity in the cupric ion reducing activity (CUPRAC) in a concentration dependent manner (EC50 were 0.709 ± 0.002 mg/L). Additionally, this extract had the best antihemolytic activity against AAPH-induced hemolysis (EC50=0.090 ± 0.004 mg/L). Conclusion: Our study revealed that the aqueous extract of Hammada elegans Botsch, is a potential source of antioxidants which possess a high protective effect of membrane against free radical.

scholarly journals In-vitro Antioxidant Activity and Preliminary Phytochemical Analysis of Different Leaf Extracts of Hemionitis arifolia

2021 ◽  
pp. 281-291
Author(s):  
Rajendran Raja Priya ◽  
N. Bhadusha ◽  
Veramuthu Manivannan ◽  
Thanthoni Gunasekaran
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Hydroalcoholic Extract ◽  
Reducing Power Assay

Objective: To evaluate the preliminary phytochemical content and antioxidant potential of the hydroalcoholic leaf extracts of Hemionitis arifolia. Methods: Total phenolic, flavonoid and alkaloid contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the leaf hydroalcoholic extract were evaluated against DPPH+, ABTS+, Reducing power assay and nitric oxide assay were determined. Results: The hydroalcoholic concentrate of H. arifolia uncovered the most elevated polyphenol content when contrasted and the other phytoconstituents. Absolute phenol content of the hydroalcoholic separate was observed to be 31.78%, flavonoid content is 1.02% and Alkaloid content is 30.40% individually. The Solvent concentrates showed huge cell reinforcement movement, with hydroalcoholic extract. ABTS Assay, DPPH assay, Reducing power assay and Nitric oxide assay where the Inhibition concentration were 667.75µg/ml, 734.25 µg/ml, 791.58 µg/ml and 899.67 µg/ml. Conclusion: This study suggests that hydroalcoholic leaf extracts of H. arifolia could be a potential source of natural antioxidant and justifies its use in ethno-medicine.

scholarly journals Phenolic Content and DPPH Radical Scavenging Activity of the Flowers and Leaves of Trifolium repens

2013 ◽  
Vol 8 (1) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Agnieszka Kicel ◽  
Maria Wolbiś
Keyword(s):  
Trifolium Repens ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
In Vitro Test ◽  
Light Spectrum ◽  
Leaf Extracts ◽  
Plant Materials

In order to facilitate the quality control of Trifolium repens flowers and leaves, a RP-HPLC method with UV detection was developed for the simultaneous quantitative determination of flavonols and isoflavones. The total flavonoid and phenolic (TPC) contents were determined spectrophotometrically in a visible part of the light spectrum at 425 and 760 nm, respectively. Additionally, evaluation of the antioxidant properties of the plant materials was performed using the DPPH in vitro test. The results showed that the flowers are the richest source of phenolics ranging from 28.7 to 38.8 mg GAE/g, and flavonoids, calculated for hyperoside, up to 20 mg HP/g, which hydrolyzed mainly to flavonols (the quercetin level greater than 6 mg/g). T. repens is poor in isoflavones; similar quantities of ca. 0.2 mg/g were detected in the flowers and leaves. The flower and leaf extracts showed antioxidant activity towards DPPH with EC50 values ranging from 72.3 to 179.3 μg/mL. Significant linear correlations were found between antioxidant potentials of the studied plant materials and total phenolic and flavonoid contents determined by HPLC and spectrophotometric methods ( R2 in the range of 0.97 - 0.99).

scholarly journals Evaluation and Elucidation of Bioactive Compounds from Leptogium rivurale Through in-vitro and in-silico Studies

2020 ◽  
Vol 2 (1) ◽  
pp. 10
Keyword(s):  
Aqueous Extract ◽  
In Silico ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Docking Simulation ◽  
Bioactive Constituents ◽  
Drug Candidates ◽  
In Silico Studies

Leptoguium rivurale is a flooded jelly skin lichen, the surface of which becomes jelly when wet. It is a cyanolichen in which the photosynthetic partner is a cyanobacterium in the genus name Nostac. The present study was performed to investigate the bioactive constituents of Leptoguium rivurale. Samples were collected from forest regions of Kodaikanal. The collected Lichens were washed and dried and then extracted by using methanol and distilled water. Qualitative analysis of phytochemicals from the extracts showed the presence of carbohydrates, glycosides, phenols, terpenoids, saponins, and proteins. The amount of carbohydrates, phenols, and proteins was found to 350u/g and 380u/g, 200u/g, and 240u/g and 490u/g and 320u/g in aqueous and methanol extracts. The α-amylase enzyme inhibition assay ranged from 47.2% to 58.4% for methanol extract and from 35.12% to 51.1% for aqueous extract. The DPPH radical scavenging activity ranged from 27.6% to 49.8% for methanol extract and from 21.3% to 42.2% for aqueous extract. The anti-inflammatory activity ranged from 40.5% to 86.2% for methanol extract and from 49.4% to 79.2% for aqueous extract. Then the extract was given for GC-MS analysis. The molecules that were obtained in this analysis were used as ligands and in-silico molecular docking simulation was made using AutoDock software through which it was found that out of the docked ligands cyclohexanol and oxirane were the potential drug candidates.

scholarly journals Phytochemical Profiling, Antioxidant and Antibacterial Activities of Leaf Extracts From Rhus leptodictya

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
Fanyana M. Mtunzi ◽  
Ikechukwu P. Ejidike ◽  
Tshifhiwa Matamela ◽  
Ezekiel Dikio ◽  
Michael J. Klink
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Focal Point ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Active Compounds

An integral part of nature is herbal plants which contain natural substance essential to promote health. The focal point of this research resides on the phytochemical, antioxidant and antibacterial investigations of Rhus leptoditya leaves. Hexane, dichloromethane, ethyl acetate, acetone, and methanol solvent extracts were tested for their antioxidant and antimicrobial properties. Phytochemical analysis of R. leptodictya extracts revealed the presence of phenols, tannins, saponins, and flavonoids. Acetone extract with total flavonoid contents of 18.22 ± 2.1 mg/g while methanol extract with phenol contents (0.38 ± 0.002 mg/g) exhibited the highest amount amongst the studied extracts in the preliminary phytochemicals screening. The antioxidant activity of the plant extracts was evaluated using TLC based standard qualitative 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The methanol and acetone extracts showed maximum antioxidant bands compared to other selected solvents and promising radical scavenging activity at par with the standard antioxidant ascorbic acid (IC50 = 0.125 ± 0.013 mg/ml). The bioautography assay also demonstrated the bacterial growth inhibition of active compounds present in leave extracts. Acetone and ethyl acetate fractions showed the highest number of antibacterial active compounds against S. aureus. Minimum inhibitory concentration of the extracts examined using microplates of 96 wells containing Muller-Hinton methods against some bacterial strains, revealed methanol and acetone fractions with the lowest MIC value of 0.04 mg/ml against S. pneumoniae, while methanol extract possesses 0.008 mg/ml against S. aureus and B. subtilis. Results from this study project the R. leptodictya leave extracts constituents as health supplements that could be useful in food industries and in the treatment of various infectious diseases.

scholarly journals Antioxidant and phytochemical analysis of volatile oil and extracts of Pinus wallichiana

2019 ◽  
Vol 4 (2) ◽  
pp. 37-40
Author(s):  
Pooja Sharma ◽  
Sahil Gupta ◽  
Nazli Bhatt ◽  
Shajaat Hussain Ahanger ◽  
Divya Gupta ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aqueous Extract ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Chelating Activity ◽  
Pinus Wallichiana

The present study aims to explore the chemical constituents and antioxidant potential of Pinus wallichiana essential oil and extracts. Chemical composition of the essential oil was analyzed by GC-MS and result showed that the oil contain three major monoterpene compounds viz. α-pinene (48.6%), β-pinene (45.6%) and limonene (5.6%). Antioxidant potential of Pinus wallichiana was analyzed by three basic methods; DPPH radical scavenging method, FRAP assay and Fe2+ ion chelating activity. Among leaf and bark extracts, best radical scavenging, activity was determined by aqueous extract (IC50 values 20.83±0.8µg/ml) of bark followed by its methanol extract (IC50 value 25.9±1.6µg/ml). The aqueous extract of bark also exhibited better reducing and chelating activity than leaf extracts. Essential oil showed moderate radical scavenging and chelating activity but negligible reducing activity. Phytochemical analysis of extracts determined that the aqueous and methanol extracts of bark contain rich amount of poly phenol and flavonoids, in comparison to the leaf extracts. A significant correlation between the antioxidant activity and these phytoconstituents of the extracts has been observed. The results of the present study concluded that the bark of Pinus wallichiana is a potential source of active antioxidant constituents that could be further explored and exploited for numerous commercial applications.

scholarly journals Nutritional Composition and Phytochemical, Antioxidative, and Antifungal Activities ofPergularia tomentosaL.

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Imen Lahmar ◽  
Hafedh Belghith ◽  
Ferjani Ben Abdallah ◽  
Karima Belghith
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Nutritional Composition ◽  
Total Phenolic ◽  
Natural Material ◽  
Leaf Extracts ◽  
Methanolic Extracts

Crude extracts from a medicinal Tunisian plant,Pergularia tomentosaL., were the investigated natural material. Butanolic extract of roots analyzed with IR spectra revealed the presence of hydroxyl, alcoholic, and carboxylic groups and sugars units. Analysis of some secondary metabolites, total phenolic, flavonoids, flavonols, and procyanidins, was performed using different solvents following the increased gradient of polarity. Fruits and leaves contained the highest amounts of all these compounds. Antioxidant properties were evaluated by the determination of free radical scavenging activity and the reducing power of methanolic extracts. Fruits and leaf extracts were the most powerful antioxidants for the two-assay in vitro system. Stems and fruits extracts exhibit an antifungal activity againstFusarium oxysporum f. sp. lycopersiciwhich could become an alternative to synthetic fungicide to controlSolanumspecies fungal diseases.

scholarly journals Chemical profiling, in vitro antioxidant, membrane stabilizing and antimicrobial properties of wild growing Murraya paniculata from Amarkantak (M.P.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shruti Sonter ◽  
Shringika Mishra ◽  
Manish Kumar Dwivedi ◽  
Prashant Kumar Singh
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Phytochemical Analysis ◽  
Hypotonic Solution ◽  
High Concentration ◽  
Leaf Extracts ◽  
Traditional Use ◽  
Murraya Paniculata

AbstractThe excessive usage of antibiotics in humans and veterinary medicine has lead to the emergence of antibiotic resistance and now requires the use of novel antibiotics. There has been increased interest towards plants as source of drugs because of their pharmacological potency and long traditional usage. The aim of the current study was to evaluate bioactive components, antioxidant, and anti-inflammatory activities of the leaf extracts of Murraya paniculata, a plant traditionally used in Indian medicinal system. Evaluations were made for phytochemical analysis, antioxidant, membrane stabilizing, and antimicrobial activities. The methanol extract displayed the highest flavonoid and phenolic content, the acetone extract demonstrated considerable ABTS inhibitory activity (IC50value:555.18 ± 1.68 µg/mL) and the hexane extract exhibited highest H2O2 radical scavenging activity (IC50value: 509.84 ± 3.03 µg/mL). The aqueous extract displayed 19.4 ± 0.66% RBC hemolysis and 80.5 ± 0.66% protection caused by hypotonic solution at high concentration of the extract. The fractions of hexane extract revealed a higher zone of inhibition than crude extract. The major components found in the fractions were cyclohexane (40.11%) and 3-(6-Methoxy-3-methyl-2-benzofuranyl) Cyclohexanone (13.68%) as analyzed by GC–MS/MS technique. The current results validate the traditional use of the M. paniculata and warrant its potential in drug development programs in further investigations.

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