scholarly journals PHYTOCHEMISTRY AND BIOLOGICAL ACTIVITIES OF CRATEVA ADANSONII EXTRACTS

2018 ◽  
Vol 10 (9) ◽  
pp. 62
Author(s):  
Martial S. Nounagnon ◽  
Durand Dah-nouvlessounon ◽  
Christine N’tcha ◽  
Boris Legba ◽  
Farid Baba-moussa ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Reducing Power ◽  
Artemia Salina ◽  
Diffusion Method ◽  
Clinical Strain ◽  
Active Principle ◽  
Phytochemical Screening ◽  
Dpph Method ◽  
Dose Dependent

Objective: This study aims to search the secondary metabolites of Crateva adansonii (leaves and bark) and evaluate some biological activities (antimicrobial, antioxidant and cytotoxicity’s property) of these extracts.Methods: The phytochemical screening was made with standard method. Agar diffusion method was used for antibacterial activity coupled with Minimum Inhibitory Concentration (MIC) and Bactericidal (MBC) determination. The antifungal test was performed by the mycelial development reduction method. DPPH method was used to evaluate the extracts antioxidant activity. Artemia salina larvae were use as support to evaluate the toxicity of the extracts.Results: The results of the phytochemical screening reveal the presence of polyphenols, triterpenoids, and glycosides in the both organs (leaves and bark) of C. adansonii. All the extracts have low activity on the reference strains with a larger diameter of 17±00 mm (S. oralis). Only the ethanolic and hydroethanolic leaf’s extracts inhibited respectively 20% and 10%, of the clinical strain S. aureus. The ethyl acetate leaf’s extract shows the best antifungal activity (89,19%) with A. clavatus. All extracts present dose-dependent antiradical properties and are non-toxic for the cells of Artemia salina. C. adansonii bark extracts had the best reducing power (23,80±0, 137 mmol/g EqAA EAA) of the DPPH radical.Conclusion: C. adansonii is therefore an active principle source for the development of drugs to antimicrobial and antioxidant activities

scholarly journals Evaluation of Phytochemical Screening, Antimicrobial and Antioxidant Activities of Ethanol Extracts of Cucumis flexouses and Cucumis reticulatus Seeds

2021 ◽  
Vol 41 (01) ◽  
pp. 142-146
Author(s):  
Hafiza Tuseef Sayyar
Keyword(s):  
Antioxidant Activities ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Dpph Method ◽  
Antioxidant Effects

The current study is designed to investigate the phytochemical screening and to examine the potential antimicrobial, antioxidant activities of ethanol extract of Cucumis flexouses and Cucumis reticulatus. The ethanol extract of C. flexouses and C. reticulatus seeds were subjected to preliminary phytochemical screening for the confirmation of various phytochemicals and their total phenolic and flavonoid content is verified by the colorimetric method. Subsequently, antimicrobial activity of both extracts at the concentration of (25, 50, 100 mg/ml) against various microbial organisms was evaluated via the disc diffusion method by measuring zone of inhibition and estimating minimum inhibitory concentration (MIC). The antioxidant activity was assessed by ferric thiocyanate (FTC) and DPPH free radical scavenging method. The ethanol extract of Cucumis flexouses showed maximum antibacterial activity against gram-positive bacteria including Staphylococcus aureus (29.0±0.05mm), Bacillus subtilis (17.0±0.02 mm) followed by gram-negative bacteria Escherichia coli (22.0±0.05 mm) and Pseudomonas aeruginosa (15.04±0.34mm) respectively at 100mg/ ml concentration as compared to standard drug. Moreover, both the extracts showed no activity against fungal species Candida albicans. Similarly, significant antioxidant effects at 100mg/ml concentration (1.17±0.025) were also observed by FTC and (1.09±0.017) by DPPH method. The result of the current study depicts that ethanol extract of C. flexouses seeds possess significant in-vitro antimicrobial and antioxidant effects as compared to C. reticulatus seed extract and could be a good substitute for many infectious diseases

Comparative analysis of antioxidant activity and phenolic compounds in the fruits of Aronia spp.

10.5219/1360 ◽  
2020 ◽  
Vol 14 ◽  
pp. 393-401
Author(s):  
Yulia Vinogradova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Eva Ivanišová ◽  
Ján Brindza
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Reducing Power ◽  
Biologically Active ◽  
Polyphenol Compounds ◽  
Dpph Method ◽  
Ethanol Extracts

Chokeberry (Aronia Medik.) is a non-traditional fruit plant known as a rich source of biologically active compounds and inhibits the numerous biological activities. We compared the antioxidant activity and phenolic compounds of fruits between widely cultivated Aronia mitschurinii (AM-TCH, from Tchekhov district; AM-D, from Dmitrov district; AM-OZ, from Orekhovo-Zuevsky district of Moscow region, Russia) and introduced North American Aronia species (Aronia arbutifolia (AA-M), A. melanocarpa (AML-M), A. × prunifolia (AP-M), which have not been planted yet in the arboretum of Main Botanical Garden of the Russian Academy of Sciences (Moscow). Studying samples were collected in their secondary distribution range. Ethanolic extracts were determined for antioxidant capacity (antioxidant activity by DPPH and phosphomolybdenum methods, the total content of polyphenols, flavonoids, phenolic acids) and measured spectrophotometrically. As standards were used Trolox (TE) for antioxidant activities, gallic acid (GAE) for polyphenol content, quercetin (QE) for flavonoid content, caffeic acid (CAE) for phenolic acid content. The antioxidant activity by DPPH method in ethanol extracts of investigated plants was from 6.96 (AM-D) to 8.89 (AM-OZ) mg TE.g-1 DW. Reducing the power of investigated extracts exhibited activity from 151.47 (AM-OZ) to 297.8 (AA-M) mg TE.g-1 DW. The content of polyphenol compounds determined from 25.98 (AM-TCH) to 54.39 (AA-M) mg GAE.g-1 DW, phenolic acids content was from 7.76 (AP-M) to 11.87 (AM-D) mg CAE.g-1 DW and the content of flavonoids detected from 8.12 (AM-OZ) to 16.62 (AM-D) mg QE.-1 DW. Obtained data showed a strong correlation between the content of polyphenol compounds and reducing the power of extracts (r = 0.700), between flavonoids and phenolic acids (r = 0.771) and also between phenolic acids and reducing power (r = 0.753) in Aronia ethanol extracts. Fruits of investigated species of Aronia can be propagated as a source of polyphenol compounds with antioxidant activity and obtained results may use for farther pharmacological study.

scholarly journals DETERMINATION OF BIOLOGICAL ACTIVITIES OF THREE MARINE ALGAE COLLECTED FROM VISAKHAPATNAM COAST

2017 ◽  
Vol 10 (12) ◽  
pp. 274 ◽  
Author(s):  
Satya Lakshmi S
Keyword(s):  
Antioxidant Activity ◽  
Marine Algae ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Dpph Method ◽  
Anti Inflammatory

  Objective: Determination of biological activities of marine algae collected from the Visakhapatnam coastal region.Methods: Antibacterial activity of algal extracts determined by the well diffusion method, antioxidant activity was determined by reducing power (RP) method and 1, 1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging method finally anti-inflammatory activity was determined by human red blood cell stabilization method and egg albumin method.Results: Methanolic extracts of three algae have potential inhibition activity against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. Green alga Enteromorpha compressa has better antioxidant activity compared to the Gracilaria arcuata and Ulva fasciata when tested in RP and DPPH method. U. fasciata found good anti-inflammatory activity among the selected three algae.Conclusion: The three tested algae exhibited significant antibacterial and antioxidant activity compared to anti-inflammatory activity. These bioactive compounds containing macroalgae may find their commercial potential in medicine, food, and cosmetic industry

scholarly journals Phytochemical Screening and Biological Activities of Combretum adenogonium Leaves Extracts

2017 ◽  
Vol 13 (30) ◽  
pp. 358
Author(s):  
Martial S. Nounagon ◽  
Durand Dah-Nouvlessounon ◽  
Christine N’tcha ◽  
Sani M. Akorede ◽  
Haziz Sina ◽  
...  
Keyword(s):  
Biological Activities ◽  
Scientific Journal ◽  
Meat Products ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Dpph Method

Combretum adenogonium is beniniens pharmacopoeia medicinal plant used for the treatment of various diseases. This work aims to study the phytochemical and assess some biological activities of C. adenogonium leaves extracts. The phytochemical analysis (qualitative et quantitative) was conducted by standard analytical chemistry method. Antioxydant activity was evaluated by the DPPH method. Antibacterial activity was evaluated in vitro with 10 references strains , 10 Staphylococcus strains isolated from European Scientific Journal October 2017 edition Vol.13, No.30 ISSN: 1857 – 7881 (Print) e - ISSN 1857- 7431 359 meat products and 10 clinical Staphylococcus aureus strains isolated from Buruli ulcer lesions and pus. The Minimum Inhibitory Concentration (MIC) and Bactericidal (CMB) were determined by macrodilution method. The extracts cytotoxic effect was evaluated with Artemia salina larvae. The phytochemical screening revealed the presence of flavonoids, tannins, anthocyanins, saponin and triterpenoids. The methanolique extract present the higest content (450.66 ± 0.004 µg EAG/mg) of total polyphenolic compound. The results showed the good antioxidant activity. The inhibitory diameter zone vary (p < 0.001) according to the strains. The largest medium inhibitory diameter (21.85 ± 0.17 mm) was obtained with the ethanolic extract, while the lowest (6.00 ±0.00 mm) were recorded with water-ethanol extract. The variation between CMI and CMB is not significant (p > 0.05). The higher LD50 (27.66 mg/ml) was recorded with methanol extract. The results of this study confirm some use of C. Adenogonium extracts. But; these extracts must be use with moderation

scholarly journals Evaluation of antioxidant activity and toxicity in Artemia salina of the ether extract and fractions from Tecoma stans seeds

2021 ◽  
Vol 10 (9) ◽  
pp. e12510917829
Author(s):  
Lucas Santos Azevedo ◽  
Thaís Paula Rodrigues Gonçalves ◽  
Paula Avelar Amado ◽  
Luciana Alves Rodrigues dos Santos Lima
Keyword(s):  
Antioxidant Activity ◽  
Petroleum Ether ◽  
Biological Activities ◽  
Artemia Salina ◽  
Ether Extract ◽  
Phytochemical Screening ◽  
Soxhlet Apparatus ◽  
Dpph Method ◽  
Potential Applications ◽  
Esterification Reactions

Tecoma stans (L.) Juss. Ex Kunth is a species belonging to the Bignoniaceae family, popularly known as yellowing, yellow bell, and garden little ipe. Studies with T. stans seeds are scarce and it have potential in the search for natural compounds with biological activities. This study aimed to evaluate the toxicity and antioxidant activity of the ether extract (EE) and fractions from T. stans seeds. The EE was obtained in Soxhlet apparatus with petroleum ether and fractions were obtained by hydrolysis and esterification reactions. Phytochemical screening evaluated the presence of steroids, triterpenoids, alkaloids, coumarins and anthraquinones in EE. The toxicity was evaluated by the A. salina lethality test and antioxidant activity by DPPH method. It was observed the presence of steroids, triterpenoids and alkaloids in EE, which had no toxicity to A. salina (LC50 > 1000 µg/mL). The fractions of seeds exhibited toxicity on A. salina. All samples showed antioxidant activity with EC50 between 0.9 and 1.1 µg/mL. These results indicated potential applications for T. stans seeds.

Chemical Constituents Isolated from Rhododendron ungernii with Antioxidant Profile

2019 ◽  
Vol 9 (3) ◽  
pp. 238-243 ◽  
Author(s):  
Emine Dede ◽  
Nusret Genc ◽  
Mahfuz Elmastas ◽  
Huseyin Aksit ◽  
Ramazan Erenler
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Isolation And Identification ◽  
Reduced Pressure ◽  
Scavenging Effect ◽  
Pharmaceutical Industries ◽  
Dpph Scavenging

Background: Plant in Rhododendron genus that contains phenolic compounds has been used in traditional medicine and revealed considerable biological activities. Objective: Isolation and identification of antioxidant natural products from Rhododendron ungernii. Methods: Rhododendron ungernii Trautv. flowers were collected and dried in shade. The dried flowers were extracted with methanol for 3 days. The solvent was removed by reduced pressure to yield the extract which was subjected to column chromatography (Sephadex LH-20, C18 reversed phase column) to isolate catechin-7-O-glucoside (1), quercetin-3-O-β-galactoside (2), quercetin-3-O- β-xyloside (3), farrerol (4), myricetin (5), and quercetin (6). The structures of isolated compounds were elucidated by spectroscopic methods such as 1D-NMR, 2D-NMR, and LC-TOF/MS. DPPH scavenging effect, ABTS+ scavenging activity, and reducing power (FRAP) were performed for antioxidant assays of isolated natural compounds. Results: Isolated flavonoids displayed the outstanding antioxidant activities. Catechin-7-O-glucoside (1) and quercetin-3-O-β-galactoside (2) (IC50, 3.66 µg/mL) had the most DPPH• scavenging effect among the compounds. The highest ABTS•+ scavenging activity (IC50, 1.41 µg/mL) and reducing power effect (6.05 mmol TE/g comp) were observed for myricetin (5). Conclusion: R. ungernii extract and isolated compounds could be a promising antioxidant for food and pharmaceutical industries.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

PHYTOCHEMICAL SCREENING AND PHARMACOLOGICAL EVALUATION OF AEGLE MARMELOS FRUIT

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (04) ◽  
pp. 59-64
Author(s):  
Pratibha Thaware ◽  
Pushpa Karale ◽  
Mahesh Karale ◽  
Pranati Chavan
Keyword(s):  
Methanolic Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Pharmacological Action ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Aegle Marmelos ◽  
Pharmacological Screening ◽  
Hplc Data

The objective of this study was to evaluate the phytochemical and pharmacological screening of fruit extracts of Aegle marmelos using various in vitro methods. The antioxidant activity of the extracts was evaluated by using DPPH free radical scavenging and reducing power method, while well diffusion method was used for antimicrobial activity. Phytochemical screening indicated the presence of flavonoids, and phenolics were abundantly present in methanolic and aqueous extracts of A. marmelos. Methanolic extract showed significant antioxidant potential, which was however inferior to that of ascorbic acid. The methanolic extract showed maximum zone of inhibition against the S. aureus (18 mm) and it was higher than that of the standard tetracycline. The HPLC data predict that the presence of scopoletin, marmelosin and umbelliferone, which may contribute to their pharmacological action.

scholarly journals Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Kenza Ammor ◽  
Dalila Bousta ◽  
Sanae Jennan ◽  
Bahia Bennani ◽  
Abdellah Chaqroune ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Antioxidant Power ◽  
Negative Effect ◽  
Hydroethanolic Extract

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

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